##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936839_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 425347 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.844510482029968 31.0 31.0 34.0 30.0 34.0 2 31.856293802471864 31.0 31.0 34.0 30.0 34.0 3 32.00433058185435 33.0 31.0 34.0 30.0 34.0 4 35.81105074209998 37.0 35.0 37.0 35.0 37.0 5 35.723677374002875 37.0 35.0 37.0 33.0 37.0 6 35.7796551991668 37.0 35.0 37.0 33.0 37.0 7 35.720715086740945 37.0 35.0 37.0 33.0 37.0 8 35.70927031341469 37.0 35.0 37.0 33.0 37.0 9 37.30822363858215 39.0 37.0 39.0 34.0 39.0 10-11 37.24906958318738 39.0 37.0 39.0 33.0 39.0 12-13 37.18359363061218 39.0 37.0 39.0 33.0 39.0 14-15 38.22774464143394 40.0 37.5 41.0 33.0 41.0 16-17 38.11513540709115 40.0 37.0 41.0 33.0 41.0 18-19 38.211028877598764 40.0 37.5 41.0 33.5 41.0 20-21 38.31552943831742 40.0 38.0 41.0 34.0 41.0 22-23 38.2090645990215 40.0 37.0 41.0 33.5 41.0 24-25 38.086642200368175 40.0 37.0 41.0 33.0 41.0 26-27 37.890016856825135 40.0 37.0 41.0 33.0 41.0 28-29 37.72550176679276 40.0 36.5 41.0 33.0 41.0 30-31 37.49461263392007 39.0 36.0 41.0 33.0 41.0 32-33 37.331396483341834 39.0 35.5 41.0 33.0 41.0 34-35 37.199857998293155 39.0 35.0 41.0 32.0 41.0 36-37 36.969509600396854 39.0 35.0 41.0 31.5 41.0 38-39 36.768950997656034 39.0 35.0 41.0 31.0 41.0 40-41 36.610634376168164 39.0 35.0 41.0 31.0 41.0 42-43 36.34264729738308 39.0 35.0 41.0 30.0 41.0 44-45 35.943138190700765 39.0 35.0 40.0 30.0 41.0 46-47 35.56484705428744 39.0 35.0 40.0 28.0 41.0 48-49 35.13664020199978 38.5 35.0 40.0 26.0 41.0 50-51 34.91841132063938 38.0 35.0 40.0 24.5 41.0 52-53 34.728780266464796 38.0 34.5 40.0 24.0 41.0 54-55 34.54527362365316 38.0 34.0 40.0 24.0 41.0 56-57 34.30061925909904 38.0 34.0 40.0 23.0 41.0 58-59 34.034417781246844 38.0 33.5 40.0 22.5 41.0 60-61 33.72921638097836 38.0 33.0 40.0 22.0 41.0 62-63 33.390708057186245 37.0 33.0 40.0 20.5 41.0 64-65 33.01229584315865 37.0 32.5 39.0 19.5 40.5 66-67 32.63594194857375 36.0 32.0 39.0 18.0 40.0 68-69 32.164685539101015 36.0 31.0 39.0 15.0 40.0 70-71 31.674640940220574 35.0 31.0 38.0 12.5 40.0 72-73 31.304478461115277 35.0 31.0 37.5 8.0 39.5 74-75 30.81205110180629 35.0 30.0 37.0 7.0 39.0 76-77 29.382492412077667 33.5 28.5 35.5 4.0 38.0 78-79 30.0891272302379 34.0 30.0 36.0 2.0 38.5 80-81 29.864514149623716 34.0 30.0 36.0 2.0 37.5 82-83 29.47207574051304 34.0 29.0 35.0 2.0 37.0 84-85 29.029843868653124 34.0 29.0 35.0 2.0 36.5 86-87 28.664886551450934 34.0 29.0 35.0 2.0 36.0 88-89 28.238331291862877 34.0 29.0 35.0 2.0 36.0 90-91 27.759885458225874 33.5 27.5 35.0 2.0 35.0 92-93 27.25597100720118 33.0 26.0 35.0 2.0 35.0 94-95 26.71000383216527 33.0 25.5 35.0 2.0 35.0 96-97 26.004533945225898 32.5 24.5 34.0 2.0 35.0 98-99 25.234138244774265 32.0 21.0 34.0 2.0 35.0 100-101 22.171248415999173 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5383968583917209 1101 2 0.4888541214576918 1101 3 0.3709640876271081 1101 4 0.3624019784938284 1101 5 0.357692185752029 1101 6 0.3587199497191449 1101 7 0.34101868255363854 1101 8 0.39366914744506687 1101 9 0.583753060416484 1101 10-11 0.4914838579656262 1101 12-13 0.4189395202619721 1101 14-15 0.7041988981136811 1101 16-17 0.8085262140620344 1101 18-19 0.5099978004089678 1101 20-21 0.6490445932453142 1101 22-23 0.5224972799412413 1101 24-25 0.6551132323224707 1101 26-27 0.556791219979857 1101 28-29 0.6990738936630194 1101 30-31 0.8666274226664825 1101 32-33 0.921239579037632 1101 34-35 1.1611065998700525 1101 36-37 1.202535284602554 1101 38-39 0.6415974529722561 1101 40-41 0.5762842290985333 1101 42-43 0.7696755224964633 1101 44-45 0.622411323927679 1101 46-47 0.5791987333025901 1101 48-49 0.5081460334429408 1101 50-51 0.5797685311034115 1101 52-53 0.29894617138914015 1101 54-55 0.6140341821195818 1101 56-57 0.7065713518410917 1101 58-59 0.9807831093819175 1101 60-61 0.8784363647284223 1101 62-63 0.8992920782950193 1101 64-65 0.5361297515793026 1101 66-67 0.4697414027854805 1101 68-69 0.49249767595700433 1101 70-71 0.675433920278607 1101 72-73 0.5060594737258945 1101 74-75 0.7731489362454802 1101 76-77 0.5482013551203444 1101 78-79 0.8653751448491498 1101 80-81 1.1759820610580647 1101 82-83 1.4619928493062773 1101 84-85 1.2209385521991152 1101 86-87 1.1543131900050128 1101 88-89 0.8266523669639163 1101 90-91 1.3493730837764666 1101 92-93 1.245344442630664 1101 94-95 1.2691582411287605 1101 96-97 1.4855756391492392 1101 98-99 1.1077449205551488 1101 100-101 0.9730353423505633 1102 1 0.4305843583917195 1102 2 0.14979162145769465 1102 3 0.19544325429377096 1102 4 0.38552697849382866 1102 5 0.374046352418695 1102 6 0.2603866163858086 1102 7 0.3352895158869771 1102 8 0.35918998077840314 1102 9 0.30562806041648116 1102 10-11 0.40242135796562906 1102 12-13 0.3963353535953047 1102 14-15 0.6832093147803491 1102 16-17 0.5909741307287035 1102 18-19 0.7443207170756381 1102 20-21 0.7400862599119833 1102 22-23 0.7148931132745773 1102 24-25 0.7686548989891477 1102 26-27 0.7271558033131882 1102 28-29 0.29824056032969537 1102 30-31 0.5473565893331482 1102 32-33 0.6330624957043085 1102 34-35 0.5444920165367151 1102 36-37 0.42638945126922323 1102 38-39 0.44086828630558017 1102 40-41 0.695971729098531 1102 42-43 0.6361338558297973 1102 44-45 0.951734240594341 1102 46-47 0.6050320666359212 1102 48-49 0.4788752001096128 1102 50-51 0.34409144777008294 1102 52-53 0.43582117138914356 1102 54-55 0.5026279321195801 1102 56-57 0.518498435174422 1102 58-59 0.5875018593819163 1102 60-61 0.49140511472842263 1102 62-63 0.6762712449616899 1102 64-65 0.47409850157929867 1102 66-67 0.23317890278547537 1102 68-69 0.12879975929033094 1102 70-71 0.21366308694527802 1102 72-73 0.4915282237258971 1102 74-75 0.2152843529121462 1102 76-77 0.3298680217870107 1102 78-79 0.4870938948491492 1102 80-81 0.30504456105806455 1102 82-83 0.26537826597294156 1102 84-85 0.2815635521991169 1102 86-87 0.3999381900050132 1102 88-89 0.895194033630581 1102 90-91 1.0764043337764662 1102 92-93 0.8894590259639941 1102 94-95 0.6967624077954255 1102 96-97 0.8517214724825699 1102 98-99 0.31123450388848184 1102 100-101 0.5308999256838973 1103 1 0.09844150124886397 1103 2 0.044434478600550165 1103 3 -0.014675793325274356 1103 4 0.1595745975414502 1103 5 0.2070225428948831 1103 6 0.1700294735286647 1103 7 0.286479992077453 1103 8 0.19359474268316745 1103 9 0.17884234613077155 1103 10-11 0.3360523103465809 1103 12-13 0.3770496393095897 1103 14-15 0.47767360049464 1103 16-17 0.5780574640620415 1103 18-19 0.63741595517088 1103 20-21 0.6155624503881754 1103 22-23 0.7047145418460019 1103 24-25 0.6939525180367596 1103 26-27 0.6507272318846233 1103 28-29 0.6124667508058863 1103 30-31 0.5381303988569641 1103 32-33 0.6174077337995385 1103 34-35 0.5805634451081474 1103 36-37 0.7485918322216065 1103 38-39 0.738546857734157 1103 40-41 0.6401979195747245 1103 42-43 0.5043481415440851 1103 44-45 0.8644128120229126 1103 46-47 0.9994368285406878 1103 48-49 1.155839485823904 1103 50-51 0.668793828722464 1103 52-53 0.9178449809129532 1103 54-55 0.7000088845005337 1103 56-57 0.7398674827934713 1103 58-59 0.9683947165247702 1103 60-61 0.8830122575855697 1103 62-63 0.946985530675974 1103 64-65 0.5999318349126312 1103 66-67 0.8071669980235754 1103 68-69 1.117311664052238 1103 70-71 0.9519964202786113 1103 72-73 0.9728377475354186 1103 74-75 0.9098676862454802 1103 76-77 1.030939450358435 1103 78-79 1.1217367519920067 1103 80-81 1.1692112277247304 1103 82-83 1.416866361211035 1103 84-85 1.1249564093419728 1103 86-87 0.8764858090526317 1103 88-89 1.1940630812496273 1103 90-91 1.7620590956812272 1103 92-93 1.9342209307258997 1103 94-95 1.9431909792239956 1103 96-97 1.7746381391492356 1103 98-99 2.297960694364672 1103 100-101 2.565185639969613 1104 1 0.3382541476188905 1104 2 0.1513431437059367 1104 3 0.23821257513686334 1104 4 0.08514056163200223 1104 5 0.22907562642338064 1104 6 0.1730739700157855 1104 7 0.34225672900172555 1104 8 0.3589733531437389 1104 9 0.29500159671624715 1104 10-11 0.4233669083169147 1104 12-13 0.3311479999653244 1104 14-15 0.42931294475693704 1104 16-17 0.42827214009638936 1104 18-19 0.46635818780163163 1104 20-21 0.369465651012689 1104 22-23 0.33041711795841877 1104 24-25 0.3694599341179483 1104 26-27 0.29621903516331116 1104 28-29 0.5940046118519362 1104 30-31 0.7561241537359678 1104 32-33 0.5383874371562882 1104 34-35 0.1376477542416339 1104 36-37 0.20967692199521792 1104 38-39 0.32367566335476283 1104 40-41 0.32513156516410646 1104 42-43 0.44533174810145937 1104 44-45 0.943452624669284 1104 46-47 0.6935625116007955 1104 48-49 0.40291208535550993 1104 50-51 0.42583910584970397 1104 52-53 0.48004248286454754 1104 54-55 0.4167731311828149 1104 56-57 0.5329978496943255 1104 58-59 0.4671066603186844 1104 60-61 0.2745227962272523 1104 62-63 0.7463415025729319 1104 64-65 0.592710913757287 1104 66-67 0.5863434226918045 1104 68-69 0.3715076047235897 1104 70-71 0.4556654288656503 1104 72-73 0.8285949684565743 1104 74-75 0.5446256878067572 1104 76-77 0.14651322085024177 1104 78-79 0.12928944520043828 1104 80-81 0.26378577885665777 1104 82-83 0.36981327768254246 1104 84-85 0.27191191285485417 1104 86-87 0.6431524757192975 1104 88-89 0.7066899352699245 1104 90-91 0.7130934438467236 1104 92-93 0.9933349041841382 1104 94-95 1.5808958972567844 1104 96-97 1.59538365046852 1104 98-99 1.3008100308205641 1104 100-101 1.2216844690094248 1105 1 0.17445228292002213 1105 2 0.12078218749542913 1105 3 0.21407532976547117 1105 4 0.26599867660703325 1105 5 0.20050861656963548 1105 6 0.11618850317826457 1105 7 0.06925178003791643 1105 8 0.1519729996463255 1105 9 0.14359975852968887 1105 10-11 0.23367135796562621 1105 12-13 -0.04357030678205831 1105 14-15 0.2737989374218657 1105 16-17 0.19519582884191067 1105 18-19 0.16583015103790189 1105 20-21 0.045369278779908484 1105 22-23 0.3742563208217433 1105 24-25 0.38103697446084084 1105 26-27 0.23866523727546252 1105 28-29 0.303830182971204 1105 30-31 0.3866254572576793 1105 32-33 0.3146898541948673 1105 34-35 0.3666146580461529 1105 36-37 0.32424322485412915 1105 38-39 0.34919375800370034 1105 40-41 0.09618399324947546 1105 42-43 0.18467159167885683 1105 44-45 0.35548424059434325 1105 46-47 0.7745603685227209 1105 48-49 0.9727195397322532 1105 50-51 1.0569452213549937 1105 52-53 1.188981548747627 1105 54-55 1.0594203849497745 1105 56-57 1.1875314540423432 1105 58-59 1.3468650669290838 1105 60-61 1.3194003977472875 1105 62-63 1.1893372826975366 1105 64-65 1.0696645393151485 1105 66-67 1.0468345631628395 1105 68-69 0.9354271177808968 1105 70-71 0.7066347850584833 1105 72-73 0.6645942614617475 1105 74-75 0.6614400132895035 1105 76-77 0.5297265123530472 1105 78-79 0.18841464956613052 1105 80-81 0.6277804101146671 1105 82-83 0.7860150584257717 1105 84-85 0.7324361937085513 1105 86-87 0.7100325296276537 1105 88-89 0.5207364864607662 1105 90-91 1.055814711134957 1105 92-93 0.6388929882281467 1105 94-95 1.1373284455312742 1105 96-97 1.0761318498410617 1105 98-99 0.8400316736998015 1105 100-101 1.348305586061258 1106 1 0.4004297192164614 1106 2 0.24038440496284608 1106 3 0.3721185120257289 1106 4 0.2781558444732113 1106 5 0.31551542458364423 1106 6 0.388144348344575 1106 7 0.2164750828972828 1106 8 0.3950920426340758 1106 9 0.6905507408288543 1106 10-11 0.4901146569347006 1106 12-13 0.43695391029633157 1106 14-15 0.8167015828215867 1106 16-17 0.5810643369142738 1106 18-19 0.4959702016117191 1106 20-21 0.7034367753758985 1106 22-23 0.6336173400787004 1106 24-25 0.7388224247623398 1106 26-27 0.7775681744472109 1106 28-29 1.0116812819791718 1106 30-31 0.8832457645908818 1106 32-33 0.6027661039517227 1106 34-35 0.4561157278769201 1106 36-37 0.25217553374344703 1106 38-39 0.5560358120787825 1106 40-41 0.4541031723975024 1106 42-43 0.5464689073761946 1106 44-45 0.545561560181973 1106 46-47 0.25789289137819793 1106 48-49 0.04280561248074832 1106 50-51 0.03191361271854021 1106 52-53 0.3219809652035721 1106 54-55 0.39814339603710636 1106 56-57 0.21673297125688862 1106 58-59 0.24089103463964534 1106 60-61 0.14015511472842235 1106 62-63 0.4571475336214803 1106 64-65 0.5256191201360068 1106 66-67 0.49156807804320835 1106 68-69 0.8117765634140497 1106 70-71 0.7387661797287848 1106 72-73 0.804182862901154 1106 74-75 0.657719919922453 1106 76-77 0.2902546197251503 1106 78-79 0.4151093587666743 1106 80-81 0.289142499202395 1106 82-83 0.3481746577255187 1106 84-85 0.5358547893125163 1106 86-87 0.33553097351016703 1106 88-89 0.711907951156352 1106 90-91 1.0238656739826517 1106 92-93 1.2428610878196658 1106 94-95 1.2292366345995482 1106 96-97 1.3566828126887565 1106 98-99 1.8528453286307496 1106 100-101 2.4766731215601894 1107 1 0.17152968868784768 1107 2 0.21474036861031465 1107 3 0.10822799233477554 1107 4 0.24747458669428113 1107 5 0.19820922257815 1107 6 0.23110985100995407 1107 7 0.255733707230938 1107 8 0.2429656072020947 1107 9 0.25121462078094936 1107 10-11 0.411347895551053 1107 12-13 0.25716678867502196 1107 14-15 0.21120760635210445 1107 16-17 0.5018425817537562 1107 18-19 0.40074440728065497 1107 20-21 0.09649856059535722 1107 22-23 0.2555850267142148 1107 24-25 0.28373177826021134 1107 26-27 0.3625316575273203 1107 28-29 0.20251447832513492 1107 30-31 0.22689816108714211 1107 32-33 0.2032874387566963 1107 34-35 0.33735648122920026 1107 36-37 0.017639451269225503 1107 38-39 0.005273183799893388 1107 40-41 0.04448254914409233 1107 42-43 0.06054729546534077 1107 44-45 0.15004574401120152 1107 46-47 0.02642728303683839 1107 48-49 0.4008769085378532 1107 50-51 0.165347142530905 1107 52-53 0.0680592123914181 1107 54-55 0.02285572255238577 1107 56-57 0.3129773645593872 1107 58-59 0.31660493455275684 1107 60-61 0.29021206233662156 1107 62-63 0.29800245225098365 1107 64-65 0.2762795949733814 1107 66-67 0.4052660326260238 1107 68-69 0.5437001009759754 1107 70-71 0.6182075060796706 1107 72-73 0.6239143285094961 1107 74-75 0.6240856057595252 1107 76-77 0.566946609486326 1107 78-79 0.6024640543024553 1107 80-81 0.43956050638836075 1107 82-83 0.3040314550390022 1107 84-85 0.5155356478710971 1107 86-87 0.6843174610756293 1107 88-89 0.5264554231522212 1107 90-91 0.6157010308151918 1107 92-93 0.7350968391758386 1107 94-95 0.8366826811439445 1107 96-97 0.9260238642821186 1107 98-99 0.7677350733645625 1107 100-101 0.3634853470962014 1108 1 -0.03953759282779146 1108 2 -0.2152582676775623 1108 3 -0.02370863040689386 1108 4 -0.07554840953277875 1108 5 -0.07491706221545513 1108 6 0.03106843456762931 1108 7 0.16494583517744132 1108 8 -0.0664253185564121 1108 9 0.17428105376460223 1108 10-11 0.09619907415187612 1108 12-13 0.09843069727600806 1108 14-15 0.031316299259287916 1108 16-17 0.1856886540102991 1108 18-19 0.048156637253029544 1108 20-21 0.05202639294967781 1108 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-1.9162791806551347 2316 90-91 -1.8264081662235334 2316 92-93 -1.9129516883217192 2316 94-95 -1.9458715207760058 2316 96-97 -1.5560910275174287 2316 98-99 -2.2838101389686614 2316 100-101 -2.9598143600303857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 3.0 5 33.0 6 76.0 7 136.0 8 199.0 9 372.0 10 646.0 11 953.0 12 1458.0 13 1938.0 14 2448.0 15 3468.0 16 4671.0 17 6269.0 18 5187.0 19 2828.0 20 2412.0 21 2571.0 22 2836.0 23 3173.0 24 3679.0 25 4453.0 26 5342.0 27 6484.0 28 7910.0 29 9716.0 30 11640.0 31 14463.0 32 17984.0 33 22632.0 34 30110.0 35 41039.0 36 54423.0 37 85490.0 38 64185.0 39 4119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.16465816236553 25.759332196813826 17.90794191993416 19.168067720886487 2 13.899560341381987 25.480920696870662 40.48080314109045 20.138715820656902 3 16.156956787511167 30.54732684440683 34.837776837353644 18.45793953072836 4 10.485556498576457 21.974058827263388 43.0627229062389 24.477661767921248 5 9.250416722503955 41.20556821200772 38.154618742990955 11.389396322497372 6 22.862093448627704 44.22352625862239 19.54996638031156 13.364413912438344 7 21.44677509603946 37.793702068433696 25.941468827730834 14.818054007796006 8 22.355159434532275 35.89351752804181 23.149099441162157 18.602223596263755 9 23.694301358655405 18.762798374033437 26.132310795656256 31.41058947165491 10-11 20.384650649939932 29.958480957900257 31.240493056257595 18.416375335902217 12-13 22.358452306082746 29.688192456667274 30.67845324650135 17.27490199074863 14-15 20.289904477990913 30.644509071416987 30.367206069397458 18.69838038119465 16-17 16.317618321041408 33.40872276047556 31.615245905108065 18.65841301337496 18-19 15.845650727523644 31.820842747215806 35.55062102236527 16.782885502895283 20-21 16.916741704851894 30.70315677612446 35.4956869696877 16.88441454933594 22-23 17.37463764879028 30.216035378173583 35.057964438446724 17.351362534589406 24-25 17.365289170704642 31.30059223627383 35.17434410831882 16.15977448470272 26-27 16.77320913436106 30.499672026313128 36.45057236698257 16.276546472343238 28-29 16.173092612141545 30.667147858266347 36.54313208709411 16.616627442497997 30-31 16.97898700752085 30.172860755564805 35.76652805357355 17.0816241833408 32-33 16.715489778885377 31.08335586406329 35.77131505013577 16.429839306915564 34-35 17.44358003183358 30.500050548153585 35.52488967571596 16.531479744296877 36-37 17.820352918080733 29.720611417467698 35.1877735314966 17.271262132954966 38-39 16.517139229792637 31.569802981144495 35.20607043776744 16.706987351295435 40-41 16.409299088711876 30.959697365822464 36.15258198301562 16.478421562450038 42-43 16.601150144120677 30.90295247544306 34.80490809661963 17.690989283816634 44-45 16.987054023268197 30.388426322663157 34.69270203329302 17.931817620775632 46-47 17.976990524196154 31.28672740930042 33.73261564395146 17.003666422551966 48-49 17.25277773859399 31.46426673813789 33.57325565539585 17.70969986787227 50-51 16.53790904837697 31.396718443999838 34.011759810225534 18.053612697397654 52-53 17.146336675330232 32.36212058695951 33.16417633236118 17.327366405349075 54-55 17.5012637097817 31.321076328070813 32.87361726639002 18.30404269575746 56-57 17.655999652047573 31.879260507046624 32.556709780637156 17.90803006026865 58-59 17.758747362419697 30.695850990672223 33.380628552284335 18.164773094623747 60-61 17.777866594881548 31.926666329684128 32.994744247884356 17.30072282754997 62-63 17.381808264781462 31.566227103988037 33.585519587536766 17.46644504369374 64-65 17.47046231719316 31.745882474641263 33.00744216773854 17.77621304042704 66-67 17.37360011190876 31.65971743037735 32.03458827097437 18.93209418673952 68-69 17.109559959280304 30.89289450730815 33.16421651028455 18.833329023127 70-71 17.930129741586544 31.384721361810573 33.107556092635285 17.577592803967597 72-73 17.926422426865592 30.173129233308334 33.68908209062248 18.211366249203593 74-75 17.062757364912443 32.16381074634796 32.31310149772714 18.460330391012462 76-77 17.04992417919571 31.385463564870868 33.00485488250714 18.55975737342628 78-79 17.16034202662767 31.16949220283674 32.80168897394363 18.86847679659196 80-81 17.513827417373466 31.3887370468986 32.00754703673009 19.089888498997844 82-83 17.34311834289617 31.31920125921459 32.54479604179671 18.792884356092532 84-85 16.77299374498194 31.16376721129171 32.20411507540465 19.8591239683217 86-87 16.69019672381669 31.598300193373575 32.41540640777726 19.296096675032473 88-89 17.315376124529646 30.835475714391507 32.21882497599005 19.630323185088795 90-91 16.983310097402825 30.30784277307704 32.847181242608976 19.861665886911155 92-93 17.684502300474673 31.119180339816666 32.48735738114998 18.70895997855868 94-95 17.26954698164087 30.10941654696048 32.599501113208746 20.0215353581899 96-97 17.535806312067407 30.155356605476978 32.109304468853445 20.199532613602166 98-99 17.150068240087553 30.638329887042374 31.55936534955265 20.65223652331742 100-101 16.738882554161915 30.39979310912318 32.07020183615653 20.79112250055837 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5820.0 1 3508.0 2 1116.0 3 964.5 4 730.5 5 670.5 6 1064.0 7 1897.0 8 2690.5 9 2807.5 10 2168.0 11 1527.5 12 1312.5 13 1213.0 14 1159.5 15 1029.5 16 952.5 17 993.0 18 1039.0 19 1103.0 20 1206.5 21 1359.5 22 1528.5 23 1735.5 24 1950.5 25 2577.5 26 4107.5 27 5723.0 28 6060.5 29 6431.0 30 8366.0 31 10240.5 32 12926.0 33 16769.0 34 20465.5 35 22951.0 36 25870.0 37 29383.0 38 28244.5 39 24752.5 40 22012.5 41 18904.5 42 15894.0 43 14637.5 44 14218.5 45 13373.5 46 11416.5 47 10818.5 48 14084.5 49 12303.5 50 6272.0 51 2965.5 52 1360.5 53 810.5 54 532.5 55 386.5 56 334.5 57 287.5 58 232.0 59 183.5 60 171.5 61 158.5 62 121.5 63 98.0 64 79.0 65 56.0 66 37.5 67 31.5 68 24.5 69 14.0 70 7.5 71 4.0 72 3.5 73 2.0 74 1.0 75 1.0 76 1.0 77 1.0 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016927355782455265 2 0.003996736781968604 3 0.0016457151455164841 4 0.0 5 9.404086545808481E-4 6 2.3510216364521202E-4 7 2.3510216364521202E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.001057959736403454 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0018808173091616961 22-23 0.0 24-25 0.00235102163645212 26-27 0.0018808173091616961 28-29 0.0032914302910329683 30-31 0.014693885227825752 32-33 4.7020432729042404E-4 34-35 0.00235102163645212 36-37 0.0017632662273390904 38-39 0.0016457151455164841 40-41 0.0035265324546781807 42-43 0.002115919472806908 44-45 5.8775540911303E-4 46-47 1.1755108182260601E-4 48-49 2.3510216364521202E-4 50-51 0.0 52-53 5.8775540911303E-4 54-55 0.0028212259637425442 56-57 3.5265324546781803E-4 58-59 0.001057959736403454 60-61 8.228575727582421E-4 62-63 0.0 64-65 1.1755108182260601E-4 66-67 1.1755108182260601E-4 68-69 0.0 70-71 3.5265324546781803E-4 72-73 0.0 74-75 5.8775540911303E-4 76-77 4.7020432729042404E-4 78-79 0.0 80-81 0.0034089813728555743 82-83 5.8775540911303E-4 84-85 0.001998368390984302 86-87 0.001057959736403454 88-89 5.8775540911303E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 7.053064909356361E-4 98-99 0.002468572718274726 100-101 4.7020432729042404E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 425347.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.706484352775497 #Duplication Level Percentage of deduplicated Percentage of total 1 69.19961760097723 12.252819462697515 2 10.06054651193669 3.562738187879543 3 4.211700347876889 2.2372321892478375 4 2.5214435563109117 1.7858360350490308 5 1.7048623097963196 1.5093558906022613 6 1.2175691106567172 1.2935321043759564 7 1.019730727354808 1.2639092317566598 8 0.8391534110523939 1.1886765393901921 9 0.665214966672863 1.060075655876261 >10 6.575138752423189 24.880156672081853 >50 1.1724247815811137 14.349225455921871 >100 0.7727646918235653 24.845126449698718 >500 0.0292110364606846 3.467756913766877 >1k 0.00929442069203601 5.026249156570988 >5k 0.0013277743845765727 1.277310055084437 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5433 1.277310055084437 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4740 1.114384255678305 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4683 1.1009834323505279 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4077 0.9585115211815295 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2727 0.6411236002604932 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2578 0.6060933778773566 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1396 0.32820262044871595 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1178 0.2769503487740598 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 944 0.22193644248108016 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 854 0.20077724775301106 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 850 0.19983683909843022 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 818 0.19231356986178344 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 812 0.19090295687991218 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 745 0.17515111191568297 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 699 0.1643364123880032 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 699 0.1643364123880032 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 696 0.16363110589706756 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 681 0.16010457344238938 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 672 0.15798865396958248 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 638 0.14999518040564527 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 635 0.14928987391470963 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 597 0.1403559916961916 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 592 0.13918048087796553 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 568 0.13353802895048045 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 568 0.13353802895048045 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 559 0.13142210947767352 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 543 0.12766047485935014 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 533 0.125309453222898 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 530 0.12460414673196238 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 517 0.12154781860457461 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 488 0.11472985585886347 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 484 0.11378944720428262 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 480 0.11284903854970178 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 478 0.11237883422241135 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 473 0.11120332340418529 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 472 0.11096822124054008 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 471 0.11073311907689486 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 456 0.10720658662221667 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 451 0.10603107580399063 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 443 0.10415025849482894 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 439 0.10320984984024807 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 436 0.10250454334931244 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 429 0.10085882820379595 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 428 0.10062372604015075 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.3510216364521202E-4 0.0 5 0.0 0.0 0.0 2.3510216364521202E-4 0.0 6 0.0 0.0 0.0 2.3510216364521202E-4 0.0 7 0.0 0.0 0.0 2.3510216364521202E-4 0.0 8 0.0 0.0 0.0 2.3510216364521202E-4 0.0 9 0.0 0.0 0.0 2.3510216364521202E-4 0.0 10-11 0.0 0.0 0.0 4.7020432729042404E-4 0.0 12-13 0.0 0.0 0.0 4.7020432729042404E-4 0.0 14-15 0.0 0.0 0.0 4.7020432729042404E-4 0.0 16-17 0.0 0.0 0.0 4.7020432729042404E-4 0.0 18-19 0.0 0.0 0.0 7.053064909356361E-4 0.0 20-21 0.0 0.0 0.0 0.001057959736403454 0.0 22-23 0.0 0.0 0.0 0.0018808173091616961 0.0 24-25 0.0 0.0 0.0 0.0038791857001459983 0.0 26-27 0.0 0.0 0.0 0.0063477584184207245 0.0 28-29 0.0 0.0 0.0 0.015399191718761389 0.0 30-31 0.0 0.0 0.0 0.03491267130131399 0.0 32-33 0.0 0.0 0.0 0.057364927929431736 0.0 34-35 0.0 0.0 0.0 0.08158045078488857 0.0 36-37 0.0 0.0 0.0 0.11061556799507226 0.0 38-39 0.0 0.0 0.0 0.13565394842328735 0.0 40-41 0.0 0.0 0.0 0.1581062050514051 0.0 42-43 0.0 0.0 0.0 0.18819928199799224 0.0 44-45 0.0 0.0 0.0 0.21417807108078815 0.0 46-47 0.0 0.0 0.0 0.26131605489165316 0.0 48-49 0.0 0.0 0.0 0.3182107784937945 0.0 50-51 0.0 0.0 0.0 0.3681699882684021 0.0 52-53 0.0 0.0 0.0 0.4470467641713707 0.0 54-55 0.0 0.0 0.0 0.587167653703917 0.0 56-57 0.0 0.0 0.0 0.7937049044662359 0.0 58-59 0.0 0.0 0.0 0.9875466383917131 0.0 60-61 0.0 0.0 0.0 1.1790373506807383 0.0 62-63 0.0 0.0 0.0 1.3894537871432031 0.0 64-65 0.0 0.0 0.0 1.6361935078888532 0.0 66-67 0.0 0.0 0.0 1.9497022431097433 0.0 68-69 0.0 0.0 0.0 2.3208110084237106 0.0 70-71 0.0 0.0 0.0 2.6881581391193543 0.0 72-73 0.0 0.0 0.0 3.04974526680569 0.0 74-75 0.0 0.0 0.0 3.479747124112783 0.0 76-77 0.0 0.0 0.0 3.9156265355110063 0.0 78-79 0.0 0.0 0.0 4.335871653026823 0.0 80-81 0.0 0.0 0.0 4.7989053643260675 0.0 82-83 0.0 0.0 0.0 5.262644382116248 0.0 84-85 0.0 0.0 0.0 5.830416107319436 0.0 86-87 0.0 0.0 0.0 6.471657258661751 0.0 88-89 0.0 0.0 0.0 7.132529440668442 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGACC 20 0.0020732062 71.24974 3 GGTATGC 55 4.958125E-5 43.181656 8 TATGCCA 25 0.0016156094 37.99986 10-11 CTTGAGG 25 0.0016156094 37.99986 66-67 TAGATAT 105 4.645881E-8 36.190342 7 TGGGGAC 55 0.0025778534 34.545326 8 CGCAGAA 85 1.6298378E-5 33.529285 9 CATTCCG 145 6.6211214E-10 32.758503 9 GTTATAT 60 0.0039414368 31.677721 1 TAGGCAT 165 7.6397555E-11 31.66655 5 CCTTTAG 140 1.6947524E-8 30.546375 1 CATATAG 160 1.899025E-9 29.687391 3 CTGTACA 80 4.4890313E-4 29.687391 9 GGCATTC 160 1.899025E-9 29.687391 7 CTTTAGG 155 4.573303E-8 27.580542 2 TAAGGTG 70 0.008385883 27.142757 5 ACATATA 175 4.942194E-9 27.142757 2 GACATAT 180 6.6484063E-9 26.398102 1 GAGTACT 3005 0.0 25.607225 12-13 TTAGGCA 205 9.531504E-10 25.48771 4 >>END_MODULE