##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936837_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 414011 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96259036595646 33.0 31.0 34.0 30.0 34.0 2 32.098910415423745 33.0 31.0 34.0 30.0 34.0 3 32.06165536664485 33.0 31.0 34.0 30.0 34.0 4 35.681242768911936 37.0 35.0 37.0 33.0 37.0 5 35.683921441700825 37.0 35.0 37.0 33.0 37.0 6 35.82247090053163 37.0 35.0 37.0 35.0 37.0 7 35.8084302107915 37.0 35.0 37.0 35.0 37.0 8 35.776184690744934 37.0 35.0 37.0 35.0 37.0 9 37.38882541768214 39.0 37.0 39.0 35.0 39.0 10-11 37.30106325677337 39.0 37.0 39.0 34.0 39.0 12-13 37.241142143566236 39.0 37.0 39.0 34.0 39.0 14-15 38.39357408378038 40.0 38.0 41.0 34.0 41.0 16-17 38.30739883722896 40.0 38.0 41.0 34.0 41.0 18-19 38.3137320022898 40.0 38.0 41.0 34.0 41.0 20-21 38.23367374296818 40.0 38.0 41.0 34.0 41.0 22-23 38.12723333438001 40.0 38.0 41.0 34.0 41.0 24-25 38.2035815473502 40.0 38.0 41.0 34.0 41.0 26-27 38.135900978476414 40.0 38.0 41.0 34.0 41.0 28-29 38.05621227455309 40.0 37.5 41.0 34.0 41.0 30-31 37.849214151314825 40.0 37.0 41.0 33.0 41.0 32-33 37.58168623538988 40.0 36.0 41.0 33.0 41.0 34-35 37.39962827074643 39.5 36.0 41.0 33.0 41.0 36-37 37.19150578124736 39.0 35.5 41.0 33.0 41.0 38-39 37.023562175884216 39.0 35.0 41.0 32.0 41.0 40-41 36.90284316117204 39.0 35.0 41.0 32.0 41.0 42-43 36.662505344060904 39.0 35.0 41.0 31.0 41.0 44-45 36.34445340824278 39.0 35.0 41.0 30.5 41.0 46-47 35.9103840236129 39.0 35.0 41.0 29.5 41.0 48-49 35.55852139194369 39.0 35.0 40.5 28.0 41.0 50-51 34.81062097383886 38.0 34.0 40.0 26.0 40.5 52-53 35.051358538782786 38.5 35.0 40.0 27.5 40.5 54-55 35.50908792278466 39.0 35.0 40.5 28.0 41.0 56-57 35.46724724705382 39.0 35.0 41.0 27.5 41.0 58-59 35.286282248539294 39.0 35.0 41.0 26.5 41.0 60-61 35.04295296501783 39.0 35.0 41.0 26.0 41.0 62-63 34.75699196398163 38.5 35.0 40.0 26.0 41.0 64-65 34.46763371021542 38.0 35.0 40.0 25.0 41.0 66-67 34.09056039573828 37.5 34.0 40.0 23.0 41.0 68-69 33.68768824982911 37.0 34.0 39.5 22.0 41.0 70-71 33.28700807466468 36.5 34.0 39.0 21.5 41.0 72-73 32.87015924697653 36.0 33.0 39.0 20.5 40.0 74-75 32.376762936250486 35.5 33.0 38.0 18.5 40.0 76-77 31.93552586767018 35.0 32.5 37.0 15.0 39.0 78-79 31.47320844132161 35.0 32.0 37.0 9.5 39.0 80-81 30.979277120656214 35.0 31.5 36.0 4.5 39.0 82-83 30.52037506249834 35.0 31.0 36.0 2.0 37.0 84-85 30.083156003101365 35.0 31.0 35.5 2.0 37.0 86-87 29.761700776066334 34.0 31.0 35.0 2.0 36.5 88-89 29.35371644714754 34.0 30.0 35.0 2.0 36.0 90-91 28.970203690240112 34.0 30.0 35.0 2.0 36.0 92-93 28.638443181461362 34.0 29.0 35.0 2.0 35.5 94-95 28.234883855742964 34.0 29.0 35.0 2.0 35.0 96-97 27.7551260715295 34.0 29.0 35.0 2.0 35.0 98-99 27.201133544760886 34.0 27.5 35.0 2.0 35.0 100-101 24.730468514121604 31.5 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3133056385679964 1101 2 0.342826369321827 1101 3 0.21180484292938928 1101 4 0.12931236174465965 1101 5 0.449477478747184 1101 6 0.12048830357948503 1101 7 0.14084703249263697 1101 8 0.2740310880823884 1101 9 0.3281121425473401 1101 10-11 0.42422800441753594 1101 12-13 0.5139369452808396 1101 14-15 0.6101322570696013 1101 16-17 0.5173040067979642 1101 18-19 0.4892901651059631 1101 20-21 0.40094861253626846 1101 22-23 0.24072502326777112 1101 24-25 0.283159325889649 1101 26-27 -0.010988752535773472 1101 28-29 0.07280267305463184 1101 30-31 0.24621385533577467 1101 32-33 0.24648685000527593 1101 34-35 0.1978025830509651 1101 36-37 0.2818017123452634 1101 38-39 0.4731466259172237 1101 40-41 0.3427334109855309 1101 42-43 0.47255868819833324 1101 44-45 0.3216001243418418 1101 46-47 0.48014431231051447 1101 48-49 0.7803614813274038 1101 50-51 0.9021338085934048 1101 52-53 1.3115472998239 1101 54-55 0.9274557849930929 1101 56-57 0.5951148174774943 1101 58-59 0.7334149657160083 1101 60-61 0.9582992615331776 1101 62-63 1.1090221760132408 1101 64-65 1.0855258303561683 1101 66-67 0.8645542580467804 1101 68-69 0.7076903604657829 1101 70-71 0.9206600798288775 1101 72-73 0.9433501084548723 1101 74-75 0.8259072410416977 1101 76-77 0.863097011709197 1101 78-79 0.8097145311342757 1101 80-81 0.8225680734881315 1101 82-83 0.8851955477965241 1101 84-85 0.6451197097302064 1101 86-87 0.8434252004839351 1101 88-89 1.0409344613647207 1101 90-91 1.0015256061636038 1101 92-93 1.0176273734270644 1101 94-95 1.3069443733565969 1101 96-97 1.1844043988568735 1101 98-99 1.3320479870016761 1101 100-101 1.7253627508600502 1102 1 0.31617419645394307 1102 2 0.3183173589419255 1102 3 0.36377365788474236 1102 4 0.3972991262315233 1102 5 0.5062075817692104 1102 6 0.3274712670652633 1102 7 0.38623500009023815 1102 8 0.5009774013703563 1102 9 0.47115151884644746 1102 10-11 0.500740148842624 1102 12-13 0.4495530376963188 1102 14-15 0.38046955544495376 1102 16-17 0.5805417862881015 1102 18-19 0.5310817511011905 1102 20-21 0.5009401946463115 1102 22-23 0.4879746396062501 1102 24-25 0.060784574379276535 1102 26-27 0.3493325518486543 1102 28-29 0.3331259200290546 1102 30-31 0.2555836143898702 1102 32-33 0.32555350026785135 1102 34-35 0.004113410400037765 1102 36-37 -0.1486179518456865 1102 38-39 0.13581587407013984 1102 40-41 -0.015331251359953058 1102 42-43 0.08831115043114579 1102 44-45 -0.15359623912969766 1102 46-47 -0.12249566748654672 1102 48-49 0.05396556853674639 1102 50-51 -0.19523851467947395 1102 52-53 0.10931396886484634 1102 54-55 0.06672977436064542 1102 56-57 -0.03171040770105549 1102 58-59 0.017813377972458966 1102 60-61 -0.09936004703428836 1102 62-63 0.06249714574121157 1102 64-65 0.07918651168723301 1102 66-67 0.19920099805700886 1102 68-69 0.01821660809419967 1102 70-71 -0.07843014052558317 1102 72-73 -0.2956499757240252 1102 74-75 -0.17344199131149196 1102 76-77 -0.2271136297987688 1102 78-79 -0.14458603933965009 1102 80-81 -0.019337477786528723 1102 82-83 0.07739216380476321 1102 84-85 -0.11914051962492067 1102 86-87 -0.0846633557184262 1102 88-89 -0.25009142040925525 1102 90-91 -0.34334563249652206 1102 92-93 -0.2410446720554411 1102 94-95 -0.12750292741461067 1102 96-97 -0.1739419390372312 1102 98-99 -0.49877832602727423 1102 100-101 -0.4571241528457648 1103 1 0.32066667155814343 1103 2 0.3634604941903845 1103 3 0.2482920895117644 1103 4 0.22952389973590925 1103 5 0.13393738434988478 1103 6 0.1760873351512231 1103 7 0.20642992359336887 1103 8 0.26303844911537766 1103 9 0.2708807224304408 1103 10-11 0.21838808854362668 1103 12-13 0.3424199855586565 1103 14-15 0.4079422886168871 1103 16-17 0.2506194062746303 1103 18-19 0.374650024243671 1103 20-21 0.6590035635034752 1103 22-23 0.7176056326928375 1103 24-25 0.426228705459728 1103 26-27 0.6473926761404698 1103 28-29 0.6566964394090959 1103 30-31 0.7493471854084106 1103 32-33 0.6694680683662853 1103 34-35 0.8974920012603462 1103 36-37 0.804404485571645 1103 38-39 1.0020680267144684 1103 40-41 0.9805693651074634 1103 42-43 0.7227359888268339 1103 44-45 0.9595766962036123 1103 46-47 0.6664016794573158 1103 48-49 0.4976006048509234 1103 50-51 0.6872271054068833 1103 52-53 0.734342046834243 1103 54-55 0.7456706733548302 1103 56-57 0.5653894500372303 1103 58-59 0.6017509076103096 1103 60-61 0.519871490140936 1103 62-63 0.48659303512649643 1103 64-65 0.6255713171381032 1103 66-67 0.639079826020172 1103 68-69 0.7521622491454352 1103 70-71 0.7617375785449738 1103 72-73 0.7024719201004714 1103 74-75 0.7621034700407421 1103 76-77 1.0159729501797692 1103 78-79 0.6816331684872381 1103 80-81 0.6704196789758896 1103 82-83 0.5038341235222354 1103 84-85 0.7769335562979087 1103 86-87 0.8983089157269006 1103 88-89 0.7927961179639702 1103 90-91 0.7096031782454801 1103 92-93 1.0985895655964875 1103 94-95 0.7426239850070822 1103 96-97 0.9178543022586005 1103 98-99 0.7661716327917034 1103 100-101 0.9810699969533232 1104 1 0.2917465396658514 1104 2 0.34825624432777147 1104 3 0.1808117817630972 1104 4 0.42864461781613983 1104 5 0.08720263439568043 1104 6 0.30122472295118286 1104 7 0.35272503258769206 1104 8 0.32603075539858395 1104 9 0.49782247000325697 1104 10-11 0.33354196287958615 1104 12-13 0.334462942144107 1104 14-15 0.4838490480966442 1104 16-17 0.41996131871280085 1104 18-19 0.5574101451449351 1104 20-21 0.5176315173411723 1104 22-23 0.18637280616782448 1104 24-25 0.4581599199678763 1104 26-27 0.6311236708281385 1104 28-29 0.4700093172255393 1104 30-31 0.4722130949156451 1104 32-33 0.4065879621197226 1104 34-35 0.5546178484851154 1104 36-37 0.2852503364600878 1104 38-39 0.3180714156135309 1104 40-41 0.5543351647044545 1104 42-43 0.4690952121278045 1104 44-45 0.17212707173026587 1104 46-47 0.03823031107483388 1104 48-49 -0.16868442903807335 1104 50-51 -0.1567690477347199 1104 52-53 -0.3772626426693293 1104 54-55 -0.3058268536647688 1104 56-57 -0.456168985573683 1104 58-59 -0.2664483962914943 1104 60-61 -0.4384885574868278 1104 62-63 -0.18648124587735282 1104 64-65 -0.10504555408373051 1104 66-67 -0.2342944183469271 1104 68-69 -0.04848805310772519 1104 70-71 -0.19315342713372274 1104 72-73 -0.2076764587234905 1104 74-75 -0.3177646376712957 1104 76-77 -0.52635394119228 1104 78-79 -0.24614664466535174 1104 80-81 -0.33883109955497304 1104 82-83 -0.3896101886228429 1104 84-85 -0.17739644919760522 1104 86-87 -0.10963667870431237 1104 88-89 0.03051135885058187 1104 90-91 -0.2019877724780912 1104 92-93 -0.37024630177849005 1104 94-95 -0.26263109834151876 1104 96-97 -0.17520303424991468 1104 98-99 -0.18057914573916278 1104 100-101 -0.1276266507843573 1105 1 0.05358391612587354 1105 2 0.10243414190189526 1105 3 -0.009821641417495641 1105 4 0.05321784333276014 1105 5 0.0707381240632472 1105 6 0.09760837074992423 1105 7 0.027904727107006977 1105 8 0.034004699693284124 1105 9 0.2156796128603986 1105 10-11 0.260967838890366 1105 12-13 0.14936635633997497 1105 14-15 0.24236207594929482 1105 16-17 0.24593840766159047 1105 18-19 0.1786028675169078 1105 20-21 0.23052459910217493 1105 22-23 0.09976004785696091 1105 24-25 0.15565542760489137 1105 26-27 0.02255448843820318 1105 28-29 0.041370503209371634 1105 30-31 -0.1522478215264016 1105 32-33 0.009609076225736146 1105 34-35 -0.32453758727409365 1105 36-37 -0.415232592560578 1105 38-39 -0.5431931845661637 1105 40-41 -0.6475628586558315 1105 42-43 -0.641619073586476 1105 44-45 -0.8124528923308247 1105 46-47 -0.8670819081524783 1105 48-49 -0.9254229735124611 1105 50-51 -0.8053988770249276 1105 52-53 -0.5161665061014915 1105 54-55 -0.48700385276629987 1105 56-57 -0.5868181320247174 1105 58-59 -0.523434020729951 1105 60-61 -0.5886120974688609 1105 62-63 -0.2882220365332344 1105 64-65 -0.33479622793817754 1105 66-67 -0.14871490352213357 1105 68-69 -0.15891033522812137 1105 70-71 -0.07536366972132669 1105 72-73 -0.3549136554889927 1105 74-75 -0.5599030024511684 1105 76-77 -0.5974343544896747 1105 78-79 -0.3011107057614879 1105 80-81 -0.5159649719718686 1105 82-83 -0.6728649056039764 1105 84-85 -0.5915361616863741 1105 86-87 -0.4979116113188695 1105 88-89 -0.41626537070916925 1105 90-91 -0.6654199377970542 1105 92-93 -0.9300396530812733 1105 94-95 -0.5609127695005434 1105 96-97 -0.4808858734233219 1105 98-99 -0.6833082562512907 1105 100-101 -0.5857353659685458 1106 1 0.07766816521012032 1106 2 0.0658269990447522 1106 3 0.2526096772638198 1106 4 0.3220136308785513 1106 5 0.21501468083980058 1106 6 0.06796093485249344 1106 7 0.24010710805939084 1106 8 0.23876660445518638 1106 9 0.14445708538787017 1106 10-11 0.20262305867058927 1106 12-13 0.23835445157806845 1106 14-15 0.3520003543375694 1106 16-17 0.25727082524399947 1106 18-19 0.1807319883960261 1106 20-21 0.32130985551243185 1106 22-23 0.28792396727087777 1106 24-25 0.30526623346569437 1106 26-27 0.14386859100230964 1106 28-29 0.3496809427698082 1106 30-31 0.31104201363843487 1106 32-33 0.2011498271414922 1106 34-35 0.27459244935594285 1106 36-37 0.3840348067068149 1106 38-39 0.29893135755837363 1106 40-41 0.42790646368849394 1106 42-43 0.5364029044354979 1106 44-45 0.4724944519915155 1106 46-47 0.469811406865837 1106 48-49 0.5677088946194004 1106 50-51 0.4385457200446723 1106 52-53 0.5273545561695698 1106 54-55 0.3487676673802227 1106 56-57 0.6093471610155703 1106 58-59 0.3672825543616227 1106 60-61 0.5011658329340705 1106 62-63 0.7222633114521031 1106 64-65 0.5776694314024766 1106 66-67 0.45293344812621683 1106 68-69 0.5013804156876347 1106 70-71 0.4425278137951594 1106 72-73 0.5334792016538685 1106 74-75 0.586330971907806 1106 76-77 0.3757913781110567 1106 78-79 0.7414259242751413 1106 80-81 0.5943601196032233 1106 82-83 0.7163177867037156 1106 84-85 0.8878022082769981 1106 86-87 0.9008177842855289 1106 88-89 1.0298083472395518 1106 90-91 1.1398799706278595 1106 92-93 0.781853661937042 1106 94-95 1.00045398874121 1106 96-97 1.1654854635730167 1106 98-99 1.0158332272651869 1106 100-101 1.0903749820168045 1107 1 -0.004051551854423963 1107 2 0.03371493007923476 1107 3 0.0024936295184616597 1107 4 -0.11591187752109278 1107 5 -0.05413872322739621 1107 6 -0.06503530741099439 1107 7 -0.3474647310210699 1107 8 -0.1796242001425128 1107 9 -0.05763730011168633 1107 10-11 -0.24789915618352865 1107 12-13 -0.2292461231345797 1107 14-15 -0.3054211752822269 1107 16-17 -0.0915410668868546 1107 18-19 -0.1169636349461527 1107 20-21 0.029087817492985835 1107 22-23 0.1447928885794525 1107 24-25 0.14728104336843728 1107 26-27 0.07370391372555929 1107 28-29 0.10696984639492513 1107 30-31 -0.04473550625546352 1107 32-33 0.03678477409109604 1107 34-35 0.14237538481128809 1107 36-37 -0.24335246119769494 1107 38-39 -0.2083409678173922 1107 40-41 -0.010452842235466164 1107 42-43 0.0808768213232085 1107 44-45 -0.02657440300406222 1107 46-47 -0.01318207235609492 1107 48-49 -0.28958553508882545 1107 50-51 -0.566154213642335 1107 52-53 -0.5641960627517406 1107 54-55 -0.5368396491538121 1107 56-57 -0.47996263120370486 1107 58-59 -0.494452083127328 1107 60-61 -0.29879272144258806 1107 62-63 -0.47208903160713334 1107 64-65 -0.36973054649318726 1107 66-67 -0.46755726805415065 1107 68-69 -0.4466771660983966 1107 70-71 -0.36050324279192836 1107 72-73 -0.5957593040932565 1107 74-75 -0.7300179449799025 1107 76-77 -0.8965312346210368 1107 78-79 -0.987563907731932 1107 80-81 -1.1690848406089778 1107 82-83 -0.8630947906614459 1107 84-85 -0.9137036493711008 1107 86-87 -1.2364584093484225 1107 88-89 -1.0745987040425007 1107 90-91 -1.0464954714587904 1107 92-93 -1.297691541422818 1107 94-95 -1.1712165461836328 1107 96-97 -0.6931600934561644 1107 98-99 -0.9796547258736261 1107 100-101 -1.0305219012723228 1108 1 -0.2672050766721519 1108 2 0.04953185231789092 1108 3 -0.18811896486154822 1108 4 -0.08043796662101244 1108 5 -0.04971226828330799 1108 6 -0.2660108918535329 1108 7 -0.30807291451397134 1108 8 -0.09306183798273793 1108 9 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-1.6716866989806292 2316 76-77 -1.9802834263217939 2316 78-79 -2.5136611496678647 2316 80-81 -2.635052946144583 2316 82-83 -1.9223806441027662 2316 84-85 -1.906709688724309 2316 86-87 -1.77751209558037 2316 88-89 -1.9471572189738993 2316 90-91 -1.702324699701812 2316 92-93 -2.0592567636543144 2316 94-95 -1.9521355297911036 2316 96-97 -1.8064104900496325 2316 98-99 -1.9176302901495994 2316 100-101 -1.6826885540234287 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 634.0 3 141.0 4 161.0 5 160.0 6 212.0 7 243.0 8 320.0 9 376.0 10 499.0 11 792.0 12 1237.0 13 1680.0 14 1946.0 15 2802.0 16 3878.0 17 5592.0 18 4574.0 19 2143.0 20 1940.0 21 2004.0 22 2147.0 23 2411.0 24 2851.0 25 3296.0 26 3785.0 27 4413.0 28 5421.0 29 6601.0 30 8140.0 31 9930.0 32 13018.0 33 17439.0 34 24707.0 35 38134.0 36 51683.0 37 83250.0 38 90180.0 39 15271.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.29846347120217 25.27082528024739 16.848908001546192 19.581803247004252 2 13.716320348620927 24.816051249595386 40.836622589824486 20.631005811959206 3 15.845504220153344 30.72450394476841 34.49727755419527 18.93271428088297 4 10.251950204261098 21.534023786573126 43.3724461323264 24.841579876839376 5 9.42066635572966 41.06205126594056 37.85400299176178 11.663279386568004 6 22.46043443593399 44.389059366467734 19.70836252929664 13.44214366830164 7 21.36033026308479 37.98581066881173 25.811828307643253 14.842030760460222 8 22.039627884499215 35.90310498973058 23.189078168418508 18.8681889573517 9 23.67340824693947 19.110217710117684 26.00199565594505 31.214378386997794 10-11 20.221654496808057 30.070724091488348 31.0549425329009 18.652678878802703 12-13 22.366426138882748 30.06718106511163 30.318702333880594 17.24769046212503 14-15 20.456642734976665 30.94145271478128 29.861144662717777 18.740759887524277 16-17 16.315957941526403 33.78991297205564 31.218934768297913 18.67519431812005 18-19 16.174307117371526 32.4295401235388 34.56948635009747 16.826666408992192 20-21 17.234261253350308 31.19050523668664 34.433373716193444 17.141859793769605 22-23 17.664969346789505 30.822902223746695 34.01105351388694 17.501074915576854 24-25 17.639165994367175 31.935473741672745 34.01795160409853 16.40740865986155 26-27 16.856456539145746 31.284230713955324 35.284853886556014 16.574458860342915 28-29 16.308939790291372 31.19192373002128 35.565201218348655 16.933935261338686 30-31 17.0919371707515 30.99265478453471 34.69895727408209 17.216450770631695 32-33 16.85855034727464 31.681277845073602 34.76719737107482 16.69297443657694 34-35 17.52890325245374 31.296066162573172 34.318332506059626 16.856698078913457 36-37 17.83781107265266 30.6633157090029 34.07312849175505 17.42574472658939 38-39 16.548392550904566 32.30996835825222 33.98323711987633 17.158401970966885 40-41 16.279780343735244 31.859216130991847 35.02592325556961 16.835080269703305 42-43 16.588226627102454 31.638931862839225 33.67354209615457 18.099299413903754 44-45 17.05620992704291 31.35681341415254 33.52173435214807 18.065242306656472 46-47 17.97681704109311 31.870167700858186 32.92448751361679 17.22852774443191 48-49 17.29064515349871 31.996328591096834 32.85282964179609 17.860196613608366 50-51 16.559704935376114 31.97898123479811 33.24114576665838 18.220168063167407 52-53 17.08946292295225 33.1562439992319 32.27208554393076 17.48220753388509 54-55 17.479000730671917 31.967077493493395 32.15173821414122 18.40218356169347 56-57 17.673869802504534 32.662173211001196 31.620892757291802 18.04306422920247 58-59 17.725545703333516 31.459250688998015 32.581937020388544 18.233266587279925 60-61 17.94114947415205 32.49448064966497 32.19810240629182 17.36626746989116 62-63 17.25146587279062 32.246302860489976 32.93856921153858 17.563662055180824 64-65 17.55800050724025 32.315672516032414 32.30347459571744 17.82285238100989 66-67 17.441176506112136 32.207168850995785 31.35481548850585 18.996839154386233 68-69 17.076605695181225 31.58269801238755 32.62083176314849 18.71986452928274 70-71 18.101069792612922 31.96597344363053 32.325953342739524 17.60700342101703 72-73 18.05570486254966 30.888308839604832 32.86862286341691 18.187363434428597 74-75 17.07962560107841 32.89796795939542 31.587523418051017 18.434883021475155 76-77 17.163072930949294 32.07700568868894 32.34712478995099 18.41279659041077 78-79 17.230558125714175 31.76192627416117 32.252095115463874 18.755420484660785 80-81 17.65176375068264 32.07795879507242 31.43373913211577 18.83653832212916 82-83 17.582590164805826 31.946984761320284 31.925118572729843 18.545306501144047 84-85 17.149666256785387 31.58940525340568 31.63663420632409 19.624294283484844 86-87 17.172407712274147 32.094375470301124 31.86983876597816 18.863378051446563 88-89 17.82072620718477 31.45118236347078 31.66593793105114 19.06215349829331 90-91 17.48444766563991 30.871413903484928 32.39065048015945 19.25348795071571 92-93 18.2145562303164 31.674967956577994 32.01237522394154 18.09810058916407 94-95 17.81292802118033 30.559655433136296 32.363655249547676 19.2637612961357 96-97 17.89027782642884 30.648841453766256 31.927512196653907 19.533368523150997 98-99 17.713229564933773 31.128587046466254 31.41331593793224 19.74486745066773 100-101 17.61158254885663 30.892515415692113 31.862036804360855 19.633865231090404 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4073.0 1 2481.5 2 888.0 3 819.5 4 668.5 5 675.0 6 1046.0 7 1818.5 8 2583.5 9 2675.0 10 2063.0 11 1506.5 12 1372.0 13 1334.5 14 1230.5 15 1102.0 16 1047.0 17 1012.5 18 998.5 19 1070.0 20 1179.5 21 1329.5 22 1554.0 23 1786.5 24 1944.0 25 2485.0 26 3894.5 27 5483.0 28 5783.0 29 6178.5 30 8107.0 31 9976.0 32 12628.0 33 16383.0 34 20250.0 35 22948.5 36 25990.0 37 29229.0 38 27712.0 39 24224.5 40 21436.5 41 18408.0 42 15541.0 43 14333.5 44 13934.5 45 12901.5 46 11020.5 47 10311.5 48 13390.0 49 11804.5 50 5818.0 51 2559.5 52 1197.0 53 785.0 54 529.0 55 420.5 56 351.5 57 292.5 58 269.5 59 240.5 60 199.5 61 177.0 62 159.0 63 119.0 64 88.0 65 56.5 66 39.5 67 40.5 68 34.0 69 23.0 70 10.5 71 4.0 72 4.0 73 4.0 74 2.5 75 1.5 76 1.5 77 0.5 78 1.0 79 2.0 80 1.5 81 0.5 82 0.5 83 1.5 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021980092316387728 2 0.008936960612157648 3 0.010869276420191734 4 0.01932315808034086 5 0.05024021100888624 6 0.03405706611660077 7 0.009178500088161907 8 0.03888785563668598 9 0.027052421312477204 10-11 0.01896084886633447 12-13 0.03127936214255177 14-15 0.013284671180234341 16-17 0.015337756726270559 18-19 0.01256005275222156 20-21 0.013646980394240733 22-23 0.006038486900106519 24-25 0.002173855284038347 26-27 1.2076973800213039E-4 28-29 0.0019323158080340862 30-31 0.0 32-33 3.6230921400639117E-4 34-35 1.2076973800213039E-4 36-37 0.0 38-39 2.4153947600426078E-4 40-41 1.2076973800213039E-4 42-43 1.2076973800213039E-4 44-45 1.2076973800213039E-4 46-47 0.0 48-49 2.4153947600426078E-4 50-51 0.0 52-53 3.6230921400639117E-4 54-55 0.0020530855460362163 56-57 3.6230921400639117E-4 58-59 9.661579040170431E-4 60-61 0.00314001318805539 62-63 0.0020530855460362163 64-65 0.0014492368560255647 66-67 0.0095408093021683 68-69 0.011352355372200255 70-71 0.023912408124421816 72-73 0.014854677774262037 74-75 0.0163039146302876 76-77 0.028863967382509164 78-79 0.017390842272306774 80-81 0.04275248725275415 82-83 0.0314001318805539 84-85 0.016907763320298252 86-87 0.011473125110202386 88-89 0.01256005275222156 90-91 0.020772394936366425 92-93 0.028863967382509164 94-95 0.010144657992178951 96-97 0.01582083567827908 98-99 0.026207033146462295 100-101 0.03550630297262633 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 414011.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.553371770315284 #Duplication Level Percentage of deduplicated Percentage of total 1 70.35527840339526 13.05327636222226 2 9.559579758634607 3.547248744598573 3 4.168565216825277 2.3202282064969286 4 2.318617942275396 1.7207272270543534 5 1.6481585148347286 1.5289448831069707 6 1.245882858370328 1.3869196712164653 7 0.979000950359965 1.2714638016864286 8 0.760287972088058 1.1284724318919062 9 0.6964966867587518 1.1630125769605155 >10 6.376524806998815 25.3628526778274 >50 1.1209040136435238 14.352758743125182 >100 0.7368544387017822 24.730502329648246 >500 0.02343353338627576 2.8158672112576717 >1k 0.010414903727233672 5.617725132907097 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4517 1.0910338131112458 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4397 1.0620490759907346 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3512 0.8482866397269637 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3423 0.8267896263625846 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2391 0.5775208871261874 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2284 0.5516761631937316 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1609 0.38863701689085556 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1125 0.27173191050479334 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 771 0.18622693599928503 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 766 0.18501923861926373 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 762 0.1840530807152467 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 741 0.17898075171915723 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 730 0.17632381748311035 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 705 0.17028533058300385 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 691 0.16690377791894417 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 674 0.16279760682687175 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 622 0.1502375540746502 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 622 0.1502375540746502 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 607 0.14661446193458627 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 599 0.1446821461265522 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 589 0.14226675136650957 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 585 0.14130059346249255 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 585 0.14130059346249255 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 541 0.13067285651830507 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 538 0.1299482380902923 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 530 0.12801592228225822 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 495 0.11956204062210907 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 489 0.1181128037660835 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 476 0.11497279057802812 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 468 0.11304047476999404 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 467 0.11279893529398978 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 460 0.11110815896195995 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 454 0.10965892210593438 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 447 0.10796814577390455 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 444 0.10724352734589176 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 444 0.10724352734589176 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 443 0.10700198786988752 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 437 0.10555275101386197 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 435 0.10506967206185343 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 432 0.10434505363384064 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 418 0.10096350096978099 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.4153947600426075E-4 0.0 16-17 0.0 0.0 0.0 2.4153947600426075E-4 0.0 18-19 0.0 0.0 0.0 2.4153947600426075E-4 0.0 20-21 0.0 0.0 0.0 3.623092140063911E-4 0.0 22-23 0.0 0.0 0.0 9.66157904017043E-4 0.0 24-25 0.0 0.0 0.0 0.002898473712051129 0.0 26-27 0.0 0.0 0.0 0.006159256638108649 0.0 28-29 0.0 0.0 0.0 0.01292236196622795 0.0 30-31 0.0 0.0 0.0 0.03007166476253046 0.0 32-33 0.0 0.0 0.0 0.04975713205687772 0.0 34-35 0.0 0.0 0.0 0.07161645463526331 0.0 36-37 0.0 0.0 0.0 0.09118115219160844 0.0 38-39 0.0 0.0 0.0 0.11400663267401108 0.0 40-41 0.0 0.0 0.0 0.1339336394443626 0.0 42-43 0.0 0.0 0.0 0.16315991604087815 0.0 44-45 0.0 0.0 0.0 0.19009156761535323 0.0 46-47 0.0 0.0 0.0 0.236225607532167 0.0 48-49 0.0 0.0 0.0 0.2847750422090234 0.0 50-51 0.0 0.0 0.0 0.3299429242218202 0.0 52-53 0.0 0.0 0.0 0.4074770960191879 0.0 54-55 0.0 0.0 0.0 0.5347683998734333 0.0 56-57 0.0 0.0 0.0 0.7418885005470869 0.0 58-59 0.0 0.0 0.0 0.9369316274205275 0.0 60-61 0.0 0.0 0.0 1.1159123791396848 0.0 62-63 0.0 0.0 0.0 1.3171147626512338 0.0 64-65 0.0 0.0 0.0 1.5470603438072903 0.0 66-67 0.0 0.0 0.0 1.8498300769786309 0.0 68-69 0.0 0.0 0.0 2.219747784479156 0.0 70-71 0.0 0.0 0.0 2.592563965691733 0.0 72-73 0.0 0.0 0.0 2.971418633804416 0.0 74-75 0.0 0.0 0.0 3.393146558907855 0.0 76-77 0.0 0.0 0.0 3.8222414380294243 0.0 78-79 0.0 0.0 0.0 4.243848593394862 0.0 80-81 0.0 0.0 0.0 4.699754354352904 0.0 82-83 0.0 0.0 0.0 5.153244720550903 0.0 84-85 0.0 0.0 0.0 5.713374765404784 0.0 86-87 0.0 0.0 0.0 6.370603679612378 0.0 88-89 0.0 0.0 0.0 7.044257278188261 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGATAC 25 0.0050162836 57.00783 7 CATGGAT 35 2.7823032E-4 54.299732 4 CATATAG 125 1.3460522E-10 38.00981 3 GGCATTC 150 0.0 38.00522 7 GGGATGC 75 6.9050493E-6 38.00522 7 CATTCCG 155 1.8189894E-12 36.779247 9 GGATGCC 80 1.0749316E-5 35.62989 8 GGTACTC 95 8.6645196E-7 35.009033 3 CTCCCGC 95 8.672723E-7 35.004807 7 ATGGGTT 55 0.002574465 34.554375 5 TAGGCAT 170 3.6379788E-12 33.53807 5 TTAGGCA 185 0.0 33.386997 4 CCTCATC 100 1.2939181E-6 33.258587 3 ACTCCCG 100 1.2939181E-6 33.258587 6 GTGGTAC 100 1.2939181E-6 33.258587 1 CCTTTAG 175 3.6379788E-12 32.579838 1 CTTTAGG 180 5.456968E-12 31.674845 2 ATACCTT 210 0.0 31.674843 6 TGGTACT 105 1.8939663E-6 31.674843 2 TACTCCC 115 3.848969E-6 28.92051 5 >>END_MODULE