##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936836_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 419312 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85434235128019 31.0 31.0 34.0 30.0 34.0 2 32.007886728736594 31.0 31.0 34.0 30.0 34.0 3 32.09410415156256 33.0 31.0 34.0 30.0 34.0 4 35.84129955737017 37.0 35.0 37.0 35.0 37.0 5 35.67081075666807 37.0 35.0 37.0 33.0 37.0 6 35.77328814820468 37.0 35.0 37.0 33.0 37.0 7 35.726327889495174 37.0 35.0 37.0 33.0 37.0 8 35.68478841530889 37.0 35.0 37.0 33.0 37.0 9 37.30319904987217 39.0 37.0 39.0 34.0 39.0 10-11 37.22586403441829 39.0 37.0 39.0 33.0 39.0 12-13 37.14651023581486 39.0 37.0 39.0 33.0 39.0 14-15 38.20174953256763 40.0 37.5 41.0 33.0 41.0 16-17 38.15336909031938 40.0 37.0 41.0 33.0 41.0 18-19 38.30429727744496 40.0 37.5 41.0 33.5 41.0 20-21 38.34514514251917 40.0 38.0 41.0 34.0 41.0 22-23 38.2203860132789 40.0 37.0 41.0 33.5 41.0 24-25 38.11105095966727 40.0 37.0 41.0 33.0 41.0 26-27 37.86078981760598 40.0 37.0 41.0 33.0 41.0 28-29 37.7226599763422 40.0 37.0 41.0 33.0 41.0 30-31 37.58570110085092 39.0 36.0 41.0 33.0 41.0 32-33 37.36132402602358 39.0 35.5 41.0 33.0 41.0 34-35 37.201730453695575 39.0 35.0 41.0 32.0 41.0 36-37 36.9808161941466 39.0 35.0 41.0 31.5 41.0 38-39 36.77336923341092 39.0 35.0 41.0 31.0 41.0 40-41 36.50326964169878 39.0 35.0 40.5 30.5 41.0 42-43 36.25204620902812 39.0 35.0 40.0 30.0 41.0 44-45 35.938803325447395 39.0 35.0 40.0 30.0 41.0 46-47 35.47511757354905 38.0 35.0 40.0 28.0 41.0 48-49 35.01512716068226 38.0 35.0 40.0 25.5 41.0 50-51 34.7666427385813 38.0 34.5 40.0 24.0 41.0 52-53 34.59327183576907 38.0 34.0 40.0 24.0 41.0 54-55 34.251839918724 38.0 34.0 40.0 23.0 41.0 56-57 34.08021234784599 38.0 33.5 40.0 23.0 41.0 58-59 33.80974310298775 38.0 33.0 40.0 22.0 41.0 60-61 33.455277931468686 37.5 33.0 40.0 21.5 41.0 62-63 33.10803411302324 37.0 32.5 39.5 20.0 40.5 64-65 32.81666277139695 36.5 32.0 39.0 19.0 40.0 66-67 32.47999580264815 36.0 31.5 39.0 17.5 40.0 68-69 31.976505561491205 35.5 31.0 38.5 13.5 40.0 70-71 31.550569742816805 35.0 31.0 38.0 10.0 40.0 72-73 31.102643139237607 35.0 30.5 37.5 8.0 39.0 74-75 30.559863538367612 34.5 30.0 37.0 7.0 39.0 76-77 29.08610891174114 32.5 28.5 35.5 2.0 37.5 78-79 29.75957520891365 34.0 29.0 36.0 2.0 38.0 80-81 29.548776328843438 34.0 29.0 35.5 2.0 37.0 82-83 29.14999689968329 34.0 29.0 35.0 2.0 37.0 84-85 28.64087242912199 34.0 29.0 35.0 2.0 36.0 86-87 28.25804770671958 34.0 28.5 35.0 2.0 36.0 88-89 27.816519679856526 33.0 27.0 35.0 2.0 35.5 90-91 27.299905559583316 33.0 26.0 35.0 2.0 35.0 92-93 26.77068268973938 32.5 25.5 34.5 2.0 35.0 94-95 26.139296037318275 32.0 25.0 34.0 2.0 35.0 96-97 25.275244925020033 32.0 21.5 34.0 2.0 35.0 98-99 24.385950080131263 31.5 10.0 34.0 2.0 35.0 100-101 21.12848547143893 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34424826355217064 1101 2 0.40220634707668523 1101 3 0.5081412820111453 1101 4 0.3666286555312368 1101 5 0.2768979370236053 1101 6 0.3769182360497396 1101 7 0.44604678390274444 1101 8 0.4485116371112312 1101 9 0.47880115154642056 1101 10-11 0.4814690032075646 1101 12-13 0.5767196824386147 1101 14-15 0.560160361870345 1101 16-17 0.7221134828927944 1101 18-19 0.6150891272379297 1101 20-21 0.4386207671700646 1101 22-23 0.6217777561439206 1101 24-25 0.3014068554639948 1101 26-27 0.6627469647828477 1101 28-29 0.6121155213332017 1101 30-31 0.7302595155159182 1101 32-33 0.6474053071511747 1101 34-35 0.5691863563091673 1101 36-37 0.6370189093237073 1101 38-39 0.8784862549012686 1101 40-41 0.8108032204781139 1101 42-43 0.6819664844275124 1101 44-45 0.10284067562032106 1101 46-47 0.20759462188274114 1101 48-49 0.15767028191514498 1101 50-51 0.44094003714710084 1101 52-53 0.7400087914457387 1101 54-55 0.8284866946199259 1101 56-57 1.0097301701067138 1101 58-59 0.6217590678582425 1101 60-61 0.6585273386488311 1101 62-63 0.9829580564561766 1101 64-65 1.183166885436357 1101 66-67 0.794837515719756 1101 68-69 0.7216221824857669 1101 70-71 0.6151710909059034 1101 72-73 0.5272349512585777 1101 74-75 0.38998727247396303 1101 76-77 0.7355551749135962 1101 78-79 0.828535544422973 1101 80-81 0.44650085998868505 1101 82-83 0.944691546844524 1101 84-85 0.6150917963300699 1101 86-87 0.6947607248511538 1101 88-89 0.5757650468705684 1101 90-91 0.47719430484562864 1101 92-93 0.5144207794254072 1101 94-95 0.11293231511381663 1101 96-97 0.696197296017079 1101 98-99 1.043502935654491 1101 100-101 1.8194195106043978 1102 1 -0.08674156955736478 1102 2 0.2692215014983219 1102 3 0.16889324824938967 1102 4 0.18644809731139844 1102 5 -0.009675293361965487 1102 6 0.20253448389167517 1102 7 0.2640918155543872 1102 8 0.22400679345115293 1102 9 0.4429952336488583 1102 10-11 0.2040106059315221 1102 12-13 0.2986318491372586 1102 14-15 0.4123957589545597 1102 16-17 0.31559672191063726 1102 18-19 0.3657305525208727 1102 20-21 0.2038681055548679 1102 22-23 0.5329733249143089 1102 24-25 0.3454523906644553 1102 26-27 0.49179214030558427 1102 28-29 0.5916078961653568 1102 30-31 0.550075959981676 1102 32-33 0.3494063142488173 1102 34-35 0.4737218924687667 1102 36-37 0.5030959043361705 1102 38-39 0.6435117920200071 1102 40-41 0.3672966024079045 1102 42-43 0.5432178032458594 1102 44-45 0.7344437592573811 1102 46-47 0.7288425837089463 1102 48-49 0.938275859317784 1102 50-51 1.0436939696236038 1102 52-53 0.8500611845015626 1102 54-55 0.980072873403735 1102 56-57 0.7471354549714775 1102 58-59 0.4823030204766994 1102 60-61 0.4716987370758403 1102 62-63 0.5409171251306475 1102 64-65 0.5772957459773096 1102 66-67 0.18820805571591137 1102 68-69 0.22078593176642158 1102 70-71 0.2690801883545859 1102 72-73 0.39171737500690185 1102 74-75 0.34132587308781126 1102 76-77 0.3142556592796062 1102 78-79 0.36746466393189436 1102 80-81 0.710843009422792 1102 82-83 0.9510788238443304 1102 84-85 0.8058199039456433 1102 86-87 0.5614821424609744 1102 88-89 0.6496668308721034 1102 90-91 0.7101905402187327 1102 92-93 0.9034422162180391 1102 94-95 0.39949955087057987 1102 96-97 0.9349423804214538 1102 98-99 1.0338096208645418 1102 100-101 0.8021549796241558 1103 1 0.21738948457369034 1103 2 0.36964885192566754 1103 3 0.13345877531492079 1103 4 0.16962117423447864 1103 5 0.18806331062663872 1103 6 0.26984217619936857 1103 7 0.36834751356008866 1103 8 0.43937360256796154 1103 9 0.42189480629843246 1103 10-11 0.38523389652981166 1103 12-13 0.5220559944363998 1103 14-15 0.6753498187836158 1103 16-17 0.5332338301727475 1103 18-19 0.49722984027016537 1103 20-21 0.5830080628198289 1103 22-23 0.6327774559684372 1103 24-25 0.6433067353938 1103 26-27 0.6215998326132777 1103 28-29 0.5533244203818768 1103 30-31 0.5769189086996178 1103 32-33 0.5965573113998133 1103 34-35 0.49918485543172864 1103 36-37 0.6749709043361705 1103 38-39 0.7195000398832576 1103 40-41 1.0449141237754276 1103 42-43 0.6277530596561078 1103 44-45 0.8132810099696357 1103 46-47 0.7723343928257549 1103 48-49 0.35276125817819093 1103 50-51 0.16726946819910182 1103 52-53 0.14675812182350256 1103 54-55 0.3956711640020245 1103 56-57 0.07779784813386925 1103 58-59 0.2259372797359589 1103 60-61 0.10747152909863189 1103 62-63 0.4148932647317807 1103 64-65 0.49307209925365925 1103 66-67 0.08186012124298259 1103 68-69 0.2576894217949075 1103 70-71 0.2409908008902022 1103 72-73 0.3191123180268427 1103 74-75 0.37725001838695604 1103 76-77 0.5193393487382956 1103 78-79 0.7178920143592435 1103 80-81 0.5282842487390305 1103 82-83 1.0387301771206872 1103 84-85 0.8948957586465021 1103 86-87 0.4392421139709448 1103 88-89 0.6463726713279421 1103 90-91 0.8023824917856857 1103 92-93 0.7824486264744515 1103 94-95 0.867804394175419 1103 96-97 1.4072091183131938 1103 98-99 1.427684264739188 1103 100-101 1.9829776291968066 1104 1 0.05828313051320677 1104 2 0.3202095043211841 1104 3 0.28269000195439986 1104 4 0.16274044028952517 1104 5 -0.06064124255039616 1104 6 0.14541557069478017 1104 7 0.1495231846440106 1104 8 0.07331430932976701 1104 9 0.19037085115061814 1104 10-11 0.0927389933697711 1104 12-13 0.3826892768013366 1104 14-15 0.45481592479788446 1104 16-17 0.4214431404003989 1104 18-19 0.2533672850543951 1104 20-21 0.1740185113417425 1104 22-23 0.05595321199969305 1104 24-25 -0.06689543925791241 1104 26-27 0.16403102527382885 1104 28-29 0.19757560964453802 1104 30-31 0.0672667680268404 1104 32-33 0.30889378778444865 1104 34-35 0.4556599658632621 1104 36-37 0.21654200525360068 1104 38-39 0.5323504476304493 1104 40-41 0.8972013307071336 1104 42-43 0.7947064235704815 1104 44-45 0.8266389780294432 1104 46-47 0.9739356500462861 1104 48-49 1.016709610199939 1104 50-51 0.9911842660244545 1104 52-53 0.8382294096250646 1104 54-55 0.8987611232273025 1104 56-57 1.0256508043010442 1104 58-59 0.5727560021280738 1104 60-61 0.7857865987554504 1104 62-63 0.9097751879393954 1104 64-65 1.0552641651727939 1104 66-67 0.7818367607264989 1104 68-69 0.5626503460218899 1104 70-71 0.7678341580087817 1104 72-73 0.7003028548939874 1104 74-75 0.7063466035041834 1104 76-77 0.9891299535633031 1104 78-79 0.8169618410666857 1104 80-81 1.1321005076584996 1104 82-83 0.8675506834067868 1104 84-85 1.1142510083916584 1104 86-87 0.9037766025879996 1104 88-89 1.0575708534550259 1104 90-91 1.1159443334438564 1104 92-93 1.1778996968108437 1104 94-95 1.1354734393674022 1104 96-97 1.6898713677185597 1104 98-99 1.0941704183239658 1104 100-101 2.4174513804710145 1105 1 -0.06786365847761999 1105 2 0.07250308230283764 1105 3 0.08773587351403478 1105 4 0.004483559555580996 1105 5 -0.10192564622011702 1105 6 -0.09770369536026635 1105 7 -0.10735754930094288 1105 8 0.011387703825185724 1105 9 -0.08256492866589582 1105 10-11 -0.1308848598412311 1105 12-13 0.0019553318472063097 1105 14-15 -0.13348682749568752 1105 16-17 -0.20241007060877791 1105 18-19 -0.05970610944101651 1105 20-21 -0.2021282776490807 1105 22-23 -0.1550559623122325 1105 24-25 -0.2813449805423218 1105 26-27 -0.2602809013316758 1105 28-29 -0.34256200503435963 1105 30-31 -0.12080662646857121 1105 32-33 -0.1681704323990303 1105 34-35 -0.07989049285233563 1105 36-37 -0.041485517682183115 1105 38-39 -0.11329175420441118 1105 40-41 -0.03169775186167101 1105 42-43 0.11580734100167689 1105 44-45 0.0502628312404525 1105 46-47 0.26177968674352314 1105 48-49 0.3756545643283715 1105 50-51 0.1941659174005963 1105 52-53 0.11231197843240182 1105 54-55 0.3723849764383118 1105 56-57 0.4407884189799418 1105 58-59 0.2803248094675226 1105 60-61 0.38555724095728294 1105 62-63 0.07720638059994656 1105 64-65 -0.05368078895564565 1105 66-67 0.3093596965063128 1105 68-69 0.12687052950812472 1105 70-71 0.1164580112197946 1105 72-73 0.038605607187562896 1105 74-75 -0.13195614878939566 1105 76-77 -0.24366821157431318 1105 78-79 -0.1669831130617574 1105 80-81 -0.23143160243324346 1105 82-83 -0.33543096796935856 1105 84-85 -0.7171710099569673 1105 86-87 -0.3932417460358515 1105 88-89 -0.042199788746810185 1105 90-91 0.3533296545447726 1105 92-93 0.2467070362603856 1105 94-95 0.5678128889086835 1105 96-97 0.30225668881947954 1105 98-99 0.477197941259746 1105 100-101 1.1640110134985377 1106 1 -0.2176348698055044 1106 2 0.015499417131074722 1106 3 -0.11770724802852328 1106 4 0.010812241231995756 1106 5 -0.023090305768917574 1106 6 0.013793789102592768 1106 7 0.13994479322187914 1106 8 -0.14533253657366174 1106 9 0.15476632546027247 1106 10-11 0.07711079203573945 1106 12-13 0.1313319111719906 1106 14-15 0.40235388550541984 1106 16-17 0.2165349973448798 1106 18-19 -0.10922292142453216 1106 20-21 0.18894099637373074 1106 22-23 -0.04999813910554707 1106 24-25 0.1391481102674348 1106 26-27 0.22954027926339649 1106 28-29 0.2767040500115101 1106 30-31 0.28072887561442883 1106 32-33 0.1162332373257371 1106 34-35 0.05894211579383324 1106 36-37 -0.06487090707822318 1106 38-39 0.052514583583288754 1106 40-41 0.2171725329289984 1106 42-43 0.22548362210441297 1106 44-45 -0.027846873496962132 1106 46-47 -0.18923278849948844 1106 48-49 -0.2638410761660879 1106 50-51 0.09246878351938648 1106 52-53 0.2209126113005695 1106 54-55 0.30071493295708507 1106 56-57 0.17168570311043396 1106 58-59 0.1454931569531297 1106 60-61 0.15795804228676502 1106 62-63 0.05553406061451227 1106 64-65 -0.07755847238497182 1106 66-67 0.44351978028166883 1106 68-69 0.4550058449177854 1106 70-71 0.658852210687094 1106 72-73 0.4160272385304715 1106 74-75 0.3985916919463719 1106 76-77 0.417272098485558 1106 78-79 0.3139597011527364 1106 80-81 0.24996366699102524 1106 82-83 0.3693092829014013 1106 84-85 0.10238473024837802 1106 86-87 0.24192569084807047 1106 88-89 0.38601918819220415 1106 90-91 -0.10653557640657851 1106 92-93 0.5286748464909934 1106 94-95 0.5852935955355889 1106 96-97 0.7173943034983772 1106 98-99 0.02876929828390118 1106 100-101 0.7016587017085243 1107 1 0.04326211979883965 1107 2 0.30929713330056785 1107 3 0.15385912170447114 1107 4 0.05211517903064333 1107 5 0.139818573083339 1107 6 0.16177862703870005 1107 7 0.218534031012787 1107 8 0.16421039979684338 1107 9 0.46516019398827524 1107 10-11 0.43554364872805706 1107 12-13 0.352666506027127 1107 14-15 0.4899731201425297 1107 16-17 0.503923944747747 1107 18-19 0.48395712510895805 1107 20-21 0.3655570236511636 1107 22-23 0.2865128010257223 1107 24-25 0.4545910412824199 1107 26-27 0.3817916791360645 1107 28-29 0.47454577663021524 1107 30-31 0.561706078963411 1107 32-33 0.33565769775739085 1107 34-35 0.9012548660898716 1107 36-37 0.8794822952234611 1107 38-39 0.6400386090279397 1107 40-41 0.9586081685396124 1107 42-43 0.846952699943877 1107 44-45 1.030090895394629 1107 46-47 1.3326028447309 1107 48-49 1.3448342664382409 1107 50-51 1.00455797073041 1107 52-53 1.0719846073017791 1107 54-55 1.2780402687182573 1107 56-57 1.0652329000923046 1107 58-59 1.2836744233543982 1107 60-61 1.1709124430341546 1107 62-63 1.2886775706204077 1107 64-65 1.3916116009856054 1107 66-67 1.4455608273447709 1107 68-69 1.5176148148138253 1107 70-71 1.3401781177034167 1107 72-73 1.4156434264734195 1107 74-75 1.5056722805772047 1107 76-77 1.4570308621941948 1107 78-79 1.7482177537677153 1107 80-81 1.6763907635271984 1107 82-83 1.5992900625456805 1107 84-85 1.2986227770317917 1107 86-87 1.3076140830623366 1107 88-89 1.565595626297302 1107 90-91 1.4953114819269047 1107 92-93 1.4999246456591067 1107 94-95 1.6786224064322823 1107 96-97 1.6682336320355482 1107 98-99 1.4199987464871242 1107 100-101 1.6415757506996016 1108 1 0.21917891445300697 1108 2 0.11334423269970273 1108 3 0.1558422542995217 1108 4 0.10220544389739672 1108 5 -0.010058828366290129 1108 6 0.17588150204206698 1108 7 0.1181810549666622 1108 8 0.16756232499825785 1108 9 0.18466009968170027 1108 10-11 0.1959860812988552 1108 12-13 0.12546579468608599 1108 14-15 0.062155374339170066 1108 16-17 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-1.9367927444168522 2316 88-89 -2.3715548885849067 2316 90-91 -1.9449650027676917 2316 92-93 -2.492796471564766 2316 94-95 -2.7815637975457115 2316 96-97 -2.876723343560446 2316 98-99 -3.446501465108309 2316 100-101 -4.28526583485548 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 3.0 5 18.0 6 98.0 7 125.0 8 216.0 9 379.0 10 646.0 11 1005.0 12 1381.0 13 1947.0 14 2565.0 15 3374.0 16 4594.0 17 6190.0 18 5156.0 19 2846.0 20 2530.0 21 2658.0 22 2886.0 23 3334.0 24 3987.0 25 4638.0 26 5399.0 27 6576.0 28 8196.0 29 10124.0 30 12309.0 31 14664.0 32 18505.0 33 23323.0 34 31144.0 35 41920.0 36 57327.0 37 86166.0 38 51211.0 39 1871.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.19120232225872 25.704894727928885 17.771862410464475 19.332040539347926 2 13.92879033238969 25.49910922329518 40.345387636925615 20.22671280738951 3 16.191084898798266 30.50718917462218 34.79183708512297 18.50988884145658 4 10.482647765864082 22.064477048116913 42.937478536268934 24.51539664975007 5 9.190728423121227 41.13421559111192 38.19123640899302 11.483819576773836 6 22.825962529095282 44.126807723127406 19.58040790628458 13.466821841492733 7 21.548973313300422 37.73651952016408 25.8059669456965 14.908540220838997 8 22.48945892318846 35.9510340748655 23.014604876559698 18.544902125386347 9 23.535219597817377 18.877351470981036 26.08153355973595 31.505895371465638 10-11 20.427986797420537 29.9581218758347 31.22698611058114 18.386905216163623 12-13 22.428351838888975 29.63126820792843 30.62080166758688 17.319578285595718 14-15 20.36968581118144 30.73953856647476 30.24919986072091 18.64157576162289 16-17 16.38588926622658 33.33782481779677 31.58638436295646 18.689901553020185 18-19 15.921156185161337 31.89060599556414 35.51560897665212 16.6726288426224 20-21 16.987353002201242 30.630871266774623 35.311429041589854 17.070346689434285 22-23 17.381780283517447 30.181801270677123 35.011232900234674 17.425185545570756 24-25 17.552133515220316 31.23408091426827 34.96510917988686 16.248676390624553 26-27 16.840364311930763 30.39617372308382 36.34199913667898 16.421462828306435 28-29 16.211913801656046 30.578304460640286 36.49275479833632 16.717026939367344 30-31 17.0022763506367 30.160036154505658 35.6526114833483 17.18507601150934 32-33 16.829155447865666 31.069301783163333 35.641087402906194 16.460455366064807 34-35 17.398500400656314 30.506520204525504 35.54823138856031 16.546748006257868 36-37 17.79295903802377 29.933091424230895 35.06696223618453 17.2069873015608 38-39 16.543457806282042 31.478422847084666 35.20557534503052 16.772544001602768 40-41 16.401011663222512 30.967817328250373 35.97521639743435 16.65595461109276 42-43 16.62210955088335 30.882850955851488 34.760154729652385 17.734884763612776 44-45 16.939416993195948 30.41372830060886 34.63841701465984 18.00843769153536 46-47 17.99489876833516 31.364466189136692 33.58835516878304 17.052279873745114 48-49 17.284524750692505 31.491504525871576 33.50134685073031 17.722623872705615 50-51 16.721359954973433 31.284640407528595 33.85601991853244 18.137979718965532 52-53 17.175696768862213 32.407726493851214 33.02099518137514 17.39558155591143 54-55 17.599544014957836 31.20909966802763 32.8770700576182 18.314286259396344 56-57 17.786636204067616 31.873402144465217 32.35407047735338 17.985891174113785 58-59 17.788467271309365 30.847151636851883 33.15963568806924 18.204745403769518 60-61 17.82194864848106 31.97795881766538 32.78095233552584 17.419140198327714 62-63 17.366662532911054 31.59031938031824 33.57201797229748 17.47100011447323 64-65 17.564009615751516 31.764175601938415 32.97067577364826 17.70113900866181 66-67 17.486859426870684 31.60343610485748 31.98227095814096 18.927433510130882 68-69 17.05271617878355 30.884795670998766 33.21969466613723 18.84279348408045 70-71 17.98261670051191 31.34646043922105 33.04520158689086 17.625721273376175 72-73 17.95349990818271 30.07135515166547 33.67202386772586 18.303121072425956 74-75 17.063058142848796 32.173950372462905 32.34196709543326 18.421024389255038 76-77 17.148447933758156 31.4304145838898 32.784179799290264 18.636957683061777 78-79 17.21047479022159 31.084408607920366 32.735806196586545 18.969310405271496 80-81 17.48776843558798 31.36026443789656 31.97998113542783 19.171985991087624 82-83 17.385622162017782 31.40167703285382 32.43801751440455 18.774683290723853 84-85 16.855967784825147 31.03012004373899 32.23389628802866 19.880015883407207 86-87 16.763213939057167 31.438478837903133 32.36428331238627 19.434023910653426 88-89 17.501579995468692 30.70736099020999 32.10670037323666 19.68435864108465 90-91 17.12685112203126 30.14477476499887 32.8119168197965 19.91645729317337 92-93 17.73667340786813 30.967513450604798 32.391870492616476 18.903942648910597 94-95 17.34126378448506 29.99115217308353 32.52351470981036 20.144069332621058 96-97 17.491888488629577 30.0535520728623 32.07579567934579 20.37876375916234 98-99 17.17736617613916 30.586045424572006 31.45687980625336 20.77970859303547 100-101 16.673463595885593 30.357113046049793 32.04883295532867 20.920590402735943 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5100.0 1 3201.0 2 1149.0 3 923.0 4 740.0 5 669.5 6 963.0 7 1747.0 8 2482.5 9 2624.0 10 2083.0 11 1455.0 12 1317.0 13 1248.5 14 1128.5 15 1026.5 16 948.5 17 970.0 18 992.5 19 1037.5 20 1180.5 21 1357.0 22 1566.5 23 1801.5 24 2012.5 25 2553.5 26 3962.5 27 5559.5 28 5844.5 29 6242.0 30 8153.0 31 10010.5 32 12621.0 33 16360.5 34 20256.0 35 22830.5 36 25699.5 37 29133.0 38 28235.5 39 24756.0 40 21588.0 41 18536.5 42 15761.5 43 14578.5 44 14048.5 45 13083.0 46 11326.5 47 10820.5 48 13944.0 49 12198.0 50 6206.0 51 2944.0 52 1403.0 53 779.0 54 490.5 55 374.5 56 319.5 57 286.5 58 241.5 59 191.0 60 174.0 61 148.5 62 124.0 63 104.5 64 75.0 65 59.5 66 40.5 67 31.0 68 17.5 69 8.5 70 5.5 71 4.0 72 2.5 73 0.5 74 1.0 75 0.5 76 1.5 77 1.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015501583546380737 2 0.0035772885107032467 3 2.384859007135498E-4 4 0.0 5 0.0021463731064219485 6 0.0 7 4.769718014270996E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.005604418666768421 14-15 0.002384859007135498 16-17 0.0 18-19 4.769718014270996E-4 20-21 7.154577021406494E-4 22-23 0.0019078872057083985 24-25 9.539436028541992E-4 26-27 2.384859007135498E-4 28-29 0.0 30-31 1.192429503567749E-4 32-33 2.384859007135498E-4 34-35 0.0 36-37 0.0020271301560651736 38-39 0.008943221276758118 40-41 5.962147517838745E-4 42-43 0.0 44-45 7.154577021406494E-4 46-47 3.577288510703247E-4 48-49 1.192429503567749E-4 50-51 9.539436028541992E-4 52-53 3.577288510703247E-4 54-55 0.0 56-57 0.0 58-59 2.384859007135498E-4 60-61 0.0014309154042812988 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.192429503567749E-4 70-71 3.577288510703247E-4 72-73 2.384859007135498E-4 74-75 0.0020271301560651736 76-77 0.0 78-79 1.192429503567749E-4 80-81 0.0017886442553516234 82-83 0.0 84-85 0.0015501583546380737 86-87 2.384859007135498E-4 88-89 0.0016694013049948489 90-91 0.0013116724539245241 92-93 0.0 94-95 0.0 96-97 1.192429503567749E-4 98-99 3.577288510703247E-4 100-101 0.001192429503567749 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 419312.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.29473041553783 #Duplication Level Percentage of deduplicated Percentage of total 1 69.7061737407446 12.752556568855649 2 9.950203358014392 3.640725760293052 3 4.17275002607154 2.2901801045522188 4 2.496349984357076 1.8268019994657916 5 1.6907393888830953 1.5465810661273707 6 1.2540410887475233 1.3765406189186096 7 0.9620398373135886 1.2320181630861984 8 0.8434143289185524 1.2344030220933337 9 0.6674314318489937 1.0989430304880377 >10 6.374491604964021 25.209867592627923 >50 1.1080404630305558 14.085454267943678 >100 0.7391281676921473 24.560947456786355 >500 0.024767963291271247 3.072890830694089 >1k 0.00912503910731046 4.868451177166406 >5k 0.0013035770153300658 1.2036383409012859 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5047 1.2036383409012859 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4490 1.0708016942038387 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4320 1.030259091082535 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3904 0.9310489563856985 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2646 0.6310336932880528 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2499 0.595976265883161 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1424 0.33960392261609496 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1131 0.26972755370702484 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 889 0.2120139657343458 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 876 0.20891364902506965 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 808 0.19269660777654823 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 784 0.18697294615942306 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 778 0.18554203075514175 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 733 0.174810165223032 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 725 0.1729022780173236 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 723 0.1724253062158965 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 658 0.1569237226695158 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 651 0.15525432136452094 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 631 0.15048460335024993 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 605 0.14428396993169762 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 604 0.14404548403098408 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 603 0.14380699813027054 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 586 0.13975273781814018 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 585 0.13951425191742664 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 564 0.1345060480024421 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 547 0.13045178769031174 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 535 0.12758995688174915 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 497 0.11852749265463425 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 496 0.1182890067539207 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 489 0.11661960544892586 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 484 0.1154271759453581 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 482 0.114950204143931 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 476 0.11351928873964971 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 472 0.1125653451367955 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 467 0.11137291563322775 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 467 0.11137291563322775 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 456 0.10874957072537872 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 448 0.10684168351967033 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 442 0.105410768115389 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 440 0.10493379631396194 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 434 0.10350288090968061 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 425 0.10135650780325868 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.192429503567749E-4 0.0 16-17 0.0 0.0 0.0 2.384859007135498E-4 0.0 18-19 0.0 0.0 0.0 4.769718014270996E-4 0.0 20-21 0.0 0.0 0.0 8.347006524974243E-4 0.0 22-23 0.0 0.0 0.0 0.002146373106421948 0.0 24-25 0.0 0.0 0.0 0.004531232113557446 0.0 26-27 0.0 0.0 0.0 0.008823978326401343 0.0 28-29 0.0 0.0 0.0 0.020986759262792386 0.0 30-31 0.0 0.0 0.0 0.04292746212843897 0.0 32-33 0.0 0.0 0.0 0.06379497844087458 0.0 34-35 0.0 0.0 0.0 0.08597416720723472 0.0 36-37 0.0 0.0 0.0 0.10839184187430839 0.0 38-39 0.0 0.0 0.0 0.12711298508032204 0.0 40-41 0.0 0.0 0.0 0.14511867058419506 0.0 42-43 0.0 0.0 0.0 0.17278303506696685 0.0 44-45 0.0 0.0 0.0 0.2000896706986683 0.0 46-47 0.0 0.0 0.0 0.24158621742282596 0.0 48-49 0.0 2.384859007135498E-4 0.0 0.2979881329415805 0.0 50-51 0.0 2.384859007135498E-4 0.0 0.3428234822757279 0.0 52-53 0.0 2.384859007135498E-4 0.0 0.4172310832983554 0.0 54-55 0.0 2.384859007135498E-4 0.0 0.5501869729461595 0.0 56-57 0.0 2.384859007135498E-4 0.0 0.7600545655740832 0.0 58-59 0.0 2.384859007135498E-4 0.0 0.9498893425420689 0.0 60-61 0.0 2.384859007135498E-4 0.0 1.1332850001907886 0.0 62-63 0.0 2.384859007135498E-4 0.0 1.3390983325065822 0.0 64-65 0.0 2.384859007135498E-4 0.0 1.5763918037165643 0.0 66-67 0.0 2.384859007135498E-4 0.0 1.892862593963445 0.0 68-69 0.0 2.384859007135498E-4 0.0 2.2690741023390695 0.0 70-71 0.0 2.384859007135498E-4 0.0 2.6337190445300873 0.0 72-73 0.0 2.384859007135498E-4 0.0 3.002537489983592 0.0 74-75 0.0 2.384859007135498E-4 0.0 3.4227496470408667 0.0 76-77 0.0 2.384859007135498E-4 0.0 3.84785076506277 0.0 78-79 0.0 2.384859007135498E-4 0.0 4.2606698591979235 0.0 80-81 0.0 2.384859007135498E-4 0.0 4.698529972908002 0.0 82-83 0.0 2.384859007135498E-4 0.0 5.134959171213798 0.0 84-85 0.0 2.384859007135498E-4 0.0 5.6889619185713745 0.0 86-87 0.0 2.384859007135498E-4 0.0 6.333470065249743 0.0 88-89 0.0 2.384859007135498E-4 0.0 7.021501888808334 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGAGT 50 2.8216356E-5 47.506443 6 ATGGGTC 45 9.6172973E-4 42.22291 5 GTCAGGC 45 9.6172973E-4 42.22291 2 ACTCCCG 90 5.680431E-7 36.94945 6 CATATAG 145 1.6370905E-11 36.03507 3 CCTTTAG 135 3.0559022E-10 35.202553 1 AGATCCT 55 0.0025775586 34.546017 5 CATTCCG 125 5.6206773E-9 34.208717 9 GTATGGT 70 2.0451771E-4 33.94532 1 GTGGTAC 100 1.2915934E-6 33.26641 1 TGGTACT 100 1.2964192E-6 33.25054 2 CTTTAGG 145 6.6211214E-10 32.759155 2 CTTGATA 60 0.003938587 31.682297 1 CTCCCGC 105 1.8958563E-6 31.670961 7 GGCATTC 135 1.1918019E-8 31.67096 7 TAGATAT 120 1.500157E-7 31.67096 7 GGTAGGG 75 3.0726928E-4 31.67096 7 TAGGCAT 150 9.513315E-10 31.667185 5 GGTACTC 105 1.8976225E-6 31.667183 3 CTGTACA 60 0.0039477996 31.667183 4 >>END_MODULE