FastQCFastQC Report
Thu 26 May 2016
SRR936835_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936835_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747232
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80911.0827962399897222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55740.7459530641086035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37400.5005138966211297No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23690.3170367436084108No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12100.1619309665538949No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10750.14386428846730334No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9900.13248897263500492No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9140.1223181020084793No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77450.036.099981
GGTATCA48900.035.200661
TAGTCGA753.0644872E-431.689415
TCGTTAC300.00396339431.64694844-45
TATCAAC97150.028.8305362
ATCAACG97250.028.740423
TCAACGC98300.028.4353354
CAACGCA99050.028.122155
AACGCAG100950.027.6399426
GCGTCCT700.00836038627.162359
TATCGCG458.94283E-426.39190790-91
CGGGTCG1109.426133E-525.9276966
GTACATG60000.025.3600331
GAGTACT50150.025.30135512-13
ACGCAGA110450.025.0904377
CGCAGAG109500.024.9608358
TACATGG60450.024.8582742
AGTACTT51450.024.47732412-13
GCAGAGT111800.023.9371249
ACATGGG59900.023.6480223
Produced by FastQC (version 0.11.5)