##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936835_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 747232 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.870915592479978 33.0 31.0 34.0 30.0 34.0 2 31.93116997130744 33.0 31.0 34.0 30.0 34.0 3 31.923416020727164 33.0 31.0 34.0 30.0 34.0 4 35.51474776240846 37.0 35.0 37.0 33.0 37.0 5 35.55068305425892 37.0 35.0 37.0 33.0 37.0 6 35.64365016487517 37.0 35.0 37.0 33.0 37.0 7 35.59895855637874 37.0 35.0 37.0 33.0 37.0 8 35.57474920774271 37.0 35.0 37.0 33.0 37.0 9 37.18462806732046 39.0 37.0 39.0 34.0 39.0 10-11 37.067529227870324 39.0 37.0 39.0 33.0 39.0 12-13 36.9654029806004 39.0 37.0 39.0 33.0 39.0 14-15 38.06196000706608 40.0 38.0 41.0 33.0 41.0 16-17 38.031255353089804 40.0 38.0 41.0 33.0 41.0 18-19 38.066330135754356 40.0 38.0 41.0 33.0 41.0 20-21 38.05724326581303 40.0 38.0 41.0 33.0 41.0 22-23 37.92870018949938 40.0 38.0 41.0 33.0 41.0 24-25 37.75952247762409 40.0 37.0 41.0 33.0 41.0 26-27 37.695029789944755 40.0 37.0 41.0 32.5 41.0 28-29 37.72336101772943 40.0 37.0 41.0 33.0 41.0 30-31 37.50746019977731 40.0 37.0 41.0 33.0 41.0 32-33 37.24206002954906 39.5 36.0 41.0 32.0 41.0 34-35 37.02325382210612 39.0 35.5 41.0 31.5 41.0 36-37 36.846468700483925 39.0 35.0 41.0 31.0 41.0 38-39 36.6620969123378 39.0 35.0 41.0 31.0 41.0 40-41 36.4057053231125 39.0 35.0 41.0 30.0 41.0 42-43 36.11751370390989 39.0 35.0 41.0 30.0 41.0 44-45 35.74630770630809 39.0 35.0 40.5 28.5 41.0 46-47 35.2141162316389 38.5 35.0 40.0 26.5 41.0 48-49 34.72828987516594 38.0 35.0 40.0 24.0 41.0 50-51 34.011640962913795 37.5 33.5 39.5 23.0 40.5 52-53 34.25797476553467 38.0 34.5 40.0 23.0 40.5 54-55 34.54230279217164 39.0 35.0 40.5 23.0 41.0 56-57 34.41830716564601 38.5 35.0 41.0 22.0 41.0 58-59 34.180770496980855 38.0 34.0 40.0 20.5 41.0 60-61 33.88934159671963 38.0 34.0 40.0 19.5 41.0 62-63 33.601454434499594 37.5 34.0 40.0 18.0 41.0 64-65 33.28752984347565 37.0 33.5 40.0 15.5 41.0 66-67 32.90906438696416 36.5 33.0 39.5 11.5 41.0 68-69 32.47324659543489 36.0 33.0 39.0 8.0 41.0 70-71 32.1259341141707 35.5 32.0 39.0 7.0 40.5 72-73 31.763606885144107 35.0 32.0 38.5 2.0 40.0 74-75 31.34960025801893 35.0 31.5 37.5 2.0 40.0 76-77 30.91551017622372 35.0 31.0 37.0 2.0 39.0 78-79 30.483982217035674 35.0 31.0 36.5 2.0 39.0 80-81 30.076364502590895 35.0 30.5 36.0 2.0 38.5 82-83 29.662417428589784 34.5 30.0 35.5 2.0 37.0 84-85 29.316778457025393 34.0 30.0 35.0 2.0 37.0 86-87 28.98661459894651 34.0 29.0 35.0 2.0 36.5 88-89 28.604169789302386 34.0 29.0 35.0 2.0 36.0 90-91 28.257859674103894 34.0 29.0 35.0 2.0 36.0 92-93 27.90058776926042 34.0 29.0 35.0 2.0 35.0 94-95 27.517274420795683 34.0 27.0 35.0 2.0 35.0 96-97 27.093296994775386 34.0 26.5 35.0 2.0 35.0 98-99 26.544165667423236 34.0 25.0 35.0 2.0 35.0 100-101 24.617956002954905 31.5 19.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37346826247334874 1101 2 0.40498576457664726 1101 3 0.47432422617735526 1101 4 0.366682675272493 1101 5 0.2958625231241001 1101 6 0.19187153598788598 1101 7 0.3559667073898112 1101 8 0.2985266968299882 1101 9 0.45791504114776416 1101 10-11 0.483041843205001 1101 12-13 0.5326937702577297 1101 14-15 0.7719369635514752 1101 16-17 0.7663977075929793 1101 18-19 0.7955563658285527 1101 20-21 0.6514819549334945 1101 22-23 0.7416466388910337 1101 24-25 0.9030864727899726 1101 26-27 0.9801944545789567 1101 28-29 0.8694734480386259 1101 30-31 0.8418388074018779 1101 32-33 0.8285967732058452 1101 34-35 0.855315188468758 1101 36-37 0.7849467706367577 1101 38-39 0.9184459589505423 1101 40-41 0.897993942814395 1101 42-43 0.872180416453638 1101 44-45 0.9086529518320958 1101 46-47 0.9081135982804085 1101 48-49 1.0113789850726889 1101 50-51 0.707387706036684 1101 52-53 0.6318266458921826 1101 54-55 0.5727624232015884 1101 56-57 0.676219476585679 1101 58-59 0.7282802854909391 1101 60-61 0.9034940035049388 1101 62-63 0.9798376962512378 1101 64-65 0.8904363084401723 1101 66-67 0.8692637640466359 1101 68-69 0.8526967160934404 1101 70-71 0.8714589368578842 1101 72-73 0.8777732948540233 1101 74-75 0.8473701529077395 1101 76-77 1.0559826230222207 1101 78-79 1.1372077713965432 1101 80-81 1.0957424976326564 1101 82-83 1.02757492437674 1101 84-85 1.1829905075506737 1101 86-87 1.1284732195156373 1101 88-89 1.0349041668439725 1101 90-91 0.8662153332331606 1101 92-93 0.8313737124035079 1101 94-95 1.108817323628685 1101 96-97 1.1679215180013287 1101 98-99 1.2180441730534852 1101 100-101 1.2244888529814801 1102 1 0.4914228923226709 1102 2 0.5299419935566299 1102 3 0.39650927549638126 1102 4 0.45475481873383217 1102 5 0.4153298441436277 1102 6 0.2723167170301224 1102 7 0.3448539400117241 1102 8 0.34230124498516545 1102 9 0.40108636510976226 1102 10-11 0.5404028277684958 1102 12-13 0.4513475897808803 1102 14-15 0.5481018901087751 1102 16-17 0.5689059524213818 1102 18-19 0.5020973659335155 1102 20-21 0.43299863290491913 1102 22-23 0.46983147122928415 1102 24-25 0.4902404171211785 1102 26-27 0.2954450276816374 1102 28-29 0.47150645204924047 1102 30-31 0.43212971158546054 1102 32-33 0.6214859780595106 1102 34-35 0.47070882775673795 1102 36-37 0.6423021983755532 1102 38-39 0.7158185511034247 1102 40-41 0.5752191644479154 1102 42-43 0.7059656460607471 1102 44-45 0.7014601273686623 1102 46-47 0.533248439483117 1102 48-49 0.7313070815250526 1102 50-51 0.48505209322917153 1102 52-53 0.6322588433693852 1102 54-55 0.8199022020399696 1102 56-57 0.829393790648588 1102 58-59 0.6390010762182641 1102 60-61 0.6942081084019094 1102 62-63 0.7404024989707239 1102 64-65 1.005047582090853 1102 66-67 0.8921115749664068 1102 68-69 0.8437008343179713 1102 70-71 0.9424513416487557 1102 72-73 0.9905137708533829 1102 74-75 0.9798408847591951 1102 76-77 1.029409534459667 1102 78-79 1.0843235588647566 1102 80-81 0.9327581828729379 1102 82-83 1.0340274563042264 1102 84-85 1.2101395654042726 1102 86-87 1.068578587496507 1102 88-89 1.0610582884643414 1102 90-91 0.9588683289350008 1102 92-93 1.0331980267751355 1102 94-95 0.9179919940340042 1102 96-97 0.7243594522385131 1102 98-99 0.7762638193395404 1102 100-101 1.29054891431824 1103 1 0.3739747928106105 1103 2 0.503693111168527 1103 3 0.3973866725587136 1103 4 0.48787055898674225 1103 5 0.4085725305018215 1103 6 0.19958561262613017 1103 7 0.3201762347649719 1103 8 0.17295422244930592 1103 9 0.42295243529944315 1103 10-11 0.5503833497623631 1103 12-13 0.4012316293962499 1103 14-15 0.49086868506389436 1103 16-17 0.6379652387981523 1103 18-19 0.7241581118566458 1103 20-21 0.6530270726717688 1103 22-23 0.5548195309089792 1103 24-25 0.8175058698987456 1103 26-27 0.6993424024804114 1103 28-29 0.8194841092008645 1103 30-31 0.8135752154210749 1103 32-33 0.8836273902618998 1103 34-35 0.6711163322663154 1103 36-37 0.6956512782562427 1103 38-39 0.9617938441863885 1103 40-41 0.9302452098910265 1103 42-43 1.1386554157779258 1103 44-45 1.2385778832352727 1103 46-47 1.1992063724149205 1103 48-49 1.2523197500536227 1103 50-51 1.2831485276168877 1103 52-53 1.1222245925423309 1103 54-55 1.1866416547570253 1103 56-57 1.263513373295531 1103 58-59 1.4053536813363863 1103 60-61 1.5140412464683237 1103 62-63 1.6070809199063945 1103 64-65 1.7122447418699664 1103 66-67 1.4869184485012568 1103 68-69 1.7975900193819783 1103 70-71 1.4799655432748082 1103 72-73 1.5449222998622645 1103 74-75 1.5542662731456325 1103 76-77 1.2719722641765152 1103 78-79 1.2226617417034547 1103 80-81 1.2734365432984696 1103 82-83 1.2823636882124774 1103 84-85 1.3265548193851622 1103 86-87 1.1085163246467076 1103 88-89 1.3804089103103827 1103 90-91 1.39829141689275 1103 92-93 1.3675410796370038 1103 94-95 1.1734830286218099 1103 96-97 1.0768957139845199 1103 98-99 1.1432959116237669 1103 100-101 1.5356301178858338 1104 1 0.2557689400279948 1104 2 0.3340716429666024 1104 3 0.5009984950747857 1104 4 0.4087398016059467 1104 5 0.3347528306296681 1104 6 0.4544446579045811 1104 7 0.4959596881522046 1104 8 0.4960074668007053 1104 9 0.37907104661857005 1104 10-11 0.5784329325043913 1104 12-13 0.4771421013232171 1104 14-15 0.6714336072598783 1104 16-17 0.6288760348223761 1104 18-19 0.5749616661691377 1104 20-21 0.5176337220440459 1104 22-23 0.7460837998076428 1104 24-25 0.7455990333775375 1104 26-27 0.6018470536207516 1104 28-29 0.7391383319122227 1104 30-31 0.6446005625843938 1104 32-33 0.7645071694896188 1104 34-35 0.7781449672928176 1104 36-37 0.8202639130841405 1104 38-39 0.6925145399335406 1104 40-41 0.6300794375948584 1104 42-43 0.879644477840479 1104 44-45 1.0996187732752887 1104 46-47 0.7762049836237281 1104 48-49 1.0458222207779855 1104 50-51 0.8589886635446504 1104 52-53 1.0423097956409677 1104 54-55 0.9198503140839307 1104 56-57 1.1507764892354615 1104 58-59 0.9072113791977614 1104 60-61 0.8905608833139027 1104 62-63 0.876173351063315 1104 64-65 0.8696904969545045 1104 66-67 0.718048748008485 1104 68-69 1.061417594127775 1104 70-71 0.8486289248365679 1104 72-73 0.7050091027601013 1104 74-75 0.603239436514091 1104 76-77 0.5478224678061245 1104 78-79 0.7480744140438951 1104 80-81 0.8937662674296476 1104 82-83 0.7071041690624646 1104 84-85 0.8459213540683947 1104 86-87 0.7724964319711702 1104 88-89 0.798790189349841 1104 90-91 0.8563884005816966 1104 92-93 0.7774165297529194 1104 94-95 0.7390113554111366 1104 96-97 0.7326092726964646 1104 98-99 0.7130747393846981 1104 100-101 1.0604835500757268 1105 1 0.11038543406234425 1105 2 0.20069810160040547 1105 3 0.09851608608916607 1105 4 0.026966277721498955 1105 5 -0.004300532422647052 1105 6 0.08345038317882825 1105 7 0.07415469711160227 1105 8 -0.037969195058479954 1105 9 0.01394714469120828 1105 10-11 0.11635085567208847 1105 12-13 0.13223759540127844 1105 14-15 0.12696789334876968 1105 16-17 0.33156472849834273 1105 18-19 0.3781538343435997 1105 20-21 0.47897806888203576 1105 22-23 0.4595097864340971 1105 24-25 0.4703706779734844 1105 26-27 0.29483075188568364 1105 28-29 0.3793227644108015 1105 30-31 0.18697283652347352 1105 32-33 0.21041512805786056 1105 34-35 0.2707921418044208 1105 36-37 0.013168069371026547 1105 38-39 0.17865850217295076 1105 40-41 0.22774999395166162 1105 42-43 0.1317401030316887 1105 44-45 0.01664774928157442 1105 46-47 -0.01490641884994659 1105 48-49 -0.008483972133156215 1105 50-51 -0.017514111683709643 1105 52-53 -0.012462285998815048 1105 54-55 -0.15530724021876807 1105 56-57 0.0196493531837163 1105 58-59 0.18834944134539455 1105 60-61 -0.022124751055223157 1105 62-63 0.029944602319815772 1105 64-65 0.20624571744311737 1105 66-67 0.055832935762524016 1105 68-69 0.42935693244340456 1105 70-71 0.4820645264274077 1105 72-73 0.41933977012604373 1105 74-75 0.3771959943568426 1105 76-77 0.250054887717841 1105 78-79 0.18500461814336333 1105 80-81 0.01225234758349103 1105 82-83 0.07488704568499216 1105 84-85 0.18394853819496504 1105 86-87 0.21856172632019266 1105 88-89 0.02590438890405622 1105 90-91 0.04043542649839438 1105 92-93 0.07704766475576008 1105 94-95 0.024423706269928402 1105 96-97 -0.04734352656930341 1105 98-99 0.14944727555007375 1105 100-101 0.40160587864528097 1106 1 0.57505804157881 1106 2 0.5557232856376011 1106 3 0.637686950095361 1106 4 0.35992249624145245 1106 5 0.29514929322633066 1106 6 0.3488978841474477 1106 7 0.5609117680143498 1106 8 0.3143438618961909 1106 9 0.49965036049903233 1106 10-11 0.6391327231653108 1106 12-13 0.6562437945488924 1106 14-15 0.6293778119849591 1106 16-17 0.6664659295831967 1106 18-19 0.7180840939329087 1106 20-21 0.6701365345930057 1106 22-23 0.8130936686502963 1106 24-25 0.7855585896356345 1106 26-27 0.7882984000840594 1106 28-29 0.6849949844805465 1106 30-31 0.8016105738345871 1106 32-33 0.6790241943112534 1106 34-35 0.6356662216184432 1106 36-37 0.4932378097817107 1106 38-39 0.6548847710609778 1106 40-41 0.5241467393991712 1106 42-43 0.4032627686651651 1106 44-45 0.32318010108320294 1106 46-47 0.3328347667370366 1106 48-49 0.14652571403498627 1106 50-51 -0.24282988076545564 1106 52-53 -0.10671257658385969 1106 54-55 0.14259279852590367 1106 56-57 0.04181905484974635 1106 58-59 0.06296393185294846 1106 60-61 0.13326463290448487 1106 62-63 0.1634819909288865 1106 64-65 -0.22185191913185065 1106 66-67 -0.2663832595106328 1106 68-69 -0.07200688003237588 1106 70-71 -0.08273206404140154 1106 72-73 -0.035367707595771236 1106 74-75 -0.12299772900619743 1106 76-77 -0.024482113444502573 1106 78-79 0.0740011311228308 1106 80-81 0.21028411821502857 1106 82-83 0.3202028147667413 1106 84-85 0.5442352487722566 1106 86-87 0.5709755194236408 1106 88-89 0.8334208553899138 1106 90-91 0.7623339154561641 1106 92-93 0.849748168436502 1106 94-95 0.7482051863164223 1106 96-97 0.309146206092457 1106 98-99 0.3467583952711166 1106 100-101 0.1573904582655814 1107 1 0.11276091298954682 1107 2 0.390826727763514 1107 3 0.361460804206299 1107 4 0.5331211764626076 1107 5 0.3975659153058757 1107 6 0.4572133826314868 1107 7 0.3394475268871915 1107 8 0.31678723624968086 1107 9 0.4745656449648834 1107 10-11 0.42716776864417483 1107 12-13 0.4760293304861136 1107 14-15 0.3134156218654667 1107 16-17 0.39454912915515905 1107 18-19 0.3885027670201211 1107 20-21 0.5964028088929823 1107 22-23 0.25669095775320017 1107 24-25 0.3871057628120198 1107 26-27 0.45203792211009386 1107 28-29 0.4643556051332993 1107 30-31 0.6968442103603607 1107 32-33 0.6049471477622888 1107 34-35 0.6774109157507766 1107 36-37 0.748612787488149 1107 38-39 1.0176322295949518 1107 40-41 0.9150242139845091 1107 42-43 0.8242209459435657 1107 44-45 0.7566628012793828 1107 46-47 0.8961239588183645 1107 48-49 0.7955581734607122 1107 50-51 0.5459341641783624 1107 52-53 0.9442727988397124 1107 54-55 0.9398952775699598 1107 56-57 1.0340185376391062 1107 58-59 0.7566704594077862 1107 60-61 0.7531393430881792 1107 62-63 0.6865647446296137 1107 64-65 0.44157549303151455 1107 66-67 0.5619741508692542 1107 68-69 0.8515641026678153 1107 70-71 1.0060765680256551 1107 72-73 0.8949760591244029 1107 74-75 0.9794880031143656 1107 76-77 0.8982773836127507 1107 78-79 0.874119286999413 1107 80-81 1.2368089978297903 1107 82-83 1.0286609920451433 1107 84-85 0.8361516033290606 1107 86-87 0.8924040908522102 1107 88-89 0.9326093697469666 1107 90-91 0.4724975501984474 1107 92-93 0.7734105547393391 1107 94-95 0.6905963937357171 1107 96-97 0.7987908467202445 1107 98-99 0.834276230120409 1107 100-101 1.2010434812725386 1108 1 0.02598070212174619 1108 2 -0.008272449620335465 1108 3 0.09344435188327438 1108 4 0.24994135952366037 1108 5 0.1291729133362267 1108 6 0.27367439526037884 1108 7 0.17761807491174864 1108 8 0.11704213139508823 1108 9 0.09781324084021747 1108 10-11 0.13178629488678695 1108 12-13 -0.016968294354576585 1108 14-15 0.2288078128050941 1108 16-17 0.3751061329785941 1108 18-19 0.38118177292905386 1108 20-21 0.23290205579369427 1108 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-0.7203567740701686 2316 82-83 -0.7399636133118186 2316 84-85 -0.5935128227835769 2316 86-87 -0.4070263137569299 2316 88-89 -0.7210977287355682 2316 90-91 -0.8590689826169395 2316 92-93 -1.186625017693114 2316 94-95 -1.3978045563173822 2316 96-97 -1.3378583974565004 2316 98-99 -1.2767248537839748 2316 100-101 -0.9974010431916938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1853.0 3 461.0 4 486.0 5 449.0 6 531.0 7 616.0 8 862.0 9 1059.0 10 1464.0 11 1969.0 12 2838.0 13 3907.0 14 4495.0 15 5968.0 16 8349.0 17 11653.0 18 9847.0 19 5466.0 20 4897.0 21 4799.0 22 5064.0 23 5499.0 24 5867.0 25 6552.0 26 7590.0 27 9049.0 28 10500.0 29 12739.0 30 15730.0 31 19554.0 32 24855.0 33 32780.0 34 45465.0 35 66731.0 36 97287.0 37 142545.0 38 138559.0 39 28885.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.89951168814471 20.007660844246463 15.247490805647596 19.84533666196123 2 14.861439018965209 22.327599375061972 41.07480229501255 21.736159310960264 3 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18.94651671063939 22-23 21.061772286193463 27.457013647396707 32.23762116284179 19.24359290356804 24-25 20.39438215716753 27.708279895604722 32.46674359467201 19.430594352555737 26-27 20.1946488312231 27.844934496529966 32.420400761774154 19.54001591047279 28-29 19.755356925191244 28.130537922757405 32.5050866846068 19.60901846744455 30-31 19.95745527078575 27.96656415489388 32.432019830102305 19.643960744218063 32-33 19.93547860067039 28.09640445194977 32.174590781930256 19.79352616544958 34-35 20.207413815267927 28.22195112806119 31.962872003085295 19.607763053585582 36-37 20.38724632867929 27.919312633414307 31.93158474101889 19.76185629688751 38-39 20.14420282673966 28.18189001052595 31.935944375848706 19.737962786885685 40-41 20.131414422903696 28.20266565939273 31.917791980518896 19.748127937184684 42-43 20.083552830288504 28.436090054360236 31.268153285556334 20.212203829794927 44-45 20.15281814961608 28.63127247793471 30.9930501289332 20.22285924351601 46-47 20.492405248877137 28.841777859303797 30.438787758689827 20.227029133129236 48-49 20.762156378843287 29.051780355629237 29.784785260629178 20.401278004898305 50-51 20.562991662159487 29.372011202801772 29.522823298828847 20.542173836209898 52-53 20.61725353244277 29.791695679653774 29.21208315032834 20.378967637575123 54-55 20.369890503251156 29.675607595470677 29.04372303676876 20.910778864509407 56-57 20.594212282378056 30.098301074073653 28.73691054341123 20.570576100137064 58-59 20.934171017140287 29.438889350201297 28.866006305396553 20.760933327261867 60-61 20.64892113212323 29.491465951863265 29.501912116948837 20.357700799064666 62-63 20.594347033253886 29.485578284957363 29.552612422811965 20.367462258976783 64-65 20.376753941703427 29.70970879831185 29.41420238373389 20.49933487625083 66-67 20.40568117052419 29.845500397483697 28.987377632283046 20.761440799709067 68-69 20.38085748513259 29.679040718055237 29.186931276772555 20.753170520039614 70-71 20.454928819476983 29.75237424595769 28.995520593755607 20.79717634080972 72-73 20.41783716090807 29.78413375394153 28.93026698982013 20.86776209533027 74-75 20.15685392476206 30.316345287487433 28.664264857678763 20.862535930071747 76-77 20.28131312488827 30.141734518772843 28.778488008907534 20.79846434743135 78-79 20.32448020015818 30.23019396351105 28.440838215961623 21.00448762036915 80-81 20.202426629050326 30.409385697175495 28.327007780129172 21.06117989364501 82-83 20.321225168999796 30.200656914510716 28.422434199732816 21.055683716756675 84-85 20.232065080547088 30.041425364698572 28.371017513031642 21.355492041722695 86-87 20.482030991533517 29.94497656682173 28.19596003702161 21.377032404623147 88-89 20.329781112743316 29.852518874730468 28.280177296199465 21.53752271632675 90-91 20.184458821898524 29.86935059699324 28.638665231748828 21.30752534935941 92-93 20.215901159947972 30.309417695134222 28.237948074947187 21.236733069970622 94-95 20.12424673574324 30.232188934350457 28.0222341904938 21.6213301394125 96-97 20.251257425981358 30.219155213393968 27.82616035564321 21.70342700498147 98-99 20.309537770778537 30.235989880882574 27.61071554222721 21.843756806111678 100-101 20.35331691972836 30.032809339939874 27.814962292043333 21.798911448288433 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1607.0 1 1045.0 2 535.0 3 627.5 4 673.0 5 758.5 6 1098.0 7 1822.5 8 2758.0 9 3141.0 10 2739.0 11 2395.0 12 2397.0 13 2495.0 14 2561.0 15 2616.0 16 2675.0 17 2807.5 18 2883.0 19 2913.5 20 3091.5 21 3415.0 22 3899.5 23 4500.0 24 5141.5 25 5978.5 26 7107.0 27 8184.5 28 9034.0 29 9991.5 30 11734.5 31 13681.5 32 15620.5 33 17278.0 34 19235.0 35 20800.0 36 22139.0 37 23941.0 38 24183.5 39 23463.0 40 23199.5 41 23687.0 42 23879.0 43 24098.5 44 24789.0 45 24937.0 46 24318.5 47 24474.0 48 26911.5 49 26601.5 50 23233.0 51 21077.5 52 19690.0 53 19165.5 54 18470.0 55 16554.5 56 14700.0 57 13173.5 58 11991.5 59 10828.0 60 9355.0 61 7850.5 62 6298.0 63 4704.0 64 3273.5 65 2432.0 66 1704.5 67 1242.0 68 851.0 69 465.0 70 297.0 71 221.5 72 167.0 73 119.5 74 89.5 75 75.5 76 52.5 77 21.5 78 11.5 79 12.5 80 10.5 81 7.5 82 4.5 83 4.0 84 4.5 85 4.0 86 2.0 87 1.5 88 1.5 89 2.0 90 2.0 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07735214765962914 2 0.12285341098882276 3 0.083374373688493 4 0.16139565757355145 5 0.12753736456682796 6 0.1299462549783735 7 0.14239218877135884 8 0.12258575649865101 9 0.14680848785919234 10-11 0.13282354074771957 12-13 0.11857093914607512 14-15 0.1231879791015374 16-17 0.14332897948695986 18-19 0.11957464348421908 20-21 0.09990203845659715 22-23 0.09582030748147831 24-25 0.0865193139480108 26-27 0.07414029377756841 28-29 0.0903333904329579 30-31 0.06537460922444435 32-33 0.06731510427818936 34-35 0.06209584171984069 36-37 0.06664596805276005 38-39 0.06771658601344696 40-41 0.0886605498693846 42-43 0.08544869598732388 44-45 0.07059387178279303 46-47 0.04918151256905486 48-49 0.05928546957303756 50-51 0.03693631964369834 52-53 0.05888398783777997 54-55 0.06657905443021712 56-57 0.0663113999400454 58-59 0.07628152969894224 60-61 0.07313658943942443 62-63 0.07996177893880348 64-65 0.050653612264999356 66-67 0.08839289537921288 68-69 0.07340424392959617 70-71 0.0345274292321528 72-73 0.02924125305126119 74-75 0.039479037300329756 76-77 0.060021519421009806 78-79 0.08330746006595006 80-81 0.05266102094128731 82-83 0.07487634362554066 84-85 0.07869042011048777 86-87 0.08578326410003854 88-89 0.10672722795597618 90-91 0.08237066935034902 92-93 0.098095370647938 94-95 0.10893537749989293 96-97 0.11589439424435784 98-99 0.14981960087362425 100-101 0.14761145132970752 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 747232.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.612861340034 #Duplication Level Percentage of deduplicated Percentage of total 1 79.1596807726406 45.606157120754986 2 12.021944857078173 13.852372841767593 3 3.7459122122581965 6.4743816263031455 4 1.6208764572373942 3.7353332232057417 5 0.839363194677907 2.4179057674453115 6 0.5001322685232981 1.7288430622865656 7 0.3510870315357233 1.4158989926306238 8 0.2813858717499461 1.2969156169739389 9 0.19009485728622813 0.9856717788856513 >10 1.1700617562206408 12.471481852119629 >50 0.0839960291098979 3.3228127627708046 >100 0.03289816794242611 3.235684574243183 >500 0.0011666016998131356 0.49131432500349365 >1k 9.332813598505085E-4 1.1283426659587756 >5k 4.666406799252543E-4 1.8368837896505446 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8091 1.0827962399897222 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5574 0.7459530641086035 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3740 0.5005138966211297 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2369 0.3170367436084108 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1210 0.1619309665538949 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1075 0.14386428846730334 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 990 0.13248897263500492 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 914 0.1223181020084793 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.691362254293178E-5 0.0 22-23 0.0 0.0 0.0 5.353089803434543E-4 0.0 24-25 0.0 0.0 0.0 0.0016059269410303627 0.0 26-27 0.0 0.0 0.0 0.0026765449017172715 0.0 28-29 0.0 0.0 0.0 0.005553830671063338 0.0 30-31 0.0 0.0 0.0 0.010371611494154425 0.0 32-33 0.0 0.0 0.0 0.015323219562331378 0.0 34-35 0.0 0.0 0.0 0.01840124619930624 0.0 36-37 0.0 0.0 0.0 0.02248297717442508 0.0 38-39 0.0 0.0 0.0 0.03118174810500621 0.0 40-41 0.0 0.0 0.0 0.04837854909853968 0.0 42-43 0.0 0.0 0.0 0.07815511113014433 0.0 44-45 0.0 0.0 0.0 0.10378302856408719 0.0 46-47 0.0 0.0 0.0 0.1669494882446148 0.0 48-49 0.0 0.0 0.0 0.22576656245985183 0.0 50-51 0.0 0.0 0.0 0.27026412145090145 0.0 52-53 0.0 0.0 0.0 0.32640465076442127 0.0 54-55 0.0 0.0 0.0 0.3858908612050876 0.0 56-57 0.0 0.0 0.0 0.5229299601730119 0.0 58-59 0.0 0.0 0.0 0.663113999400454 0.0 60-61 0.0 0.0 0.0 0.8114615005781337 0.0 62-63 0.0 0.0 0.0 0.9598090017558134 0.0 64-65 0.0 0.0 0.0 1.1223421909125948 0.0 66-67 0.0 0.0 0.0 1.3796250695901675 0.0 68-69 0.0 0.0 0.0 1.699806753458096 0.0 70-71 0.0 0.0 0.0 1.9050977474198107 0.0 72-73 0.0 0.0 0.0 2.10523639244572 0.0 74-75 0.0 6.691362254293178E-5 0.0 2.3564301314718854 0.0 76-77 0.0 1.3382724508586357E-4 0.0 2.6270957346580444 0.0 78-79 0.0 1.3382724508586357E-4 0.0 2.8897317031390517 0.0 80-81 0.0 1.3382724508586357E-4 0.0 3.311153697914436 0.0 82-83 0.0 1.3382724508586357E-4 0.0 3.8145448803049122 0.0 84-85 0.0 1.3382724508586357E-4 0.0 4.434231938675003 0.0 86-87 0.0 1.3382724508586357E-4 0.0 4.966596719626569 0.0 88-89 0.0 1.3382724508586357E-4 0.0 5.6000679842405034 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7745 0.0 36.09998 1 GGTATCA 4890 0.0 35.20066 1 TAGTCGA 75 3.0644872E-4 31.68941 5 TCGTTAC 30 0.003963394 31.646948 44-45 TATCAAC 9715 0.0 28.830536 2 ATCAACG 9725 0.0 28.74042 3 TCAACGC 9830 0.0 28.435335 4 CAACGCA 9905 0.0 28.12215 5 AACGCAG 10095 0.0 27.639942 6 GCGTCCT 70 0.008360386 27.16235 9 TATCGCG 45 8.94283E-4 26.391907 90-91 CGGGTCG 110 9.426133E-5 25.927696 6 GTACATG 6000 0.0 25.360033 1 GAGTACT 5015 0.0 25.301355 12-13 ACGCAGA 11045 0.0 25.090437 7 CGCAGAG 10950 0.0 24.960835 8 TACATGG 6045 0.0 24.858274 2 AGTACTT 5145 0.0 24.477324 12-13 GCAGAGT 11180 0.0 23.937124 9 ACATGGG 5990 0.0 23.648022 3 >>END_MODULE