FastQCFastQC Report
Thu 26 May 2016
SRR936835_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936835_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747232
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120831.6170346023724895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91811.2286679371333133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63190.8456543616975719No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23090.30900710890325894No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20640.2762194338572224No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15620.20903815682411891No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14710.1968598775213053No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11080.14828058755513682No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9200.12312106547899447No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8720.11669735771487302No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49400.043.1728361
GTATCAA85800.040.6903271
TATCAAC110750.031.3069822
ATCAACG110700.031.278223
TCAACGC112100.030.885524
CAACGCA113450.030.5180025
AACGCAG114900.030.0915376
CGCAGAG128200.026.932678
ACGCAGA128550.026.8223957
GCAGAGT133450.025.4816469
GAGTACT81950.023.99299412-13
AGTACTT82850.023.47439612-13
AGAGTAC117900.022.71947910-11
TTGCGTA753.0372688E-522.16357460-61
GTACTTT88750.022.15465514-15
GTACATG56200.021.8904461
TACCTGG4350.021.8374982
GTATAGA2005.381171E-721.374881
TACATGG57700.021.2376272
GTCTAGG1805.1650077E-621.1109921
Produced by FastQC (version 0.11.5)