##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936835_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 747232 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1085566463963 31.0 31.0 34.0 31.0 34.0 2 32.08047166074258 33.0 31.0 34.0 30.0 34.0 3 32.23024174553552 33.0 31.0 34.0 30.0 34.0 4 36.0105281893709 37.0 35.0 37.0 35.0 37.0 5 35.95409190184574 37.0 35.0 37.0 35.0 37.0 6 36.01968732602458 37.0 35.0 37.0 35.0 37.0 7 35.9555037792814 37.0 35.0 37.0 35.0 37.0 8 35.946818391075325 37.0 35.0 37.0 35.0 37.0 9 37.601384844332145 39.0 37.0 39.0 35.0 39.0 10-11 37.54479599374759 39.0 37.0 39.0 35.0 39.0 12-13 37.43740765920089 39.0 37.0 39.0 34.5 39.0 14-15 38.54139745085864 40.0 38.0 41.0 34.0 41.0 16-17 38.4883115284142 40.0 38.0 41.0 34.0 41.0 18-19 38.65007855659287 40.0 38.0 41.0 34.5 41.0 20-21 38.759765374073915 40.0 38.0 41.0 35.0 41.0 22-23 38.67436284848615 40.0 38.0 41.0 34.0 41.0 24-25 38.56342474626354 40.0 38.0 41.0 34.0 41.0 26-27 38.365013810971696 40.0 38.0 41.0 34.0 41.0 28-29 38.17108675752644 40.0 37.5 41.0 34.0 41.0 30-31 37.87099588882703 40.0 37.0 41.0 33.0 41.0 32-33 37.66919979337074 40.0 36.0 41.0 33.0 41.0 34-35 37.498891241274464 40.0 35.5 41.0 33.0 41.0 36-37 37.2593545244315 40.0 35.0 41.0 33.0 41.0 38-39 37.046461473812684 39.5 35.0 41.0 32.5 41.0 40-41 36.78436148344825 39.0 35.0 41.0 31.5 41.0 42-43 36.42601762237163 39.0 35.0 41.0 31.0 41.0 44-45 35.823409597019406 39.0 35.0 41.0 29.5 41.0 46-47 35.22441356901203 39.0 35.0 41.0 25.5 41.0 48-49 34.622221077255794 39.0 35.0 40.5 22.5 41.0 50-51 34.25566825296561 39.0 34.5 40.0 19.5 41.0 52-53 33.97619347137167 38.0 34.0 40.0 16.5 41.0 54-55 33.726708706265256 38.0 34.0 40.0 13.5 41.0 56-57 33.45026711918119 38.0 33.5 40.0 9.0 41.0 58-59 33.135003586570164 38.0 33.0 40.0 7.5 41.0 60-61 32.814787107618514 37.0 33.0 40.0 4.5 41.0 62-63 32.46215766990707 37.0 32.5 40.0 2.0 41.0 64-65 32.07185050961415 36.0 32.0 39.0 2.0 41.0 66-67 31.706756402295404 36.0 31.0 39.0 2.0 40.0 68-69 31.272349819065564 35.0 31.0 38.5 2.0 40.0 70-71 30.800554580103636 35.0 31.0 38.0 2.0 40.0 72-73 30.48316319429575 35.0 30.0 37.5 2.0 39.5 74-75 30.059030528671148 35.0 30.0 37.0 2.0 39.0 76-77 28.684563161106592 33.5 28.0 35.5 2.0 38.0 78-79 29.35518955290994 34.0 29.0 36.0 2.0 38.5 80-81 29.170512638645025 34.0 29.0 36.0 2.0 37.5 82-83 28.831558337972677 34.0 29.0 35.0 2.0 37.0 84-85 28.44667921074044 34.0 29.0 35.0 2.0 36.5 86-87 28.08912493040983 34.0 29.0 35.0 2.0 36.0 88-89 27.72703591387949 34.0 27.5 35.0 2.0 36.0 90-91 27.31074953963428 34.0 26.5 35.0 2.0 35.0 92-93 26.88446158622757 33.0 26.0 35.0 2.0 35.0 94-95 26.433031508286582 33.0 25.0 35.0 2.0 35.0 96-97 25.82169058605627 33.0 23.5 35.0 2.0 35.0 98-99 25.188909200890755 32.5 19.0 34.5 2.0 35.0 100-101 22.520418691918977 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.440051843258388 1101 2 0.2796040754898925 1101 3 0.33296421777036045 1101 4 0.24264741824352143 1101 5 0.2457863269422944 1101 6 0.2754848269045027 1101 7 0.3113540644079791 1101 8 0.2897409751351816 1101 9 0.32944997896898087 1101 10-11 0.3866881547543457 1101 12-13 0.3380671070050525 1101 14-15 0.4043688177105693 1101 16-17 0.5338089476625711 1101 18-19 0.49424093807804326 1101 20-21 0.4193626576809635 1101 22-23 0.374517250814705 1101 24-25 0.44271138158514134 1101 26-27 0.47397472009988206 1101 28-29 0.48975742422279467 1101 30-31 0.4268595827354744 1101 32-33 0.6383271790519842 1101 34-35 0.6500309203587236 1101 36-37 0.7329976085230498 1101 38-39 0.9989267414627605 1101 40-41 0.8525288842395966 1101 42-43 1.0114421810548535 1101 44-45 0.9330017343465684 1101 46-47 1.1717177985138605 1101 48-49 0.7836408354445155 1101 50-51 0.9112682267031502 1101 52-53 0.8349716795178637 1101 54-55 0.9661081119183308 1101 56-57 0.9708113512513776 1101 58-59 0.9401179609094683 1101 60-61 1.1669079540045502 1101 62-63 1.1836105292830013 1101 64-65 1.2563614271904804 1101 66-67 1.394560143698044 1101 68-69 1.1977126311976711 1101 70-71 1.184012276934208 1101 72-73 1.2526388394806247 1101 74-75 1.1731856140631685 1101 76-77 1.0441982384218171 1101 78-79 1.2509901153910796 1101 80-81 1.4467808071385875 1101 82-83 1.4596821970077407 1101 84-85 1.2848410277062108 1101 86-87 1.107022623449371 1101 88-89 1.0612909164777058 1101 90-91 1.2081720283422648 1101 92-93 1.2715344812671248 1101 94-95 1.3565882214194964 1101 96-97 1.5547699732703357 1101 98-99 1.6171265778742487 1101 100-101 1.5777279269083806 1102 1 0.23073243079998917 1102 2 0.19022210906193493 1102 3 0.23861991867559595 1102 4 0.17396330079517952 1102 5 0.18436136303959927 1102 6 0.15685102959100305 1102 7 0.2118066369091025 1102 8 0.24759201871567882 1102 9 0.38321181699018325 1102 10-11 0.29538423260634517 1102 12-13 0.33462981400779057 1102 14-15 0.5177161342493619 1102 16-17 0.5475947241318053 1102 18-19 0.5840348151628447 1102 20-21 0.5571101679147787 1102 22-23 0.3917204744786673 1102 24-25 0.4989490937257486 1102 26-27 0.4898967869564217 1102 28-29 0.3457889164242758 1102 30-31 0.5000328207148783 1102 32-33 0.5286407515522242 1102 34-35 0.3169535243749806 1102 36-37 0.5028304010215194 1102 38-39 0.6009857655255715 1102 40-41 0.7811929265561375 1102 42-43 0.8346681206654054 1102 44-45 1.1280793578955155 1102 46-47 1.393551092008579 1102 48-49 1.1317216581368257 1102 50-51 0.8103950995956382 1102 52-53 0.9359696146723664 1102 54-55 0.8368404925943409 1102 56-57 0.9974589718278608 1102 58-59 1.0183399710511196 1102 60-61 0.831561962283331 1102 62-63 1.020793801300158 1102 64-65 0.91165791907002 1102 66-67 1.0708931862536133 1102 68-69 0.7949614737463655 1102 70-71 0.8005450652871069 1102 72-73 0.8332273513188682 1102 74-75 0.7214885333366148 1102 76-77 0.6561876610500867 1102 78-79 0.753699728962431 1102 80-81 0.6231641257272251 1102 82-83 0.6870577616208244 1102 84-85 0.7308326607158655 1102 86-87 0.5880562104855613 1102 88-89 0.5872192369671261 1102 90-91 0.8261134276565691 1102 92-93 0.7272620239774952 1102 94-95 1.0095071861565934 1102 96-97 1.0926410567012041 1102 98-99 1.3574698308747095 1102 100-101 1.5169737511310224 1103 1 0.02048055211656674 1103 2 -0.2074338532214668 1103 3 0.014253527677837496 1103 4 0.1420661096133813 1103 5 0.1601416569728329 1103 6 0.10119734791399537 1103 7 0.2870416092674404 1103 8 0.21610587918972612 1103 9 0.20105855314614018 1103 10-11 0.24996250299695788 1103 12-13 0.26035674425524746 1103 14-15 0.34613284464677463 1103 16-17 0.3075155184421803 1103 18-19 0.4025909805725334 1103 20-21 0.16505139378767097 1103 22-23 0.29118600299824493 1103 24-25 0.21930147140918876 1103 26-27 0.2534763583150905 1103 28-29 0.40174827864738205 1103 30-31 0.39653283062601474 1103 32-33 0.3758458611121043 1103 34-35 0.41442023597216604 1103 36-37 0.6139744913193397 1103 38-39 0.569635773819634 1103 40-41 0.49488292892438324 1103 42-43 0.3636269184749352 1103 44-45 0.5290122573641654 1103 46-47 0.7904861489767967 1103 48-49 0.5327286303658028 1103 50-51 0.3797272032515977 1103 52-53 0.5527296721361381 1103 54-55 0.690245283537017 1103 56-57 0.7201263840907615 1103 58-59 0.7533629283904233 1103 60-61 0.8587007610735427 1103 62-63 0.8856730887828022 1103 64-65 0.6099491335190059 1103 66-67 0.5872048426979006 1103 68-69 0.9763833994920752 1103 70-71 0.7105070169269823 1103 72-73 0.8863573427535485 1103 74-75 0.9538224408207832 1103 76-77 0.8782875299100041 1103 78-79 0.9572120297353983 1103 80-81 0.930522784989396 1103 82-83 1.0091840254220905 1103 84-85 0.9969400954301193 1103 86-87 0.863047681685341 1103 88-89 1.0127515958089646 1103 90-91 1.1132606561355622 1103 92-93 1.1303336439904967 1103 94-95 1.335940102839345 1103 96-97 1.4465476645130657 1103 98-99 1.1612928199296775 1103 100-101 1.0095315252758716 1104 1 0.2288695681159325 1104 2 0.11872244991841541 1104 3 0.08052934685087365 1104 4 0.1478212493130897 1104 5 0.16940256353490923 1104 6 0.14882723161135658 1104 7 0.2830462486851175 1104 8 0.31507552575822473 1104 9 0.34264078213533367 1104 10-11 0.269262839092697 1104 12-13 0.33972747151569394 1104 14-15 0.39903792828454954 1104 16-17 0.2792811313100856 1104 18-19 0.42456684291257574 1104 20-21 0.26657589209202115 1104 22-23 0.32845787645964464 1104 24-25 0.3485031854009506 1104 26-27 0.32066792461504434 1104 28-29 0.34759162796490983 1104 30-31 0.5004356510995862 1104 32-33 0.33673490814205564 1104 34-35 0.2933044036145702 1104 36-37 0.18422498401611875 1104 38-39 0.2566860888835336 1104 40-41 0.24539631276172003 1104 42-43 -0.11996272751432713 1104 44-45 -0.06734806154752704 1104 46-47 -0.21462010665180742 1104 48-49 -0.18849325764549718 1104 50-51 -0.27016915458486324 1104 52-53 -0.33254265919882187 1104 54-55 -0.09415763968227964 1104 56-57 -0.28775952264370375 1104 58-59 -0.31557117781141386 1104 60-61 -0.36124128757965224 1104 62-63 -0.30848874745569077 1104 64-65 -0.4926786745768048 1104 66-67 -0.2652860860269115 1104 68-69 -0.33747858472783676 1104 70-71 -0.22375295871745493 1104 72-73 -0.25770112714884874 1104 74-75 -0.0861408189631625 1104 76-77 -0.2526712049246562 1104 78-79 -0.25021434847092294 1104 80-81 -0.03482983971563769 1104 82-83 0.0017235825259582782 1104 84-85 0.08742322099642763 1104 86-87 -0.007939546124685393 1104 88-89 0.24469006973844287 1104 90-91 0.15176685742616058 1104 92-93 0.4783699594322819 1104 94-95 0.23383085034551954 1104 96-97 0.5027150972904657 1104 98-99 0.6337197291038379 1104 100-101 0.8872034312849131 1105 1 0.12394233591454196 1105 2 -0.09480318270253818 1105 3 -0.07785708730846608 1105 4 -0.013552816505708165 1105 5 0.08892181531585663 1105 6 0.028460114789538693 1105 7 0.0753752552844773 1105 8 0.12503837703221166 1105 9 0.0821346853738234 1105 10-11 0.20282430930816275 1105 12-13 0.2869133462589275 1105 14-15 0.21543756116773238 1105 16-17 0.22622400968078438 1105 18-19 0.20361758370187744 1105 20-21 0.16778825169168243 1105 22-23 0.11380729300612558 1105 24-25 0.09811727569420015 1105 26-27 -0.019735903888957296 1105 28-29 0.23221467765679904 1105 30-31 0.3885751554918784 1105 32-33 0.17958530803716855 1105 34-35 0.13963279835256515 1105 36-37 -0.09318771647774327 1105 38-39 0.08767337090792182 1105 40-41 -0.15922761101783323 1105 42-43 -0.03814637333400839 1105 44-45 0.05819540246191224 1105 46-47 0.1453763969975057 1105 48-49 0.09293500160890744 1105 50-51 0.06781170289513483 1105 52-53 -0.11153658428951019 1105 54-55 -0.25850185540711124 1105 56-57 -0.19827217506274764 1105 58-59 -0.21502399892937518 1105 60-61 -0.3755448582277907 1105 62-63 -0.39087413270982907 1105 64-65 -0.2745264959132818 1105 66-67 -0.4235789928054601 1105 68-69 -0.40473700874776597 1105 70-71 -0.45477083381032557 1105 72-73 -0.4839604689608308 1105 74-75 -0.4712175201562907 1105 76-77 -0.5540024404475794 1105 78-79 -0.6243999172834762 1105 80-81 -0.3070015105342243 1105 82-83 -0.21877289927116905 1105 84-85 -0.1747427682522975 1105 86-87 -0.3054247958952381 1105 88-89 0.07638973321468967 1105 90-91 0.16223667954931997 1105 92-93 -0.037204534689479374 1105 94-95 0.103171351197755 1105 96-97 0.3383070731656481 1105 98-99 -0.21173108161248066 1105 100-101 -0.17285862893972848 1106 1 0.27715426927370856 1106 2 0.17303099009870238 1106 3 0.17864814322233258 1106 4 0.2251143667565927 1106 5 0.16210275293693854 1106 6 0.17201687573490432 1106 7 0.1473318612511605 1106 8 0.2615901011701425 1106 9 0.3085740499611944 1106 10-11 0.29181307335310436 1106 12-13 0.24605321452703777 1106 14-15 0.20360493815339709 1106 16-17 0.36915310692990744 1106 18-19 0.33338899013349277 1106 20-21 0.369748732125629 1106 22-23 0.48990957894181264 1106 24-25 0.4572726418313593 1106 26-27 0.4061765331509548 1106 28-29 0.5477667892414573 1106 30-31 0.5245224627371314 1106 32-33 0.6466678341898202 1106 34-35 0.7082728603440458 1106 36-37 0.829353934045308 1106 38-39 0.7554300543639485 1106 40-41 0.6484438341584564 1106 42-43 0.7979094189945499 1106 44-45 0.5977692110632304 1106 46-47 0.584787477973876 1106 48-49 0.6355308946736073 1106 50-51 0.5427671465216406 1106 52-53 0.49185744903090267 1106 54-55 0.6753842352554216 1106 56-57 0.6781865618609331 1106 58-59 0.38725418781994847 1106 60-61 0.32979415765752407 1106 62-63 0.24913361622468955 1106 64-65 0.4953533956016365 1106 66-67 0.5520506081244108 1106 68-69 0.8573861993111258 1106 70-71 0.6037801154341516 1106 72-73 1.0850980354948057 1106 74-75 0.9076046417964392 1106 76-77 0.806036304225028 1106 78-79 0.716994890930394 1106 80-81 0.9792286328210658 1106 82-83 0.9973758802716439 1106 84-85 0.8237926831231412 1106 86-87 1.0563574590447082 1106 88-89 1.1405431621182167 1106 90-91 1.1223839093052277 1106 92-93 0.9192309554306277 1106 94-95 0.940064028454632 1106 96-97 1.124366739961463 1106 98-99 0.9910430369462198 1106 100-101 1.30590154153683 1107 1 0.05465114817508265 1107 2 0.03508734822247561 1107 3 0.10614948083603082 1107 4 -0.03452982799996107 1107 5 0.08134655532681023 1107 6 -0.14612116599864322 1107 7 0.023714062071562125 1107 8 0.08738375196379167 1107 9 0.30559117141651626 1107 10-11 0.10187192835578429 1107 12-13 0.0558200238725064 1107 14-15 0.014600122744091948 1107 16-17 0.10105296425111732 1107 18-19 0.1096753286389287 1107 20-21 0.022807408779812022 1107 22-23 -0.03273622095118611 1107 24-25 -0.05145305818647472 1107 26-27 -0.014291185771824644 1107 28-29 -0.14686715047675847 1107 30-31 0.05406366123900597 1107 32-33 0.08408311865566986 1107 34-35 -0.22183682397912463 1107 36-37 -0.04548109359870267 1107 38-39 0.13693308628833023 1107 40-41 0.13334217551747685 1107 42-43 0.13351892496923057 1107 44-45 0.278833059823711 1107 46-47 0.12420918298546724 1107 48-49 0.2057935128294801 1107 50-51 0.4488844998300081 1107 52-53 0.49795027942148806 1107 54-55 0.3203815600280251 1107 56-57 0.41156225296133897 1107 58-59 0.38036324792339116 1107 60-61 0.5622000788274946 1107 62-63 0.5643913297970116 1107 64-65 0.39358105745288796 1107 66-67 -0.0411966720610728 1107 68-69 0.4143886070157805 1107 70-71 0.45773053455311086 1107 72-73 0.28406361423566295 1107 74-75 0.40291630578787974 1107 76-77 0.28113439589615297 1107 78-79 0.1397722228369389 1107 80-81 0.19841447195072703 1107 82-83 0.4071493776855952 1107 84-85 0.3349260877958713 1107 86-87 0.2174172949640969 1107 88-89 0.3089554146596818 1107 90-91 0.407915387814235 1107 92-93 0.5836192200997914 1107 94-95 0.7413637430970432 1107 96-97 0.769787642404836 1107 98-99 0.7892171942495914 1107 100-101 0.6184372112354204 1108 1 0.04238431930241404 1108 2 -0.13464964633203635 1108 3 0.008304503429009458 1108 4 0.17319523282728966 1108 5 0.17678740806692872 1108 6 0.08067758269791625 1108 7 0.08116684853894895 1108 8 0.07618864131612213 1108 9 0.06832144600810608 1108 10-11 -0.039073500910809855 1108 12-13 -0.049099742087413745 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-1.009900886912483 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 8.0 5 21.0 6 61.0 7 120.0 8 303.0 9 562.0 10 1143.0 11 1849.0 12 3039.0 13 4156.0 14 5285.0 15 7844.0 16 10815.0 17 16302.0 18 14198.0 19 7029.0 20 5198.0 21 4884.0 22 4995.0 23 5177.0 24 5685.0 25 6652.0 26 7851.0 27 9303.0 28 11437.0 29 14040.0 30 17213.0 31 21455.0 32 26770.0 33 34466.0 34 47670.0 35 68602.0 36 98235.0 37 149295.0 38 123620.0 39 11946.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.46682746749405 19.81116164766415 16.16182167170396 19.56018921313784 2 15.25285361163045 22.25743464697388 40.823274817956374 21.666436923439296 3 18.28933228232227 28.116330329743565 32.427661576222164 21.166675811711993 4 11.903398141407221 18.63999400453942 38.77149265556079 30.685115198492568 5 11.757061852013935 38.978666227705666 33.93596790231512 15.328304017965284 6 27.71055153179485 38.73275626716344 17.97818068916047 15.578511511881246 7 24.817091367997314 36.92780411947577 21.36327320467165 16.891831307855266 8 26.471965868699414 33.348143548456164 20.199616718770073 19.980273864074345 9 27.586479165774485 16.172621086891354 21.177626225857566 35.063273521476596 10-11 23.755572239374438 28.111320327984252 27.015206810209964 21.11790062243135 12-13 26.461761440854044 26.485582961456643 28.227297455179073 18.82535814251024 14-15 23.01641257333733 26.094305383067105 29.482409746905912 21.40687229668965 16-17 19.274870455226754 29.87947518307567 29.458722324525716 21.386932037171853 18-19 19.129667894308593 26.278719326795425 35.5531481521134 19.038464626782577 20-21 20.76994213155035 24.920036080790993 35.81333008570526 18.496691701953395 22-23 20.05528403494497 25.424834589525076 35.74084086334632 18.77904051218363 24-25 19.82632401672386 25.311364086531512 36.13402641341763 18.728285483327 26-27 19.568571872122654 25.5707854221537 36.05369799361898 18.806944712104663 28-29 19.095744040170715 25.896634284024188 36.03808027451416 18.969541401290936 30-31 19.326223809453694 25.70772869585703 35.929822119123564 19.036225375565706 32-33 19.41268064325963 25.72463065031908 35.70896603501888 19.153722671402406 34-35 19.540532926666877 25.69051549523664 35.620609103020755 19.14834247507573 36-37 19.778276979122545 25.489180513481447 35.551567298163064 19.18097520923295 38-39 19.54882740225787 25.928974309044932 35.45395963777179 19.068238650925412 40-41 19.406539667074416 26.04691686496412 35.248743967430144 19.29779950053132 42-43 19.564245660523817 26.02688668513537 34.73869461061817 19.67017304372265 44-45 19.801533664333608 26.252425625309478 34.12229166387859 19.823749046478326 46-47 20.113820376590876 26.75314160298703 33.35927358376939 19.773764436652705 48-49 20.522421766213014 27.049916926682027 32.30190376074884 20.125757546356116 50-51 20.19830547715499 27.379485058837226 32.014865550278294 20.407343913729488 52-53 20.347554936966354 27.824937769331655 31.387543159979657 20.43996413372233 54-55 20.163101375260304 27.63636168971258 31.389955085893543 20.81058184913357 56-57 20.327878725020156 28.092916815829184 30.93656215811115 20.64264230103951 58-59 20.68094935705142 27.417171481280985 31.26241683356274 20.63946232810485 60-61 20.497402711244256 27.30963977883399 31.828045633055257 20.364911876866497 62-63 20.292961222217464 27.473127489186762 31.974540704894867 20.25937058370091 64-65 20.242522707228975 27.561764867262916 31.775198767713782 20.42051365779433 66-67 20.348995156785517 27.71425436043205 31.265498888563243 20.67125159421919 68-69 20.481590724165986 27.50946158622757 31.392325275148814 20.616622414457623 70-71 20.576783201435166 27.743313475560754 31.098636899858878 20.581266423145202 72-73 20.51738951222646 27.62495449873667 31.07676063123635 20.78089535780052 74-75 20.23973941019341 27.991523336919922 30.860058777240685 20.908678475645985 76-77 20.144480860568475 28.034720372791668 30.913096020352583 20.907702746287274 78-79 20.053517622741577 27.997786492922867 30.808632677600823 21.140063206734734 80-81 19.986669798461005 28.25259928892208 30.47015058711106 21.290580325505857 82-83 20.081395691194224 27.9664192587398 30.50678921968807 21.44539583037791 84-85 19.741740258714778 27.778814992368105 30.592276297906768 21.887168451010346 86-87 19.87421324728961 27.701792116614048 30.385684479876662 22.03831015621968 88-89 19.727938042302164 27.635922242393303 30.246333239429646 22.38980647587489 90-91 19.497384342994334 27.534457216041623 30.626004542102773 22.342153898861262 92-93 19.287717870755 27.615185109845402 30.64730900175581 22.449788017643783 94-95 19.202909945458597 27.655656454066047 30.45151395720598 22.68991964326938 96-97 19.462269895545457 27.53116572873939 30.299110697724217 22.70745367799094 98-99 19.32246216067735 27.440041594982905 30.102227566579742 23.135268677760003 100-101 18.91791701434172 27.1413381904124 30.175629712851077 23.765115082394807 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1763.0 1 1224.0 2 792.0 3 874.5 4 697.5 5 636.0 6 1096.0 7 2393.0 8 4109.5 9 4671.5 10 3665.5 11 2680.0 12 2571.0 13 2649.5 14 2749.0 15 2948.0 16 3032.5 17 3090.0 18 3240.5 19 3317.0 20 3451.5 21 3777.5 22 4132.0 23 4764.5 24 5455.5 25 6212.5 26 7211.5 27 8145.5 28 8973.5 29 9918.0 30 11468.0 31 13252.0 32 15005.5 33 16573.0 34 18395.5 35 19940.0 36 21466.0 37 23332.0 38 23607.5 39 23067.5 40 23168.5 41 23401.0 42 23288.5 43 23902.5 44 24682.5 45 24831.5 46 24319.0 47 24461.0 48 27001.0 49 26231.0 50 22412.0 51 20704.0 52 19714.0 53 19353.5 54 18461.5 55 16381.0 56 14440.0 57 12894.0 58 11965.0 59 10814.0 60 9410.5 61 7786.0 62 6158.5 63 4681.5 64 3254.0 65 2367.0 66 1720.5 67 1319.0 68 905.0 69 506.5 70 332.0 71 225.5 72 176.5 73 147.0 74 106.5 75 90.0 76 71.5 77 40.5 78 18.0 79 6.5 80 4.0 81 3.5 82 3.0 83 2.5 84 1.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01846815982184917 2 0.002810372146803135 3 0.002007408676287953 4 0.0 5 0.0017397541861162262 6 5.353089803434543E-4 7 1.3382724508586357E-4 8 0.0 9 0.0 10-11 1.3382724508586357E-4 12-13 0.0011375315832298403 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002676544901717271 22-23 0.0 24-25 0.004817780823091088 26-27 0.002141235921373817 28-29 0.002810372146803135 30-31 0.021278531968652304 32-33 8.698770930581131E-4 34-35 0.002475804034088476 36-37 0.005152348935805746 38-39 0.0031449402595177937 40-41 0.005085435313262815 42-43 0.002944199391888998 44-45 2.6765449017172714E-4 46-47 2.6765449017172714E-4 48-49 6.691362254293178E-5 50-51 1.3382724508586357E-4 52-53 0.0016059269410303627 54-55 0.00374716286240418 56-57 6.02222602886386E-4 58-59 0.0014051860734015675 60-61 0.0014051860734015675 62-63 0.0 64-65 4.014817352575907E-4 66-67 1.3382724508586357E-4 68-69 0.0 70-71 2.0074086762879534E-4 72-73 0.0 74-75 5.353089803434543E-4 76-77 6.691362254293178E-4 78-79 2.0074086762879534E-4 80-81 0.007561239347351291 82-83 0.002408890411545544 84-85 0.005152348935805746 86-87 0.002007408676287953 88-89 0.0016728405635732947 90-91 1.3382724508586357E-4 92-93 0.0 94-95 2.0074086762879534E-4 96-97 0.0022750631664596806 98-99 0.003546421994775384 100-101 8.698770930581131E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 747232.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.892616139013015 #Duplication Level Percentage of deduplicated Percentage of total 1 77.83700615389492 41.94839894061853 2 12.479847539651507 13.451432658556895 3 3.970116362578279 6.418798714669374 4 1.8297017818319539 3.944296631085505 5 0.9433225267871749 2.5419059415712524 6 0.6081330934649203 1.9664330020521283 7 0.40832954631446344 1.5404163248419902 8 0.2861739119807655 1.2338128629903276 9 0.21206757568895626 1.0285988806922406 >10 1.2844858179213097 12.717571538584775 >50 0.09212957247344593 3.4151534763183418 >100 0.04444168666371752 4.091548029812355 >500 0.0022470515727619016 0.8446016502983201 >1k 0.001248361984867723 1.1456011551122982 >5k 4.993447939470893E-4 2.0856022908433904 >10k+ 2.4967239697354463E-4 1.6258279019523023 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12083 1.6170346023724895 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9181 1.2286679371333133 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6319 0.8456543616975719 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2309 0.30900710890325894 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2064 0.2762194338572224 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1562 0.20903815682411891 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1471 0.1968598775213053 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1108 0.14828058755513682 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 920 0.12312106547899447 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 872 0.11669735771487302 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.691362254293178E-5 0.0 22-23 0.0 0.0 0.0 7.360498479722496E-4 0.0 24-25 0.0 0.0 0.0 0.0019404950537450219 0.0 26-27 0.0 0.0 0.0 0.00301111301443193 0.0 28-29 0.0 0.0 0.0 0.006089139651406792 0.0 30-31 0.0 0.0 0.0 0.011174574964669608 0.0 32-33 0.0 0.0 0.0 0.016326923900475354 0.0 34-35 0.0 0.0 0.0 0.019404950537450217 0.0 36-37 0.0 0.0 0.0 0.02335285426748319 0.0 38-39 0.0 0.0 0.0 0.032453106933321915 0.0 40-41 0.0 0.0 0.0 0.04978373517194124 0.0 42-43 0.0 0.0 0.0 0.07976103807117468 0.0 44-45 0.0 0.0 0.0 0.10579043724037515 0.0 46-47 0.0 0.0 0.0 0.1688230696758169 0.0 48-49 0.0 0.0 0.0 0.2284431073615691 0.0 50-51 0.0 0.0 0.0 0.27340906171041923 0.0 52-53 0.0 0.0 0.0 0.32981724551411074 0.0 54-55 0.0 0.0 0.0 0.39030716029292106 0.0 56-57 0.0 0.0 0.0 0.5293536679371333 0.0 58-59 0.0 0.0 0.0 0.6710098068605199 0.0 60-61 0.0 0.0 0.0 0.8214316303370306 0.0 62-63 0.0 0.0 0.0 0.9705151813626826 0.0 64-65 0.0 0.0 0.0 1.1332491113870926 0.0 66-67 0.0 0.0 0.0 1.3902643355744937 0.0 68-69 0.0 0.0 0.0 1.7129218234765107 0.0 70-71 0.0 0.0 0.0 1.9199525716243415 0.0 72-73 0.0 0.0 0.0 2.1217640572138237 0.0 74-75 0.0 0.0 0.0 2.374630636803563 0.0 76-77 0.0 0.0 0.0 2.6465006851954946 0.0 78-79 0.0 0.0 0.0 2.9099396171470175 0.0 80-81 0.0 0.0 0.0 3.3324991435056313 0.0 82-83 0.0 0.0 0.0 3.8402397113613977 0.0 84-85 0.0 0.0 0.0 4.4622687465204915 0.0 86-87 0.0 0.0 0.0 4.995637231810202 0.0 88-89 0.0 0.0 0.0 5.630179114384823 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4940 0.0 43.172836 1 GTATCAA 8580 0.0 40.690327 1 TATCAAC 11075 0.0 31.306982 2 ATCAACG 11070 0.0 31.27822 3 TCAACGC 11210 0.0 30.88552 4 CAACGCA 11345 0.0 30.518002 5 AACGCAG 11490 0.0 30.091537 6 CGCAGAG 12820 0.0 26.93267 8 ACGCAGA 12855 0.0 26.822395 7 GCAGAGT 13345 0.0 25.481646 9 GAGTACT 8195 0.0 23.992994 12-13 AGTACTT 8285 0.0 23.474396 12-13 AGAGTAC 11790 0.0 22.719479 10-11 TTGCGTA 75 3.0372688E-5 22.163574 60-61 GTACTTT 8875 0.0 22.154655 14-15 GTACATG 5620 0.0 21.890446 1 TACCTGG 435 0.0 21.837498 2 GTATAGA 200 5.381171E-7 21.37488 1 TACATGG 5770 0.0 21.237627 2 GTCTAGG 180 5.1650077E-6 21.110992 1 >>END_MODULE