FastQCFastQC Report
Thu 26 May 2016
SRR936834_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936834_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552322
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62471.1310431233954108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43660.7904809151183548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29330.5310308117366319No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22170.4013962869485554No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.17363059954157176No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9170.1660263397076343No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9100.16475896306864474No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.12637555628781763No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61200.036.2610931
GGTATCA37650.032.9420781
GTAAGGT1401.695662E-830.5485044
TATCAAC76200.029.060722
ATCAACG76200.028.9983583
TCAACGC76850.028.6294214
CAACGCA77050.028.4960165
AACGCAG77800.028.1602256
GTACATG46900.025.2291131
CGCAGAG86300.024.8910058
ACGCAGA87100.024.8238247
AGTACTT40700.024.5772412-13
TAAGGTG1951.5576006E-824.3713845
TACATGG47450.024.3357932
GCAGAGT86700.024.0087649
GAGTACT39800.023.87934312-13
GGTATAG1000.001648537323.7599451
ACATGGG47100.023.4068263
CATGGGA33200.023.1874164
GTAATAC1050.002189450722.6285213
Produced by FastQC (version 0.11.5)