FastQCFastQC Report
Thu 26 May 2016
SRR936834_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936834_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552322
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97231.7603861515565196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74141.3423329144955298No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50780.9193912246841516No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19530.3535980822780914No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16290.2949366492734311No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13590.24605212176954747No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13470.2438794761027082No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7590.1374198384275839No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7170.12981557859364645No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6760.12239237256527893No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6690.12112499592628936No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6380.11551232795362126No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5580.10102802350802612No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT352.7897168E-454.2737166
GGTATCA38400.046.4184841
GTATCAA64850.038.5537571
TATCAAC83100.030.0132582
ATCAACG83800.029.703173
TCAACGC84300.029.577994
CAACGCA85700.029.0393815
AACGCAG87000.028.5482856
CCGTTGC700.00839691327.1368589
CTGGTCG1301.0025964E-525.5712689
ACGCAGA96650.025.353947
CGCAGAG96350.025.137158
TATAGAC1351.3405601E-524.6308753
GAGTACT61750.024.49569312-13
GCAGAGT98650.024.1178289
GTACATG39950.023.796011
CGTCACT1000.001645988223.7662641
AGTACTT62250.023.00197812-13
GTACTTT65850.022.86337714-15
TACATGG41750.022.7577022
Produced by FastQC (version 0.11.5)