FastQCFastQC Report
Thu 26 May 2016
SRR936833_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936833_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583143
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64681.1091619036840021No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46360.7950022550214956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29850.5118813052716058No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21470.3681772738419221No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10660.18280250298811784No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.15399310289242946No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8810.1510778659779848No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7300.12518370279674113No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGG500.001611739738.016411
GTATCAA63300.036.710161
GGTATCA38950.034.4050941
ATCAACG77700.029.8455733
TATCAAC78150.029.7345242
TCAACGC78750.029.3872854
CAACGCA79500.029.0502745
AACGCAG80950.028.5323686
GTCGGGA1205.357684E-627.7202992
GCGTCAG700.00837058727.154581
GTGTAGG1754.9276423E-927.1545751
CGGCAGC908.915251E-426.402556
GAGTACT41000.026.24880412-13
GTACATG47800.026.0468081
ACGCAGA87800.026.033457
CGCAGAG87450.025.865948
TACATGG48450.025.1088772
ACATGGG48250.024.8190043
GCAGAGT88750.024.6838939
GTACTTT44750.024.15018314-15
Produced by FastQC (version 0.11.5)