##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936833_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 583143 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.939026962511768 33.0 31.0 34.0 30.0 34.0 2 32.01916511044461 33.0 31.0 34.0 30.0 34.0 3 32.008606808278586 33.0 31.0 34.0 30.0 34.0 4 35.583783051498514 37.0 35.0 37.0 33.0 37.0 5 35.59256820368246 37.0 35.0 37.0 33.0 37.0 6 35.739828824147764 37.0 35.0 37.0 35.0 37.0 7 35.69502506246324 37.0 35.0 37.0 33.0 37.0 8 35.66325755432201 37.0 35.0 37.0 33.0 37.0 9 37.27259351479826 39.0 37.0 39.0 34.0 39.0 10-11 37.173757551749745 39.0 37.0 39.0 33.5 39.0 12-13 37.08156918628879 39.0 37.0 39.0 33.0 39.0 14-15 38.210268321835294 40.0 38.0 41.0 33.0 41.0 16-17 38.180498608403084 40.0 38.0 41.0 33.0 41.0 18-19 38.20823022826305 40.0 38.0 41.0 33.5 41.0 20-21 38.15546015299849 40.0 38.0 41.0 33.5 41.0 22-23 38.029681398902156 40.0 38.0 41.0 33.0 41.0 24-25 38.09432849232521 40.0 38.0 41.0 33.0 41.0 26-27 38.017127874294985 40.0 38.0 41.0 33.0 41.0 28-29 37.91698948628381 40.0 38.0 41.0 33.0 41.0 30-31 37.66588984177123 40.0 37.0 41.0 33.0 41.0 32-33 37.3723589239689 40.0 36.0 41.0 33.0 41.0 34-35 37.149222403424204 39.5 36.0 41.0 32.0 41.0 36-37 36.93242823801366 39.0 35.0 41.0 31.0 41.0 38-39 36.744466623109595 39.0 35.0 41.0 31.0 41.0 40-41 36.51280732170325 39.0 35.0 41.0 30.5 41.0 42-43 36.20170095499732 39.0 35.0 41.0 30.0 41.0 44-45 35.792370824994904 39.0 35.0 40.5 29.5 41.0 46-47 35.242578578496186 39.0 35.0 40.0 26.5 41.0 48-49 34.80024024981866 38.5 35.0 40.0 24.0 41.0 50-51 33.98953944401288 38.0 34.0 39.5 22.5 40.5 52-53 34.142231836787886 38.0 34.5 40.0 23.0 40.5 54-55 34.51584602747525 39.0 35.0 40.5 22.5 41.0 56-57 34.41066599444733 39.0 35.0 41.0 21.5 41.0 58-59 34.164415932284186 38.0 34.0 40.5 20.0 41.0 60-61 33.901314085910315 38.0 34.0 40.0 18.5 41.0 62-63 33.58657224728755 38.0 34.0 40.0 16.0 41.0 64-65 33.249700330793644 37.0 33.5 40.0 12.0 41.0 66-67 32.87373165758656 36.5 33.0 40.0 8.0 41.0 68-69 32.46825821453743 36.0 33.0 39.0 6.5 41.0 70-71 32.08289561908486 35.5 32.5 39.0 2.0 41.0 72-73 31.681065364756158 35.0 32.0 38.5 2.0 40.0 74-75 31.2497689246034 35.0 31.5 37.5 2.0 40.0 76-77 30.83316013396371 35.0 31.0 37.0 2.0 39.0 78-79 30.419682650739183 35.0 31.0 36.5 2.0 39.0 80-81 29.98023812341055 35.0 30.5 36.0 2.0 38.5 82-83 29.575426610625527 34.5 30.0 35.5 2.0 37.0 84-85 29.184402453600576 34.0 30.0 35.0 2.0 37.0 86-87 28.82353385018769 34.0 29.0 35.0 2.0 36.5 88-89 28.456195993092603 34.0 29.0 35.0 2.0 36.0 90-91 28.06446446240459 34.0 29.0 35.0 2.0 36.0 92-93 27.717221161876246 34.0 28.0 35.0 2.0 35.0 94-95 27.342489749512552 34.0 27.0 35.0 2.0 35.0 96-97 26.873324381841158 34.0 25.5 35.0 2.0 35.0 98-99 26.278243929876545 34.0 25.0 35.0 2.0 35.0 100-101 24.055555841363095 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3804250803582008 1101 2 0.2977180939777462 1101 3 0.22976649889720946 1101 4 0.30140707379801057 1101 5 0.12834361602278221 1101 6 0.12310867995613961 1101 7 0.06440465060271094 1101 8 0.11067314494727043 1101 9 0.24576333189558142 1101 10-11 0.2734032818413965 1101 12-13 0.2906064036264908 1101 14-15 0.35928260290928193 1101 16-17 0.3893675326251369 1101 18-19 0.40126194498740375 1101 20-21 0.5406746118729657 1101 22-23 0.4871172189233164 1101 24-25 0.48577696250364255 1101 26-27 0.5462358890955343 1101 28-29 0.564017085108631 1101 30-31 0.6880853394140232 1101 32-33 0.5684108912299024 1101 34-35 0.6626909144423578 1101 36-37 0.7414146310110041 1101 38-39 0.7142743643119687 1101 40-41 0.6551784530140736 1101 42-43 0.5447398443208868 1101 44-45 0.5828177878632985 1101 46-47 0.9162317291136617 1101 48-49 0.8354579075713247 1101 50-51 1.0054563112827637 1101 52-53 1.0055051104713328 1101 54-55 1.0046312386866063 1101 56-57 0.7941079433639899 1101 58-59 0.798025745789765 1101 60-61 1.1570968069151206 1101 62-63 1.2135224226269798 1101 64-65 1.4241938419345033 1101 66-67 1.1983466540112815 1101 68-69 1.1603153726096593 1101 70-71 0.9845067865943449 1101 72-73 0.8803828355954195 1101 74-75 1.057296057373975 1101 76-77 1.0066601699993036 1101 78-79 1.036216740010886 1101 80-81 1.0282144621201184 1101 82-83 0.9986576765509092 1101 84-85 0.8067788827749673 1101 86-87 0.879696514627966 1101 88-89 0.9062836718938279 1101 90-91 1.100275818774488 1101 92-93 0.8991268142637239 1101 94-95 0.9253985170688885 1101 96-97 0.6265663675690227 1101 98-99 0.6123347878727152 1101 100-101 0.8312099429020918 1102 1 0.3432951436684277 1102 2 0.3572337205479421 1102 3 0.11832175297552894 1102 4 0.2239100048267204 1102 5 0.2978729765560644 1102 6 0.1736979004699961 1102 7 0.2968208231816831 1102 8 0.3554271060232068 1102 9 0.5356464257219926 1102 10-11 0.38326292743815316 1102 12-13 0.4027994830489874 1102 14-15 0.31282094076477307 1102 16-17 0.5774283640323645 1102 18-19 0.5547567361306704 1102 20-21 0.5189789698939364 1102 22-23 0.5242974018195099 1102 24-25 0.2251679784819416 1102 26-27 0.4302354871258842 1102 28-29 0.4531645744731847 1102 30-31 0.39490341464934176 1102 32-33 0.5954299512908676 1102 34-35 0.30602592264924766 1102 36-37 0.1528660764268679 1102 38-39 0.3965997587446921 1102 40-41 0.5671102521632392 1102 42-43 0.7699245828731236 1102 44-45 0.6938096479778508 1102 46-47 0.6129208390858594 1102 48-49 0.63283036595071 1102 50-51 0.5582811530072149 1102 52-53 0.7447668262118015 1102 54-55 0.901201097662252 1102 56-57 0.9800939414211385 1102 58-59 0.9354256720953273 1102 60-61 0.8572756834099522 1102 62-63 1.0921396470265279 1102 64-65 1.045172638035389 1102 66-67 0.8323258185843514 1102 68-69 0.9827204910232226 1102 70-71 0.8841048169430508 1102 72-73 0.8209087458891915 1102 74-75 0.8109268482492666 1102 76-77 0.8881179799346519 1102 78-79 0.6833684500567792 1102 80-81 0.6028769751003864 1102 82-83 0.8502129641266976 1102 84-85 0.6267440454051929 1102 86-87 0.7469781948611072 1102 88-89 0.9789664843414876 1102 90-91 1.088731920338823 1102 92-93 0.9496698082676751 1102 94-95 0.9454166057031976 1102 96-97 0.8758056400039536 1102 98-99 0.839982260488533 1102 100-101 0.9973460096290516 1103 1 0.276808120306157 1103 2 0.22929486836502022 1103 3 0.22244750328860974 1103 4 0.37724938384818074 1103 5 0.333100337613196 1103 6 0.18136037833845364 1103 7 0.21874162466718872 1103 8 0.2821378198462412 1103 9 0.2670572302303498 1103 10-11 0.22611131021658082 1103 12-13 0.34147029533037454 1103 14-15 0.5590911830241296 1103 16-17 0.44850751936336763 1103 18-19 0.503052283913199 1103 20-21 0.412204344510684 1103 22-23 0.4912896719753945 1103 24-25 0.39551560554934895 1103 26-27 0.6194758895959751 1103 28-29 0.6794382714114704 1103 30-31 0.618867658722408 1103 32-33 0.6890992521502213 1103 34-35 0.5065648525624411 1103 36-37 0.5914315210008638 1103 38-39 0.7839236780779402 1103 40-41 0.8408244638486906 1103 42-43 0.6280512832229022 1103 44-45 0.9648568475287504 1103 46-47 0.7100862750231585 1103 48-49 1.0490224460992579 1103 50-51 0.9806968372495604 1103 52-53 0.9910672969118082 1103 54-55 0.9248354198974269 1103 56-57 1.1074610442410275 1103 58-59 0.978967581669103 1103 60-61 1.0974250982737033 1103 62-63 1.0533677853864205 1103 64-65 0.94088365414283 1103 66-67 0.8538346496073714 1103 68-69 0.9338393751619236 1103 70-71 1.0096478492874894 1103 72-73 1.088392444122988 1103 74-75 1.1682632907936394 1103 76-77 1.1082440757156249 1103 78-79 1.1992176533214547 1103 80-81 1.4139880862115 1103 82-83 1.5356592654610672 1103 84-85 1.3599573286456774 1103 86-87 1.2743029778640889 1103 88-89 1.2621119695079592 1103 90-91 0.9448566342482216 1103 92-93 0.9649852636375442 1103 94-95 1.1925168345756774 1103 96-97 1.0592378635216804 1103 98-99 1.1128915319831165 1103 100-101 1.2108218484682816 1104 1 0.138642802186979 1104 2 0.34451844680315347 1104 3 0.23827951655717428 1104 4 0.2665392218691167 1104 5 0.2756397259357186 1104 6 0.28608478606209786 1104 7 0.2118707149508552 1104 8 0.28256506072465015 1104 9 0.12482545534028588 1104 10-11 0.3192969277549267 1104 12-13 0.25098067728371376 1104 14-15 0.3370245203012274 1104 16-17 0.3373164375237323 1104 18-19 0.20181021799540844 1104 20-21 0.4386220721588643 1104 22-23 0.4449362276470765 1104 24-25 0.4426654706945996 1104 26-27 0.2625632417322947 1104 28-29 0.27057970567005896 1104 30-31 0.2947988795139196 1104 32-33 0.3584202897101676 1104 34-35 0.45295269525892223 1104 36-37 0.31838954403681896 1104 38-39 0.4454820775240549 1104 40-41 0.6767771124134896 1104 42-43 0.4736530027031165 1104 44-45 0.3773173825219658 1104 46-47 0.29526496030438665 1104 48-49 0.5029998395793456 1104 50-51 0.4126943307697388 1104 52-53 0.40748473973273747 1104 54-55 0.4753999256016428 1104 56-57 0.5830568261685158 1104 58-59 0.6649225307617073 1104 60-61 0.4266130995970556 1104 62-63 0.5817431526492527 1104 64-65 0.536157274538219 1104 66-67 0.4169063581545984 1104 68-69 0.303019313683059 1104 70-71 0.4043307817812405 1104 72-73 0.2020691914834032 1104 74-75 0.153723955054609 1104 76-77 0.44254910817497617 1104 78-79 0.6984289009305478 1104 80-81 0.5573777934309945 1104 82-83 0.4665711289543175 1104 84-85 0.5213251129306649 1104 86-87 0.3854809138305413 1104 88-89 0.34438541681601365 1104 90-91 0.3947949581932093 1104 92-93 0.23107416917787305 1104 94-95 0.5351848333574942 1104 96-97 0.8055411344340264 1104 98-99 0.6873968637301786 1104 100-101 0.49760893133329276 1105 1 -0.05534446005272997 1105 2 0.1646542835538618 1105 3 0.1481803974119167 1105 4 0.05732549353035665 1105 5 0.15607764008149871 1105 6 0.24375226799445926 1105 7 0.11554983127639673 1105 8 0.14040233859539342 1105 9 0.08712401422024385 1105 10-11 0.20786604450956503 1105 12-13 0.061045224259572706 1105 14-15 -0.0014318078156279057 1105 16-17 0.2269245583056545 1105 18-19 0.282070980422084 1105 20-21 0.19629200601813324 1105 22-23 0.19649626614233284 1105 24-25 0.20962973998972956 1105 26-27 0.1386612452508018 1105 28-29 0.0025810297105834934 1105 30-31 -0.10268145909718385 1105 32-33 0.2550409214491367 1105 34-35 -0.051690641323212105 1105 36-37 -0.023289535509817938 1105 38-39 0.23021821953966537 1105 40-41 0.08262311914403142 1105 42-43 0.16418458045679074 1105 44-45 -0.16401444287631506 1105 46-47 -0.09749235410006918 1105 48-49 -0.014854785674273785 1105 50-51 0.017164806504858632 1105 52-53 -0.11664068858824805 1105 54-55 0.029031229352476373 1105 56-57 -0.04414914182157759 1105 58-59 -0.21664633467040773 1105 60-61 -0.2746211391102875 1105 62-63 0.06311463796527761 1105 64-65 0.29354523680306244 1105 66-67 0.10641425642415214 1105 68-69 0.29903799736609926 1105 70-71 0.14394533887128702 1105 72-73 0.014948857040575803 1105 74-75 -0.07716787206002351 1105 76-77 -0.05040926423594172 1105 78-79 -0.05561064865047882 1105 80-81 -0.17652377396328234 1105 82-83 -0.1962797264616789 1105 84-85 -0.16648176104642332 1105 86-87 -0.37566769132953937 1105 88-89 -0.13728336463761082 1105 90-91 -0.5953528929993439 1105 92-93 -0.41123873347450157 1105 94-95 -0.2459147945625979 1105 96-97 -0.5932785692506108 1105 98-99 -0.7884930324920134 1105 100-101 -0.3246233029238006 1106 1 -0.0457589842703392 1106 2 0.17723918801650385 1106 3 0.17150928714720592 1106 4 0.18993515518210558 1106 5 0.3447401170700104 1106 6 0.2062594094913166 1106 7 0.2130947433566419 1106 8 0.16842874626377835 1106 9 0.11193674830263944 1106 10-11 0.12245374209097548 1106 12-13 0.33755208009655036 1106 14-15 0.18911126334541706 1106 16-17 0.007621092299181953 1106 18-19 -0.11790045359048662 1106 20-21 0.14390515906834622 1106 22-23 0.02898848984575153 1106 24-25 0.05432598832337732 1106 26-27 0.10493751008925045 1106 28-29 0.2005552251019438 1106 30-31 0.0979747651140741 1106 32-33 0.18681185396992817 1106 34-35 0.014499132053288122 1106 36-37 0.2655205324137526 1106 38-39 0.142346068203409 1106 40-41 0.0785167584508315 1106 42-43 0.3097441564105736 1106 44-45 0.27788154518945163 1106 46-47 0.6662669298458468 1106 48-49 0.5480340291546497 1106 50-51 0.4098995237015828 1106 52-53 0.6668608666710725 1106 54-55 0.6733648483456847 1106 56-57 0.6570307921592473 1106 58-59 0.5010899695256157 1106 60-61 0.40140088844002264 1106 62-63 0.8155308444021472 1106 64-65 0.6584162137598284 1106 66-67 0.5295360427505642 1106 68-69 0.2877068223518151 1106 70-71 0.44036506844661005 1106 72-73 0.35416599028503626 1106 74-75 0.42600612654341674 1106 76-77 0.5924960553857197 1106 78-79 0.5521505714345807 1106 80-81 1.0910125683207283 1106 82-83 1.1353674201135746 1106 84-85 0.9299165440383241 1106 86-87 1.065510179552195 1106 88-89 1.095751612826998 1106 90-91 1.1072037628757272 1106 92-93 1.0422859919746195 1106 94-95 0.6948425215041816 1106 96-97 0.6346383354659544 1106 98-99 0.5248004671588475 1106 100-101 0.6598637789018831 1107 1 0.001446052886009852 1107 2 0.2386020755956686 1107 3 0.02760622312114336 1107 4 0.09477463243070616 1107 5 0.2847774564557355 1107 6 0.03900550166834904 1107 7 -0.16024877899208434 1107 8 0.18440780692197478 1107 9 -0.07083862966712218 1107 10-11 0.05370534365776081 1107 12-13 0.18345161152387135 1107 14-15 0.13484560459811945 1107 16-17 0.10127843022062422 1107 18-19 -0.05851435702059149 1107 20-21 0.4199878182183312 1107 22-23 0.08331363535953074 1107 24-25 0.35120156031152305 1107 26-27 0.21233400311557205 1107 28-29 0.20696378021414574 1107 30-31 0.07370645234180984 1107 32-33 -0.010791744521853275 1107 34-35 0.10920435224584679 1107 36-37 0.07988063784259936 1107 38-39 0.22113400245680737 1107 40-41 0.06363362706743914 1107 42-43 0.009390896535769855 1107 44-45 0.2454406658835211 1107 46-47 0.272729938228899 1107 48-49 0.11133938845472358 1107 50-51 -0.061134996926597296 1107 52-53 0.30059626953375584 1107 54-55 0.151302121838377 1107 56-57 0.6432138466674289 1107 58-59 0.48232875855809 1107 60-61 0.2736736489920588 1107 62-63 0.3977301159180513 1107 64-65 0.6426728122150038 1107 66-67 0.8041658520268342 1107 68-69 0.6145911800777739 1107 70-71 0.7176100074955514 1107 72-73 0.5636440625477306 1107 74-75 0.47046562063135156 1107 76-77 0.7706749366684384 1107 78-79 0.7375090478412147 1107 80-81 0.3050368023142127 1107 82-83 0.589711326838323 1107 84-85 0.604036597484896 1107 86-87 0.530719572970213 1107 88-89 0.33186831671102723 1107 90-91 0.1576805721886103 1107 92-93 -0.06209497958155552 1107 94-95 -0.031737996122206624 1107 96-97 -0.29949151041756394 1107 98-99 -0.4701591733580486 1107 100-101 -0.3465653677833309 1108 1 -0.44102839990403453 1108 2 -0.21621424698765068 1108 3 -0.2198390558834049 1108 4 0.11810905565552332 1108 5 0.12244963244711471 1108 6 -0.05418022044616322 1108 7 -0.1265342906500848 1108 8 0.18008354731903609 1108 9 0.13992182292950162 1108 10-11 -0.2871628239890427 1108 12-13 -0.10761388788436221 1108 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-1.6483119420213157 2316 80-81 -1.823524021163621 2316 82-83 -1.1934089376127481 2316 84-85 -1.226568934147295 2316 86-87 -1.3948793554542434 2316 88-89 -1.5565374725941972 2316 90-91 -1.43558334901401 2316 92-93 -1.7040545319082767 2316 94-95 -1.512346620906694 2316 96-97 -1.630450737290868 2316 98-99 -1.7146419310512542 2316 100-101 -1.7273942788054448 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1002.0 3 324.0 4 293.0 5 343.0 6 427.0 7 463.0 8 587.0 9 783.0 10 1117.0 11 1545.0 12 2304.0 13 3000.0 14 3603.0 15 4944.0 16 6673.0 17 9651.0 18 8386.0 19 4318.0 20 3798.0 21 3749.0 22 4034.0 23 4234.0 24 4765.0 25 5289.0 26 5915.0 27 6765.0 28 7961.0 29 9796.0 30 11963.0 31 14820.0 32 19025.0 33 25037.0 34 34719.0 35 51600.0 36 75380.0 37 112233.0 38 110354.0 39 21932.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.871271504776935 20.160889178573267 15.317747551499975 19.650091765149828 2 14.864118597384243 22.554803612527913 41.0789563046021 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32.5093042001818 18.651705627111667 22-23 20.800416397628865 27.691385559680086 32.45740627837759 19.050791764313455 24-25 20.225436841757777 27.88318989872834 32.80023733804122 19.091135921472663 26-27 19.980707974465933 28.06338073455459 32.71747471672876 19.23843657425072 28-29 19.64481394403659 28.32631089755132 32.72086375102678 19.308011407385305 30-31 19.78597016512245 28.210404652032178 32.61378426903864 19.389840913806733 32-33 19.73114471849765 28.375824631863072 32.42177251049922 19.47125813914007 34-35 19.964331188345902 28.45179351530715 32.238346544798226 19.34552875154872 36-37 20.220254723112514 28.16496125307172 32.24586422198329 19.368919801832483 38-39 19.94632529784744 28.4696279211342 32.203106444822666 19.3809403361957 40-41 19.8675967987269 28.5031287351473 32.07043555354347 19.558838912582335 42-43 19.825154378943072 28.813515724273465 31.50256455106209 19.858765345721373 44-45 19.993655067157682 29.0086899857239 31.12189559155781 19.875759355560604 46-47 20.30411065553389 29.29993157767477 30.52604592698532 19.86991183980602 48-49 20.519653564290476 29.565360544707648 29.773029906274413 20.14195598472747 50-51 20.358471249762065 29.823645315128537 29.665965294961953 20.151918140147444 52-53 20.463797839977225 30.12911091981027 29.293722626735857 20.11336861347665 54-55 20.24954920116646 29.96039486805292 29.25943805128003 20.530617879500593 56-57 20.394038491548354 30.350549867013292 28.87972205692963 20.37568958450872 58-59 20.655916781276474 29.776142182467957 29.009256813995997 20.55868422225957 60-61 20.43438184616572 29.808661056115515 29.59026970945462 20.166687388264144 62-63 20.304253843258337 29.76755671748139 29.755123844278693 20.17306559498158 64-65 20.100078969097446 29.99256610125022 29.624986817048065 20.282368112604274 66-67 20.124976096413562 30.148961147989567 29.184070907255798 20.54199184834107 68-69 20.094794370651652 29.95121357204295 29.380766536060708 20.57322552124469 70-71 20.25299844931454 30.129183763088196 29.096108191187163 20.521709596410094 72-73 20.18405270997934 30.033899933023406 29.01827406204897 20.763773294948283 74-75 19.94072147928725 30.587442250182455 28.82306579384076 20.648770476689528 76-77 20.08544479410309 30.469105814483072 28.830456154435335 20.614993236978503 78-79 20.0361390091188 30.543894660019745 28.593125698403078 20.82684063245839 80-81 19.933705013043674 30.758025871172574 28.391205619975658 20.917063495808094 82-83 20.028271533670658 30.452198720747408 28.531261231201277 20.988268514380657 84-85 20.036945210801242 30.127155673293878 28.568047195191294 21.26785192071359 86-87 20.173927360758206 30.074975195498915 28.431660919683154 21.319436524059725 88-89 20.022930390742776 30.038040094740076 28.45126391559146 21.487765598925684 90-91 19.841060874919915 30.123218131341762 28.80904577347315 21.226675220265168 92-93 19.940936709816206 30.571642758871505 28.39232228691413 21.095098244398162 94-95 19.833829837225615 30.620312583072216 28.04518099667797 21.500676583024198 96-97 20.02920967504022 30.647003735682453 27.792156782030304 21.53162980724702 98-99 20.24603704192905 30.551186099927786 27.60767872979496 21.59509812834821 100-101 20.34625536225743 30.310854384205694 27.63549430556354 21.707395947973342 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1325.0 1 873.5 2 443.0 3 494.5 4 526.0 5 621.5 6 871.5 7 1470.0 8 2268.5 9 2623.0 10 2339.5 11 2050.5 12 2061.5 13 2087.5 14 2132.0 15 2205.5 16 2196.0 17 2243.0 18 2357.0 19 2387.0 20 2545.0 21 2875.5 22 3216.5 23 3607.5 24 4210.0 25 5020.0 26 5925.5 27 6669.0 28 7375.0 29 8178.0 30 9446.5 31 11005.5 32 12399.0 33 13772.0 34 15305.5 35 16318.0 36 17497.5 37 19059.0 38 19174.5 39 18525.0 40 18148.5 41 18417.5 42 18584.5 43 18711.0 44 19459.5 45 19510.5 46 18862.5 47 19011.0 48 21027.5 49 20568.0 50 17592.5 51 15976.0 52 14922.0 53 14580.0 54 14033.0 55 12563.0 56 11092.5 57 9826.5 58 8864.5 59 7908.0 60 6860.0 61 5806.0 62 4585.5 63 3304.0 64 2291.5 65 1740.0 66 1226.5 67 871.5 68 577.0 69 337.5 70 230.0 71 164.0 72 118.5 73 94.5 74 76.5 75 53.5 76 36.5 77 20.5 78 11.5 79 7.0 80 9.0 81 8.0 82 1.5 83 1.5 84 2.5 85 2.0 86 1.5 87 1.5 88 2.0 89 1.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022807441742419953 2 0.010460555987124942 3 0.011489463133399528 4 0.02383634888869454 5 0.06104849067895868 6 0.0463008215823563 7 0.018005875059805228 8 0.056589893045102146 9 0.03052424533947934 10-11 0.026751585803139195 12-13 0.03987015191814015 14-15 0.020578142925491687 16-17 0.01731993696228884 18-19 0.01774864827323658 20-21 0.012518370279674111 22-23 0.007974030383628029 24-25 0.0014576184572223279 26-27 8.574226218954871E-5 28-29 0.0020578142925491687 30-31 0.0 32-33 6.859380975163897E-4 34-35 8.574226218954871E-5 36-37 0.0 38-39 8.574226218954871E-5 40-41 0.0 42-43 0.0 44-45 8.574226218954871E-5 46-47 0.0 48-49 7.716803597059383E-4 50-51 0.0 52-53 1.7148452437909742E-4 54-55 0.0019720720303596203 56-57 3.4296904875819484E-4 58-59 8.574226218954871E-4 60-61 0.00265801012787601 62-63 0.0019720720303596203 64-65 6.001958353268409E-4 66-67 0.012261143493105465 68-69 0.015690833980687414 70-71 0.030009791766342043 72-73 0.01774864827323658 74-75 0.022721699480230406 76-77 0.03318225546735534 78-79 0.019977947090164847 80-81 0.05033070790526509 82-83 0.038498275723107364 84-85 0.020149431614543946 86-87 0.014318957785654634 88-89 0.012689854804053208 90-91 0.026151389967812355 92-93 0.03584026559523136 94-95 0.011317978609020429 96-97 0.0200636893523544 98-99 0.03121018343699573 100-101 0.04278538883258481 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 583143.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.61265509278044 #Duplication Level Percentage of deduplicated Percentage of total 1 81.13593150594731 49.17864232001443 2 11.0070592602453 13.34334173054087 3 3.341952318712891 6.0769380969198705 4 1.4253992490404535 3.45588932206389 5 0.7292655225004347 2.2101359793187583 6 0.48743176704752467 1.772672014638967 7 0.31537217544231416 1.338088142716138 8 0.24291517242976926 1.1778986850631115 9 0.18620115356564693 1.0157531669057163 >10 1.0303849301232948 11.466454509795971 >50 0.0708720767583235 2.910968263959521 >100 0.024946962504508806 2.6490989647506837 >500 8.504646308351402E-4 0.4311207953919493 >1k 0.0011339528411135203 1.8616033070053322 >5k 2.834882102783801E-4 1.111394700914758 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6468 1.1091619036840021 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4636 0.7950022550214956 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2985 0.5118813052716058 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2147 0.3681772738419221 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1066 0.18280250298811784 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 898 0.15399310289242946 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 881 0.1510778659779848 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 730 0.12518370279674113 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.57422621895487E-5 0.0 18-19 0.0 0.0 0.0 3.429690487581948E-4 0.0 20-21 0.0 0.0 0.0 6.001958353268409E-4 0.0 22-23 0.0 0.0 0.0 0.0012003916706536818 0.0 24-25 0.0 0.0 0.0 0.0020578142925491687 0.0 26-27 0.0 0.0 0.0 0.0028294946522551072 0.0 28-29 0.0 0.0 0.0 0.006001958353268409 0.0 30-31 0.0 0.0 0.0 0.011317978609020429 0.0 32-33 0.0 0.0 0.0 0.015948060767256057 0.0 34-35 0.0 0.0 0.0 0.020149431614543946 0.0 36-37 0.0 0.0 0.0 0.026837328065328743 0.0 38-39 0.0 0.0 0.0 0.038069564412159626 0.0 40-41 0.0 0.0 0.0 0.05581821268539621 0.0 42-43 0.0 0.0 0.0 0.09174422054281711 0.0 44-45 0.0 0.0 0.0 0.12346885755295013 0.0 46-47 0.0 0.0 0.0 0.20132283162106035 0.0 48-49 0.0 0.0 0.0 0.27214594018962757 0.0 50-51 0.0 0.0 0.0 0.32127625642423896 0.0 52-53 0.0 0.0 0.0 0.3921851072549958 0.0 54-55 0.0 0.0 0.0 0.4634369271345108 0.0 56-57 0.0 0.0 0.0 0.6310630497150784 0.0 58-59 0.0 0.0 0.0 0.8069204294658429 0.0 60-61 0.0 0.0 0.0 1.0097008795441256 0.0 62-63 0.0 0.0 0.0 1.1973049492148582 0.0 64-65 0.0 0.0 0.0 1.3978561004762124 0.0 66-67 0.0 0.0 0.0 1.7071284401939146 0.0 68-69 0.0 0.0 0.0 2.1030862069852505 0.0 70-71 0.0 0.0 0.0 2.3748034358639307 0.0 72-73 0.0 0.0 0.0 2.623970449786759 0.0 74-75 0.0 0.0 0.0 2.932556851406945 0.0 76-77 0.0 0.0 0.0 3.271067302531283 0.0 78-79 0.0 0.0 0.0 3.5979168059978424 0.0 80-81 0.0 0.0 0.0 4.137578604218862 0.0 82-83 0.0 0.0 0.0 4.784161003390249 0.0 84-85 0.0 0.0 0.0 5.5297757153905645 0.0 86-87 0.0 0.0 0.0 6.169155764538029 0.0 88-89 0.0 1.714845243790974E-4 0.0 6.919915012269717 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGG 50 0.0016117397 38.01641 1 GTATCAA 6330 0.0 36.71016 1 GGTATCA 3895 0.0 34.405094 1 ATCAACG 7770 0.0 29.845573 3 TATCAAC 7815 0.0 29.734524 2 TCAACGC 7875 0.0 29.387285 4 CAACGCA 7950 0.0 29.050274 5 AACGCAG 8095 0.0 28.532368 6 GTCGGGA 120 5.357684E-6 27.720299 2 GCGTCAG 70 0.008370587 27.15458 1 GTGTAGG 175 4.9276423E-9 27.154575 1 CGGCAGC 90 8.915251E-4 26.40255 6 GAGTACT 4100 0.0 26.248804 12-13 GTACATG 4780 0.0 26.046808 1 ACGCAGA 8780 0.0 26.03345 7 CGCAGAG 8745 0.0 25.86594 8 TACATGG 4845 0.0 25.108877 2 ACATGGG 4825 0.0 24.819004 3 GCAGAGT 8875 0.0 24.683893 9 GTACTTT 4475 0.0 24.150183 14-15 >>END_MODULE