FastQCFastQC Report
Thu 26 May 2016
SRR936833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936833_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583143
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100771.7280495521681645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76771.3164866936583308No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51140.8769718576747042No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19810.339710842794992No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16340.28020571283544515No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14380.2465947460571421No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13880.2380205198381872No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8170.14010285641772258No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7200.12346885755295013No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7010.12021065158974728No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.11969619801660998No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5880.10083290033490928No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46400.044.846461
GTATCAA76500.037.2616271
CCGTTGC902.4093762E-531.669679
CATATAG1102.7338665E-630.222363
ATCAACG94650.030.005693
TATCAAC95100.029.9136472
TCAACGC95550.029.6733574
CAACGCA96800.029.248645
AACGCAG98300.028.8023246
ACGCAGA109150.025.85227
CGCAGAG109150.025.7651568
GAGTACT67150.025.63805412-13
GCAGAGT111100.024.9281089
TATTCCG1151.2798703E-424.7849585
TTCAACG1201.7112927E-423.746142
GTACTTT72300.023.71329714-15
AGTACTT68550.023.1745712-13
GTACATG43350.023.0145361
GGATAAG2503.8016879E-1022.804121
AGAGTAC95150.022.29191810-11
Produced by FastQC (version 0.11.5)