##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936833_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 583143 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20302738779339 33.0 31.0 34.0 31.0 34.0 2 32.294303455584654 33.0 31.0 34.0 31.0 34.0 3 32.363183644492004 34.0 31.0 34.0 30.0 34.0 4 36.058970098243485 37.0 35.0 37.0 35.0 37.0 5 35.993747674241135 37.0 35.0 37.0 35.0 37.0 6 36.05733070619042 37.0 35.0 37.0 35.0 37.0 7 36.006585005736156 37.0 35.0 37.0 35.0 37.0 8 35.978597016512246 37.0 35.0 37.0 35.0 37.0 9 37.6396321313983 39.0 37.0 39.0 35.0 39.0 10-11 37.571109487724286 39.0 37.0 39.0 35.0 39.0 12-13 37.46858489255637 39.0 37.0 39.0 35.0 39.0 14-15 38.58402998921362 40.0 38.0 41.0 34.0 41.0 16-17 38.5281440401411 40.0 38.0 41.0 34.0 41.0 18-19 38.76904892967934 40.0 38.0 41.0 35.0 41.0 20-21 38.84096182240034 40.0 38.0 41.0 35.0 41.0 22-23 38.745611282309824 40.0 38.0 41.0 35.0 41.0 24-25 38.626330076842216 40.0 38.0 41.0 34.0 41.0 26-27 38.419968172472274 40.0 38.0 41.0 34.0 41.0 28-29 38.291059825806016 40.0 38.0 41.0 34.0 41.0 30-31 38.10066656034626 40.0 37.0 41.0 34.0 41.0 32-33 37.751069634720814 40.0 36.0 41.0 33.0 41.0 34-35 37.51566253903417 40.0 35.0 41.0 33.0 41.0 36-37 37.29056252068532 40.0 35.0 41.0 33.0 41.0 38-39 37.07117465184355 39.5 35.0 41.0 33.0 41.0 40-41 36.80630565744595 39.0 35.0 41.0 31.5 41.0 42-43 36.408669057160935 39.0 35.0 41.0 31.0 41.0 44-45 35.89537557683107 39.0 35.0 41.0 29.5 41.0 46-47 35.168709904774644 39.0 35.0 41.0 25.5 41.0 48-49 34.48278895571069 39.0 35.0 40.0 21.5 41.0 50-51 34.08422633899404 38.5 34.0 40.0 16.5 41.0 52-53 33.816509844069124 38.0 34.0 40.0 13.0 41.0 54-55 33.518007589905054 38.0 34.0 40.0 8.0 41.0 56-57 33.23028056583034 38.0 33.0 40.0 7.0 41.0 58-59 32.890303922022554 37.5 33.0 40.0 3.5 41.0 60-61 32.563411547424906 37.0 33.0 40.0 2.0 41.0 62-63 32.20997165360812 36.5 32.0 39.5 2.0 41.0 64-65 31.834980442189995 36.0 31.5 39.0 2.0 40.5 66-67 31.456864782737682 35.5 31.0 39.0 2.0 40.0 68-69 31.164753756797218 35.0 31.0 39.0 2.0 40.0 70-71 30.711546738964543 35.0 30.5 38.0 2.0 40.0 72-73 30.213036082058775 35.0 30.0 37.5 2.0 39.5 74-75 29.71205261831146 34.5 29.0 37.0 2.0 39.0 76-77 28.31812694313402 33.0 27.0 35.0 2.0 37.5 78-79 28.99723481204439 34.0 29.0 36.0 2.0 38.0 80-81 28.72936912558326 34.0 29.0 35.5 2.0 37.5 82-83 28.41024928705309 34.0 29.0 35.0 2.0 37.0 84-85 28.07506306343384 34.0 28.0 35.0 2.0 36.5 86-87 27.74477872494397 34.0 27.0 35.0 2.0 36.0 88-89 27.349946754055182 34.0 26.5 35.0 2.0 36.0 90-91 26.905707519424908 33.0 26.0 35.0 2.0 35.0 92-93 26.457287492090273 33.0 25.0 35.0 2.0 35.0 94-95 25.89157633719345 33.0 23.5 35.0 2.0 35.0 96-97 25.272985356936466 32.0 19.5 34.0 2.0 35.0 98-99 24.53178637143891 32.0 9.0 34.0 2.0 35.0 100-101 21.40054240555061 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0694719103411856 1101 2 0.13022294647407762 1101 3 0.04621827161201253 1101 4 0.08036199465577454 1101 5 0.08700209087621147 1101 6 0.08901814010999232 1101 7 0.12022531326326913 1101 8 0.09938635894751258 1101 9 0.19737378097345015 1101 10-11 0.21450958056397695 1101 12-13 0.17076632369349198 1101 14-15 0.16619829003777653 1101 16-17 0.161260504933054 1101 18-19 0.16400072888984596 1101 20-21 0.22087920918306025 1101 22-23 0.16950381031674056 1101 24-25 0.10557456460519887 1101 26-27 0.06688127795850107 1101 28-29 0.07714448457404188 1101 30-31 0.20738233170283848 1101 32-33 0.3001206188555017 1101 34-35 0.3733048950140798 1101 36-37 0.14660197764558092 1101 38-39 0.2065986863238436 1101 40-41 0.143493137906205 1101 42-43 -0.027120219361250975 1101 44-45 0.23834281281702374 1101 46-47 0.19260415186067092 1101 48-49 0.2786502157217754 1101 50-51 0.33794460389641046 1101 52-53 0.343193042385451 1101 54-55 0.1944080626167377 1101 56-57 0.2680533501332647 1101 58-59 0.27491692262515954 1101 60-61 0.3247088490131489 1101 62-63 0.30828899587560343 1101 64-65 0.2915096791002618 1101 66-67 0.3145135941231132 1101 68-69 0.29287934664267823 1101 70-71 0.41268660129757606 1101 72-73 0.4134243732625329 1101 74-75 0.17806498518203284 1101 76-77 0.16300271346132789 1101 78-79 0.03329910252903545 1101 80-81 0.16616177666783472 1101 82-83 0.31072115809996603 1101 84-85 0.1533486379508915 1101 86-87 -0.20609507760533674 1101 88-89 -0.1385703064330137 1101 90-91 -0.025571835778212204 1101 92-93 -0.051683647795400134 1101 94-95 -0.3702371361128449 1101 96-97 -0.3399375755410112 1101 98-99 0.045626569089833424 1101 100-101 0.6277737274005766 1102 1 0.29354721265585937 1102 2 0.2852693404713307 1102 3 0.21737560923302368 1102 4 0.13721390233673958 1102 5 0.27168302746549955 1102 6 0.28327667359071285 1102 7 0.09915406414257433 1102 8 0.2270412010069407 1102 9 0.21261513025157797 1102 10-11 0.30934963014023964 1102 12-13 0.365950057321605 1102 14-15 0.2340929739891422 1102 16-17 0.41484305945019173 1102 18-19 0.37917575317551666 1102 20-21 0.22787080131785586 1102 22-23 0.27397304288967916 1102 24-25 0.3123202853032936 1102 26-27 0.3273951291627384 1102 28-29 0.3455128227645048 1102 30-31 0.4437773338801705 1102 32-33 0.40378055253050604 1102 34-35 0.32575456506398837 1102 36-37 0.17405248513226468 1102 38-39 0.22954780366548277 1102 40-41 0.43205174173161254 1102 42-43 0.4137654537704307 1102 44-45 0.38473011057604367 1102 46-47 0.5182444560177117 1102 48-49 0.49478192777751673 1102 50-51 0.7005620962757391 1102 52-53 0.7678887178619576 1102 54-55 0.6706141390579674 1102 56-57 0.5273197555196631 1102 58-59 0.7106908816912423 1102 60-61 0.7900302572468263 1102 62-63 0.8235223057607008 1102 64-65 0.6183592419582631 1102 66-67 0.7021503475482334 1102 68-69 0.5846322692970212 1102 70-71 0.3728761969830998 1102 72-73 0.6076842466420906 1102 74-75 0.5057178173931973 1102 76-77 0.4605801763628783 1102 78-79 0.3516403077680401 1102 80-81 0.4079800193905072 1102 82-83 0.5529855871355736 1102 84-85 0.5255732049960784 1102 86-87 0.1499455213294425 1102 88-89 0.3609386206429903 1102 90-91 0.8887945595589422 1102 92-93 0.6491370402426497 1102 94-95 0.5179456260886077 1102 96-97 0.9188116288940513 1102 98-99 1.2675868410892939 1102 100-101 1.5816858970317682 1103 1 0.1947831240433473 1103 2 0.2083422773802539 1103 3 0.12428755810377368 1103 4 0.08885703400319045 1103 5 0.14829398743440692 1103 6 0.03346884010623796 1103 7 0.04645984641143741 1103 8 -0.010974085947331957 1103 9 0.08742620249755362 1103 10-11 0.11250029732510569 1103 12-13 0.13731163265519086 1103 14-15 0.19242781874450543 1103 16-17 0.16076006078887417 1103 18-19 0.25578844048388305 1103 20-21 0.1352344494588067 1103 22-23 0.04504960721148166 1103 24-25 0.21152484547948092 1103 26-27 0.33219799654624893 1103 28-29 0.18265062127036202 1103 30-31 0.05459047893351254 1103 32-33 0.04964832469443081 1103 34-35 0.167503496272694 1103 36-37 0.06153272868713344 1103 38-39 -0.064829706374681 1103 40-41 0.03342670394606273 1103 42-43 0.2843687270787143 1103 44-45 0.14081250466853845 1103 46-47 0.2955705345252895 1103 48-49 0.39123138150641523 1103 50-51 0.33623683825440054 1103 52-53 0.37140558101349086 1103 54-55 0.4383120273854644 1103 56-57 0.4993273558133069 1103 58-59 0.447269885880047 1103 60-61 0.42476014453792743 1103 62-63 0.7601963136201029 1103 64-65 0.3602144045440916 1103 66-67 0.20343376077101283 1103 68-69 0.25974994429809684 1103 70-71 0.17951090786965906 1103 72-73 0.1842459774362375 1103 74-75 0.40244537277602177 1103 76-77 0.3193477196543242 1103 78-79 0.45532990486610814 1103 80-81 0.3078375138846141 1103 82-83 0.4705880399576152 1103 84-85 0.3561911606898214 1103 86-87 0.36453635779357896 1103 88-89 0.6691840755810254 1103 90-91 0.5294207201151444 1103 92-93 0.36555540186116886 1103 94-95 0.14300586705246232 1103 96-97 0.5453668821652009 1103 98-99 0.4693651370689125 1103 100-101 0.5202720696793506 1104 1 0.1355281006765594 1104 2 0.12322932146494026 1104 3 0.07428284749921943 1104 4 -0.020613033939063996 1104 5 0.05563530031276542 1104 6 -0.0865770828107415 1104 7 0.09988686600097907 1104 8 0.07396797361662522 1104 9 0.020386631228298313 1104 10-11 0.2443731241479412 1104 12-13 -0.06040229999849345 1104 14-15 -0.1511253354982145 1104 16-17 -0.02934679325030487 1104 18-19 -0.00637094446452835 1104 20-21 0.2681622326044817 1104 22-23 0.12673477669464717 1104 24-25 0.19540143202204519 1104 26-27 0.20408379396396015 1104 28-29 0.255617885855159 1104 30-31 0.2985526889300658 1104 32-33 0.3662524389145432 1104 34-35 0.08896347694825835 1104 36-37 0.3055365672820969 1104 38-39 0.35524338468020744 1104 40-41 0.6787926741005563 1104 42-43 0.7707835626347972 1104 44-45 0.40309239345445036 1104 46-47 0.21292900276814208 1104 48-49 0.3244656825951111 1104 50-51 0.11950301808556674 1104 52-53 -0.05689244469546395 1104 54-55 0.07645543571600655 1104 56-57 0.16694490730678524 1104 58-59 0.2546948941509939 1104 60-61 0.2792451878207203 1104 62-63 0.19107312145152733 1104 64-65 0.3418331873676941 1104 66-67 0.30287681394388244 1104 68-69 0.3051908459297188 1104 70-71 -0.0641588064486065 1104 72-73 0.21286454335293215 1104 74-75 0.24083000787944542 1104 76-77 0.19508416242485893 1104 78-79 0.0024940113739759795 1104 80-81 0.27118681945386314 1104 82-83 0.28583812924000185 1104 84-85 0.11052436913735875 1104 86-87 -0.04370106544000052 1104 88-89 0.29164555269515446 1104 90-91 -0.021783551417247082 1104 92-93 -0.014354855644576503 1104 94-95 0.1766362971197033 1104 96-97 0.49807460339381393 1104 98-99 0.5330448422507956 1104 100-101 0.68294348638819 1105 1 0.18271236911351707 1105 2 0.19672276562537405 1105 3 0.15200893048468345 1105 4 0.18773945507324186 1105 5 0.04653865148386416 1105 6 0.23243699737952284 1105 7 0.10795677043712715 1105 8 0.11052313890714771 1105 9 0.3064897224951437 1105 10-11 0.17296688276800154 1105 12-13 0.14955160377562748 1105 14-15 0.1438138873635566 1105 16-17 0.22926253510561878 1105 18-19 0.0270457543836784 1105 20-21 0.22074503111125665 1105 22-23 0.16001508710859724 1105 24-25 -0.03364685254858557 1105 26-27 -0.03515531181830056 1105 28-29 0.11379843349303087 1105 30-31 0.08849764317098163 1105 32-33 0.14084833071100888 1105 34-35 0.36809236861599715 1105 36-37 0.06697020894524286 1105 38-39 0.554828702541883 1105 40-41 0.23426752039538457 1105 42-43 0.5833812565975407 1105 44-45 0.1406320073985583 1105 46-47 0.2231121617081797 1105 48-49 0.5326434718152129 1105 50-51 0.7470185418478081 1105 52-53 0.812988391656809 1105 54-55 0.7467141753029765 1105 56-57 0.5454519290124651 1105 58-59 0.4316719134660403 1105 60-61 0.19368979572695366 1105 62-63 0.5224663676188648 1105 64-65 0.29696139249590203 1105 66-67 0.3546415883359586 1105 68-69 0.37863371909767096 1105 70-71 0.2179154624174373 1105 72-73 0.2615552143840283 1105 74-75 0.3385944635210265 1105 76-77 0.144859504621909 1105 78-79 0.8292638428652985 1105 80-81 0.8226834143403714 1105 82-83 0.7365206843930316 1105 84-85 0.47843293678295495 1105 86-87 -0.1674605436699501 1105 88-89 -0.05362100240883905 1105 90-91 0.23620666539438773 1105 92-93 0.23936900834703678 1105 94-95 0.33393813545190554 1105 96-97 0.4748150117622423 1105 98-99 0.4793338508754488 1105 100-101 0.676646631597432 1106 1 -0.16388034338569213 1106 2 -0.18058631835862826 1106 3 -0.19505194680853322 1106 4 -0.022149137575780742 1106 5 -0.06752692332727861 1106 6 0.047369073809413464 1106 7 -0.056480162210625906 1106 8 0.04123474939403593 1106 9 0.1768715545271391 1106 10-11 -0.1351172916749661 1106 12-13 0.09947384080984989 1106 14-15 0.029073837849175277 1106 16-17 -0.05825016088879664 1106 18-19 -0.08492894884744828 1106 20-21 0.02358020536377836 1106 22-23 -0.2132590511507857 1106 24-25 -0.12798999353760365 1106 26-27 -0.09235473415054685 1106 28-29 -0.06621489730110142 1106 30-31 0.11577022617104404 1106 32-33 0.07927672530252039 1106 34-35 -0.05765986905301901 1106 36-37 -0.17396009004542634 1106 38-39 -0.24481660311418096 1106 40-41 -0.27611939003437413 1106 42-43 -0.2703132777768644 1106 44-45 -0.8810503705611978 1106 46-47 -0.54158741932401 1106 48-49 -0.8718747151967889 1106 50-51 -0.8774299758948487 1106 52-53 -0.9623163122091185 1106 54-55 -1.1029930233258511 1106 56-57 -0.8673781668422933 1106 58-59 -0.5220551543940459 1106 60-61 -0.6326091632015007 1106 62-63 -0.6229265734082396 1106 64-65 -1.0434340877300876 1106 66-67 -0.7058786300351478 1106 68-69 -0.969823717581054 1106 70-71 -1.436634464580596 1106 72-73 -1.2479786839210583 1106 74-75 -1.0357310617757456 1106 76-77 -0.786824142937288 1106 78-79 -0.4951584893888743 1106 80-81 -0.6355546990348593 1106 82-83 -0.35318445113670904 1106 84-85 -0.46508972556707207 1106 86-87 -0.5703401539029223 1106 88-89 -0.8530758681486965 1106 90-91 -0.5914214054492604 1106 92-93 -0.36233780940196425 1106 94-95 -0.1887916620469845 1106 96-97 -0.49397267947117385 1106 98-99 -0.8419620927543363 1106 100-101 0.19136605012088737 1107 1 -0.031842601320306585 1107 2 0.05008505843969857 1107 3 -0.03250664534790104 1107 4 -0.07369670868534683 1107 5 -0.02402379340818328 1107 6 0.0017884829685428372 1107 7 0.05247671445957991 1107 8 0.11898852909165925 1107 9 -0.27188288410081896 1107 10-11 -0.05477354968279968 1107 12-13 -0.03249068677370559 1107 14-15 0.11370977883830591 1107 16-17 -0.016474435150243494 1107 18-19 -0.11504984426056097 1107 20-21 -0.03240370575383622 1107 22-23 -0.04597539994457378 1107 24-25 -0.3033684208905427 1107 26-27 -0.13516461444487504 1107 28-29 0.02097439874165019 1107 30-31 -0.3641095190425574 1107 32-33 -0.1031211404953325 1107 34-35 -0.2724116719596381 1107 36-37 -0.20116384594834358 1107 38-39 -0.36570057646410703 1107 40-41 -0.266432198907971 1107 42-43 -0.4303967422861419 1107 44-45 -0.3209481448409477 1107 46-47 -0.6743870495908766 1107 48-49 -0.537453841748011 1107 50-51 -0.620257244628597 1107 52-53 -0.4070531061469609 1107 54-55 -0.5431516241971011 1107 56-57 -0.4668194488278701 1107 58-59 -0.7862198686745288 1107 60-61 -0.5792472457142246 1107 62-63 -0.4253984773508428 1107 64-65 -0.6791005225508933 1107 66-67 -0.5985965346366768 1107 68-69 -0.6074669434111257 1107 70-71 -0.3075069981677352 1107 72-73 -0.47332599511921103 1107 74-75 -0.7353411946600303 1107 76-77 -0.6394044958310907 1107 78-79 -0.356604658441114 1107 80-81 -0.17005244768954597 1107 82-83 -0.5401879654318371 1107 84-85 -0.5443787214304514 1107 86-87 -0.7260650687910903 1107 88-89 -0.7270806087863981 1107 90-91 -0.8224908859925115 1107 92-93 -0.25541949846005707 1107 94-95 -0.427669924667331 1107 96-97 -0.6045242590736173 1107 98-99 -0.8054417940392504 1107 100-101 -0.6931792879469825 1108 1 0.015318998886002078 1108 2 0.05026005510094933 1108 3 0.09795336561241186 1108 4 0.020589650324907893 1108 5 0.10457112155725667 1108 6 -0.1566848093173263 1108 7 0.12009910850601102 1108 8 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0.02068106947550774 2316 88-89 -0.21348149746249945 2316 90-91 -0.1872300256658228 2316 92-93 -0.36476646676735314 2316 94-95 -0.1675089193659396 2316 96-97 -0.1491046527915607 2316 98-99 -0.7158856210486562 2316 100-101 -0.7945580269629282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 2.0 5 18.0 6 50.0 7 98.0 8 212.0 9 445.0 10 851.0 11 1507.0 12 2387.0 13 3272.0 14 4266.0 15 6151.0 16 9021.0 17 13257.0 18 11839.0 19 5737.0 20 4195.0 21 4055.0 22 4083.0 23 4301.0 24 4807.0 25 5383.0 26 6253.0 27 7417.0 28 9084.0 29 11019.0 30 13317.0 31 16674.0 32 21235.0 33 27227.0 34 37892.0 35 54530.0 36 78410.0 37 118363.0 38 89523.0 39 6259.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.51553695333339 19.82503237758699 16.282624171262423 19.376806497817192 2 15.43142019759599 22.23272149803896 40.94071605823268 21.39514224613237 3 18.216972509315895 28.209890198459043 32.57142759151701 21.00170970070806 4 11.641761354867254 18.85132609210107 39.098538606377176 30.408373946654503 5 11.507469008459331 38.94636478530995 34.10038361088104 15.445782595349684 6 27.338062876515707 39.071548488106686 17.900755046360842 15.689633589016761 7 24.432600579960663 37.247639086810615 21.498671852358683 16.821088480870046 8 26.216211118027655 33.33333333333333 20.227285588612055 20.223169960026958 9 27.42157583988833 16.249359076590135 21.339191244686123 34.989873838835415 10-11 23.654412798814516 28.2262877880951 26.928294806312962 21.191004606777415 12-13 26.399699559113287 26.7778229353692 28.208947033574955 18.61353047194256 14-15 23.044776324160626 26.16107884343977 29.641700234762315 21.152444597637285 16-17 18.98719525056461 30.063294937948328 29.575592950614173 21.373916860872892 18-19 18.82735050356603 26.31216120972458 35.89620692079617 18.96428136591322 20-21 20.495735539117543 24.902187920947778 36.19539950971776 18.406677030216922 22-23 19.862294857064462 25.388592595133158 36.16474885531528 18.584363692487095 24-25 19.500014149086784 25.40164112256282 36.47222963024577 18.626115098104624 26-27 19.317323382205785 25.580222819968224 36.53695273585982 18.565501061966177 28-29 18.815105450573235 25.930457582692334 36.47362856807236 18.78080839866207 30-31 19.09708110184504 25.73649831684476 36.43340861658872 18.733011964721477 32-33 19.158581601636023 25.687484190714642 36.182534544628275 18.971399663021064 34-35 19.271773818771727 25.821282258382595 36.00926359400696 18.897680328838725 36-37 19.47087887305586 25.522424150958926 36.02329964551474 18.98339733047047 38-39 19.247626536823304 25.975647508881995 35.97227736696729 18.804448587327407 40-41 19.070532744681596 26.128437314849506 35.698000739101474 19.103029201367423 42-43 19.26345681933934 26.119836815326604 35.18562342341415 19.431082941919904 44-45 19.449946239601605 26.36660304590812 34.59991803039735 19.583532684092926 46-47 19.79204000404705 26.885521683435055 33.72100401103678 19.601434301481117 48-49 20.189136768743474 27.23473435278663 32.600326174752155 19.97580270371774 50-51 19.930886936687802 27.459065944666676 32.38615852272971 20.223888595915813 52-53 20.058544816623026 27.931399330867386 31.75704758524067 20.25300826726892 54-55 19.791998038137983 27.78830068279315 31.71562604234638 20.704075236722492 56-57 20.139319403107933 28.125576103052456 31.26060565526336 20.474498838576245 58-59 20.436773330589567 27.478564324176013 31.51524505264602 20.5694172925884 60-61 20.225420466189075 27.38399934834707 32.180164885339096 20.210415300124758 62-63 20.04560008094486 27.51293488569111 32.30565945194156 20.13580558142247 64-65 19.957814770832172 27.71672447129132 32.08872616898957 20.236734588886936 66-67 20.012844356071614 27.894888923757854 31.55844567857728 20.53382104159325 68-69 20.215642898789312 27.489196419384708 31.824004527214733 20.471156154611243 70-71 20.171589180587066 27.775891428600826 31.49482883096082 20.557690559851288 72-73 20.24889263868382 27.667055936537004 31.383640033405186 20.700411391373986 74-75 19.837415522436178 28.10219791714897 31.161910543383016 20.898476017031843 76-77 19.760333228727774 28.079904928979683 31.22887525015305 20.93088659213949 78-79 19.594330991682035 28.127767903000244 31.136416836224175 21.141484269093546 80-81 19.621493191524753 28.210092543131204 30.733785659716002 21.434628605628042 82-83 19.673694374460833 27.885016112905248 30.93317646332449 21.508113049309436 84-85 19.368807370916777 27.74443601253639 30.916091081756637 21.970665534790196 86-87 19.301643811419993 27.768160081903325 30.677991825101415 22.252204281575267 88-89 19.244798008032348 27.540633327731495 30.576086099097648 22.63848256513851 90-91 19.12838888188655 27.54877271865946 31.008756924812886 22.3140814746411 92-93 18.830437235591393 27.603444106999852 30.982643991761854 22.583474665646904 94-95 18.648084603604946 27.695865336632696 30.65903217564131 22.99701788412105 96-97 18.89384681605614 27.612329347365677 30.47456051748412 23.019263319094062 98-99 18.80154783922433 27.49841160416404 30.31971128494149 23.380329271670135 100-101 18.49212881983743 27.00209212196042 30.25268374661316 24.253095311588986 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1456.0 1 1042.0 2 654.5 3 665.5 4 549.5 5 526.5 6 919.5 7 2006.5 8 3376.5 9 3882.0 10 3104.0 11 2244.0 12 2109.0 13 2179.0 14 2273.0 15 2401.5 16 2426.0 17 2454.5 18 2587.5 19 2678.5 20 2857.0 21 3154.0 22 3426.0 23 3853.5 24 4447.0 25 5101.0 26 5865.0 27 6706.0 28 7367.5 29 7999.5 30 9129.5 31 10499.0 32 11881.5 33 13139.0 34 14661.0 35 15812.5 36 16892.0 37 18376.5 38 18640.5 39 18151.5 40 18163.0 41 18420.0 42 18474.5 43 18699.5 44 19057.0 45 19275.0 46 19003.0 47 19208.0 48 21140.0 49 20432.0 50 17223.0 51 15774.0 52 15046.5 53 14717.5 54 13952.5 55 12364.0 56 10933.5 57 9616.0 58 8704.0 59 7893.0 60 6831.0 61 5580.0 62 4478.5 63 3473.5 64 2354.5 65 1605.0 66 1173.5 67 906.5 68 614.0 69 338.5 70 235.0 71 165.0 72 118.5 73 103.0 74 87.0 75 84.0 76 66.0 77 32.5 78 13.5 79 6.5 80 4.0 81 2.5 82 2.5 83 2.5 84 1.5 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03052424533947934 2 0.0058304738288893114 3 0.0 4 1.7148452437909742E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01526212266973967 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.4296904875819484E-4 20-21 0.0016291029816014255 22-23 0.0022292988169282666 24-25 0.011403720871209977 26-27 0.004544339896046081 28-29 4.2871131094774354E-4 30-31 0.0020578142925491687 32-33 0.0035154327497714973 34-35 0.0 36-37 0.007545319072680286 38-39 0.013975988736896438 40-41 4.2871131094774354E-4 42-43 0.0 44-45 0.0 46-47 3.4296904875819484E-4 48-49 0.0034296904875819483 50-51 0.006945123237353444 52-53 0.0 54-55 0.004029886322908789 56-57 0.0038584017985296916 58-59 5.144535731372922E-4 60-61 0.0018005875059805226 62-63 0.005144535731372922 64-65 8.574226218954871E-5 66-67 0.0012861339328432305 68-69 5.144535731372922E-4 70-71 0.0010289071462745844 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0012861339328432305 80-81 0.0016291029816014255 82-83 0.012003916706536819 84-85 0.006945123237353444 86-87 0.0028294946522551072 88-89 1.7148452437909742E-4 90-91 7.716803597059383E-4 92-93 3.4296904875819484E-4 94-95 0.0 96-97 0.0024007833413073635 98-99 0.0012861339328432305 100-101 5.144535731372922E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 583143.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.1760881910492 #Duplication Level Percentage of deduplicated Percentage of total 1 79.60547332919448 44.719240902310446 2 11.598568816027829 13.03124449398265 3 3.619772555734181 6.1003398696738875 4 1.6166333075242005 3.6326454102426826 5 0.8657809511402172 2.4318093532691654 6 0.5501876268322368 1.854443318793108 7 0.37819756440096075 1.487196181199987 8 0.2740197272357171 1.2314685090624715 9 0.20754797673560232 1.0493310100475721 >10 1.1647607813300256 11.972800476840927 >50 0.0764053725739866 2.9812376834755367 >100 0.03866295611575258 3.752963416386256 >500 0.0018410931483691156 0.7031204195957773 >1k 0.0012273954322460769 1.1102717878441777 >5k 6.136977161230384E-4 2.20485738834263 >10k+ 3.068488580615192E-4 1.7370297789327402 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10077 1.7280495521681645 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7677 1.3164866936583308 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5114 0.8769718576747042 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1981 0.339710842794992 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1634 0.28020571283544515 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1438 0.2465947460571421 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1388 0.2380205198381872 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 817 0.14010285641772258 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 720 0.12346885755295013 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 701 0.12021065158974728 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 698 0.11969619801660998 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 588 0.10083290033490928 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.57422621895487E-5 0.0 18-19 0.0 0.0 0.0 3.429690487581948E-4 0.0 20-21 0.0 0.0 0.0 6.001958353268409E-4 0.0 22-23 0.0 0.0 0.0 0.0012003916706536818 0.0 24-25 0.0 0.0 0.0 0.0018005875059805229 0.0 26-27 0.0 0.0 0.0 0.002486525603496912 0.0 28-29 0.0 0.0 0.0 0.00531602025575202 0.0 30-31 0.0 0.0 0.0 0.010803525035883138 0.0 32-33 0.0 0.0 0.0 0.015605091718497863 0.0 34-35 0.0 0.0 0.0 0.01980646256578575 0.0 36-37 0.0 0.0 0.0 0.026408616754380998 0.0 38-39 0.0 0.0 0.0 0.03764085310121188 0.0 40-41 0.0 0.0 0.0 0.055389501374448466 0.0 42-43 0.0 0.0 0.0 0.09165847828062756 0.0 44-45 0.0 0.0 0.0 0.12415479565046653 0.0 46-47 0.0 0.0 0.0 0.2026947078160931 0.0 48-49 0.0 0.0 0.0 0.2751469193662618 0.0 50-51 0.0 0.0 0.0 0.32513465822276866 0.0 52-53 0.0 0.0 0.0 0.3957005400047673 0.0 54-55 0.0 0.0 0.0 0.4664379063111449 0.0 56-57 0.0 0.0 0.0 0.6354359050867455 0.0 58-59 0.0 0.0 0.0 0.8119792229350262 0.0 60-61 0.0 0.0 0.0 1.0150168997998776 0.0 62-63 0.0 0.0 0.0 1.2031354230437474 0.0 64-65 0.0 0.0 0.0 1.40591587312203 0.0 66-67 0.0 0.0 0.0 1.7149309860531636 0.0 68-69 0.0 0.0 0.0 2.1110602373688785 0.0 70-71 0.0 0.0 0.0 2.3823487549366105 0.0 72-73 0.0 0.0 0.0 2.629715181353459 0.0 74-75 0.0 0.0 0.0 2.936758222254233 0.0 76-77 0.0 0.0 0.0 3.2732966013482114 0.0 78-79 0.0 0.0 0.0 3.597488094686895 0.0 80-81 0.0 0.0 0.0 4.136635439334777 0.0 82-83 0.0 0.0 0.0 4.782446158146458 0.0 84-85 0.0 0.0 0.0 5.527117705262689 0.0 86-87 0.0 0.0 0.0 6.167440919294238 0.0 88-89 0.0 0.0 0.0 6.922658764659783 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4640 0.0 44.84646 1 GTATCAA 7650 0.0 37.261627 1 CCGTTGC 90 2.4093762E-5 31.66967 9 CATATAG 110 2.7338665E-6 30.22236 3 ATCAACG 9465 0.0 30.00569 3 TATCAAC 9510 0.0 29.913647 2 TCAACGC 9555 0.0 29.673357 4 CAACGCA 9680 0.0 29.24864 5 AACGCAG 9830 0.0 28.802324 6 ACGCAGA 10915 0.0 25.8522 7 CGCAGAG 10915 0.0 25.765156 8 GAGTACT 6715 0.0 25.638054 12-13 GCAGAGT 11110 0.0 24.928108 9 TATTCCG 115 1.2798703E-4 24.784958 5 TTCAACG 120 1.7112927E-4 23.74614 2 GTACTTT 7230 0.0 23.713297 14-15 AGTACTT 6855 0.0 23.17457 12-13 GTACATG 4335 0.0 23.014536 1 GGATAAG 250 3.8016879E-10 22.80412 1 AGAGTAC 9515 0.0 22.291918 10-11 >>END_MODULE