FastQCFastQC Report
Thu 26 May 2016
SRR936832_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936832_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618351
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67971.0992138769080992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47190.7631587884551008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32300.5223570431680389No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22590.36532648932402473No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10150.16414625350326917No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9730.15735399473761666No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9190.14862109061034914No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7540.12193721688814282No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69050.036.3710021
GGTATCA42800.032.228441
ATCAACG84300.029.6245633
TCAACGC84650.029.5582714
TATCAAC85350.029.3158472
CAACGCA86400.028.9595785
AACGCAG87350.028.5808936
ACGCAGA96050.025.8909857
CGCAGAG96450.025.687098
AGTACTT44100.024.96271312-13
GAGTACT43700.024.53830312-13
GCAGAGT98050.024.4899589
GTACATG51200.023.6894651
TACATGG52550.022.8111952
GTACTTT47100.022.71371814-15
TTGAGCG1050.00218188122.6425299
AGAGTAC79300.022.27562310-11
ACATGGG52150.021.8915823
TACTTTT50450.021.865214-15
TACCTGG2858.185452E-1121.697952
Produced by FastQC (version 0.11.5)