FastQCFastQC Report
Thu 26 May 2016
SRR936832_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936832_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618351
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105101.699681895881142No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80871.307833253281712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56360.9114564381718473No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21780.3522271331331234No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17840.2885092770934307No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14430.23336260473420437No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13330.21557335558606683No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8050.1301849596750066No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.12549506671777033No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.11757076482450907No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6630.10722065622922902No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.10625033354842153No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6400.10350108595280026No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47000.042.9669761
GTATCAA75900.037.1867641
CGGGTCG953.480039E-530.0103726
TATCAAC95900.029.374772
ATCAACG97050.028.9241123
TCAACGC98200.028.5853884
CAACGCA100100.028.0428095
AACGCAG101600.027.6399676
TTTACGC700.00839102927.141263
CGGTTGC458.9552306E-426.38520428-29
ACGCAGA112050.025.1046147
GAGTACT67800.025.08877812-13
CGCAGAG111700.024.9726038
AGTACTT68000.024.56080412-13
GCAGAGT114050.024.1663789
GTACATG45350.023.9939611
GTACTTT72400.023.32406614-15
GTGCATA2653.092282E-1123.3099421
GGGCCGT1252.2489941E-422.8078846
TAGGGTC1252.2551623E-422.7986625
Produced by FastQC (version 0.11.5)