##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936832_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 618351 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11708398627964 33.0 31.0 34.0 31.0 34.0 2 32.21058266259778 33.0 31.0 34.0 30.0 34.0 3 32.301226973029884 34.0 31.0 34.0 30.0 34.0 4 36.02628118980967 37.0 35.0 37.0 35.0 37.0 5 35.892184212526544 37.0 35.0 37.0 35.0 37.0 6 35.99698391366716 37.0 35.0 37.0 35.0 37.0 7 35.95138845089601 37.0 35.0 37.0 35.0 37.0 8 35.91198849844182 37.0 35.0 37.0 35.0 37.0 9 37.58614282179539 39.0 37.0 39.0 35.0 39.0 10-11 37.50494055964978 39.0 37.0 39.0 35.0 39.0 12-13 37.38772153679706 39.0 37.0 39.0 34.0 39.0 14-15 38.4801383033261 40.0 38.0 41.0 34.0 41.0 16-17 38.49796959979041 40.0 38.0 41.0 34.0 41.0 18-19 38.71159745840146 40.0 38.0 41.0 34.5 41.0 20-21 38.76376685733507 40.0 38.0 41.0 35.0 41.0 22-23 38.663059492100764 40.0 38.0 41.0 34.0 41.0 24-25 38.554086594830444 40.0 38.0 41.0 34.0 41.0 26-27 38.326419784232584 40.0 38.0 41.0 34.0 41.0 28-29 38.13952188967107 40.0 37.5 41.0 34.0 41.0 30-31 37.925997532145985 40.0 37.0 41.0 33.5 41.0 32-33 37.620492244695974 40.0 36.0 41.0 33.0 41.0 34-35 37.40943816699577 40.0 35.0 41.0 33.0 41.0 36-37 37.17509553635395 39.5 35.0 41.0 33.0 41.0 38-39 36.947594489214055 39.0 35.0 41.0 32.0 41.0 40-41 36.51383841863279 39.0 35.0 41.0 30.5 41.0 42-43 36.18458124916107 39.0 35.0 41.0 30.0 41.0 44-45 35.71847704620838 39.0 35.0 40.5 29.5 41.0 46-47 34.98424195966369 39.0 35.0 40.0 25.0 41.0 48-49 34.297630310293016 38.0 34.5 40.0 21.0 41.0 50-51 33.915793780555056 38.0 34.0 40.0 16.5 41.0 52-53 33.63166551036547 38.0 34.0 40.0 11.5 41.0 54-55 33.26023730858364 38.0 33.0 40.0 8.0 41.0 56-57 33.04183869679195 38.0 33.0 40.0 7.0 41.0 58-59 32.73249335733264 37.0 33.0 40.0 2.0 41.0 60-61 32.347179029386226 37.0 32.0 40.0 2.0 41.0 62-63 31.992466252985764 36.0 31.5 39.0 2.0 40.5 64-65 31.680034478799257 36.0 31.0 39.0 2.0 40.0 66-67 31.36103442866592 35.5 31.0 39.0 2.0 40.0 68-69 30.91694118712511 35.0 31.0 38.5 2.0 40.0 70-71 30.47623032872915 35.0 30.0 38.0 2.0 40.0 72-73 30.066568987516796 35.0 29.5 37.0 2.0 39.0 74-75 29.60694977448084 34.0 29.0 36.5 2.0 39.0 76-77 28.197146119275295 32.5 27.0 35.0 2.0 37.5 78-79 28.811978956935462 34.0 29.0 36.0 2.0 38.0 80-81 28.66112450695479 34.0 29.0 35.0 2.0 37.0 82-83 28.30933240182356 34.0 28.5 35.0 2.0 37.0 84-85 27.875533475323884 34.0 27.0 35.0 2.0 36.0 86-87 27.483371903659894 34.0 26.5 35.0 2.0 36.0 88-89 27.102574427792632 33.0 26.0 35.0 2.0 35.5 90-91 26.633927170813987 33.0 25.0 35.0 2.0 35.0 92-93 26.160421831613437 33.0 24.5 35.0 2.0 35.0 94-95 25.632729630905423 32.0 23.0 34.0 2.0 35.0 96-97 24.905194622471704 32.0 18.5 34.0 2.0 35.0 98-99 24.09718347669851 31.5 2.0 34.0 2.0 35.0 100-101 21.230115258162435 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2652453715012868 1101 2 0.4956172508604979 1101 3 0.43186135268943815 1101 4 0.2507110881388428 1101 5 0.26311640030650096 1101 6 0.304685867083883 1101 7 0.24888387542166157 1101 8 0.3601371730963763 1101 9 0.4349160089493509 1101 10-11 0.44659191214635996 1101 12-13 0.3674945376977874 1101 14-15 0.5748700053731497 1101 16-17 0.5451665557551948 1101 18-19 0.2675418511899821 1101 20-21 0.18061286779726515 1101 22-23 0.3930218652941804 1101 24-25 0.2842837057760761 1101 26-27 0.5200662385149286 1101 28-29 0.37202797472498617 1101 30-31 0.521702046719831 1101 32-33 0.5556499043222161 1101 34-35 0.495447746201755 1101 36-37 0.48781439546732486 1101 38-39 0.5414288408628352 1101 40-41 0.5531293185214068 1101 42-43 0.43403099640057974 1101 44-45 0.2694901250282271 1101 46-47 0.18414645730365464 1101 48-49 0.4047180197254221 1101 50-51 0.46818881809714696 1101 52-53 0.5137690671510953 1101 54-55 0.07391022185898777 1101 56-57 0.17666435544165893 1101 58-59 0.2804810909710227 1101 60-61 0.36471986964913583 1101 62-63 0.2517100975834907 1101 64-65 0.2738330867897183 1101 66-67 0.1412690528848053 1101 68-69 -0.04289585153956921 1101 70-71 0.058356446643383464 1101 72-73 0.1506910710814502 1101 74-75 0.14723465058641594 1101 76-77 0.23398881783294456 1101 78-79 0.3682377554533538 1101 80-81 0.37525578021614336 1101 82-83 0.6846740476579569 1101 84-85 0.5799589353547958 1101 86-87 0.4082802724054879 1101 88-89 0.5259795173526136 1101 90-91 0.8872255469604049 1101 92-93 0.615749241566693 1101 94-95 0.3550258272236704 1101 96-97 0.19411400664696643 1101 98-99 0.27657291188623034 1101 100-101 0.8346964339429555 1102 1 -0.13036507532061492 1102 2 -0.15759231490413583 1102 3 -0.17834422733992739 1102 4 -0.04723835595597592 1102 5 -0.18273528838659558 1102 6 0.08774649221498976 1102 7 0.1699925391514725 1102 8 0.15683320833866077 1102 9 0.18821997370706356 1102 10-11 0.1051559985738848 1102 12-13 0.23639531386728407 1102 14-15 0.2338379097155041 1102 16-17 0.23342436989405968 1102 18-19 0.21448175049772544 1102 20-21 0.1194003651750748 1102 22-23 0.0031068243879488477 1102 24-25 -0.05969835841523974 1102 26-27 0.1468886635201727 1102 28-29 -0.0013043097306493223 1102 30-31 0.19677022377039322 1102 32-33 0.043401634970422265 1102 34-35 0.20781663648914872 1102 36-37 0.29198368432824395 1102 38-39 0.42701463905876835 1102 40-41 0.2887151167173414 1102 42-43 0.059298460214307624 1102 44-45 0.05746998657636482 1102 46-47 0.2863753224179817 1102 48-49 0.6115619467235334 1102 50-51 0.34797170041307623 1102 52-53 0.45570844201998995 1102 54-55 0.6127344299563191 1102 56-57 0.49322613433822937 1102 58-59 0.45629397118919 1102 60-61 0.4648824456927656 1102 62-63 0.28642270509345025 1102 64-65 0.35066337837771755 1102 66-67 0.5134528162579954 1102 68-69 0.5680046099666178 1102 70-71 0.3764862977027512 1102 72-73 0.31250143399313046 1102 74-75 0.34964182490988804 1102 76-77 0.3842457928696561 1102 78-79 0.1820016530828923 1102 80-81 0.4551540390791615 1102 82-83 0.7051586291557541 1102 84-85 0.5747932126780597 1102 86-87 0.7017667418831479 1102 88-89 0.6457298844598078 1102 90-91 0.43842126753938615 1102 92-93 0.5088345153237732 1102 94-95 0.5597929763740765 1102 96-97 0.7760680658036705 1102 98-99 0.4390781562747357 1102 100-101 0.5191573108047152 1103 1 -0.014493044126005827 1103 2 0.08799435648898424 1103 3 0.011440718896629676 1103 4 -0.0630808467984707 1103 5 -0.06352519740231344 1103 6 0.02386252671811917 1103 7 -0.08846486366076789 1103 8 0.04976124804089466 1103 9 -0.020837098253231545 1103 10-11 0.032250409539265945 1103 12-13 0.09803421260295409 1103 14-15 0.06872801960561503 1103 16-17 0.09101624038915901 1103 18-19 -0.0353188521254566 1103 20-21 -0.025500970048845772 1103 22-23 0.1680926450189304 1103 24-25 0.049894870936050495 1103 26-27 -0.019888779466946005 1103 28-29 -0.11419867343145995 1103 30-31 0.06585329124055761 1103 32-33 0.10070844106755317 1103 34-35 0.057897872457481014 1103 36-37 0.26759516961715235 1103 38-39 0.15164981571007274 1103 40-41 0.31006245217758277 1103 42-43 0.13699254032773922 1103 44-45 -0.020392473544156076 1103 46-47 -0.3486595351975268 1103 48-49 0.027389667389961403 1103 50-51 0.2511000237026906 1103 52-53 0.314133350444898 1103 54-55 0.49223342558435235 1103 56-57 0.039403612773782015 1103 58-59 0.05966747940140493 1103 60-61 -0.024604142987371347 1103 62-63 0.12049159319460045 1103 64-65 -0.07752402561613891 1103 66-67 -0.06226501429854281 1103 68-69 -0.1306941375224504 1103 70-71 0.09600360834909694 1103 72-73 0.13162290392105191 1103 74-75 -0.2035308089079102 1103 76-77 0.03960145870919263 1103 78-79 -0.08760280160220546 1103 80-81 -0.1738486786332345 1103 82-83 0.06409163163714382 1103 84-85 -0.09685100332099594 1103 86-87 -0.262261971339111 1103 88-89 -0.03433628592894422 1103 90-91 -0.18038412062083964 1103 92-93 0.1266517787055541 1103 94-95 0.45925238793026324 1103 96-97 0.34704466984479154 1103 98-99 0.30046448499977174 1103 100-101 0.6888164151412361 1104 1 -0.0385948889852088 1104 2 0.014250335199385233 1104 3 -0.029793502702247565 1104 4 -0.03911206196011818 1104 5 -0.2200541289663107 1104 6 -0.1425847707249659 1104 7 -0.044137895631138235 1104 8 0.0966882808024323 1104 9 -0.04004089585815507 1104 10-11 -0.04298064738884477 1104 12-13 -0.03555085590497953 1104 14-15 -0.10161757682693917 1104 16-17 0.07131256865182678 1104 18-19 0.03226125360331622 1104 20-21 0.018779247162648005 1104 22-23 -0.16506087747540477 1104 24-25 0.03458217988703893 1104 26-27 -0.004534731924962898 1104 28-29 -0.030031017805178806 1104 30-31 0.0712008655923384 1104 32-33 -0.047203955091688954 1104 34-35 0.04630007334215236 1104 36-37 -0.05936207136534222 1104 38-39 -0.048373559698994484 1104 40-41 0.14702257013348685 1104 42-43 0.17021978526605608 1104 44-45 -0.2054699720157629 1104 46-47 -0.23432861132943117 1104 48-49 0.06000915169247634 1104 50-51 0.01106693850830709 1104 52-53 -0.12338058489512349 1104 54-55 0.07950461629172878 1104 56-57 0.08473752150179337 1104 58-59 0.036935793135363326 1104 60-61 0.05354704196605553 1104 62-63 -0.21745928248418878 1104 64-65 -0.13360163197424768 1104 66-67 -0.4122200615887941 1104 68-69 -0.23083079376008797 1104 70-71 0.043308243872527186 1104 72-73 0.06679750024572328 1104 74-75 -0.1603995829576057 1104 76-77 0.10489796678270125 1104 78-79 0.20816624935618577 1104 80-81 0.1536012440481045 1104 82-83 0.20733254219923225 1104 84-85 0.16832323338199373 1104 86-87 -0.05646307178144738 1104 88-89 -0.3499222894532359 1104 90-91 0.06606101909218154 1104 92-93 0.1374835836467554 1104 94-95 0.02806419790616843 1104 96-97 0.20386309685957116 1104 98-99 -0.1377075580109839 1104 100-101 0.9843229422746944 1105 1 -0.002873380968452466 1105 2 0.024467485760318652 1105 3 -0.11893115978732283 1105 4 -0.07777545451633472 1105 5 -0.07877626291594453 1105 6 0.013936968405467098 1105 7 0.17295488688126426 1105 8 0.0918830422257102 1105 9 -0.0501521193161949 1105 10-11 -0.04905219633198499 1105 12-13 0.1722203415527801 1105 14-15 0.2096684744995585 1105 16-17 -0.16851361460205538 1105 18-19 -0.027004960465731642 1105 20-21 0.15422871511970015 1105 22-23 0.021960644985959732 1105 24-25 -0.07576978698666892 1105 26-27 0.030678807484733284 1105 28-29 -0.06849700076054432 1105 30-31 0.1259507331834584 1105 32-33 0.07965578779434423 1105 34-35 0.17402649252458957 1105 36-37 0.22038899994285543 1105 38-39 0.005738337840611507 1105 40-41 0.037787652708480834 1105 42-43 -0.25988758629732445 1105 44-45 -0.21794529581565314 1105 46-47 -0.3878217982907728 1105 48-49 -0.4140194486253037 1105 50-51 -0.21274258530121415 1105 52-53 -0.3744687451339459 1105 54-55 -0.05481817247999743 1105 56-57 -0.31818028869609805 1105 58-59 -0.4004612890544408 1105 60-61 -0.3576812309406776 1105 62-63 -0.31936356290211876 1105 64-65 -0.40653484975074505 1105 66-67 -0.2825604727785489 1105 68-69 -0.14720856832795448 1105 70-71 -0.27547937228617414 1105 72-73 -0.3611142913667784 1105 74-75 -0.4036804342263238 1105 76-77 -0.19712187047475282 1105 78-79 0.013217046216890793 1105 80-81 -0.1674926940326884 1105 82-83 -0.20414369642564267 1105 84-85 -0.38021786151906056 1105 86-87 -0.26924654715339713 1105 88-89 -0.003661034698556165 1105 90-91 -0.3511136161815429 1105 92-93 -0.27733658102174275 1105 94-95 0.06269995311826193 1105 96-97 -0.08863293087406987 1105 98-99 -0.16400877617266474 1105 100-101 0.1972580859985129 1106 1 -0.0722664837713225 1106 2 -0.08193839136288972 1106 3 0.08124665126503317 1106 4 0.06592395122041239 1106 5 -0.11239658952677445 1106 6 0.07832328631290864 1106 7 0.14214210320244547 1106 8 0.13097807755395507 1106 9 0.13238496364670027 1106 10-11 0.12863017697763723 1106 12-13 0.011277942975262079 1106 14-15 0.1972684932165052 1106 16-17 0.14043308887461592 1106 18-19 0.08030167001482624 1106 20-21 0.06852846712007477 1106 22-23 0.19168831332154923 1106 24-25 0.08871713454250596 1106 26-27 0.30705801295009394 1106 28-29 0.2892892516375625 1106 30-31 0.23523434181197445 1106 32-33 0.18075072953782723 1106 34-35 0.09181227699887273 1106 36-37 0.21683277889565034 1106 38-39 0.1273751353699737 1106 40-41 0.3058261160466529 1106 42-43 0.38017371172335856 1106 44-45 0.10515610327657043 1106 46-47 0.0990178442154317 1106 48-49 -0.152824371183911 1106 50-51 -0.1141510360054383 1106 52-53 -0.15498907642400894 1106 54-55 -0.0721079577029613 1106 56-57 -0.24614341629892067 1106 58-59 -0.419997108623015 1106 60-61 -0.3360397541730933 1106 62-63 -0.4707369193196911 1106 64-65 -0.38240167863100183 1106 66-67 -0.42306629843013255 1106 68-69 -0.5542204068006491 1106 70-71 -0.32745401081502123 1106 72-73 -0.3623308933039837 1106 74-75 -0.8445231650297522 1106 76-77 -0.5329741937165267 1106 78-79 -0.7418833234429307 1106 80-81 -0.5601041768832786 1106 82-83 -0.40077698452633825 1106 84-85 -0.27589424540376584 1106 86-87 -0.46055720177882264 1106 88-89 -0.3103734019251725 1106 90-91 -0.44598517109240277 1106 92-93 -0.23681437803638872 1106 94-95 -0.06067986064805453 1106 96-97 0.05790911208133309 1106 98-99 0.0653440851815077 1106 100-101 0.4881546280414675 1107 1 0.24740095136341012 1107 2 0.12239466849970171 1107 3 0.1423467369729039 1107 4 0.03916201284757648 1107 5 0.07248490903177895 1107 6 0.1050531415261986 1107 7 0.07760453611426499 1107 8 0.04988560013821086 1107 9 0.062365759584054103 1107 10-11 0.0987561721285033 1107 12-13 0.020119145085423895 1107 14-15 -0.059710739812643965 1107 16-17 0.16678482113606918 1107 18-19 0.056431799309962116 1107 20-21 -0.1109011859693041 1107 22-23 -0.24924429829346906 1107 24-25 0.13420417982100474 1107 26-27 0.311636738148664 1107 28-29 0.17354925356255535 1107 30-31 0.02833004587690624 1107 32-33 0.27667937659099096 1107 34-35 0.2546045202075575 1107 36-37 0.15477249005554938 1107 38-39 0.19191728576665668 1107 40-41 0.29843553107898657 1107 42-43 0.4435402434879734 1107 44-45 0.2456574255610775 1107 46-47 0.3789938276788547 1107 48-49 0.4406865806317697 1107 50-51 0.45989002127217304 1107 52-53 0.35776397949693717 1107 54-55 0.3233971916441476 1107 56-57 0.28592599332510815 1107 58-59 0.24250180284121114 1107 60-61 0.5526820985835315 1107 62-63 0.7971803794616079 1107 64-65 0.6841594191630556 1107 66-67 0.4900066724246095 1107 68-69 0.2929056295999857 1107 70-71 0.06265887607350962 1107 72-73 0.3232862262699534 1107 74-75 0.4196060292704473 1107 76-77 0.09075680274057518 1107 78-79 0.40582066816044815 1107 80-81 0.7133978833138954 1107 82-83 0.788188241912021 1107 84-85 0.59563332548856 1107 86-87 1.1208767321207027 1107 88-89 1.2912500059480365 1107 90-91 1.1397066564101976 1107 92-93 1.1154770470517263 1107 94-95 1.188372540318106 1107 96-97 1.2938601256800126 1107 98-99 1.3875392149578865 1107 100-101 1.6420529610921655 1108 1 0.0025714326158947642 1108 2 0.05312197081015313 1108 3 6.24026628322838E-4 1108 4 -0.0841431178607408 1108 5 -0.24225909791040579 1108 6 -0.26185668238818494 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96-97 -0.45967573584922405 2316 98-99 -0.8046693840637182 2316 100-101 -1.957810418435745 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 6.0 5 15.0 6 42.0 7 122.0 8 261.0 9 558.0 10 928.0 11 1719.0 12 2599.0 13 3597.0 14 4743.0 15 6653.0 16 9485.0 17 14094.0 18 12397.0 19 5938.0 20 4513.0 21 4381.0 22 4654.0 23 4605.0 24 5369.0 25 5991.0 26 6960.0 27 8375.0 28 10243.0 29 12408.0 30 15046.0 31 19077.0 32 23438.0 33 30450.0 34 41587.0 35 59875.0 36 85193.0 37 124358.0 38 83909.0 39 4761.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.620632984694616 19.754324554280554 16.31312134632991 19.31192111469491 2 15.394642571229413 22.213452926402066 40.965909182800466 21.425995319568056 3 18.20650284226443 28.205451649160256 32.77797993029781 20.8100655782775 4 11.729907447388294 18.823451405431545 38.9678354203357 30.47880572684446 5 11.543686462743677 38.94374921159625 34.05402870274833 15.45853562291174 6 27.314421744284395 39.105459520563564 17.978138630001407 15.601980105150634 7 24.55906848871998 37.15436241610738 21.45742702353036 16.829142071642273 8 26.279896046096795 33.42324990175483 20.164760791201115 20.13209326094726 9 27.533714670146892 16.168486830295414 21.33351446023375 34.96428403932394 10-11 23.698271693585035 28.294366791676573 26.877129656133814 21.130231858604578 12-13 26.4592024959101 26.716201015216935 28.249620122547377 18.574976366325593 14-15 22.96720207207797 26.120845877475368 29.748357030743968 21.163595019702694 16-17 19.01444325310382 30.000840946323365 29.682655967241907 21.302059833330908 18-19 18.82504863758169 26.237124989285988 36.00119026633827 18.93663610679406 20-21 20.510463086276896 24.89251472287518 36.29262517981597 18.30439701103195 22-23 19.772745834199227 25.31477499783695 36.37134029338299 18.54113887458083 24-25 19.503592264804702 25.301673425326577 36.616438300779905 18.578296009088813 26-27 19.328032111367712 25.468425546779482 36.605843311533306 18.597699030319497 28-29 18.86877890254621 25.889969992698305 36.536699714320136 18.704551390435345 30-31 19.085923690589972 25.680964371368365 36.4851839812663 18.74792795677536 32-33 19.128048341671402 25.64815795623342 36.26951468306303 18.954279019032146 34-35 19.19185058324479 25.727701580493928 36.16683728173804 18.913610554523242 36-37 19.428355180859675 25.449005682162763 36.18519629620345 18.937442840774107 38-39 19.271456286811116 25.881465271493486 36.02267277722112 18.824405664474277 40-41 19.123027896306827 25.931622148530714 35.76903452530835 19.176315429854117 42-43 19.214734026467166 26.028663331991055 35.31594515089326 19.440657490648515 44-45 19.463601718453972 26.287445631211625 34.729799554133486 19.519153096200917 46-47 19.82126583957105 26.690854752619273 33.96046080810417 19.527418599705506 48-49 20.1647934017951 27.127355057815155 32.77278240478693 19.935069135602816 50-51 19.954830054752186 27.38692756231892 32.520419871864846 20.137822511064048 52-53 20.03297495908453 27.813140820379463 31.86627918259621 20.2876050379398 54-55 19.816091507897617 27.78834351363546 31.701412304661915 20.694152673805007 56-57 20.095512327575264 28.06155742019683 31.3300164389233 20.51291381330461 58-59 20.371165497829303 27.500871271020678 31.668983317686845 20.45897991346318 60-61 20.159411582528282 27.305601399885017 32.308136427333665 20.226850590253044 62-63 20.01227459183748 27.39667874449847 32.4965371581328 20.094509505531246 64-65 19.951564647853157 27.60152017465837 32.163257055065905 20.283658122422576 66-67 20.114886298304928 27.68130696101968 31.714213864143392 20.489592876531997 68-69 20.23645203885505 27.434484866568177 31.89717142166364 20.43189167291313 70-71 20.19524667419212 27.77781371594544 31.639324908708073 20.38761470115437 72-73 20.25288410300697 27.694666339988988 31.42154115855754 20.6309083984465 74-75 19.986414856324977 27.94569177088008 31.255776718460027 20.812116654334915 76-77 19.79977375291198 28.001998866338752 31.33473652887804 20.863490851871227 78-79 19.637308087591382 28.1534603868207 31.152012174364458 21.05721935122346 80-81 19.645999236656035 28.181462385902716 30.881468208017697 21.291070169423545 82-83 19.623918492763 27.925851055227625 31.07794938141829 21.37228107059109 84-85 19.41306517059633 27.82320472368359 30.940386297953687 21.823343807766392 86-87 19.47809155391965 27.705524091689764 30.82206402240817 21.99432033198242 88-89 19.207393498909152 27.554271445367906 30.724438686973077 22.51389636874987 90-91 19.15893556168132 27.541763330748438 31.0616162261426 22.23768488142764 92-93 18.862021731993643 27.60762091433506 31.128598482091885 22.40175887157941 94-95 18.732887955222843 27.72769834608499 30.789632425596462 22.7497812730957 96-97 19.038809083888914 27.627426325811943 30.561860442550508 22.771904147748636 98-99 18.905203099250738 27.548868508255126 30.3590402090749 23.186888183419235 100-101 18.51743436187753 27.12735117861154 30.378105019378697 23.977109440132228 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1596.0 1 1099.0 2 685.0 3 750.5 4 581.5 5 511.0 6 923.5 7 2151.5 8 3625.5 9 4108.0 10 3362.5 11 2477.5 12 2252.5 13 2306.0 14 2407.5 15 2499.5 16 2604.5 17 2723.0 18 2822.5 19 3014.5 20 3169.5 21 3314.5 22 3599.5 23 4113.5 24 4661.0 25 5304.0 26 6225.5 27 7017.5 28 7632.0 29 8448.5 30 9795.5 31 11404.5 32 12906.0 33 14035.0 34 15546.5 35 16844.5 36 17994.5 37 19527.5 38 19750.0 39 19128.5 40 19239.0 41 19586.5 42 19503.5 43 19757.5 44 20279.0 45 20451.0 46 20059.0 47 20208.0 48 22157.0 49 21435.0 50 18203.0 51 16750.5 52 16028.0 53 15657.0 54 14796.0 55 13078.5 56 11429.5 57 10122.0 58 9316.5 59 8291.0 60 7134.0 61 5951.0 62 4658.5 63 3554.5 64 2477.5 65 1755.0 66 1321.5 67 1023.5 68 682.0 69 364.0 70 247.5 71 194.0 72 146.5 73 113.0 74 83.5 75 67.5 76 55.5 77 28.5 78 9.5 79 4.5 80 2.5 81 3.0 82 2.5 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02215570121177131 2 0.0051750542976400134 3 9.703226808075025E-4 4 0.0 5 0.0021023658084162555 6 0.0 7 1.6172044680125042E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0096223665846744 14-15 0.0055793554146431395 16-17 0.0 18-19 3.2344089360250084E-4 20-21 0.0016980646914131292 22-23 0.0020215055850156304 24-25 0.0013746237978106287 26-27 4.8516134040375126E-4 28-29 2.4258067020187563E-4 30-31 0.0 32-33 4.04301117003126E-4 34-35 0.0 36-37 0.0020215055850156304 38-39 0.012371614180295656 40-41 0.0012129033510093782 42-43 0.0 44-45 7.277420106056269E-4 46-47 4.04301117003126E-4 48-49 1.6172044680125042E-4 50-51 0.003153548712624383 52-53 4.8516134040375126E-4 54-55 0.0 56-57 2.4258067020187563E-4 58-59 2.4258067020187563E-4 60-61 0.0023449464786181313 62-63 8.086022340062521E-5 64-65 1.6172044680125042E-4 66-67 8.086022340062521E-5 68-69 2.4258067020187563E-4 70-71 8.08602234006252E-4 72-73 7.277420106056269E-4 74-75 0.004609032733835637 76-77 8.086022340062521E-5 78-79 4.04301117003126E-4 80-81 0.003072688489223758 82-83 1.6172044680125042E-4 84-85 0.003234408936025008 86-87 8.08602234006252E-4 88-89 0.00388129072323001 90-91 0.0025066669254193816 92-93 0.0 94-95 0.0 96-97 5.660215638043766E-4 98-99 0.0012937635744100034 100-101 0.0028301078190218825 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 618351.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.70566655603424 #Duplication Level Percentage of deduplicated Percentage of total 1 79.38469324140412 44.22177251358724 2 11.612731181203628 12.93789861969982 3 3.7433902818407665 6.255841524879624 4 1.6338739395281612 3.640641474797992 5 0.9065650303706405 2.5250404646593982 6 0.5573771371498742 1.862943896881672 7 0.3894744542592339 1.5187153856740805 8 0.27202271358739083 1.2122565263013438 9 0.2024164252464049 1.0148167701216595 >10 1.175325797003856 12.17221048756553 >50 0.07790534564123665 3.00308750573596 >100 0.03983129520522139 3.7108281011015376 >500 0.0023430173650123513 0.8710534724896718 >1k 0.0011715086825061756 1.099299175432742 >5k 5.857543412530878E-4 2.2388961983472124 >10k+ 2.928771706265439E-4 1.7146978827245647 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10510 1.699681895881142 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8087 1.307833253281712 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5636 0.9114564381718473 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2178 0.3522271331331234 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1784 0.2885092770934307 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1443 0.23336260473420437 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1333 0.21557335558606683 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 805 0.1301849596750066 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 776 0.12549506671777033 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 727 0.11757076482450907 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 663 0.10722065622922902 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 657 0.10625033354842153 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 640 0.10350108595280026 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.6172044680125042E-4 0.0 12-13 0.0 0.0 0.0 1.6172044680125042E-4 0.0 14-15 0.0 0.0 0.0 1.6172044680125042E-4 0.0 16-17 0.0 0.0 0.0 1.6172044680125042E-4 0.0 18-19 0.0 0.0 0.0 1.6172044680125042E-4 0.0 20-21 0.0 0.0 0.0 3.2344089360250084E-4 0.0 22-23 0.0 0.0 0.0 8.086022340062522E-4 0.0 24-25 0.0 0.0 0.0 0.0017789249148137546 0.0 26-27 0.0 0.0 0.0 0.0025066669254193816 0.0 28-29 0.0 0.0 0.0 0.004689892957236262 0.0 30-31 0.0 0.0 0.0 0.010835269935683779 0.0 32-33 0.0 0.0 0.0 0.016657206020528795 0.0 34-35 0.0 0.0 0.0 0.021670539871367557 0.0 36-37 0.0 0.0 0.0 0.02749247595621257 0.0 38-39 0.0 0.0 0.0 0.0388129072323001 0.0 40-41 0.0 0.0 0.0 0.0596748448696614 0.0 42-43 0.0 0.0 0.0 0.09266581601711649 0.0 44-45 0.0 0.0 0.0 0.11902624884572031 0.0 46-47 0.0 0.0 0.0 0.196975504203923 0.0 48-49 0.0 0.0 0.0 0.2714477699558988 0.0 50-51 0.0 0.0 0.0 0.31996390399627395 0.0 52-53 0.0 0.0 0.0 0.3907974596952216 0.0 54-55 0.0 0.0 0.0 0.46114585405376557 0.0 56-57 0.0 0.0 0.0 0.6214916770572054 0.0 58-59 0.0 0.0 0.0 0.7781179297842165 0.0 60-61 0.0 0.0 0.0 0.9605385937760269 0.0 62-63 0.0 0.0 0.0 1.1402100101722161 0.0 64-65 0.0 0.0 0.0 1.3342745463337167 0.0 66-67 0.0 0.0 0.0 1.6301421037566044 0.0 68-69 0.0 0.0 0.0 2.0114789173139527 0.0 70-71 0.0 0.0 0.0 2.2511486194734056 0.0 72-73 0.0 0.0 0.0 2.475535739410141 0.0 74-75 0.0 0.0 0.0 2.7519968432168787 0.0 76-77 0.0 0.0 0.0 3.080127629776616 0.0 78-79 0.0 0.0 0.0 3.380119058592935 0.0 80-81 0.0 0.0 0.0 3.8714257759751343 0.0 82-83 0.0 0.0 0.0 4.466233579310133 0.0 84-85 0.0 0.0 0.0 5.176024620320821 0.0 86-87 0.0 0.0 0.0 5.7715601656664255 0.0 88-89 0.0 0.0 0.0 6.5102183064311365 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4700 0.0 42.966976 1 GTATCAA 7590 0.0 37.186764 1 CGGGTCG 95 3.480039E-5 30.010372 6 TATCAAC 9590 0.0 29.37477 2 ATCAACG 9705 0.0 28.924112 3 TCAACGC 9820 0.0 28.585388 4 CAACGCA 10010 0.0 28.042809 5 AACGCAG 10160 0.0 27.639967 6 TTTACGC 70 0.008391029 27.14126 3 CGGTTGC 45 8.9552306E-4 26.385204 28-29 ACGCAGA 11205 0.0 25.104614 7 GAGTACT 6780 0.0 25.088778 12-13 CGCAGAG 11170 0.0 24.972603 8 AGTACTT 6800 0.0 24.560804 12-13 GCAGAGT 11405 0.0 24.166378 9 GTACATG 4535 0.0 23.993961 1 GTACTTT 7240 0.0 23.324066 14-15 GTGCATA 265 3.092282E-11 23.309942 1 GGGCCGT 125 2.2489941E-4 22.807884 6 TAGGGTC 125 2.2551623E-4 22.798662 5 >>END_MODULE