FastQCFastQC Report
Thu 26 May 2016
SRR936831_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936831_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950763
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56620.5955217020435166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40340.4242908064365147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25020.26315706437881997No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9910.1042320746600362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57600.034.9182131
GGTATCA35700.034.2293781
TCAACGC69550.028.7803574
ATCAACG69800.028.6757623
CAACGCA70100.028.2772875
TATCAAC71450.028.0830542
AACGCAG71950.027.6133696
CGGGTCG700.008364473527.160387
GAGTACT38400.024.87605712-13
ACGCAGA80100.024.3290567
CGCAGAG80500.024.0373658
GTACATG46950.022.9892271
TACATGG47450.022.5465642
GCAGAGT82900.022.3665189
AGTACTT41700.021.82475712-13
GTACTTT43600.021.58218414-15
GTACACG1353.7693663E-421.1325341
AGAGTAC69400.020.88773510-11
ACATGGG48400.020.5300143
GTCCTAT2702.2708264E-819.371491
Produced by FastQC (version 0.11.5)