##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936831_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 950763 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79240988553404 33.0 31.0 34.0 30.0 34.0 2 31.87673373911269 33.0 31.0 34.0 30.0 34.0 3 31.885471984080155 33.0 31.0 34.0 30.0 34.0 4 35.4496767333184 37.0 35.0 37.0 33.0 37.0 5 35.481890860288 37.0 35.0 37.0 33.0 37.0 6 35.551624326987906 37.0 35.0 37.0 33.0 37.0 7 35.49266851991506 37.0 35.0 37.0 33.0 37.0 8 35.469977270886645 37.0 35.0 37.0 33.0 37.0 9 37.090825999749676 39.0 37.0 39.0 33.0 39.0 10-11 36.96846900857522 39.0 37.0 39.0 33.0 39.0 12-13 36.86426638394637 39.0 37.0 39.0 33.0 39.0 14-15 38.009482910041726 40.0 38.0 41.0 33.0 41.0 16-17 37.952905718880515 40.0 38.0 41.0 33.0 41.0 18-19 37.9338625924652 40.0 38.0 41.0 33.0 41.0 20-21 37.89906054400518 40.0 38.0 41.0 33.0 41.0 22-23 37.76254650212513 40.0 37.5 41.0 32.5 41.0 24-25 37.598555055255616 40.0 37.0 41.0 32.0 41.0 26-27 37.557936099743046 40.0 37.0 41.0 32.0 41.0 28-29 37.63116412817915 40.0 37.0 41.0 32.0 41.0 30-31 37.48550953286991 40.0 37.0 41.0 32.0 41.0 32-33 37.29387186922503 40.0 36.5 41.0 31.5 41.0 34-35 37.101411182387196 40.0 36.0 41.0 31.0 41.0 36-37 36.96241439769953 39.0 36.0 41.0 31.0 41.0 38-39 36.81092606674849 39.0 36.0 41.0 30.5 41.0 40-41 36.6104696964438 39.0 35.5 41.0 30.0 41.0 42-43 36.40479278221807 39.0 35.0 41.0 30.0 41.0 44-45 36.14565564709606 39.0 35.0 41.0 30.0 41.0 46-47 35.78039164334329 39.0 35.0 40.0 28.5 41.0 48-49 35.41728853562876 39.0 35.0 40.0 27.0 41.0 50-51 34.7701225226476 38.5 34.0 39.5 25.5 40.5 52-53 35.06654813029114 38.5 35.0 40.0 26.5 40.5 54-55 35.38327217192928 39.0 35.0 40.5 27.0 41.0 56-57 35.28778991189182 39.0 35.0 41.0 26.0 41.0 58-59 35.06810214532959 39.0 35.0 41.0 26.0 41.0 60-61 34.8041925274753 38.0 34.5 40.0 26.0 41.0 62-63 34.515235132204346 38.0 34.0 40.0 25.0 41.0 64-65 34.228195144320935 37.5 34.0 40.0 24.5 41.0 66-67 33.86956318241244 37.0 34.0 40.0 23.0 41.0 68-69 33.44334708018718 36.5 33.0 39.0 22.0 41.0 70-71 33.104623339360074 36.0 33.0 39.0 22.0 41.0 72-73 32.726724220441895 35.5 33.0 39.0 21.0 40.0 74-75 32.296699598112255 35.0 32.0 38.0 19.5 40.0 76-77 31.853526062751705 35.0 32.0 37.0 18.0 39.0 78-79 31.421427842690555 35.0 31.0 37.0 17.0 39.0 80-81 30.991554677664148 35.0 31.0 36.0 12.5 38.5 82-83 30.57071793917096 35.0 31.0 36.0 7.5 37.0 84-85 30.2136205342446 34.0 31.0 35.0 7.0 37.0 86-87 29.880023202417426 34.0 30.0 35.0 2.0 36.5 88-89 29.50744349538213 34.0 30.0 35.0 2.0 36.0 90-91 29.17028270978151 34.0 29.5 35.0 2.0 36.0 92-93 28.823829913448463 34.0 29.0 35.0 2.0 35.5 94-95 28.439033176511916 34.0 29.0 35.0 2.0 35.0 96-97 28.01537870110637 34.0 29.0 35.0 2.0 35.0 98-99 27.450904168546735 34.0 28.0 35.0 2.0 35.0 100-101 25.335713001031802 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5109104725723661 1101 2 0.4210790660178141 1101 3 0.5105593635068821 1101 4 0.4141001486917446 1101 5 0.2902964627637843 1101 6 0.31215829023611974 1101 7 0.4626881150489126 1101 8 0.557756249055771 1101 9 0.5941224154063676 1101 10-11 0.6513168717966167 1101 12-13 0.6721817904921963 1101 14-15 0.8490696658794121 1101 16-17 0.8426857238429477 1101 18-19 0.8877673696530692 1101 20-21 0.831699092754782 1101 22-23 0.934383649901946 1101 24-25 0.9734844032525842 1101 26-27 0.995618728674657 1101 28-29 0.8246016729818848 1101 30-31 0.6497695337630844 1101 32-33 0.7138448050128332 1101 34-35 0.7372844283603328 1101 36-37 0.8002207838236259 1101 38-39 0.8445053809430405 1101 40-41 0.8160523054683964 1101 42-43 0.8581147718637894 1101 44-45 0.83731526232215 1101 46-47 0.7656392577911646 1101 48-49 0.7868652939221406 1101 50-51 1.0371990102023076 1101 52-53 1.086079150639442 1101 54-55 1.120097684555084 1101 56-57 1.081168680346721 1101 58-59 0.8905155914355944 1101 60-61 1.028533893766145 1101 62-63 1.0128051345160003 1101 64-65 0.8288927609782988 1101 66-67 0.9312000604081945 1101 68-69 0.8797921195148675 1101 70-71 0.9626139624583558 1101 72-73 1.030505144113981 1101 74-75 0.8239585631841777 1101 76-77 0.8134433050755199 1101 78-79 1.0317230817021006 1101 80-81 0.941243007854144 1101 82-83 0.6821932390641159 1101 84-85 0.9295522032712888 1101 86-87 0.8523829322388679 1101 88-89 0.8017870031200403 1101 90-91 0.7621138933917351 1101 92-93 0.5822255043796716 1101 94-95 0.7671538738608952 1101 96-97 0.6447013906801651 1101 98-99 0.6866848961760859 1101 100-101 0.9561272323853522 1102 1 0.3423517431104379 1102 2 0.37010781931017434 1102 3 0.30069011730093465 1102 4 0.34060601223119136 1102 5 0.3637804085809222 1102 6 0.43010479719711014 1102 7 0.46937004155086015 1102 8 0.5270267557659167 1102 9 0.5074611837495269 1102 10-11 0.598562262490212 1102 12-13 0.3924752810227403 1102 14-15 0.586255323724636 1102 16-17 0.5225443090680812 1102 18-19 0.5448432037180382 1102 20-21 0.4559641905849574 1102 22-23 0.4944241773069393 1102 24-25 0.6847744603201917 1102 26-27 0.6219474317976932 1102 28-29 0.7705626506565295 1102 30-31 0.6114417399589982 1102 32-33 0.7702421773946213 1102 34-35 0.5758230900936852 1102 36-37 0.6021762115170972 1102 38-39 0.6820273300211781 1102 40-41 0.8260156191520736 1102 42-43 0.8305659351649268 1102 44-45 0.7793324609926628 1102 46-47 0.4633510663951981 1102 48-49 0.6096547081953148 1102 50-51 0.6482636972711688 1102 52-53 0.8400088195219197 1102 54-55 0.7244631994177482 1102 56-57 0.7877667300143543 1102 58-59 0.5704314010430167 1102 60-61 0.6597098156274299 1102 62-63 0.6025725526772874 1102 64-65 0.9474761831531424 1102 66-67 0.7474776535350074 1102 68-69 0.6733093025074695 1102 70-71 0.751410525859832 1102 72-73 0.5695776387834925 1102 74-75 0.5739750249927837 1102 76-77 0.6661885076341569 1102 78-79 0.7444511953356105 1102 80-81 0.6711211904704761 1102 82-83 0.6633797435166464 1102 84-85 0.5599344307891592 1102 86-87 0.6942562860580104 1102 88-89 0.824110783378412 1102 90-91 0.6435822083296507 1102 92-93 0.6773963152413316 1102 94-95 0.4003181470328485 1102 96-97 0.27730831404652534 1102 98-99 0.35290016959888604 1102 100-101 0.5692312239819906 1103 1 0.2979418458951777 1103 2 0.3951467455758255 1103 3 0.38108356766368345 1103 4 0.32192868065701674 1103 5 0.20685460535784017 1103 6 0.24200241020454882 1103 7 0.21490463701681506 1103 8 0.18187325773769913 1103 9 0.4104470668138944 1103 10-11 0.45382607989974844 1103 12-13 0.36688055397549135 1103 14-15 0.4256301104992417 1103 16-17 0.6179981416277371 1103 18-19 0.6090323328206608 1103 20-21 0.4954923379534151 1103 22-23 0.6221015201992302 1103 24-25 0.7209924603428277 1103 26-27 0.6819759373039602 1103 28-29 0.82524212944071 1103 30-31 0.8193334643076824 1103 32-33 0.7892279278752383 1103 34-35 0.8955306135747492 1103 36-37 0.7882247630396293 1103 38-39 0.7058083275360403 1103 40-41 0.9569198403276431 1103 42-43 1.0289423501326596 1103 44-45 1.0616024564815802 1103 46-47 0.9459684474780872 1103 48-49 1.1015719500652565 1103 50-51 1.1510358294604046 1103 52-53 0.9740707975744556 1103 54-55 1.0451651010244305 1103 56-57 1.0008296248350206 1103 58-59 1.0541814030641135 1103 60-61 0.9358025401749828 1103 62-63 0.9799790690069159 1103 64-65 1.250223496311115 1103 66-67 1.109102257198522 1103 68-69 1.3364778601094471 1103 70-71 1.2621469800852978 1103 72-73 1.1827479749479792 1103 74-75 1.0982564453204589 1103 76-77 1.1263050519842288 1103 78-79 1.1869470359246783 1103 80-81 1.211051939714185 1103 82-83 0.9848767434050778 1103 84-85 1.0932295250287218 1103 86-87 1.032988365019719 1103 88-89 1.2256984737856556 1103 90-91 1.2314731080720804 1103 92-93 1.2580961634167664 1103 94-95 1.099788377938765 1103 96-97 1.0062970234083934 1103 98-99 1.2711158025165226 1103 100-101 1.4498237925011104 1104 1 0.42415829708288655 1104 2 0.4251717056117208 1104 3 0.27516233667584444 1104 4 0.3224531218607112 1104 5 0.21184287320613748 1104 6 0.2749664845798421 1104 7 0.2836634595014189 1104 8 0.2715751395561341 1104 9 0.4655872449930314 1104 10-11 0.4030300697661673 1104 12-13 0.4297597455320812 1104 14-15 0.7198229291136329 1104 16-17 0.6849536716312059 1104 18-19 0.591146176759672 1104 20-21 0.518761728722879 1104 22-23 0.6950231531651809 1104 24-25 0.6050357991916755 1104 26-27 0.6077516148240392 1104 28-29 0.5376024887904407 1104 30-31 0.5197387143287173 1104 32-33 0.35589973612233194 1104 34-35 0.5165311651261035 1104 36-37 0.550572034730088 1104 38-39 0.4630967515014177 1104 40-41 0.395212929108709 1104 42-43 0.6675214977521122 1104 44-45 0.668078043322879 1104 46-47 0.6461278364713721 1104 48-49 0.5176847282948742 1104 50-51 0.457671272711373 1104 52-53 0.352844016484255 1104 54-55 0.4063513103708871 1104 56-57 0.3091917042771115 1104 58-59 0.5285730606161607 1104 60-61 0.6242984876747713 1104 62-63 0.599976544958345 1104 64-65 0.7240047080413916 1104 66-67 0.5357459269781728 1104 68-69 0.39145818187890313 1104 70-71 0.43935435404646483 1104 72-73 0.5584783130769964 1104 74-75 0.6431527256207303 1104 76-77 0.4803486712829219 1104 78-79 0.5884081795991314 1104 80-81 0.5174803174988192 1104 82-83 0.5035855523346022 1104 84-85 0.6776033272741877 1104 86-87 0.6151050134571463 1104 88-89 0.6389969378553566 1104 90-91 0.5493714169595378 1104 92-93 0.4929420562288378 1104 94-95 0.6739930689297822 1104 96-97 0.6442844218621815 1104 98-99 0.543639664123873 1104 100-101 0.7625426602316132 1105 1 0.29483100024289755 1105 2 0.3115382752270186 1105 3 0.3115693020168173 1105 4 0.24534657368817392 1105 5 0.23068506565238778 1105 6 0.2351723757502029 1105 7 0.31865479601559343 1105 8 0.22980240235192184 1105 9 0.3601813314652844 1105 10-11 0.3466747296544739 1105 12-13 0.21664053495094038 1105 14-15 0.364364424924176 1105 16-17 0.46391588257310445 1105 18-19 0.29865012741083063 1105 20-21 0.304772612703303 1105 22-23 0.3975454724387646 1105 24-25 0.42615895280214033 1105 26-27 0.29468118398710885 1105 28-29 0.3167308557360684 1105 30-31 0.3790935005870537 1105 32-33 0.3022948622128965 1105 34-35 0.31983751716342823 1105 36-37 0.3762865817644254 1105 38-39 0.45430267824033876 1105 40-41 0.4823167842328715 1105 42-43 0.47016119328521455 1105 44-45 0.5968448812267724 1105 46-47 0.7284207893226906 1105 48-49 0.6288644359212796 1105 50-51 0.5595990888523872 1105 52-53 0.6990430073532963 1105 54-55 0.7507256339330297 1105 56-57 0.7873150052430447 1105 58-59 0.8948190019890063 1105 60-61 0.6956729077801569 1105 62-63 0.5036035221893798 1105 64-65 0.556749635710176 1105 66-67 0.8329670059251484 1105 68-69 0.825804203026955 1105 70-71 0.5236142484586424 1105 72-73 0.5355137241225592 1105 74-75 0.6922205751961883 1105 76-77 0.4556122597444734 1105 78-79 0.7838975776265933 1105 80-81 0.718226241087379 1105 82-83 1.0281105849814622 1105 84-85 0.8592372454563275 1105 86-87 0.8624117110176499 1105 88-89 0.8264884903215268 1105 90-91 0.969501191404035 1105 92-93 0.879109441888609 1105 94-95 0.6931289560859781 1105 96-97 0.8759719469507239 1105 98-99 0.8986535612697502 1105 100-101 0.9662753269084448 1106 1 0.5351258405377344 1106 2 0.4008139145026597 1106 3 0.4494662899138042 1106 4 0.24675707509867095 1106 5 0.22584841081573614 1106 6 0.34597863655646677 1106 7 0.45089157825237436 1106 8 0.5278049503544722 1106 9 0.645421116705073 1106 10-11 0.6138168717966224 1106 12-13 0.5710995394099427 1106 14-15 0.7624625230222648 1106 16-17 0.5565926502498755 1106 18-19 0.60831661207731 1106 20-21 0.6190232269539138 1106 22-23 0.8080173079106032 1106 24-25 0.7387306153737967 1106 26-27 0.8918579061638354 1106 28-29 0.6899290539342644 1106 30-31 0.862485983979532 1106 32-33 0.8335147184327454 1106 34-35 0.8663158136417195 1106 36-37 0.7788869093647577 1106 38-39 0.8395811385188026 1106 40-41 0.7690284959445819 1106 42-43 0.8957094688334806 1106 44-45 0.8131134224953129 1106 46-47 0.6923438032457128 1106 48-49 0.8711726532295003 1106 50-51 0.9012953305486278 1106 52-53 0.9395152978255865 1106 54-55 0.8090316672390614 1106 56-57 1.0380680309960724 1106 58-59 1.2234430806130874 1106 60-61 0.9695376816449368 1106 62-63 1.2389820825679436 1106 64-65 1.1079917869523328 1106 66-67 1.0949175928757313 1106 68-69 1.0416427688655219 1106 70-71 1.012289287133683 1106 72-73 0.9571285207373634 1106 74-75 0.8968752298508491 1106 76-77 0.8824368115690255 1106 78-79 1.1486873674163895 1106 80-81 1.0418111896723268 1106 82-83 0.9396607715316492 1106 84-85 1.003956965176048 1106 86-87 0.8548450534509939 1106 88-89 0.8632047520377917 1106 90-91 1.0544975514003951 1106 92-93 1.1975528853320547 1106 94-95 1.104626817583835 1106 96-97 0.8610433820221566 1106 98-99 1.0116984243146128 1106 100-101 1.306708942342059 1107 1 0.19071308056663483 1107 2 0.29178954280576974 1107 3 0.28056967001342414 1107 4 0.3376471553237579 1107 5 0.23776240648130198 1107 6 0.23963857344099182 1107 7 0.24012582790067682 1107 8 0.4548894718555516 1107 9 0.4197452868887126 1107 10-11 0.39596869111908717 1107 12-13 0.30575411528158725 1107 14-15 0.41541108011135464 1107 16-17 0.44607118718762706 1107 18-19 0.45118095183625684 1107 20-21 0.46890726178148867 1107 22-23 0.23982313278418133 1107 24-25 0.42805231416852507 1107 26-27 0.3160670617092478 1107 28-29 0.16946690420611787 1107 30-31 0.2829452821050822 1107 32-33 0.24957589481387288 1107 34-35 0.33562306431660005 1107 36-37 0.37140905681162195 1107 38-39 0.22099534330188675 1107 40-41 0.466090394731701 1107 42-43 0.4439718923154814 1107 44-45 0.6299388283734189 1107 46-47 0.5237399478789868 1107 48-49 0.5463741664799429 1107 50-51 0.4687604459434809 1107 52-53 0.4407338826702727 1107 54-55 0.7125324846984782 1107 56-57 0.689251446075346 1107 58-59 0.7196124277861955 1107 60-61 0.7625873755729273 1107 62-63 0.7829851622987576 1107 64-65 0.8230824679150288 1107 66-67 0.8426391623978091 1107 68-69 1.0323978732341743 1107 70-71 0.9720645808487447 1107 72-73 0.8441052516601388 1107 74-75 1.0428183498843069 1107 76-77 0.9035244128009232 1107 78-79 0.8992833523599195 1107 80-81 1.0778411885316892 1107 82-83 0.9316106973899814 1107 84-85 0.8002033056193767 1107 86-87 0.8019516273455203 1107 88-89 0.73747664284042 1107 90-91 0.5207450324847613 1107 92-93 0.5448448133956276 1107 94-95 0.6191490790948073 1107 96-97 0.7988195122073201 1107 98-99 0.9038413086155899 1107 100-101 1.3400261165939895 1108 1 0.020557887222683036 1108 2 0.05708544652825509 1108 3 0.0641432216441018 1108 4 0.08335976088929442 1108 5 0.1925959324356441 1108 6 0.18363243673926632 1108 7 0.03891449887390763 1108 8 -0.22346060543544866 1108 9 0.033946744209821134 1108 10-11 -0.26930377785534887 1108 12-13 -0.30317452372722187 1108 14-15 0.09123282464639715 1108 16-17 0.0589145935810933 1108 18-19 0.1342798406507555 1108 20-21 0.044436290302016346 1108 22-23 0.054872753315933664 1108 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1.0533251545783457 1108 92-93 1.0021739216816279 1108 94-95 1.1624451400193294 1108 96-97 0.789704705231042 1108 98-99 0.9142857828184425 1108 100-101 1.0443574693757363 1109 1 0.06632013643791623 1109 2 0.10363625556029987 1109 3 0.08211631962265997 1109 4 0.16208684336961454 1109 5 0.1842857251354033 1109 6 0.12426146483929301 1109 7 0.1693407761133372 1109 8 0.2867770240324248 1109 9 0.27007946489283086 1109 10-11 0.20384955153518547 1109 12-13 0.271901678447378 1109 14-15 0.27684160798958857 1109 16-17 0.03711849695218916 1109 18-19 0.1173629578883677 1109 20-21 0.23916582944946185 1109 22-23 0.06492461628849355 1109 24-25 0.18575973004997337 1109 26-27 0.1578561236326408 1109 28-29 -0.08049578266704316 1109 30-31 0.12683536009361518 1109 32-33 0.35340479567576466 1109 34-35 0.1366366692566956 1109 36-37 0.3618191731793772 1109 38-39 0.33086159009337024 1109 40-41 0.43324418221909866 1109 42-43 0.45854369343241075 1109 44-45 0.4226648188114126 1109 46-47 0.11322912707220922 1109 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-0.2662550257847265 2312 98-99 -0.0061495469231864774 2312 100-101 0.031344883004919666 2313 1 -0.5468667830667293 2313 2 -0.6558946181927148 2313 3 -0.5055825537863541 2313 4 -0.5476009791278003 2313 5 -0.3818369958828285 2313 6 -1.319561634575912 2313 7 -0.7485900804398113 2313 8 -0.5442644276359587 2313 9 -0.545891682337988 2313 10-11 -0.5930087860981175 2313 12-13 -0.45979659296645536 2313 14-15 -0.58112300329352 2313 16-17 -0.4468114566081809 2313 18-19 -0.6026109198206129 2313 20-21 -0.673293858373043 2313 22-23 -0.5461144703988055 2313 24-25 -0.5436208599053032 2313 26-27 -0.5632980946336161 2313 28-29 -0.5283823495745139 2313 30-31 -0.4566801842820283 2313 32-33 -0.38266600325784594 2313 34-35 -0.3937893498351528 2313 36-37 -0.2854347612891459 2313 38-39 -0.46306040853064445 2313 40-41 -0.3329726005466469 2313 42-43 -0.5413095702414736 2313 44-45 -0.5312820121139339 2313 46-47 -0.7138837685246315 2313 48-49 -0.5556041609650819 2313 50-51 -0.3413606702488181 2313 52-53 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2316 50-51 -1.462194066889957 2316 52-53 -1.4361268044908826 2316 54-55 -1.9002359512086926 2316 56-57 -1.4299160826577832 2316 58-59 -1.4405117097851203 2316 60-61 -1.5696882963801144 2316 62-63 -1.3822002954851058 2316 64-65 -1.4840440448063674 2316 66-67 -1.6376791598711335 2316 68-69 -1.1625247725999444 2316 70-71 -1.7215304343303828 2316 72-73 -1.3365326871848175 2316 74-75 -1.6908460942446126 2316 76-77 -1.9566614458317666 2316 78-79 -2.1522556656860026 2316 80-81 -2.086985437103511 2316 82-83 -2.005753533178268 2316 84-85 -1.658918323507553 2316 86-87 -1.4394617559828404 2316 88-89 -2.0902192104508366 2316 90-91 -1.8438525780698285 2316 92-93 -2.2963847590097473 2316 94-95 -2.2603707329795313 2316 96-97 -2.1590734910970255 2316 98-99 -2.432611884314401 2316 100-101 -2.3248275542637202 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2557.0 3 707.0 4 716.0 5 742.0 6 754.0 7 978.0 8 1235.0 9 1410.0 10 1794.0 11 2240.0 12 3060.0 13 3911.0 14 4357.0 15 5487.0 16 7274.0 17 9781.0 18 8641.0 19 5430.0 20 4981.0 21 5223.0 22 5521.0 23 6126.0 24 7058.0 25 8244.0 26 9323.0 27 11137.0 28 13504.0 29 16234.0 30 19962.0 31 24981.0 32 31794.0 33 41727.0 34 57363.0 35 82895.0 36 124577.0 37 189481.0 38 190702.0 39 38821.0 40 35.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.995857068876234 20.02237772248256 15.170011746725443 21.81175346191577 2 14.74513016758247 21.305951210060424 41.27305390987151 22.6758647124856 3 18.58570845105412 26.510861189825558 30.48943776131909 24.413992597801233 4 12.173895638176386 17.880800980999457 38.882954291661925 31.062349089162232 5 13.030525138535223 38.165269203793684 34.509961267004925 14.294244390666169 6 29.343289837689042 38.01928607609334 18.457724697950812 14.179699388266812 7 26.728678447641226 34.15597942910825 21.790063581032904 17.325278542217617 8 25.350696338353256 35.2669630465777 20.581205077067903 18.80113553800114 9 26.037458027713296 17.511870205048048 21.282108536015066 35.16856323122359 10-11 23.66178879597456 27.92902611260683 28.054873807408796 20.354311284009814 12-13 25.140726872988868 25.65088153040079 28.44209613019526 20.766295466415084 14-15 22.308235416762095 26.63262292155986 27.354723082617422 23.704418579060622 16-17 20.834691537915894 30.115805920420357 27.660874176454076 21.388628365209673 18-19 20.694923695912443 28.307168572382267 30.69932514607444 20.298582585630847 20-21 21.75010198576147 27.383348028055956 30.626258372702033 20.240291613480544 22-23 21.326412499190692 27.546267418546762 30.507940167525454 20.61937991473709 24-25 21.184857695495992 27.692206476401005 30.631350412505427 20.491585415597573 26-27 20.958265972853727 27.830245276346368 30.471775959044827 20.73971279175508 28-29 20.732430068181745 27.96935874209573 30.660942922919993 20.637268266802533 30-31 20.979288134879706 27.690069250036835 30.654612810204384 20.676029804879075 32-33 20.789524645478522 27.878165013250484 30.438486813716516 20.89382352755448 34-35 21.151690743977866 27.915771682472062 30.30247220857017 20.630065364979906 36-37 21.198171716757123 27.63089348239967 30.254341015027663 20.916593785815547 38-39 20.85943000758825 28.00527914977903 30.37337144649335 20.76191939613936 40-41 21.13211281127102 27.81880805409821 30.264448786074595 20.784630348556174 42-43 20.96599572518891 27.943906394163687 29.991573537856187 21.098524342791215 44-45 21.078780135096164 28.14943723964308 29.72962874866598 21.042153876594767 46-47 21.210941384388494 28.315612081633684 29.422925050917144 21.05052148306068 48-49 21.274675708726136 28.49464232719374 29.118072963694953 21.11260900038517 50-51 21.214910988594756 28.651624510752917 28.924655948823702 21.208808551828625 52-53 21.43300266091932 28.87499993422683 28.62569331497297 21.066304089880877 54-55 21.176465016368443 28.762202659260126 28.709842588260926 21.351489736110505 56-57 21.254293425894694 29.042266728902817 28.50913729437996 21.194302550822535 58-59 21.492207244047414 28.6443120302216 28.633312597560757 21.23016812817023 60-61 21.385024320599335 28.703990704336974 28.953748498216804 20.957236476846887 62-63 21.335681971761606 28.714525700644383 28.94103914093304 21.008753186660968 64-65 21.211354309165525 28.756918867725982 28.801273282121436 21.230453540987057 66-67 21.180927638628944 28.958022362528972 28.49032929044971 21.37072070839237 68-69 21.126842096122907 28.846976628409553 28.768038109411947 21.258143166055596 70-71 21.23198385635693 28.833247235306438 28.618510690008176 21.316258218328464 72-73 21.184504618809477 28.833985347950662 28.55292733258953 21.42858270065033 74-75 21.088771068516674 29.080576718914237 28.402869916278327 21.42778229629076 76-77 21.17774520090378 28.97521760498237 28.44018980647863 21.406847387635217 78-79 21.159955285528426 29.010039704092932 28.231852113832435 21.598152896546207 80-81 21.15842869311889 29.165809485275506 28.252312811314688 21.423449010290913 82-83 21.227182160641377 29.122420346097535 28.359536417497917 21.290861075763175 84-85 21.151945434163643 29.077991042529565 28.154666357909363 21.61539716539743 86-87 21.131768021082294 28.947522162040396 28.322937626284023 21.597772190593282 88-89 21.27162365625362 28.806079366583486 28.242837739663283 21.679459237499604 90-91 21.09835016430991 28.77439123231379 28.555083291755874 21.572175311620427 92-93 21.260210575519043 28.836223368808252 28.347000072641286 21.55656598303142 94-95 20.930885100500277 28.810763829481452 28.376028600503172 21.8823224695151 96-97 20.973310897611398 28.72874538263576 28.46328642610743 21.834657293645414 98-99 20.889494684773126 28.957242233617176 28.142999195237152 22.01026388637254 100-101 20.75122118207792 29.009492962567972 28.16805572013535 22.07123013521876 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 606.0 1 408.0 2 242.0 3 294.0 4 292.5 5 316.0 6 494.0 7 889.5 8 1458.0 9 1769.5 10 1677.0 11 1509.0 12 1525.0 13 1661.5 14 1743.0 15 1762.5 16 1806.5 17 1922.5 18 1996.0 19 2077.5 20 2339.5 21 2731.5 22 3159.0 23 3803.5 24 4494.0 25 5512.5 26 7070.5 27 8906.0 28 10208.5 29 11593.0 30 13998.5 31 16218.5 32 18376.5 33 20903.5 34 23991.5 35 26554.0 36 28972.5 37 31395.0 38 32529.5 39 32841.5 40 32876.5 41 33633.5 42 34418.0 43 34718.0 44 35441.0 45 35985.0 46 35036.0 47 34535.0 48 36276.0 49 35922.5 50 32666.0 51 29766.0 52 27392.5 53 25724.0 54 24178.0 55 21858.5 56 19364.0 57 17110.0 58 15102.0 59 13338.5 60 11191.5 61 8856.5 62 6811.0 63 5105.5 64 3866.0 65 2865.5 66 2036.0 67 1514.0 68 1116.5 69 729.0 70 492.5 71 348.0 72 224.0 73 154.0 74 106.5 75 79.0 76 61.5 77 31.0 78 20.0 79 13.5 80 6.0 81 8.0 82 10.0 83 6.0 84 4.5 85 4.0 86 2.5 87 1.5 88 1.0 89 2.5 90 2.0 91 1.5 92 1.5 93 0.5 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07478204347455675 2 0.12000887708082876 3 0.0815134791740949 4 0.16060784864366828 5 0.12463673912426125 6 0.12263834415096085 7 0.13315621243148923 8 0.11369815611251173 9 0.13957211208261155 10-11 0.12647736607335372 12-13 0.11054279562835323 14-15 0.11595949779282534 16-17 0.13531237542899754 18-19 0.11317226269848533 20-21 0.09208393679602593 22-23 0.0922942941616365 24-25 0.08156606851549755 26-27 0.06847132250623972 28-29 0.08435330360983757 30-31 0.061319172075480426 32-33 0.0640538178284178 34-35 0.058900062370958906 36-37 0.06121399339267515 38-39 0.06415899651122309 40-41 0.08277562336775832 42-43 0.0819867832467187 44-45 0.06573667675330234 46-47 0.04675192450694863 48-49 0.05648095266643738 50-51 0.033972714546106654 52-53 0.05548175517978718 54-55 0.06431676453543102 56-57 0.065053015315068 58-59 0.07509757952297261 60-61 0.06757730370239481 62-63 0.07457168610894618 64-65 0.04869773013884638 66-67 0.08214455127092662 68-69 0.06904980526166878 70-71 0.031132890110363992 72-73 0.026031723994307733 74-75 0.03702289634745988 76-77 0.055955059252410955 78-79 0.07730633186188356 80-81 0.048224426066222605 82-83 0.07194221903881408 84-85 0.07551829425419374 86-87 0.08114535378427642 88-89 0.10444243202564678 90-91 0.07667525976505186 92-93 0.09360902769670255 94-95 0.10407430663582828 96-97 0.11432922820934345 98-99 0.1488278361694765 100-101 0.14467327819866782 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 950763.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.37763031627532 #Duplication Level Percentage of deduplicated Percentage of total 1 84.2230021066076 56.74746300065884 2 9.940492790833092 13.395336968447038 3 2.5614772077416976 5.177587931003558 4 1.0648481933024863 2.8698779164515433 5 0.5457699074095612 1.8386341529594612 6 0.3306601216338304 1.3367457261467315 7 0.21995860866749437 1.0374202873776495 8 0.15485790279714962 0.8347166820976035 9 0.12901321238978977 0.782334407728293 >10 0.7530419855416247 9.51303574245892 >50 0.054760792731270644 2.513054838819228 >100 0.0210191549439715 2.322707772228614 >500 6.274373716565279E-4 0.34190102997919464 >1k 3.1371868582826397E-4 0.690777475098614 >5k 1.5685934291413198E-4 0.5984060685447296 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5662 0.5955217020435166 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4034 0.4242908064365147 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2502 0.26315706437881997 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 991 0.1042320746600362 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.0517868280528375E-4 0.0 0.0 0.0 0.0 7 2.103573656105675E-4 0.0 0.0 0.0 0.0 8 2.103573656105675E-4 0.0 0.0 1.0517868280528375E-4 0.0 9 2.103573656105675E-4 0.0 0.0 1.0517868280528375E-4 0.0 10-11 2.103573656105675E-4 0.0 0.0 2.103573656105675E-4 0.0 12-13 2.103573656105675E-4 0.0 0.0 2.103573656105675E-4 0.0 14-15 2.103573656105675E-4 0.0 0.0 2.103573656105675E-4 0.0 16-17 2.103573656105675E-4 0.0 0.0 2.103573656105675E-4 0.0 18-19 2.103573656105675E-4 0.0 0.0 2.103573656105675E-4 0.0 20-21 2.103573656105675E-4 0.0 0.0 2.629467070132094E-4 0.0 22-23 2.103573656105675E-4 0.0 0.0 5.258934140264188E-4 0.0 24-25 3.155360484158513E-4 0.0 0.0 8.414294624422701E-4 0.0 26-27 3.6812538981849315E-4 0.0 0.0 0.0010517868280528375 0.0 28-29 4.20714731221135E-4 0.0 0.0 0.002103573656105675 0.0 30-31 5.258934140264188E-4 0.0 0.0 0.004417504677821918 0.0 32-33 5.258934140264188E-4 0.0 0.0 0.007467686479175147 0.0 34-35 5.258934140264188E-4 0.0 0.0 0.010044564207904599 0.0 36-37 5.258934140264188E-4 0.0 0.0 0.013252514033465754 0.0 38-39 6.310720968317026E-4 0.0 0.0 0.017775197394092956 0.0 40-41 6.310720968317026E-4 0.0 0.0 0.020562432488432973 0.0 42-43 7.362507796369863E-4 0.0 0.0 0.029502620526882094 0.0 44-45 7.362507796369863E-4 0.0 0.0 0.03991531012460518 0.0 46-47 7.362507796369863E-4 0.0 0.0 0.0488554981630543 0.0 48-49 7.362507796369863E-4 0.0 0.0 0.06263390561054648 0.0 50-51 7.362507796369863E-4 0.0 0.0 0.07777963593450735 0.0 52-53 7.362507796369863E-4 0.0 0.0 0.10880734736206604 0.0 54-55 7.362507796369863E-4 0.0 0.0 0.13494425003917904 0.0 56-57 7.362507796369863E-4 0.0 0.0 0.18637662593096282 0.0 58-59 7.362507796369863E-4 0.0 0.0 0.24080659428269716 0.0 60-61 7.362507796369863E-4 0.0 0.0 0.2976030829975504 0.0 62-63 7.362507796369863E-4 0.0 0.0 0.3825874587042197 0.0 64-65 7.362507796369863E-4 0.0 0.0 0.4228708942186433 0.0 66-67 9.466081452475538E-4 0.0 0.0 0.5320989563119305 0.0 68-69 9.466081452475538E-4 0.0 0.0 0.6714081216875288 0.0 70-71 9.466081452475538E-4 0.0 0.0 0.7624928609969046 0.0 72-73 9.991974866501957E-4 0.0 0.0 0.9243628538342363 0.0 74-75 0.0010517868280528375 0.0 0.0 1.0857595425989444 0.0 76-77 0.0010517868280528375 0.0 0.0 1.176528745859904 0.0 78-79 0.0010517868280528375 0.0 0.0 1.2746078675758312 0.0 80-81 0.0011569655108581212 0.0 0.0 1.4719230765185436 0.0 82-83 0.0011569655108581212 0.0 0.0 1.6221708249058913 0.0 84-85 0.001209554852260763 0.0 0.0 1.8804896698756681 0.0 86-87 0.0012621441936634052 0.0 0.0 2.2707025830832714 0.0 88-89 0.0012621441936634052 0.0 0.0 2.7734040975511247 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5760 0.0 34.918213 1 GGTATCA 3570 0.0 34.229378 1 TCAACGC 6955 0.0 28.780357 4 ATCAACG 6980 0.0 28.675762 3 CAACGCA 7010 0.0 28.277287 5 TATCAAC 7145 0.0 28.083054 2 AACGCAG 7195 0.0 27.613369 6 CGGGTCG 70 0.0083644735 27.16038 7 GAGTACT 3840 0.0 24.876057 12-13 ACGCAGA 8010 0.0 24.329056 7 CGCAGAG 8050 0.0 24.037365 8 GTACATG 4695 0.0 22.989227 1 TACATGG 4745 0.0 22.546564 2 GCAGAGT 8290 0.0 22.366518 9 AGTACTT 4170 0.0 21.824757 12-13 GTACTTT 4360 0.0 21.582184 14-15 GTACACG 135 3.7693663E-4 21.132534 1 AGAGTAC 6940 0.0 20.887735 10-11 ACATGGG 4840 0.0 20.530014 3 GTCCTAT 270 2.2708264E-8 19.37149 1 >>END_MODULE