FastQCFastQC Report
Thu 26 May 2016
SRR936831_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936831_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950763
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78890.8297546286508836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60030.6313876328801183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41560.4371226057387593No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15260.16050266996086301No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11260.11843119683874952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36300.042.925391
GTATCAA60550.037.345631
TAGACCG600.00395026931.6672275
ATCAACG76000.029.6880263
TATCAAC76300.029.57132
TCAACGC76700.029.4170784
CAACGCA77850.028.9215155
AACGCAG79850.028.137636
CGATCGT350.00838789827.14619324-25
ACGCAGA89600.024.9684377
CGCAGAG90500.024.6676468
GAGTACT56250.023.5591812-13
GCAGAGT95100.023.1248539
GTACATG41650.023.0400471
GTGTACG1050.002194376422.6218281
AGAGTAC80300.021.88591210-11
AGTACTT58700.021.8476212-13
GTACTTT61150.021.5548514-15
TACATGG43250.021.5263942
TAGGACA3550.021.408834
Produced by FastQC (version 0.11.5)