##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936831_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 950763 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15562553443918 33.0 31.0 34.0 31.0 34.0 2 32.15820030859425 33.0 31.0 34.0 30.0 34.0 3 32.3263021383878 33.0 31.0 34.0 30.0 34.0 4 36.060175879793384 37.0 35.0 37.0 35.0 37.0 5 35.99876940941118 37.0 35.0 37.0 35.0 37.0 6 36.04715160350161 37.0 35.0 37.0 35.0 37.0 7 35.986206867536914 37.0 35.0 37.0 35.0 37.0 8 35.969348828256884 37.0 35.0 37.0 35.0 37.0 9 37.6566610185714 39.0 37.0 39.0 35.0 39.0 10-11 37.58780789744658 39.0 37.0 39.0 35.0 39.0 12-13 37.49485360705034 39.0 37.0 39.0 35.0 39.0 14-15 38.690007919954816 40.0 38.0 41.0 34.5 41.0 16-17 38.61278467925235 40.0 38.0 41.0 34.0 41.0 18-19 38.70440004501647 40.0 38.0 41.0 34.5 41.0 20-21 38.80449018314764 40.0 38.0 41.0 35.0 41.0 22-23 38.732210866430435 40.0 38.0 41.0 34.5 41.0 24-25 38.632523036761 40.0 38.0 41.0 34.0 41.0 26-27 38.47452940427846 40.0 38.0 41.0 34.0 41.0 28-29 38.30003008110329 40.0 38.0 41.0 34.0 41.0 30-31 38.11906857965655 40.0 37.0 41.0 34.0 41.0 32-33 38.046545774288646 40.0 37.0 41.0 33.5 41.0 34-35 37.94815216831114 40.0 37.0 41.0 33.0 41.0 36-37 37.77285927197419 40.0 37.0 41.0 33.0 41.0 38-39 37.60853966761432 40.0 37.0 41.0 33.0 41.0 40-41 37.4250964751468 40.0 36.5 41.0 33.0 41.0 42-43 37.177064105355385 40.0 36.0 41.0 32.0 41.0 44-45 36.77156662596252 40.0 36.0 41.0 31.0 41.0 46-47 36.4094916398724 39.5 36.0 41.0 30.0 41.0 48-49 36.00728520146451 39.0 35.5 41.0 29.5 41.0 50-51 35.73076150418138 39.0 35.0 41.0 28.0 41.0 52-53 35.49176871628366 39.0 35.0 40.0 27.5 41.0 54-55 35.27093134671837 39.0 35.0 40.0 27.0 41.0 56-57 35.01180735893172 38.5 35.0 40.0 26.0 41.0 58-59 34.71652872482417 38.0 34.0 40.0 26.0 41.0 60-61 34.40581196365446 38.0 34.0 40.0 25.5 41.0 62-63 34.056923754921044 37.5 34.0 40.0 24.5 41.0 64-65 33.68743104222609 37.0 33.0 40.0 23.0 41.0 66-67 33.331448005444045 36.5 33.0 39.0 22.0 40.5 68-69 32.90833151900105 36.0 32.5 39.0 21.5 40.0 70-71 32.44614956619053 35.5 32.0 38.5 20.0 40.0 72-73 32.11839228072611 35.0 31.5 38.0 19.5 40.0 74-75 31.67489952806325 35.0 31.0 37.0 18.0 39.0 76-77 30.269374702212854 33.5 29.5 36.0 16.0 38.5 78-79 30.964466433801064 34.5 31.0 36.0 16.0 39.0 80-81 30.774442737043827 34.5 31.0 36.0 12.0 38.0 82-83 30.43471033264862 34.0 31.0 35.5 7.5 37.0 84-85 30.0407062538193 34.0 30.0 35.0 7.0 37.0 86-87 29.676246866989985 34.0 30.0 35.0 2.0 36.0 88-89 29.314331226604317 34.0 30.0 35.0 2.0 36.0 90-91 28.893408767484644 34.0 29.0 35.0 2.0 35.5 92-93 28.45356518922171 34.0 29.0 35.0 2.0 35.0 94-95 27.99329801433165 34.0 29.0 35.0 2.0 35.0 96-97 27.378614333961252 33.0 27.0 35.0 2.0 35.0 98-99 26.70048319086881 33.0 25.5 35.0 2.0 35.0 100-101 23.769153826978965 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.509899265171299 1101 2 0.36957548299374565 1101 3 0.4128139694413875 1101 4 0.28672644657024193 1101 5 0.3520379317559943 1101 6 0.3534440200051421 1101 7 0.38226119881947795 1101 8 0.3425008346660974 1101 9 0.41778363763394566 1101 10-11 0.49752688483653884 1101 12-13 0.5150902757680882 1101 14-15 0.6249579071038696 1101 16-17 0.6712612843076613 1101 18-19 0.6675591754796102 1101 20-21 0.6082527727575808 1101 22-23 0.4401307489486399 1101 24-25 0.5772453577454755 1101 26-27 0.7064971308386774 1101 28-29 0.5591075196964965 1101 30-31 0.6278967871856267 1101 32-33 0.6420158759225885 1101 34-35 0.7501964143382054 1101 36-37 0.7114918818186879 1101 38-39 0.642927771815387 1101 40-41 0.595494825551981 1101 42-43 0.648178441355661 1101 44-45 0.7345345653808693 1101 46-47 0.8241232699091512 1101 48-49 0.7206366672743982 1101 50-51 0.8815208922147733 1101 52-53 0.7961395682075079 1101 54-55 0.8624337960057673 1101 56-57 0.7837931362038688 1101 58-59 0.8519861964504472 1101 60-61 1.0893746511162448 1101 62-63 1.025595053099991 1101 64-65 0.8524687703732425 1101 66-67 0.9087545811989486 1101 68-69 0.9084028089435421 1101 70-71 0.8390694185541392 1101 72-73 0.9999599488090141 1101 74-75 0.7652476730505313 1101 76-77 0.6596199115385168 1101 78-79 0.7171461492582694 1101 80-81 0.6990190794404505 1101 82-83 0.976037040851768 1101 84-85 0.9402850902996143 1101 86-87 0.7526450309067982 1101 88-89 0.8080847197461694 1101 90-91 0.8807095844638866 1101 92-93 1.0684488139746868 1101 94-95 1.2625766663621683 1101 96-97 1.4237356813208315 1101 98-99 1.496330331335919 1101 100-101 1.3246477755434682 1102 1 0.1957867918237497 1102 2 0.09940819966249848 1102 3 0.16353593733302318 1102 4 0.19926720914392604 1102 5 0.2531353856629295 1102 6 0.1570867830805227 1102 7 0.19341916947042392 1102 8 0.2950563344779624 1102 9 0.30501632921804855 1102 10-11 0.25509618121180466 1102 12-13 0.3840131247584253 1102 14-15 0.4437953114685982 1102 16-17 0.39533863913018763 1102 18-19 0.3989863358960193 1102 20-21 0.2803868502691813 1102 22-23 0.28164821414367935 1102 24-25 0.30519986044208025 1102 26-27 0.30068924798404595 1102 28-29 0.2758174312730688 1102 30-31 0.3820110634931595 1102 32-33 0.4202082909483025 1102 34-35 0.2916809559160356 1102 36-37 0.4373071332924141 1102 38-39 0.4736184622706716 1102 40-41 0.3817327535590067 1102 42-43 0.2947042158445612 1102 44-45 0.39422665117041333 1102 46-47 0.4296093636003562 1102 48-49 0.16730079934513498 1102 50-51 0.21604561628350893 1102 52-53 0.2672522296900155 1102 54-55 0.4283237527347339 1102 56-57 0.316734783011853 1102 58-59 0.2071438378652175 1102 60-61 0.06999243927629095 1102 62-63 0.25431777353144724 1102 64-65 0.2512121856497984 1102 66-67 0.2292746175717113 1102 68-69 0.31636052561229633 1102 70-71 0.11816801694872225 1102 72-73 0.2271423142925144 1102 74-75 0.11135210362873238 1102 76-77 0.18877220678497508 1102 78-79 0.2137056312600265 1102 80-81 0.1284621999722475 1102 82-83 0.12436185585239556 1102 84-85 0.15124943872555008 1102 86-87 -0.03315313164180367 1102 88-89 0.11246081720007695 1102 90-91 0.17049268053813904 1102 92-93 0.24694302882480557 1102 94-95 0.3317903406378484 1102 96-97 0.7671109792011812 1102 98-99 0.844833482596421 1102 100-101 0.9187152532808334 1103 1 0.03278962297187604 1103 2 -0.1733392105079652 1103 3 0.03797346694204862 1103 4 0.08584181094803967 1103 5 0.06833627429742961 1103 6 0.16163101027085958 1103 7 0.24661597387867573 1103 8 0.2401133050686255 1103 9 0.19916518196438915 1103 10-11 0.2515916046471105 1103 12-13 0.2761687748472781 1103 14-15 0.3086209234153685 1103 16-17 0.3249823445549609 1103 18-19 0.35573169586898246 1103 20-21 0.2608376188507151 1103 22-23 0.2861564658652398 1103 24-25 0.31555271107354343 1103 26-27 0.2779964073448653 1103 28-29 0.3996470158817118 1103 30-31 0.39498850647002115 1103 32-33 0.5200191974169357 1103 34-35 0.30379579607985363 1103 36-37 0.4266260570357332 1103 38-39 0.3106689912313314 1103 40-41 0.3582480483880772 1103 42-43 0.6319984887116235 1103 44-45 0.5932756861385613 1103 46-47 0.3358973096355342 1103 48-49 0.21658921265719044 1103 50-51 0.0067848030277133375 1103 52-53 0.0869068005147966 1103 54-55 0.27945645451668355 1103 56-57 0.1832455829284214 1103 58-59 0.2329673527251046 1103 60-61 0.17007134277633895 1103 62-63 0.13706078580191416 1103 64-65 0.15862668934193636 1103 66-67 0.3340982407622235 1103 68-69 0.18771992943814553 1103 70-71 0.1816412746446403 1103 72-73 0.3498744449942208 1103 74-75 0.3447407340871038 1103 76-77 0.275959361683217 1103 78-79 0.24938602034482926 1103 80-81 0.13157630122419306 1103 82-83 0.3180440784734486 1103 84-85 0.5222224094261598 1103 86-87 0.18391476255636263 1103 88-89 0.5421029057498536 1103 90-91 0.8758564208227391 1103 92-93 0.8546818776421432 1103 94-95 0.5395046129551666 1103 96-97 0.7114379307288843 1103 98-99 0.6304471755874346 1103 100-101 0.5110460917760413 1104 1 0.18580118685367353 1104 2 0.10060793404522883 1104 3 0.06150414348054767 1104 4 0.11617713547524744 1104 5 0.16955062211132343 1104 6 0.1335065653278349 1104 7 0.11666112630316405 1104 8 0.13733404713890707 1104 9 0.27894661805454035 1104 10-11 0.1894775737415344 1104 12-13 0.29286629099651407 1104 14-15 0.36406912900379496 1104 16-17 0.3017045402902525 1104 18-19 0.46254195285452226 1104 20-21 0.36907583294612323 1104 22-23 0.2908423153010702 1104 24-25 0.20818716340174603 1104 26-27 0.16730659421794059 1104 28-29 0.3656317220170209 1104 30-31 0.30858705187017677 1104 32-33 0.38907669898277675 1104 34-35 0.41937244769570725 1104 36-37 0.4293127665177465 1104 38-39 0.38497137225048306 1104 40-41 0.4232867037245711 1104 42-43 0.41659758566313343 1104 44-45 0.670412012806537 1104 46-47 0.8869835853551251 1104 48-49 0.7360622474049237 1104 50-51 0.4846096340566888 1104 52-53 0.4655150214344843 1104 54-55 0.3405225378476828 1104 56-57 0.38626322322345175 1104 58-59 0.31232203763971 1104 60-61 0.31885933204445394 1104 62-63 0.5109784831217468 1104 64-65 0.45592689220065097 1104 66-67 0.6427239430553655 1104 68-69 0.4830424934975639 1104 70-71 0.4246907927383319 1104 72-73 0.4211891003681103 1104 74-75 0.503706248104919 1104 76-77 0.365652362590005 1104 78-79 0.41597229139025416 1104 80-81 0.6955020381061523 1104 82-83 0.5341634005326981 1104 84-85 0.6336362505606736 1104 86-87 0.4577347698480594 1104 88-89 0.8242807857360184 1104 90-91 0.858000646827918 1104 92-93 0.9160489771363984 1104 94-95 0.9089757780372913 1104 96-97 1.0130168633367873 1104 98-99 1.0687809295955262 1104 100-101 1.0704780873636324 1105 1 0.1659061649281952 1105 2 -0.053943571775313615 1105 3 0.09118966656708949 1105 4 0.06263628498007989 1105 5 0.2042401339581943 1105 6 0.08489958271070464 1105 7 0.1762140802723593 1105 8 0.16778683495209634 1105 9 0.1476965862968953 1105 10-11 0.2061068934165391 1105 12-13 0.21167748798029606 1105 14-15 0.3894406934616583 1105 16-17 0.36555468485105536 1105 18-19 0.1730351872056275 1105 20-21 0.191396630901437 1105 22-23 0.378432443500337 1105 24-25 0.21915244340255668 1105 26-27 0.21066111625266615 1105 28-29 0.28547898044296005 1105 30-31 0.25477232343616407 1105 32-33 0.3463939146756232 1105 34-35 0.2670891999809939 1105 36-37 0.39337733870414127 1105 38-39 0.46159063110324894 1105 40-41 0.2927170477742109 1105 42-43 0.3167398599170852 1105 44-45 0.3430323488786513 1105 46-47 0.12863894629982298 1105 48-49 0.2367156927284242 1105 50-51 0.15738291495179624 1105 52-53 0.4655312813492287 1105 54-55 0.46464717009414613 1105 56-57 0.479884763545499 1105 58-59 0.44979605988530835 1105 60-61 0.49126269987929305 1105 62-63 0.17688652314146225 1105 64-65 0.3247347863892571 1105 66-67 0.27531846651283587 1105 68-69 0.20555129671703298 1105 70-71 0.21765380651353183 1105 72-73 0.23946016330922504 1105 74-75 0.2249828546607162 1105 76-77 0.08637613204473737 1105 78-79 0.27065590901803205 1105 80-81 0.46191769233906754 1105 82-83 0.4938718399365669 1105 84-85 0.6972054034699262 1105 86-87 0.6816749536867235 1105 88-89 0.2682918018282514 1105 90-91 0.728182503811805 1105 92-93 0.6277505264013996 1105 94-95 0.6333443090048085 1105 96-97 1.1642056155407694 1105 98-99 0.9517933836739729 1105 100-101 1.1391050900007826 1106 1 0.24666874569077635 1106 2 0.192816824985087 1106 3 0.1388691642465858 1106 4 0.0960879184317136 1106 5 0.05355308327114727 1106 6 0.16137150918262932 1106 7 0.09238024643853038 1106 8 0.08676490393016678 1106 9 0.3464362350365491 1106 10-11 0.21863078094043686 1106 12-13 0.31158648788930066 1106 14-15 0.4166110456320098 1106 16-17 0.44434028863665986 1106 18-19 0.33674207591251104 1106 20-21 0.2669107814155893 1106 22-23 0.3386426537105436 1106 24-25 0.43338713263725026 1106 26-27 0.37384020443175103 1106 28-29 0.4697541647181396 1106 30-31 0.46592438458822727 1106 32-33 0.4830737763554893 1106 34-35 0.41726892516071956 1106 36-37 0.38205465238145564 1106 38-39 0.47438069389330906 1106 40-41 0.4523130073701651 1106 42-43 0.6699316881089104 1106 44-45 0.6910280718743707 1106 46-47 0.29131753397841464 1106 48-49 0.3219759529886872 1106 50-51 0.245684853253735 1106 52-53 0.423709914527862 1106 54-55 0.6796280600750322 1106 56-57 0.6878245214852612 1106 58-59 0.6127681228573678 1106 60-61 0.5323264909430918 1106 62-63 0.6665853128402546 1106 64-65 0.48902180067626944 1106 66-67 0.635567351761722 1106 68-69 0.6521933933591271 1106 70-71 0.5720104358701619 1106 72-73 0.46467856352762027 1106 74-75 0.6154505951284506 1106 76-77 0.5639218595904687 1106 78-79 0.6374113007734223 1106 80-81 0.732677088098459 1106 82-83 0.6867783828431087 1106 84-85 0.6637969950615172 1106 86-87 0.6288084508202161 1106 88-89 0.8324218409582933 1106 90-91 0.9286397792690835 1106 92-93 0.8528779265288016 1106 94-95 1.0717622724227702 1106 96-97 1.3265224778576332 1106 98-99 1.207355764236354 1106 100-101 1.4986737495694413 1107 1 0.128669430075405 1107 2 0.05621175293459402 1107 3 0.11892505565755584 1107 4 0.1279726376439143 1107 5 0.08735788156779023 1107 6 0.1502635665188521 1107 7 0.16588505614158322 1107 8 0.049737794694777904 1107 9 0.029469199562612403 1107 10-11 0.18471096800556097 1107 12-13 0.19457192570535398 1107 14-15 -0.10170289680363709 1107 16-17 0.057321734209075714 1107 18-19 0.2025352016491766 1107 20-21 0.053632769172708095 1107 22-23 0.1016079402015535 1107 24-25 0.17149499925829303 1107 26-27 0.24308186824681854 1107 28-29 0.06393309327599184 1107 30-31 0.02888206680562888 1107 32-33 -0.20822218197309184 1107 34-35 -0.10396315726960381 1107 36-37 -0.13852156145071604 1107 38-39 0.125783682372834 1107 40-41 0.11064539016930297 1107 42-43 0.03377621873512027 1107 44-45 0.27202784374617295 1107 46-47 -0.1552082635197749 1107 48-49 -0.3675158914278782 1107 50-51 -0.2908783952919478 1107 52-53 -0.1745686680355405 1107 54-55 -0.21325909250471398 1107 56-57 -0.11815053649732477 1107 58-59 -0.2802815710706241 1107 60-61 -0.22204596637792662 1107 62-63 -0.2375894333671198 1107 64-65 -0.28162000001572096 1107 66-67 -0.31236143421599394 1107 68-69 -0.3585799612661731 1107 70-71 -0.3318040578753241 1107 72-73 -0.2640703433580427 1107 74-75 -0.12197954060782834 1107 76-77 -0.1640637683324293 1107 78-79 -0.17668226981324509 1107 80-81 -0.26999866567515696 1107 82-83 -0.11431853362394406 1107 84-85 -0.04579104341610574 1107 86-87 0.1985795621848041 1107 88-89 0.014942467550437044 1107 90-91 -0.17082126783939344 1107 92-93 -0.06289808959226306 1107 94-95 0.06284721215890343 1107 96-97 0.3619089650634386 1107 98-99 0.32560697141478556 1107 100-101 -0.2310969815814623 1108 1 -0.12297900794769134 1108 2 -0.18959546496780888 1108 3 -0.21117302094641133 1108 4 -0.054527282299495994 1108 5 0.05424210809010077 1108 6 0.044732965977964056 1108 7 -0.011834757428445641 1108 8 -0.011319185221211114 1108 9 -0.10010457913767823 1108 10-11 0.010420487753343366 1108 12-13 0.0017854613829371146 1108 14-15 -0.1704504041324597 1108 16-17 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-0.35195969544132666 2316 82-83 -0.27293538299074527 2316 84-85 -0.3451521966243938 2316 86-87 -0.3036255893505455 2316 88-89 -0.4061690331406673 2316 90-91 -0.22723711904430743 2316 92-93 -0.4275773187647829 2316 94-95 -0.546634746262928 2316 96-97 -0.44173982181344584 2316 98-99 -0.41430250436187066 2316 100-101 -0.3807299875710122 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 6.0 5 21.0 6 42.0 7 114.0 8 224.0 9 489.0 10 936.0 11 1530.0 12 2421.0 13 3371.0 14 4386.0 15 6221.0 16 8469.0 17 12265.0 18 11176.0 19 6130.0 20 4963.0 21 4891.0 22 4909.0 23 5315.0 24 6105.0 25 7276.0 26 8749.0 27 10857.0 28 13392.0 29 16674.0 30 21421.0 31 26443.0 32 33622.0 33 44382.0 34 60156.0 35 86641.0 36 131505.0 37 209439.0 38 179136.0 39 17085.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.317613271757544 20.228805268307053 15.969134958289063 21.484446501646346 2 15.09639363988148 21.435581028191812 40.88848966643772 22.579535665488983 3 18.68493185265279 26.141802018230127 31.333264614497853 23.84000151461923 4 12.39942277878165 18.30331881164792 38.65520498295052 30.64205342661991 5 12.811030438043188 37.97471682911719 34.6495559825864 14.56469675025322 6 29.42944711715445 38.02287007684363 18.16879513484973 14.37888767115219 7 26.390909196087776 34.38501498270337 21.75631571695575 17.46776010425311 8 25.7942305285334 34.63207970861298 20.467876852591026 19.1058129102626 9 26.127962489074562 17.01948855813699 21.354112433908345 35.4984365188801 10-11 23.6904313016461 27.72351425673 28.02511460550306 20.560939836120838 12-13 25.466273926366977 25.25817626087885 28.73425396960628 20.541295843147896 14-15 22.44350064106407 26.14095205640102 28.05026068536533 23.365286617169577 16-17 20.638108550711376 29.462179323343463 28.410918388704655 21.48879373724051 18-19 20.34061064639663 27.168390019384432 32.25330602894728 20.237693305271662 20-21 21.26086990820236 26.268955778833693 32.46822844490958 20.001945868054367 22-23 20.96663416645368 26.3535707636919 32.38025669909326 20.299538370761166 24-25 20.87264343200762 26.342074247899355 32.52906611354231 20.256216206550718 26-27 20.759344952697397 26.591430574378606 32.40305845811765 20.246166014806356 28-29 20.45521001844906 26.72320942101089 32.514152342525605 20.307428218014444 30-31 20.780403903860467 26.46467090435226 32.40183147967463 20.353093712112642 32-33 20.604414386461286 26.496941904065718 32.38310605781721 20.515537651655787 34-35 20.844964648515155 26.551216002726303 32.30111765807194 20.302701690686604 36-37 20.912606919416525 26.34251448382062 32.19841677220116 20.5464618245617 38-39 20.68343857630374 26.70739999989482 32.30947796787017 20.29968345593127 40-41 20.724066189278112 26.690073291204214 32.17297691874016 20.41288360077751 42-43 20.748579800935474 26.589355527811286 31.903229776165475 20.75883489508776 44-45 20.96029795205294 26.79265048797958 31.45677602163329 20.790275538334193 46-47 21.04486771919141 27.20734905651943 30.984268925487623 20.76351429880154 48-49 21.18638586017629 27.474661100601992 30.46011013277252 20.8788429064492 50-51 21.08220621049548 27.65777747626508 30.238971603288523 21.021044709950917 52-53 21.374621482641583 27.792508853545993 29.857407160039084 20.97546250377334 54-55 21.1349648242316 27.65919290057961 30.03253830890199 21.1733039662868 56-57 21.289937828619028 27.896133510174504 29.77836654714812 21.035562114058347 58-59 21.353788126599397 27.636321755419686 29.95018653753312 21.059703580447795 60-61 21.309315765420614 27.479033624560017 30.339253756382472 20.8723968536369 62-63 21.151853826873783 27.649004010463173 30.303713964468535 20.8954281981945 64-65 21.09619499737842 27.71410074976742 30.054277544894276 21.135426707959883 66-67 21.046118797343606 27.9056167579268 29.797467715369358 21.25079672936024 68-69 21.167157325221954 27.632438367921342 29.989124524197937 21.21127978265877 70-71 21.15456925843127 27.72677480104106 29.987751923063776 21.130904017463898 72-73 21.206831993706103 27.6154284668508 29.830756803490253 21.346982735952846 74-75 21.170510009660727 27.83709603878596 29.64328931750664 21.34910463404668 76-77 21.047984319887753 27.86143905103136 29.65437595351501 21.43620067556588 78-79 21.1076594918915 27.912047343103396 29.54379200251588 21.436501162489225 80-81 21.16086402943418 28.00143889205312 29.435229610042374 21.40246746847033 82-83 21.1475394317785 28.004451194628277 29.51414453340289 21.333864840190333 84-85 20.978636663402547 27.96282057889583 29.451764465821924 21.606778291879703 86-87 20.958166338593546 27.895032729303654 29.373544246747446 21.773256685355353 88-89 21.046207341931385 27.641418741677693 29.386762982798984 21.925610933591937 90-91 20.836381325515045 27.632505489015397 29.69637527773761 21.834737907731952 92-93 20.921565100871618 27.517162531566754 29.696780375340648 21.864491992220987 94-95 20.698860125425643 27.548415087889982 29.65051734791812 22.102207438766253 96-97 20.79424163507777 27.465353886801662 29.657714492467047 22.082689985653523 98-99 20.673934296822864 27.50594139020379 29.432114062070237 22.388010250903108 100-101 20.27863060697949 27.731796873438746 29.473040955785756 22.516531563796008 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 713.0 1 500.0 2 313.5 3 358.5 4 299.0 5 290.5 6 598.0 7 1289.5 8 2185.5 9 2638.0 10 2267.5 11 1771.5 12 1679.5 13 1831.5 14 1990.5 15 2037.5 16 2132.0 17 2271.5 18 2395.5 19 2502.5 20 2719.0 21 3096.5 22 3544.0 23 4124.0 24 4854.5 25 5878.0 26 7212.5 27 8798.0 28 10085.5 29 11576.0 30 13860.5 31 15945.0 32 18036.5 33 20303.5 34 23183.5 35 25717.5 36 28339.5 37 31016.5 38 32250.0 39 32511.5 40 32674.5 41 33420.0 42 33669.5 43 34159.0 44 35073.5 45 35507.5 46 35097.5 47 34807.0 48 36162.0 49 35613.0 50 32540.0 51 29847.0 52 27388.5 53 25881.5 54 24262.5 55 21811.0 56 19301.0 57 16919.5 58 15215.0 59 13479.5 60 11214.0 61 8750.0 62 6778.0 63 5180.0 64 3712.5 65 2695.5 66 1951.0 67 1532.5 68 1118.5 69 684.0 70 488.0 71 326.5 72 213.5 73 162.0 74 125.0 75 94.5 76 62.5 77 32.5 78 15.5 79 12.0 80 9.0 81 3.5 82 2.0 83 1.5 84 1.0 85 1.0 86 2.5 87 2.0 88 0.0 89 0.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01819591212531409 2 0.0027346457529373778 3 0.003050181801353229 4 1.0517868280528376E-4 5 0.001262144193663405 6 1.0517868280528376E-4 7 0.0 8 0.0 9 0.0 10-11 1.5776802420792562E-4 12-13 7.888401210396283E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0032079498255611545 22-23 0.0 24-25 0.004470094019224559 26-27 0.0023665203631188843 28-29 0.003891611263795499 30-31 0.017407072004274462 32-33 8.414294624422701E-4 34-35 0.0026294670701320936 36-37 0.004943398091848337 38-39 0.0027872350943400196 40-41 0.0036812538981849316 42-43 0.0016828589248845402 44-45 6.310720968317025E-4 46-47 1.5776802420792562E-4 48-49 1.5776802420792562E-4 50-51 1.5776802420792562E-4 52-53 0.0014725015592739726 54-55 0.003944200605198141 56-57 4.2071473122113506E-4 58-59 0.0016828589248845402 60-61 0.0015250909006766144 62-63 0.0 64-65 1.5776802420792562E-4 66-67 1.0517868280528376E-4 68-69 0.0 70-71 1.5776802420792562E-4 72-73 1.0517868280528376E-4 74-75 6.836614382343444E-4 76-77 4.733040726237769E-4 78-79 1.5776802420792562E-4 80-81 0.003313128508366438 82-83 7.362507796369863E-4 84-85 0.0023139310217162425 86-87 9.991974866501957E-4 88-89 5.258934140264188E-4 90-91 5.258934140264188E-5 92-93 0.0 94-95 5.258934140264188E-5 96-97 7.362507796369863E-4 98-99 0.0018406269490924658 100-101 6.310720968317025E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 950763.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.78060666355574 #Duplication Level Percentage of deduplicated Percentage of total 1 82.58562468318722 52.67361243982405 2 10.64338600750297 13.576832330258798 3 2.961000708576293 5.665632645726433 4 1.2177595966581864 3.1067778338090433 5 0.6714496312349741 2.1412732412093716 6 0.39283146448424056 1.5033017472802745 7 0.2651019446109588 1.1835853998482713 8 0.19720770059616674 1.0062421426198702 9 0.1421108952263061 0.8157527199931329 >10 0.8267293053147646 9.817091943302195 >50 0.06764696473068427 2.8952069032203744 >100 0.02765189865380096 3.122094017470419 >500 6.663107659598559E-4 0.2933404079299074 >1k 4.997330744698919E-4 0.7233109370470154 >5k 3.3315538297992795E-4 1.4759452904608017 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7889 0.8297546286508836 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6003 0.6313876328801183 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4156 0.4371226057387593 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1526 0.16050266996086301 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1126 0.11843119683874952 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.0517868280528375E-4 0.0 0.0 0.0 0.0 7 1.0517868280528375E-4 0.0 0.0 0.0 0.0 8 1.0517868280528375E-4 0.0 0.0 1.0517868280528375E-4 0.0 9 1.0517868280528375E-4 0.0 0.0 1.0517868280528375E-4 0.0 10-11 1.0517868280528375E-4 0.0 0.0 2.103573656105675E-4 0.0 12-13 2.103573656105675E-4 0.0 0.0 2.103573656105675E-4 0.0 14-15 4.20714731221135E-4 0.0 0.0 2.103573656105675E-4 0.0 16-17 4.20714731221135E-4 0.0 0.0 2.103573656105675E-4 0.0 18-19 5.784827554290606E-4 0.0 0.0 2.103573656105675E-4 0.0 20-21 6.310720968317026E-4 0.0 0.0 2.629467070132094E-4 0.0 22-23 6.310720968317026E-4 0.0 0.0 5.258934140264188E-4 0.0 24-25 6.310720968317026E-4 0.0 0.0 8.414294624422701E-4 0.0 26-27 6.310720968317026E-4 0.0 0.0 0.0011569655108581212 0.0 28-29 7.888401210396282E-4 0.0 0.0 0.0023139310217162425 0.0 30-31 8.4142946244227E-4 0.0 0.0 0.004627862043432485 0.0 32-33 8.4142946244227E-4 0.0 0.0 0.007730633186188355 0.0 34-35 8.4142946244227E-4 0.0 0.0 0.010254921573515166 0.0 36-37 8.4142946244227E-4 0.0 0.0 0.013410282057673679 0.0 38-39 9.466081452475538E-4 0.0 0.0 0.017827786735495597 0.0 40-41 9.466081452475538E-4 0.0 0.0 0.020509843147030332 0.0 42-43 9.991974866501957E-4 0.0 0.0 0.02966038855109002 0.0 44-45 0.0010517868280528375 0.0 0.0 0.03996789946600783 0.0 46-47 0.0011569655108581212 0.0 0.0 0.04906585552866487 0.0 48-49 0.0012621441936634052 0.0 0.0 0.06289685231755969 0.0 50-51 0.0012621441936634052 0.0 0.0 0.07825294000713112 0.0 52-53 0.001314733535066047 0.0 0.0 0.10938583011749511 0.0 54-55 0.0013673228764686889 0.0 0.0 0.13568050081881605 0.0 56-57 0.0014725015592739726 0.0 0.0 0.18721805539340508 0.0 58-59 0.0014725015592739726 0.0 0.0 0.24133248769672358 0.0 60-61 0.0014725015592739726 0.0 0.0 0.29828674443578473 0.0 62-63 0.0014725015592739726 0.0 0.0 0.38342888816666193 0.0 64-65 0.0014725015592739726 0.0 0.0 0.42350196631547504 0.0 66-67 0.0014725015592739726 0.0 0.0 0.5319937776291253 0.0 68-69 0.0015776802420792563 0.0 0.0 0.6713555323461262 0.0 70-71 0.0015776802420792563 0.0 0.0 0.763018754410931 0.0 72-73 0.0015776802420792563 0.0 0.0 0.9253094619794838 0.0 74-75 0.0016302695834818982 0.0 0.0 1.0879157055964526 0.0 76-77 0.00168285892488454 0.0 0.0 1.1786849088574125 0.0 78-79 0.00168285892488454 0.0 0.0 1.2763433158421185 0.0 80-81 0.00168285892488454 0.0 0.0 1.474342186223065 0.0 82-83 0.00168285892488454 0.0 0.0 1.6251684173658418 1.0517868280528375E-4 84-85 0.001788037607689824 0.0 0.0 1.8828036008973845 1.0517868280528375E-4 86-87 0.0019458056318977495 0.0 0.0 2.2717017805699213 1.0517868280528375E-4 88-89 0.0019983949733003914 0.0 0.0 2.77529731384162 1.0517868280528375E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3630 0.0 42.92539 1 GTATCAA 6055 0.0 37.34563 1 TAGACCG 60 0.003950269 31.667227 5 ATCAACG 7600 0.0 29.688026 3 TATCAAC 7630 0.0 29.5713 2 TCAACGC 7670 0.0 29.417078 4 CAACGCA 7785 0.0 28.921515 5 AACGCAG 7985 0.0 28.13763 6 CGATCGT 35 0.008387898 27.146193 24-25 ACGCAGA 8960 0.0 24.968437 7 CGCAGAG 9050 0.0 24.667646 8 GAGTACT 5625 0.0 23.55918 12-13 GCAGAGT 9510 0.0 23.124853 9 GTACATG 4165 0.0 23.040047 1 GTGTACG 105 0.0021943764 22.621828 1 AGAGTAC 8030 0.0 21.885912 10-11 AGTACTT 5870 0.0 21.84762 12-13 GTACTTT 6115 0.0 21.55485 14-15 TACATGG 4325 0.0 21.526394 2 TAGGACA 355 0.0 21.40883 4 >>END_MODULE