FastQCFastQC Report
Thu 26 May 2016
SRR936830_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936830_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730667
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45690.6253190577924006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32560.4456202346623017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20710.28343965171548735No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7900.10812038863120957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42900.038.3363341
GGTATCA25300.034.0055541
TCAACGC52750.030.997584
ATCAACG52600.030.9934843
CAACGCA53550.030.5449665
TATCAAC54200.030.0806122
AACGCAG55100.029.675546
ACGCAGA62700.025.854627
CGCAGAG62700.025.7805678
GTACATG33500.024.404811
GCAGAGT65700.024.2415589
ACCGTGC1401.7660876E-523.77128
TCCGTCG500.001651209723.75654280-81
AGTACTT35650.022.80200412-13
GAGTACT34100.022.44439912-13
AGAGTAC54700.022.16327710-11
TACATGG36300.022.1295152
ACATGGG35350.021.7814923
GTACTTT37900.020.18919214-15
CATGGGG13900.019.8337574
Produced by FastQC (version 0.11.5)