##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936830_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 730667 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.906878235913215 33.0 31.0 34.0 30.0 34.0 2 32.02228648618317 33.0 31.0 34.0 30.0 34.0 3 32.027458472874784 34.0 31.0 34.0 30.0 34.0 4 35.61294816927547 37.0 35.0 37.0 33.0 37.0 5 35.61588794895623 37.0 35.0 37.0 33.0 37.0 6 35.696585448638025 37.0 35.0 37.0 33.0 37.0 7 35.63738611433115 37.0 35.0 37.0 33.0 37.0 8 35.58844863665664 37.0 35.0 37.0 33.0 37.0 9 37.25137579773002 39.0 37.0 39.0 34.0 39.0 10-11 37.07658002893247 39.0 37.0 39.0 33.0 39.0 12-13 37.027240863485005 39.0 37.0 39.0 33.0 39.0 14-15 38.20571819994608 40.0 38.0 41.0 33.0 41.0 16-17 38.153753351389895 40.0 38.0 41.0 33.0 41.0 18-19 38.1610179466159 40.0 38.0 41.0 33.5 41.0 20-21 38.10872052521874 40.0 38.0 41.0 33.0 41.0 22-23 38.0040476715111 40.0 38.0 41.0 33.0 41.0 24-25 37.85961936148752 40.0 38.0 41.0 33.0 41.0 26-27 37.94115582064059 40.0 38.0 41.0 33.0 41.0 28-29 37.8907676137009 40.0 38.0 41.0 33.0 41.0 30-31 37.740654771599104 40.0 37.5 41.0 33.0 41.0 32-33 37.531901673402515 40.0 37.0 41.0 32.5 41.0 34-35 37.373241161842536 40.0 37.0 41.0 32.0 41.0 36-37 37.20377613878826 40.0 36.0 41.0 31.5 41.0 38-39 37.054651434921794 40.0 36.0 41.0 31.0 41.0 40-41 36.889401054105356 39.5 36.0 41.0 31.0 41.0 42-43 36.670948599019795 39.0 36.0 41.0 30.5 41.0 44-45 36.39397221990318 39.0 35.5 41.0 30.0 41.0 46-47 36.021181331577864 39.0 35.0 41.0 29.5 41.0 48-49 35.67441118868103 39.0 35.0 40.5 27.5 41.0 50-51 35.01273220222071 38.5 34.5 40.0 26.5 40.5 52-53 35.24326608427642 38.5 35.0 40.0 27.5 40.5 54-55 35.62835464034916 39.0 35.0 41.0 28.0 41.0 56-57 35.53255244865308 39.0 35.0 41.0 27.0 41.0 58-59 35.31258836104546 39.0 35.0 41.0 26.5 41.0 60-61 35.06199404106111 39.0 35.0 40.5 26.0 41.0 62-63 34.77242026805645 38.0 34.5 40.0 26.0 41.0 64-65 34.470394174090245 38.0 34.0 40.0 25.5 41.0 66-67 34.11149401847901 37.0 34.0 40.0 24.5 41.0 68-69 33.745637889763735 37.0 34.0 39.5 23.0 41.0 70-71 33.34439149434695 36.0 33.5 39.0 22.5 41.0 72-73 32.953456909919296 36.0 33.0 39.0 22.0 40.5 74-75 32.52636632008836 35.0 33.0 38.5 20.5 40.0 76-77 32.08801889232715 35.0 32.0 37.0 19.5 39.5 78-79 31.63647119686533 35.0 32.0 37.0 18.0 39.0 80-81 31.206778874644677 35.0 31.5 36.0 15.0 39.0 82-83 30.80125282789561 35.0 31.0 36.0 10.0 37.5 84-85 30.430252084739013 35.0 31.0 35.5 7.0 37.0 86-87 30.098327281785 34.0 31.0 35.0 4.5 37.0 88-89 29.758406360216078 34.0 30.5 35.0 2.0 36.0 90-91 29.41255524062261 34.0 30.0 35.0 2.0 36.0 92-93 29.05755015622712 34.0 30.0 35.0 2.0 35.5 94-95 28.6638995602648 34.0 29.0 35.0 2.0 35.0 96-97 28.219159343449206 34.0 29.0 35.0 2.0 35.0 98-99 27.659921687991933 34.0 29.0 35.0 2.0 35.0 100-101 25.527463263018586 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5833382923659052 1101 2 0.557761506203633 1101 3 0.5876981887360628 1101 4 0.48799282295921387 1101 5 0.4936046712256754 1101 6 0.3613873567175503 1101 7 0.3859136384418207 1101 8 0.3962793665196571 1101 9 0.37041119942295353 1101 10-11 0.5167996028051149 1101 12-13 0.4784228096765446 1101 14-15 0.7082909339285735 1101 16-17 0.8047701926228612 1101 18-19 0.6848253250297418 1101 20-21 0.6158372403511336 1101 22-23 0.8105798985607322 1101 24-25 0.5976578665687029 1101 26-27 0.7110738034351272 1101 28-29 0.8563290867999953 1101 30-31 0.8551086796348457 1101 32-33 0.8300437498836075 1101 34-35 0.7647552544540659 1101 36-37 0.8111457624755971 1101 38-39 0.9806135156851568 1101 40-41 1.0221761485430747 1101 42-43 0.9791965285072877 1101 44-45 1.1395593258764123 1101 46-47 0.9743179781575577 1101 48-49 1.2352645180926487 1101 50-51 1.2673488532468369 1101 52-53 1.3052788759957465 1101 54-55 1.32597641622597 1101 56-57 1.3598337844653443 1101 58-59 1.3784737082148553 1101 60-61 1.4584608732647766 1101 62-63 1.3397518301282432 1101 64-65 1.3577645090567003 1101 66-67 1.3800754156320423 1101 68-69 1.2647034722224006 1101 70-71 1.2905597852779138 1101 72-73 1.2624344171682225 1101 74-75 1.2465560273454415 1101 76-77 1.2487496925065678 1101 78-79 1.4241656294417773 1101 80-81 1.229291760852842 1101 82-83 1.4228401857639845 1101 84-85 1.338222467479941 1101 86-87 1.2106858751662486 1101 88-89 1.363571932351956 1101 90-91 1.3787049444430153 1101 92-93 1.3436560545516478 1101 94-95 1.4329014939266713 1101 96-97 1.5214993473404839 1101 98-99 1.619545432443413 1101 100-101 1.6433873806191954 1102 1 0.36352806641191293 1102 2 0.2744187865640697 1102 3 0.323047018007685 1102 4 0.3152890547839178 1102 5 0.10214876979895138 1102 6 0.2111808579804375 1102 7 0.34173400160653955 1102 8 0.3597069042355443 1102 9 0.5459479254793393 1102 10-11 0.528359692231021 1102 12-13 0.39500201303513194 1102 14-15 0.3652555731530924 1102 16-17 0.5635101236761741 1102 18-19 0.4348999888293079 1102 20-21 0.42643864659047637 1102 22-23 0.54961285302862 1102 24-25 0.40235443288722905 1102 26-27 0.3590830532132756 1102 28-29 0.29211608591256777 1102 30-31 0.398185425487803 1102 32-33 0.415580731930163 1102 34-35 0.22735082411752927 1102 36-37 0.11963311656119657 1102 38-39 0.33724195448644423 1102 40-41 0.3701371868391945 1102 42-43 0.35670096567022824 1102 44-45 0.470507684126126 1102 46-47 0.6011632406328076 1102 48-49 0.664446970819796 1102 50-51 0.7118565841499702 1102 52-53 0.5825012973214356 1102 54-55 0.7130570189974961 1102 56-57 0.7758348766900696 1102 58-59 0.7964160592285765 1102 60-61 0.7687124262718115 1102 62-63 0.9110152269471428 1102 64-65 1.0564086144563873 1102 66-67 0.5323554347459805 1102 68-69 0.6408063632047174 1102 70-71 0.5027201716524132 1102 72-73 0.5832028996584953 1102 74-75 0.46948506687031966 1102 76-77 0.4769350635216547 1102 78-79 0.5388577585973557 1102 80-81 0.5577463481966909 1102 82-83 0.5095675221009373 1102 84-85 0.701421320643977 1102 86-87 0.45158969112638303 1102 88-89 0.35651513666624624 1102 90-91 0.5165577842273024 1102 92-93 0.5284574915098013 1102 94-95 0.46180065086571176 1102 96-97 0.7044619215776322 1102 98-99 0.7347175431676973 1102 100-101 0.9218726867834377 1103 1 0.26373312451170605 1103 2 0.2975875038117337 1103 3 0.06532885897373575 1103 4 0.17241767178095557 1103 5 0.18142366157384515 1103 6 0.08344674998316748 1103 7 0.08118205355459196 1103 8 0.288904899223013 1103 9 0.12986510479125002 1103 10-11 0.3301155022105178 1103 12-13 0.18089430057216305 1103 14-15 0.28662148794006725 1103 16-17 0.33457841941552857 1103 18-19 0.4534762590503192 1103 20-21 0.49322493048202176 1103 22-23 0.6554242565373869 1103 24-25 0.5691635010120777 1103 26-27 0.5477507451704398 1103 28-29 0.4784697541741352 1103 30-31 0.5222954163741065 1103 32-33 0.5062548604332449 1103 34-35 0.5876977140696837 1103 36-37 0.5921709953490719 1103 38-39 0.6389906443930258 1103 40-41 0.5970325502477252 1103 42-43 0.6892795712751507 1103 44-45 0.7605082537319632 1103 46-47 0.8219353413391204 1103 48-49 0.9679400785892227 1103 50-51 0.9119662332727714 1103 52-53 0.9141756536668453 1103 54-55 0.7981347701936699 1103 56-57 0.8820094039172304 1103 58-59 1.0879545645828728 1103 60-61 0.8822063884499798 1103 62-63 0.9756227685283676 1103 64-65 0.9822886554680039 1103 66-67 0.9162697660287336 1103 68-69 0.8324431255651632 1103 70-71 0.6257123110919238 1103 72-73 0.5223262762818734 1103 74-75 0.6553332099553089 1103 76-77 0.7121110717239851 1103 78-79 0.6509561864852635 1103 80-81 0.7595505746719695 1103 82-83 0.8799038743451852 1103 84-85 0.8099597120771058 1103 86-87 0.9338464694357889 1103 88-89 1.0248336602479249 1103 90-91 1.0718593905157476 1103 92-93 0.9770719253216065 1103 94-95 0.9833103911254533 1103 96-97 1.0481976814318124 1103 98-99 1.0902299036142686 1103 100-101 1.0925194742065436 1104 1 0.48728855458887566 1104 2 0.4036481098723428 1104 3 0.48682330655513795 1104 4 0.521749499373648 1104 5 0.43136719629998765 1104 6 0.35940195419924237 1104 7 0.37250522313863144 1104 8 0.4563997232395778 1104 9 0.4283781859130329 1104 10-11 0.6333293173808485 1104 12-13 0.4982526668435696 1104 14-15 0.5445925024328133 1104 16-17 0.7474666181732843 1104 18-19 0.5894963983313275 1104 20-21 0.5426885103803798 1104 22-23 0.48321928759328614 1104 24-25 0.7459280408201039 1104 26-27 0.4848907790496 1104 28-29 0.5674354985746604 1104 30-31 0.7612799825325922 1104 32-33 0.7503871773916444 1104 34-35 0.8649516195844527 1104 36-37 0.8855880855052973 1104 38-39 0.8395411808131286 1104 40-41 0.30981722933675115 1104 42-43 0.5129761645369655 1104 44-45 0.7873527860112759 1104 46-47 0.7844141668614242 1104 48-49 1.1754970144070285 1104 50-51 1.0593782415543487 1104 52-53 1.3024826365390467 1104 54-55 1.3152343372932336 1104 56-57 1.1862113613800531 1104 58-59 1.2200880108467516 1104 60-61 1.0481719446329194 1104 62-63 0.9584149763205758 1104 64-65 1.1004657682103343 1104 66-67 0.9768852375137698 1104 68-69 1.0738039386651081 1104 70-71 1.1014275378941036 1104 72-73 1.1227215331988702 1104 74-75 1.1720798690932739 1104 76-77 1.0487382126989466 1104 78-79 1.1204884658001468 1104 80-81 1.1926862795468018 1104 82-83 1.171739936833422 1104 84-85 1.1906579992971338 1104 86-87 1.1461276664995985 1104 88-89 1.153118057010584 1104 90-91 1.0634978245454896 1104 92-93 1.2425763484347243 1104 94-95 0.9756075866835161 1104 96-97 1.1040512481549456 1104 98-99 1.2095833762286112 1104 100-101 1.1181970574928215 1105 1 0.14340968101753404 1105 2 0.18239375447164718 1105 3 0.294466929575222 1105 4 0.1739486611168175 1105 5 0.14940486735992664 1105 6 0.10126429828852679 1105 7 0.0929196384629094 1105 8 0.12746471339258392 1105 9 0.2453756105456577 1105 10-11 0.25949202000166594 1105 12-13 0.2725292915973938 1105 14-15 0.2887507949474184 1105 16-17 0.14579599587700187 1105 18-19 0.18776602077910098 1105 20-21 0.3399852162666903 1105 22-23 0.3190421427162491 1105 24-25 0.28323804435491695 1105 26-27 0.1856145751074365 1105 28-29 0.18674149598245293 1105 30-31 -0.10225584537227661 1105 32-33 0.1603612553932976 1105 34-35 -0.009000980189988184 1105 36-37 0.06534788620889032 1105 38-39 -0.052431415931472714 1105 40-41 0.1412156706503609 1105 42-43 0.19425088722208272 1105 44-45 0.3923537117969289 1105 46-47 0.5142328811976355 1105 48-49 0.5771154560572853 1105 50-51 0.44990661267657117 1105 52-53 0.32332375488811493 1105 54-55 0.4107240779049306 1105 56-57 0.5349455599021624 1105 58-59 0.4525367980675412 1105 60-61 0.5812569990859586 1105 62-63 0.6063243839860846 1105 64-65 0.5683452140855465 1105 66-67 0.710290108829561 1105 68-69 0.665468501273466 1105 70-71 0.45787100605549114 1105 72-73 0.653832980959308 1105 74-75 0.7726782632759708 1105 76-77 0.7128589688133395 1105 78-79 0.5596643482421975 1105 80-81 0.695926529340607 1105 82-83 0.6575804660461664 1105 84-85 0.6293381656019044 1105 86-87 0.7012152794875277 1105 88-89 0.6154831868730639 1105 90-91 0.6697679183023268 1105 92-93 0.5277309546391713 1105 94-95 0.668863658995793 1105 96-97 0.6026709859051174 1105 98-99 0.6227194330550176 1105 100-101 0.5345750473596773 1106 1 0.31963093040951307 1106 2 0.2856179918422228 1106 3 0.20381999746487622 1106 4 0.28356578292906676 1106 5 0.1692783994285847 1106 6 0.18121009774651498 1106 7 0.3141342865068282 1106 8 0.217069327387442 1106 9 0.18492115984730617 1106 10-11 0.3938989409939566 1106 12-13 0.46283920751706376 1106 14-15 0.5996048009233732 1106 16-17 0.5661107759478838 1106 18-19 0.4699736505477077 1106 20-21 0.49562068287777095 1106 22-23 0.48270345881658727 1106 24-25 0.6127495445981239 1106 26-27 0.554230376650068 1106 28-29 0.42767332837770056 1106 30-31 0.6213213904000767 1106 32-33 0.5264060055843842 1106 34-35 0.5873092136811735 1106 36-37 0.6204343986124812 1106 38-39 0.6520192824216693 1106 40-41 0.6444989727141461 1106 42-43 0.5393276713232495 1106 44-45 0.5793976976214026 1106 46-47 0.6693471887509688 1106 48-49 0.6591285197776671 1106 50-51 0.2467240680306091 1106 52-53 0.25746662195781767 1106 54-55 0.3755110975699978 1106 56-57 0.3390939860018065 1106 58-59 0.39979550142381015 1106 60-61 0.6242005054441009 1106 62-63 0.42603809394369563 1106 64-65 0.39439969257904295 1106 66-67 0.2703971201560833 1106 68-69 0.063147606269645 1106 70-71 0.2754653358449488 1106 72-73 0.24826700222259745 1106 74-75 0.2651909448130425 1106 76-77 0.46155607116897457 1106 78-79 0.38945379998287066 1106 80-81 0.5208170859384822 1106 82-83 0.45993957938089025 1106 84-85 0.4050313071487004 1106 86-87 0.5675553531446731 1106 88-89 0.5980243584386251 1106 90-91 0.7583997020560602 1106 92-93 0.39797972122940095 1106 94-95 0.5354295682446271 1106 96-97 0.5107906440247767 1106 98-99 0.5842405226248886 1106 100-101 0.5944388511259184 1107 1 0.18165836406616265 1107 2 0.24473960264922567 1107 3 0.2413892218318523 1107 4 0.23600904295659575 1107 5 0.29152142897498123 1107 6 0.16170323132953968 1107 7 0.279702166400547 1107 8 0.27593004636052143 1107 9 0.2968965751935073 1107 10-11 0.33261136776818034 1107 12-13 0.2111426103148517 1107 14-15 0.3539703375922869 1107 16-17 0.35945803418277933 1107 18-19 0.4607784138243858 1107 20-21 0.34262482833697305 1107 22-23 0.14840178462727494 1107 24-25 0.5319991204488232 1107 26-27 0.34412642005734284 1107 28-29 0.4032867443001109 1107 30-31 0.5646734503251807 1107 32-33 0.5075073532587737 1107 34-35 0.6126381294595333 1107 36-37 0.6740572738645696 1107 38-39 0.33921317467173395 1107 40-41 0.8092449633028309 1107 42-43 0.6909090279607852 1107 44-45 0.8602425550112045 1107 46-47 0.6628809126217732 1107 48-49 0.645775819627211 1107 50-51 0.7738576190405624 1107 52-53 0.8335053876033243 1107 54-55 1.0124052843237337 1107 56-57 1.0327341473385943 1107 58-59 1.0084577505354986 1107 60-61 0.9728692368375462 1107 62-63 1.054803419338235 1107 64-65 0.9055856300290301 1107 66-67 0.8755941495104196 1107 68-69 0.7547490499778249 1107 70-71 0.811832136958941 1107 72-73 0.9961242479730998 1107 74-75 1.1121890881595036 1107 76-77 0.979682451654913 1107 78-79 1.1173000018459298 1107 80-81 1.104630588291311 1107 82-83 0.929258779739417 1107 84-85 1.122547872979876 1107 86-87 1.1291350291906426 1107 88-89 1.0897474449650808 1107 90-91 1.0868133036438223 1107 92-93 1.2866565354680155 1107 94-95 1.2954851077945513 1107 96-97 1.0482858423345824 1107 98-99 1.040114990437555 1107 100-101 1.2189786891489014 1108 1 0.18890456074509032 1108 2 0.18156635681713595 1108 3 0.16458948035825216 1108 4 0.10991527742014995 1108 5 0.16206764885500036 1108 6 0.03420911446234953 1108 7 0.23572367712276332 1108 8 0.17196431768066134 1108 9 0.2339719124378803 1108 10-11 0.28199363882316675 1108 12-13 0.32095004129161 1108 14-15 0.4004015132245087 1108 16-17 0.33621137348210794 1108 18-19 0.30553828257252036 1108 20-21 0.316032653732222 1108 22-23 0.281161719664226 1108 24-25 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2316 80-81 -3.2192743284950396 2316 82-83 -2.8781987686258823 2316 84-85 -3.1176576267244407 2316 86-87 -3.5139491559914156 2316 88-89 -3.3278520312535527 2316 90-91 -2.915224797226333 2316 92-93 -3.1110318568611213 2316 94-95 -3.7394054894851685 2316 96-97 -3.85451364233214 2316 98-99 -3.811726692421278 2316 100-101 -3.9082494936676895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1453.0 3 466.0 4 525.0 5 510.0 6 575.0 7 647.0 8 771.0 9 940.0 10 1224.0 11 1580.0 12 2265.0 13 2723.0 14 3400.0 15 4127.0 16 5405.0 17 7585.0 18 6921.0 19 4112.0 20 3724.0 21 3929.0 22 4075.0 23 4576.0 24 4984.0 25 5762.0 26 6631.0 27 7869.0 28 9464.0 29 11390.0 30 14052.0 31 17879.0 32 22487.0 33 30082.0 34 41685.0 35 61487.0 36 94516.0 37 150150.0 38 158004.0 39 32667.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.989012253035874 20.141920500419026 15.212634265776401 21.656432980768702 2 14.70533060018376 21.394015826524424 41.38845641406942 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30.803064563180595 19.940272792140824 22-23 21.28551996456646 27.580517852656456 30.727708738515418 20.40625344426166 24-25 20.971240329655814 27.838613479859198 30.7936338455031 20.396512344981893 26-27 20.856347351865008 27.91564515223816 30.739200008760726 20.488807487136103 28-29 20.574171849097826 28.04344344316939 30.871935201700722 20.510449506032064 30-31 20.840471059102764 27.79219961495634 30.819028794777754 20.548300531163147 32-33 20.627346956373557 28.02872431650403 30.679968053676394 20.66396067344602 34-35 21.02223302067257 28.07314961519114 30.496572822809974 20.408044541326316 36-37 21.12638078963271 27.69538417107454 30.52173847582587 20.656496563466877 38-39 20.756886306227017 28.158489238617378 30.53530769636127 20.54931675879433 40-41 20.918005950038566 28.032045618331104 30.504795139775258 20.545153291855073 42-43 20.874625911653215 28.100373729057726 30.138603453691747 20.886396905597312 44-45 20.96894943796389 28.328180366168613 29.895421455230775 20.807448740636723 46-47 21.079004006829745 28.570509596203266 29.543645701811116 20.806840695155877 48-49 21.18088321940611 28.71308200228151 29.248080322285507 20.857954456026874 50-51 21.013067981563164 28.943919573228943 29.079424173883883 20.96358827132401 52-53 21.279756914622418 29.122545279099928 28.707753479125248 20.889944327152403 54-55 21.072583228166035 28.928511789231052 28.86534297313649 21.133562009466424 56-57 21.13391335300198 29.214372257627303 28.679896226221363 20.971818163149354 58-59 21.35712164120983 28.777975945238254 28.80145034855725 21.06345206499467 60-61 21.28484889968856 28.79646805161025 29.13152400835073 20.787159040350456 62-63 21.110447973674052 28.88387125034564 29.12253228460277 20.88314849137754 64-65 20.997824953220753 28.958441412671664 28.96713338689962 21.07660024720796 66-67 20.97697569710153 29.224259365986345 28.639856315427064 21.158908621485057 68-69 20.916608881188722 29.094240869951527 28.855957060997504 21.133193187862243 70-71 21.10780430976302 29.052501023298348 28.81738197160501 21.02231269533362 72-73 20.91670853394261 29.038458116068412 28.740915257439255 21.303918092549722 74-75 20.94255136236201 29.268538089188716 28.599472139477832 21.18943840897144 76-77 20.9989252979389 29.13777406613867 28.65073552061443 21.212565115308003 78-79 20.96824654640202 29.17192376651836 28.49390336155452 21.365926325525102 80-81 20.894980869953333 29.292896793586486 28.475189126986685 21.336933209473496 82-83 20.949354697829918 29.453420094656185 28.448742047525233 21.148483159988665 84-85 21.00630738850844 29.32309366625941 28.195361348679143 21.475237596553008 86-87 20.994519008232782 29.244072184531518 28.300454456155144 21.46095435108056 88-89 21.064465611131038 28.96647218746663 28.298640105790845 21.670422095611485 90-91 20.930199131852472 28.93455492723686 28.706840734215717 21.428405206694947 92-93 21.027010856506408 28.979800559015267 28.52737196421371 21.46581662026461 94-95 20.77746860657337 28.922338703329125 28.544488139120194 21.755704550977313 96-97 20.859331676559385 28.970403992688286 28.52307502721354 21.647189303538784 98-99 20.809070042619396 29.209623875625866 28.125102713836515 21.856203367918223 100-101 20.63061366352433 29.278882865977472 28.14654508223386 21.94395838826434 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 483.0 1 321.0 2 195.0 3 235.5 4 223.0 5 250.0 6 424.5 7 766.5 8 1253.5 9 1537.0 10 1396.5 11 1219.0 12 1229.5 13 1323.5 14 1377.5 15 1432.5 16 1512.0 17 1534.0 18 1606.0 19 1777.5 20 1991.0 21 2245.0 22 2607.5 23 3125.0 24 3720.0 25 4596.5 26 5746.5 27 7049.0 28 8185.5 29 9395.5 30 11099.0 31 12793.0 32 14405.5 33 16244.5 34 18654.5 35 20640.0 36 22717.0 37 24685.0 38 25375.5 39 25484.5 40 25525.5 41 25997.5 42 26351.0 43 26763.0 44 27313.5 45 27332.5 46 26595.0 47 26468.0 48 27781.5 49 27254.5 50 24800.0 51 22635.5 52 20593.0 53 19228.5 54 18011.5 55 16259.0 56 14498.5 57 12764.0 58 11229.5 59 9786.5 60 8005.5 61 6280.0 62 4926.5 63 3766.0 64 2780.5 65 2052.0 66 1474.0 67 1112.5 68 811.5 69 516.5 70 349.0 71 233.5 72 163.5 73 118.0 74 82.5 75 58.5 76 47.0 77 25.5 78 13.0 79 9.0 80 4.5 81 4.0 82 3.5 83 4.5 84 5.0 85 3.5 86 3.0 87 3.0 88 1.0 89 0.5 90 1.0 91 1.5 92 1.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.054607639321332425 2 0.05118610803553466 3 0.029014585303565096 4 0.052417859298421854 5 0.07349449201893613 6 0.08033755459053167 7 0.08334850212203371 8 0.08635944965353574 9 0.0845802533849209 10-11 0.09251820596797174 12-13 0.08957568906218565 14-15 0.08649631090496766 16-17 0.06843062571595543 18-19 0.05885033811572167 20-21 0.07034668323600217 22-23 0.039073887283810546 24-25 0.02018703458620685 26-27 0.01833940769187605 28-29 0.02107663272051427 30-31 0.013754555768907039 32-33 0.00910127322022207 34-35 0.014986307031794238 36-37 0.008416966963062517 38-39 0.009374995723085892 40-41 0.011427914494564555 42-43 0.007869521957334874 44-45 0.002737225028638217 46-47 0.005406019431560479 48-49 0.0014370431400350639 50-51 0.00882755071735825 52-53 0.008143244460198696 54-55 0.011633206371712421 56-57 0.03175181033220331 58-59 0.011496345120280511 60-61 0.026277360274926883 62-63 0.01820254644044414 64-65 0.01478101515464637 66-67 0.02436130275488013 68-69 0.03223082471221501 70-71 0.025456192766335418 72-73 0.025182470263471597 74-75 0.023403273994856755 76-77 0.03175181033220331 78-79 0.041811112312448766 80-81 0.0371578297637638 82-83 0.032025532835067136 84-85 0.023882288374868443 86-87 0.03195710220935118 88-89 0.023129551491992934 90-91 0.019981742709058985 92-93 0.03770527476949144 94-95 0.03004104468930443 96-97 0.045437935475394396 98-99 0.06610398444161293 100-101 0.07999540146195189 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 730667.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.80369042756202 #Duplication Level Percentage of deduplicated Percentage of total 1 85.66020493821436 59.79398427468634 2 9.115816639643956 12.726352854162506 3 2.3020989542007455 4.820850081979293 4 0.9259097387918221 2.585276670819566 5 0.47910737620254784 1.672173148500207 6 0.29309284201064495 1.2275377206147238 7 0.19334763553909268 0.944746494725034 8 0.1529326168128805 0.8540208832226621 9 0.11908346666573873 0.7481218897958513 >10 0.6973197848486756 9.06113664462935 >50 0.04380154516826455 2.104597306469828 >100 0.01590956152538009 1.7365650603107612 >500 7.856573576321002E-4 0.3678512593000334 >1k 5.892430182240752E-4 1.3567857107838726 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4569 0.6253190577924006 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3256 0.4456202346623017 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2071 0.28343965171548735 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 790 0.10812038863120957 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.843062571595542E-5 0.0 18-19 1.3686125143191083E-4 0.0 0.0 1.3686125143191083E-4 0.0 20-21 1.3686125143191083E-4 0.0 0.0 1.3686125143191083E-4 0.0 22-23 1.3686125143191083E-4 0.0 0.0 2.7372250286382166E-4 0.0 24-25 2.7372250286382166E-4 0.0 0.0 4.1058375429573255E-4 0.0 26-27 2.7372250286382166E-4 0.0 0.0 8.21167508591465E-4 0.0 28-29 5.474450057276433E-4 0.0 0.0 0.002737225028638217 0.0 30-31 6.843062571595543E-4 0.0 0.0 0.0057481725601402555 0.0 32-33 6.843062571595543E-4 0.0 0.0 0.008964411968790161 0.0 34-35 6.843062571595543E-4 0.0 0.0 0.013343972014611308 0.0 36-37 6.843062571595543E-4 0.0 0.0 0.01662864204897717 0.0 38-39 6.843062571595543E-4 0.0 0.0 0.02155564710052596 0.0 40-41 6.843062571595543E-4 0.0 0.0 0.026208929649210927 0.0 42-43 6.843062571595543E-4 0.0 0.0 0.03941604041239033 0.0 44-45 6.843062571595543E-4 0.0 0.0 0.05337588805844523 0.0 46-47 8.211675085914651E-4 0.0 0.0 0.06295617565867899 0.0 48-49 9.580287600233759E-4 0.0 0.0 0.07650543955043816 0.0 50-51 9.580287600233759E-4 0.0 0.0 0.09402367973372275 0.0 52-53 9.580287600233759E-4 0.0 0.0 0.1359716532976034 0.0 54-55 9.580287600233759E-4 0.0 0.0 0.17005010490414924 0.0 56-57 9.580287600233759E-4 0.0 0.0 0.23499076870859092 0.0 58-59 9.580287600233759E-4 0.0 0.0 0.308622121978959 0.0 60-61 9.580287600233759E-4 0.0 0.0 0.388343800938047 0.0 62-63 0.0010948900114552866 0.0 0.0 0.5007068883636459 0.0 64-65 0.0010948900114552866 0.0 0.0 0.5521667189020443 0.0 66-67 0.0010948900114552866 0.0 0.0 0.6869750515624764 0.0 68-69 0.0010948900114552866 0.0 0.0 0.8735169372641709 0.0 70-71 0.0012317512628871976 0.0 0.0 0.99162819724991 0.0 72-73 0.001300181888603153 0.0 0.0 1.20362627571794 0.0 74-75 0.0013686125143191085 0.0 0.0 1.4176772729574485 0.0 76-77 0.0013686125143191085 0.0 0.0 1.5398943704861447 0.0 78-79 0.0013686125143191085 0.0 0.0 1.6523258885374597 0.0 80-81 0.0016423350171829302 0.0 0.0 1.8865639203631752 0.0 82-83 0.0016423350171829302 0.0 0.0 2.0674944947561613 0.0 84-85 0.0016423350171829302 0.0 0.0 2.3830281099324315 0.0 86-87 0.0016423350171829302 0.0 0.0 2.870254165030034 0.0 88-89 0.0016423350171829302 0.0 0.0 3.5027584385226103 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4290 0.0 38.336334 1 GGTATCA 2530 0.0 34.005554 1 TCAACGC 5275 0.0 30.99758 4 ATCAACG 5260 0.0 30.993484 3 CAACGCA 5355 0.0 30.544966 5 TATCAAC 5420 0.0 30.080612 2 AACGCAG 5510 0.0 29.67554 6 ACGCAGA 6270 0.0 25.85462 7 CGCAGAG 6270 0.0 25.780567 8 GTACATG 3350 0.0 24.40481 1 GCAGAGT 6570 0.0 24.241558 9 ACCGTGC 140 1.7660876E-5 23.7712 8 TCCGTCG 50 0.0016512097 23.756542 80-81 AGTACTT 3565 0.0 22.802004 12-13 GAGTACT 3410 0.0 22.444399 12-13 AGAGTAC 5470 0.0 22.163277 10-11 TACATGG 3630 0.0 22.129515 2 ACATGGG 3535 0.0 21.781492 3 GTACTTT 3790 0.0 20.189192 14-15 CATGGGG 1390 0.0 19.833757 4 >>END_MODULE