FastQCFastQC Report
Thu 26 May 2016
SRR936830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936830_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730667
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64290.8798809854557548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50550.6918336259883093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33420.457390302285446No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11600.15875905166101656No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9180.12563862881449414No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7950.10880469488836914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30000.042.137011
GTATCAA49750.037.5407371
CCTAGCG550.002579833134.5432133
GCCGTCC550.002582432234.536128
GCGTAAA702.0467877E-433.9449581
TATCAAC63250.029.2175452
ATCAACG63300.029.1134363
TCAACGC63800.028.8088574
CAACGCA64500.028.4962025
AACGCAG66200.027.832366
TAAAACG1351.3432436E-524.6263474
ACGCAGA74550.024.523897
CGCAGAG75000.024.1234828
GAGTACT46950.022.75898712-13
GCAGAGT78800.022.6588529
CGTAAAA1100.002874028621.5939432
AGTACTT48150.021.20548212-13
GTACATG34150.021.1522311
AGAGTAC66600.021.1410610-11
GTACTTT50450.021.13517214-15
Produced by FastQC (version 0.11.5)