##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936830_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 730667 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.263063748602306 33.0 31.0 34.0 31.0 34.0 2 32.330424119332065 33.0 31.0 34.0 31.0 34.0 3 32.455219682837736 34.0 31.0 34.0 31.0 34.0 4 36.12377457856999 37.0 35.0 37.0 35.0 37.0 5 36.05305973856764 37.0 35.0 37.0 35.0 37.0 6 36.089895944390534 37.0 35.0 37.0 35.0 37.0 7 36.047385471083274 37.0 35.0 37.0 35.0 37.0 8 36.03475180896359 37.0 35.0 37.0 35.0 37.0 9 37.742275208816054 39.0 37.0 39.0 35.0 39.0 10-11 37.64957429307742 39.0 37.0 39.0 35.0 39.0 12-13 37.57960055675157 39.0 37.0 39.0 35.0 39.0 14-15 38.80650693133808 40.0 38.0 41.0 35.0 41.0 16-17 38.73724418921341 40.0 38.0 41.0 35.0 41.0 18-19 38.80573640249251 40.0 38.0 41.0 35.0 41.0 20-21 38.90294758077209 40.0 38.0 41.0 35.0 41.0 22-23 38.84203816512857 40.0 38.0 41.0 35.0 41.0 24-25 38.75490065926065 40.0 38.0 41.0 35.0 41.0 26-27 38.58001661495592 40.0 38.0 41.0 34.0 41.0 28-29 38.43457826889677 40.0 38.0 41.0 34.0 41.0 30-31 38.329080141843 40.0 38.0 41.0 34.0 41.0 32-33 38.192255842948974 40.0 37.0 41.0 34.0 41.0 34-35 38.06393883944396 40.0 37.0 41.0 34.0 41.0 36-37 37.88484220582016 40.0 37.0 41.0 33.0 41.0 38-39 37.74677862829442 40.0 37.0 41.0 33.0 41.0 40-41 37.51135264080628 40.0 37.0 41.0 33.0 41.0 42-43 37.27134385431394 40.0 36.5 41.0 32.5 41.0 44-45 36.9617199079745 40.0 36.0 41.0 31.5 41.0 46-47 36.517087811547526 40.0 36.0 41.0 30.5 41.0 48-49 36.06278304617562 39.0 36.0 41.0 29.5 41.0 50-51 35.81114789637414 39.0 35.0 41.0 28.5 41.0 52-53 35.6023797434399 39.0 35.0 40.5 28.0 41.0 54-55 35.35600827736849 39.0 35.0 40.0 27.0 41.0 56-57 35.1188913691189 38.5 35.0 40.0 26.5 41.0 58-59 34.79604662589114 38.0 34.5 40.0 26.0 41.0 60-61 34.497812957202115 38.0 34.0 40.0 26.0 41.0 62-63 34.189427605188136 38.0 34.0 40.0 25.0 41.0 64-65 33.81109383617982 37.0 33.5 40.0 23.5 41.0 66-67 33.392970395542704 36.5 33.0 39.0 22.5 40.5 68-69 32.979437965584864 36.0 32.5 39.0 22.0 40.0 70-71 32.598303331066 36.0 32.0 39.0 21.0 40.0 72-73 32.213731426217414 35.0 32.0 38.0 19.5 40.0 74-75 31.742843183009498 35.0 31.0 37.5 18.0 39.5 76-77 30.27806374175924 33.5 29.5 36.0 14.0 38.5 78-79 30.94613346435517 34.5 31.0 36.0 14.0 39.0 80-81 30.596567246091585 34.5 31.0 36.0 7.5 38.0 82-83 30.406542925847205 34.0 31.0 35.5 7.5 37.0 84-85 30.079337783148823 34.0 30.5 35.0 6.0 37.0 86-87 29.721892462640298 34.0 30.0 35.0 2.0 36.0 88-89 29.3472163105765 34.0 30.0 35.0 2.0 36.0 90-91 28.922500947764167 34.0 29.0 35.0 2.0 36.0 92-93 28.51820117782793 34.0 29.0 35.0 2.0 35.0 94-95 28.06039002719433 34.0 29.0 35.0 2.0 35.0 96-97 27.46743318091552 33.5 27.0 35.0 2.0 35.0 98-99 26.755244865308 33.0 26.5 35.0 2.0 35.0 100-101 23.672360322828318 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3249509134346056 1101 2 0.20938923385935482 1101 3 0.18652011130681245 1101 4 0.15906201345357118 1101 5 0.2758201859317637 1101 6 0.34061890271385664 1101 7 0.26318993697369564 1101 8 0.3447362526253812 1101 9 0.3677635598527118 1101 10-11 0.3728889306714649 1101 12-13 0.3457275394959467 1101 14-15 0.4315283092692468 1101 16-17 0.41325658727769365 1101 18-19 0.4849254795188571 1101 20-21 0.3155716035253491 1101 22-23 0.395970449910088 1101 24-25 0.40896164640367516 1101 26-27 0.5231396915119291 1101 28-29 0.5503176958811977 1101 30-31 0.4990140402744814 1101 32-33 0.4626375665604243 1101 34-35 0.545044051027503 1101 36-37 0.40145105202161346 1101 38-39 0.49031025454532795 1101 40-41 0.43430158191019075 1101 42-43 0.43666326165303104 1101 44-45 0.41331571721182314 1101 46-47 0.5770617247036682 1101 48-49 0.5126010625462385 1101 50-51 0.5342847733892739 1101 52-53 0.5076977207088191 1101 54-55 0.5007844417331313 1101 56-57 0.5586501280840537 1101 58-59 0.6192204708413342 1101 60-61 0.6282868003480573 1101 62-63 0.5497148799853875 1101 64-65 0.5104657854675594 1101 66-67 0.6258710313018625 1101 68-69 0.49510723723302874 1101 70-71 0.6286355724360746 1101 72-73 0.5349825558088099 1101 74-75 0.3976505588656103 1101 76-77 0.3780652746984181 1101 78-79 0.5594878632193598 1101 80-81 0.8547537424693559 1101 82-83 0.6678772577239158 1101 84-85 0.5227405198365105 1101 86-87 0.41473444974255713 1101 88-89 0.40294717136365676 1101 90-91 0.5941017853915689 1101 92-93 0.7044285473847403 1101 94-95 0.4496873120091429 1101 96-97 0.8142129271681426 1101 98-99 1.1107146206264389 1101 100-101 1.255737252392624 1102 1 0.23568714116346712 1102 2 0.23306832458226978 1102 3 0.15642846682595746 1102 4 0.15497470373959743 1102 5 0.14547425788206425 1102 6 0.13835680291182229 1102 7 0.2351376808469965 1102 8 0.23276188577440138 1102 9 0.24539172559781974 1102 10-11 0.3393563516558302 1102 12-13 0.2942607158219701 1102 14-15 0.3692075023882282 1102 16-17 0.43884707126976963 1102 18-19 0.36277976991410554 1102 20-21 0.22371593078317886 1102 22-23 0.23417826273007591 1102 24-25 0.08368987017322382 1102 26-27 0.3813766018812359 1102 28-29 0.48462848501355893 1102 30-31 0.4421884890423087 1102 32-33 0.3715242653112014 1102 34-35 0.3271217525770993 1102 36-37 0.5377978163058614 1102 38-39 0.4923240438824834 1102 40-41 0.42317880902466953 1102 42-43 0.24288510273434127 1102 44-45 0.37942901846104604 1102 46-47 0.23369144345580395 1102 48-49 0.33977996554303047 1102 50-51 0.25533441841623983 1102 52-53 0.17965911739118212 1102 54-55 0.2625535897978466 1102 56-57 0.2268762015361645 1102 58-59 0.2582295328933526 1102 60-61 0.5070413228410544 1102 62-63 0.2458844137419547 1102 64-65 0.20731155992315564 1102 66-67 0.029463000036933806 1102 68-69 0.0875388878576473 1102 70-71 0.2892267390686101 1102 72-73 0.2598144555971871 1102 74-75 0.0043323289523051756 1102 76-77 0.19838577439122673 1102 78-79 0.2937423345143273 1102 80-81 0.23384318543474691 1102 82-83 0.35263602965373764 1102 84-85 0.1143039371346184 1102 86-87 0.2066080417966205 1102 88-89 0.050280180442772604 1102 90-91 0.19115405858427792 1102 92-93 0.18766972425688166 1102 94-95 0.29443140716922045 1102 96-97 0.39115503925770767 1102 98-99 0.09311529644048733 1102 100-101 0.2450880613215638 1103 1 0.055036651302444284 1103 2 -0.03917478318714984 1103 3 0.09566576461588738 1103 4 0.004228520098678246 1103 5 0.010976080620729078 1103 6 0.07699601457735383 1103 7 -0.009693146220669746 1103 8 0.06274878484024526 1103 9 0.08877860187943298 1103 10-11 8.324851714434089E-4 1103 12-13 0.13863016216510005 1103 14-15 0.24328189290999092 1103 16-17 0.24637726037891383 1103 18-19 0.19194074052244758 1103 20-21 0.058608560083705186 1103 22-23 0.1684955901395071 1103 24-25 0.10306358856009723 1103 26-27 0.18348129543330316 1103 28-29 0.13971079305638767 1103 30-31 0.16540277475659337 1103 32-33 0.2301409775463341 1103 34-35 0.3639396495923535 1103 36-37 0.4086183335079596 1103 38-39 0.24619736354527078 1103 40-41 0.32561957001806263 1103 42-43 0.2140416873777653 1103 44-45 0.305985466399072 1103 46-47 0.37009068018398494 1103 48-49 0.5969071585254895 1103 50-51 0.5480192555089687 1103 52-53 0.43300555165867394 1103 54-55 0.4582060732792783 1103 56-57 0.518008862734618 1103 58-59 0.553919040753911 1103 60-61 0.6508098350306213 1103 62-63 0.6590622446829428 1103 64-65 0.5839135763278023 1103 66-67 0.522179165450467 1103 68-69 0.4633576962422481 1103 70-71 0.3974233670020766 1103 72-73 0.6780902587414133 1103 74-75 0.7709576991960994 1103 76-77 0.8426099712470076 1103 78-79 0.6554776386838412 1103 80-81 1.044071882176599 1103 82-83 0.9016434914901481 1103 84-85 0.9113676190667235 1103 86-87 0.8376672238426437 1103 88-89 0.7235756349882294 1103 90-91 0.8405118280078376 1103 92-93 0.9326571929285592 1103 94-95 1.0287316464036707 1103 96-97 1.2482124555256497 1103 98-99 1.2801311314826336 1103 100-101 0.9839237869993944 1104 1 0.2018934177244347 1104 2 0.20005917126419348 1104 3 0.1038908728409993 1104 4 0.24482705200156119 1104 5 0.18186823194766788 1104 6 0.2477375918406679 1104 7 0.10438552872756901 1104 8 0.13533489438287916 1104 9 0.29808596118679276 1104 10-11 0.13585507128098584 1104 12-13 0.12328572399457727 1104 14-15 0.18727304668374956 1104 16-17 0.20335072170019686 1104 18-19 0.2796030781847847 1104 20-21 0.2484401053500349 1104 22-23 0.26170564095825455 1104 24-25 0.2576608029399168 1104 26-27 0.35622268447903593 1104 28-29 0.41064529333871747 1104 30-31 0.5012205801396235 1104 32-33 0.5028682502736004 1104 34-35 0.5618927834150611 1104 36-37 0.44325507357948624 1104 38-39 0.4887580637146769 1104 40-41 0.559150531809891 1104 42-43 0.41485102296970666 1104 44-45 0.7086440397098173 1104 46-47 0.6324989241139676 1104 48-49 0.5887291046952612 1104 50-51 0.4920668745565919 1104 52-53 0.5572620922195597 1104 54-55 0.4270701801868597 1104 56-57 0.27245550305082133 1104 58-59 0.3129676008894293 1104 60-61 0.4045459539841332 1104 62-63 0.32926269640513084 1104 64-65 0.37342044626945636 1104 66-67 0.3458851695912557 1104 68-69 0.3157527649416636 1104 70-71 0.25934224522530513 1104 72-73 0.47999125629458916 1104 74-75 0.5677674112231976 1104 76-77 0.5446427211058449 1104 78-79 0.685818312502775 1104 80-81 0.9969704329012394 1104 82-83 0.7834052080344733 1104 84-85 0.5496981291363632 1104 86-87 0.6311360738332326 1104 88-89 0.3509659982481601 1104 90-91 0.5947561344260599 1104 92-93 0.5349346256407763 1104 94-95 0.42085003526118925 1104 96-97 0.546946692303365 1104 98-99 1.1154878037958298 1104 100-101 1.8706356258851464 1105 1 0.19077557362673758 1105 2 0.09351975804397483 1105 3 0.029868418330735835 1105 4 0.05022859048899164 1105 5 0.08044252203839619 1105 6 0.09650293042572855 1105 7 0.07625342753078002 1105 8 -0.04930379672453 1105 9 -0.01774834001336245 1105 10-11 0.12735467571801706 1105 12-13 0.09627718993407797 1105 14-15 0.16378923110026022 1105 16-17 0.09292637522070635 1105 18-19 0.2583100081112306 1105 20-21 0.18524513489101224 1105 22-23 -0.03341462701461495 1105 24-25 0.07682119951282829 1105 26-27 0.2548878698886554 1105 28-29 0.09331037775353934 1105 30-31 0.16220881424556666 1105 32-33 0.2854853621590152 1105 34-35 0.28066350838938803 1105 36-37 0.24544918558271434 1105 38-39 0.1655696579175654 1105 40-41 0.27358353017714876 1105 42-43 0.09612911214811248 1105 44-45 0.013570902643046168 1105 46-47 0.36651397233898564 1105 48-49 0.448533174785652 1105 50-51 0.16672812116905789 1105 52-53 0.3719551521936424 1105 54-55 0.4653286932067786 1105 56-57 0.5309474823805544 1105 58-59 0.3996278712188399 1105 60-61 0.2343983332532673 1105 62-63 0.2838158785829208 1105 64-65 0.3794473108859293 1105 66-67 0.26704947129638157 1105 68-69 0.4436088136884848 1105 70-71 0.5448250845257405 1105 72-73 0.11881281531763221 1105 74-75 0.06734491631501882 1105 76-77 -0.08862749047825957 1105 78-79 0.23035533551276188 1105 80-81 0.3873742437716423 1105 82-83 0.47275530650440345 1105 84-85 -0.08564079257583401 1105 86-87 0.10405936828357198 1105 88-89 -0.05989935885261488 1105 90-91 -0.2896662594873227 1105 92-93 0.22040399896804885 1105 94-95 0.2691541642652133 1105 96-97 0.21757067183508383 1105 98-99 0.31786033994355023 1105 100-101 0.1760893450186103 1106 1 0.014345860611655326 1106 2 0.17134617733380964 1106 3 -0.02158735263722633 1106 4 -0.016584000713841363 1106 5 -0.0014836818390264739 1106 6 -0.0237458361645011 1106 7 0.04484490831737986 1106 8 0.07907505116651237 1106 9 0.08148034458117337 1106 10-11 -0.016867407528451395 1106 12-13 0.05679070532564623 1106 14-15 0.16759369222179288 1106 16-17 0.2611217751234207 1106 18-19 0.20888675746846985 1106 20-21 0.00403813051327262 1106 22-23 0.08544438208830485 1106 24-25 -0.05345119295468237 1106 26-27 0.006329743281746403 1106 28-29 0.0741143524599508 1106 30-31 0.16886227821610333 1106 32-33 0.22325897066432532 1106 34-35 0.20341036406307467 1106 36-37 0.32388362377324853 1106 38-39 0.446196438544348 1106 40-41 0.6240803684788645 1106 42-43 0.4881102114462905 1106 44-45 0.6601729455865595 1106 46-47 0.7991107342040422 1106 48-49 0.6766792382975666 1106 50-51 0.42098425347396784 1106 52-53 0.5495140431671643 1106 54-55 0.721669461742664 1106 56-57 0.5636067833325313 1106 58-59 0.8261791880140592 1106 60-61 0.5508634850842711 1106 62-63 0.4975432081639113 1106 64-65 0.5379317803460069 1106 66-67 0.5309296742009764 1106 68-69 0.5832794411639952 1106 70-71 0.7076178021965092 1106 72-73 0.59881767946883 1106 74-75 0.7513661796045774 1106 76-77 0.5030791317161629 1106 78-79 0.7673750005812039 1106 80-81 1.0584186465233678 1106 82-83 0.9378202776669369 1106 84-85 0.7788057365048395 1106 86-87 0.7251593613347822 1106 88-89 0.6212381576507546 1106 90-91 0.6827991702951799 1106 92-93 0.9306221908935584 1106 94-95 1.1574871501591737 1106 96-97 1.1669834992966948 1106 98-99 0.7654143667658708 1106 100-101 1.3505386536142616 1107 1 0.06108631325098912 1107 2 0.11959996266624273 1107 3 -0.01059548349929429 1107 4 0.011469874250146006 1107 5 0.12572509565064394 1107 6 0.054025539359685126 1107 7 -0.02086222059199372 1107 8 0.10851341347004251 1107 9 0.06445835284007728 1107 10-11 0.09792499939878496 1107 12-13 0.20326426401830844 1107 14-15 0.23286066619662904 1107 16-17 0.23618099596915698 1107 18-19 0.1182868663067822 1107 20-21 0.2489783494703488 1107 22-23 0.19767988898672684 1107 24-25 0.1065991447641963 1107 26-27 0.12541366082071903 1107 28-29 0.04909354513576858 1107 30-31 0.15754429428575634 1107 32-33 0.22798863550634962 1107 34-35 0.2847589987824932 1107 36-37 0.2038053562230715 1107 38-39 0.24726441447187852 1107 40-41 0.3860705890364997 1107 42-43 0.261642494810026 1107 44-45 0.31439723253556195 1107 46-47 0.32327724081437026 1107 48-49 0.3492479824955268 1107 50-51 0.2709994588262461 1107 52-53 0.4733832451262572 1107 54-55 0.6770457541976143 1107 56-57 0.5680143347906466 1107 58-59 0.5452823123138089 1107 60-61 0.44940777267350285 1107 62-63 0.6331429392692556 1107 64-65 0.5710694448038964 1107 66-67 0.7015197218977676 1107 68-69 0.7058305791645694 1107 70-71 0.3626423708446822 1107 72-73 0.46939212167249167 1107 74-75 0.6604221673122446 1107 76-77 0.8099691570050673 1107 78-79 0.8121547630575954 1107 80-81 0.936293658527589 1107 82-83 0.991613212780095 1107 84-85 1.1876456481537438 1107 86-87 0.983090152973439 1107 88-89 0.6178129519783546 1107 90-91 0.8557940644979389 1107 92-93 0.9561991939986498 1107 94-95 0.970615371293011 1107 96-97 0.9152408858967753 1107 98-99 1.052089276334037 1107 100-101 1.0109071033085506 1108 1 0.02692685549353513 1108 2 -0.03864802381083621 1108 3 -0.0023880989246976014 1108 4 0.08881649849196549 1108 5 0.07672522822828398 1108 6 0.01829586516923598 1108 7 -0.02022833374700639 1108 8 0.01933423478853058 1108 9 0.10284786594869644 1108 10-11 -0.010904671477220518 1108 12-13 -0.08641580956228267 1108 14-15 -0.1061942209466551 1108 16-17 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-0.6958445467873631 2316 84-85 -1.1781459375520988 2316 86-87 -1.1968224093311974 2316 88-89 -0.9902116741938158 2316 90-91 -1.0792876707390278 2316 92-93 -1.321415488775699 2316 94-95 -1.2036105727806579 2316 96-97 -1.6030492213939205 2316 98-99 -1.693538838897858 2316 100-101 -2.2238969010844514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 5.0 5 18.0 6 30.0 7 95.0 8 198.0 9 372.0 10 667.0 11 1135.0 12 1817.0 13 2450.0 14 3252.0 15 4521.0 16 6637.0 17 9745.0 18 8934.0 19 4785.0 20 3687.0 21 3806.0 22 3788.0 23 4064.0 24 4620.0 25 5326.0 26 6301.0 27 7930.0 28 9889.0 29 12245.0 30 15172.0 31 19155.0 32 24817.0 33 32472.0 34 45338.0 35 65399.0 36 100453.0 37 164930.0 38 143588.0 39 13023.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.43017880195435 20.157486998150514 16.003466247212423 21.408867952682716 2 15.102709538956807 21.361229886504866 41.04384865125294 22.492211923285392 3 18.596877844467105 26.197909894125942 31.552522978161335 23.652689283245618 4 12.343926850398335 18.372391253471143 38.84916110895934 30.434520787171177 5 12.805797736200267 37.98406854358568 34.65310759207807 14.557026128135991 6 29.319512171755395 38.16526543555409 18.131378589699548 14.383843802990967 7 26.332789081756808 34.34587849184375 21.843603173538696 17.477729252860744 8 25.540979792380913 34.88370183326148 20.47272005638699 19.102598317970614 9 25.99405748446283 17.084800599999724 21.431924529231512 35.48921738630594 10-11 23.562580491523498 27.790703562635237 28.10397896716288 20.542736978678384 12-13 25.40067924378529 25.442969457296122 28.74776488009903 20.408586418819556 14-15 22.451951436153543 26.189426920881882 28.137030959383686 23.221590683580892 16-17 20.450150342084697 29.566752022467142 28.536118368559137 21.44697926688902 18-19 20.14193570463139 27.292511420212495 32.42334342760355 20.142209447552567 20-21 21.214535003079448 26.226579073427768 32.70450968315883 19.85437624033395 22-23 20.831240496696854 26.381237964763706 32.613830924347205 20.17369061419224 24-25 20.693423813440894 26.414105261140865 32.76445653765769 20.128014387760548 26-27 20.625142003071375 26.564429017626175 32.677609913799 20.13281906550345 28-29 20.33965350278368 26.73147663385106 32.731291853585596 20.197578009779665 30-31 20.629138687490546 26.510670137921448 32.64378312918147 20.21640804540653 32-33 20.464609566875815 26.58396934811365 32.550795072650416 20.400626012360117 34-35 20.701430700732665 26.64240420135748 32.537389426255196 20.11877567165466 36-37 20.66474107130635 26.385196643440885 32.589150045858375 20.360912239394395 38-39 20.494596869674716 26.716375009410136 32.61608688808437 20.172941232830773 40-41 20.5304494461995 26.693430497079063 32.54501853301724 20.231101523704194 42-43 20.616043688323778 26.70873082134596 32.05521262472113 20.620012865609134 44-45 20.757054012328783 26.850275371998556 31.68988350067338 20.70278711499929 46-47 20.83005429404736 27.224899438993443 31.277791996452482 20.667254270506714 48-49 20.98102656117199 27.514428873897735 30.766519811730053 20.738024753200218 50-51 20.944691031573644 27.660848487834656 30.495414300593588 20.899046179998113 52-53 21.13594977612354 27.916980600110726 29.981653284718984 20.96541633904675 54-55 20.908046393893713 27.720960533438078 30.241570423311092 21.129422649357117 56-57 21.14727152288877 27.980302847820614 29.89999507297472 20.9724305563159 58-59 21.141487736096312 27.735932077893903 30.173110493861376 20.949469692148405 60-61 21.09640917134618 27.559613339592453 30.5774723642918 20.76650512476956 62-63 20.978737206877014 27.685084566683 30.546720389343424 20.789457837096567 64-65 20.949540600059947 27.707366412683896 30.26345864579327 21.07963434146288 66-67 20.786246973630725 28.05425498281693 29.975392144920832 21.184105898631515 68-69 20.886321955492296 27.679289957025155 30.282826485643117 21.151561601839433 70-71 21.004386535184665 27.83567943392482 30.198660088004438 20.96127394288608 72-73 20.974699371539206 27.68957335430256 30.04635266211284 21.289374612045396 74-75 20.981802827812228 27.856639870359434 29.85476342170617 21.306793880122168 76-77 20.847613051571226 27.946868498850325 29.83671849337567 21.368799956202782 78-79 20.806223450450766 27.96193221519823 29.743228603125004 21.488615731225998 80-81 21.054176674673073 28.00229927531769 29.55143602061136 21.392088029397875 82-83 20.893149694993014 28.099444458891465 29.753270767095653 21.25413507901987 84-85 20.698796697762013 28.169754302982525 29.523948342311567 21.6075006569439 86-87 20.697390192796444 27.909978143258147 29.574689975050195 21.817941688895214 88-89 20.73335970251187 27.671145321090368 29.525998048315817 22.069496928081943 90-91 20.609503319257026 27.587025711667728 29.91779355802045 21.885677411054793 92-93 20.602245487162048 27.427256384761563 29.973428333485018 21.99706979459137 94-95 20.381544929864877 27.5493012491429 29.967933145466912 22.10122067552531 96-97 20.50143293926363 27.455841656789833 29.927217086876905 22.115508317069633 98-99 20.29767403666523 27.71947472507921 29.564985321590605 22.417865916664955 100-101 19.855993408708006 27.840407521408693 29.641926967499295 22.661672102384006 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 640.0 1 442.0 2 284.5 3 328.0 4 275.5 5 259.5 6 480.0 7 1066.0 8 1824.0 9 2208.0 10 1852.0 11 1404.0 12 1411.0 13 1518.5 14 1569.0 15 1646.5 16 1727.0 17 1826.0 18 1919.5 19 2014.5 20 2184.0 21 2421.0 22 2811.5 23 3351.5 24 3906.0 25 4680.5 26 5751.5 27 6993.5 28 8022.0 29 9183.5 30 10950.0 31 12549.0 32 14073.0 33 15841.0 34 18210.5 35 20180.0 36 22010.0 37 24002.5 38 25027.0 39 25126.5 40 25158.0 41 25889.5 42 25906.0 43 26078.5 44 26920.0 45 27293.5 46 26857.5 47 26586.5 48 27855.5 49 27169.5 50 24686.0 51 22833.0 52 20833.0 53 19465.0 54 17995.5 55 16112.0 56 14474.0 57 12722.0 58 11254.0 59 9948.5 60 8274.5 61 6432.0 62 4928.5 63 3772.0 64 2703.0 65 1936.5 66 1414.0 67 1086.5 68 791.0 69 489.0 70 337.0 71 245.0 72 175.0 73 122.0 74 86.5 75 69.0 76 51.0 77 23.5 78 12.0 79 7.5 80 7.0 81 7.0 82 5.0 83 2.5 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026551082777790704 2 0.008622258840210383 3 0.011222622617416689 4 0.0 5 0.0050638663029807015 6 0.0 7 0.0 8 2.7372250286382166E-4 9 0.0 10-11 0.0 12-13 2.0529187714786627E-4 14-15 0.0 16-17 0.0 18-19 0.007458938203039141 20-21 0.0023266412743424844 22-23 0.0 24-25 0.006295617565867899 26-27 0.006706201320163631 28-29 0.009511856974517802 30-31 0.03934760978667436 32-33 0.0014370431400350639 34-35 0.006295617565867899 36-37 0.021487216474810004 38-39 0.00985401010309758 40-41 0.010812038863120958 42-43 0.0050638663029807015 44-45 0.0026003637772063063 46-47 0.0021897800229105733 48-49 0.0019160575200467516 50-51 0.003558392537229682 52-53 0.0025319331514903507 54-55 0.0014370431400350639 56-57 4.1058375429573255E-4 58-59 8.895981343074205E-4 60-61 0.0 62-63 1.3686125143191083E-4 64-65 0.005474450057276434 66-67 8.211675085914651E-4 68-69 9.580287600233758E-4 70-71 0.0030109475315020385 72-73 0.009922440728813535 74-75 0.0043111294201051914 76-77 0.0036952537886615925 78-79 0.010333024483109268 80-81 2.7372250286382166E-4 82-83 0.0038321150400935033 84-85 0.0015054737657510192 86-87 0.0 88-89 0.0021897800229105733 90-91 8.895981343074205E-4 92-93 2.0529187714786627E-4 94-95 8.211675085914651E-4 96-97 1.3686125143191083E-4 98-99 2.7372250286382166E-4 100-101 8.211675085914651E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 730667.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.2634229613639 #Duplication Level Percentage of deduplicated Percentage of total 1 84.34037024341448 55.88681626157405 2 9.727522619213213 12.89157891366319 3 2.5679599026377407 5.104854395289224 4 1.0655428616489333 2.8242606929962126 5 0.5532814082056008 1.833115998429338 6 0.36282839375379483 1.4425350790619995 7 0.22636768759258089 1.0499928479413152 8 0.17072923486386565 0.9050482801323492 9 0.13121746634594159 0.7825426625159695 >10 0.7749833694356993 9.576953100644008 >50 0.05425323022369425 2.460572449988302 >100 0.023488540343098812 2.704471178562818 >500 6.235895662791092E-4 0.3353902377153774 >1k 4.1572637751940625E-4 0.6200931622975889 >5k 4.1572637751940625E-4 1.5817747391882524 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6429 0.8798809854557548 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5055 0.6918336259883093 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3342 0.457390302285446 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1160 0.15875905166101656 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 918 0.12563862881449414 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 795 0.10880469488836914 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.3686125143191083E-4 0.0 0.0 0.0 0.0 8 1.3686125143191083E-4 0.0 0.0 0.0 0.0 9 1.3686125143191083E-4 0.0 0.0 0.0 0.0 10-11 1.3686125143191083E-4 0.0 0.0 0.0 0.0 12-13 1.3686125143191083E-4 0.0 0.0 0.0 0.0 14-15 1.3686125143191083E-4 0.0 0.0 0.0 0.0 16-17 1.3686125143191083E-4 0.0 0.0 6.843062571595542E-5 0.0 18-19 1.3686125143191083E-4 0.0 0.0 1.3686125143191083E-4 0.0 20-21 1.3686125143191083E-4 0.0 0.0 1.3686125143191083E-4 0.0 22-23 1.3686125143191083E-4 0.0 0.0 2.7372250286382166E-4 0.0 24-25 1.3686125143191083E-4 0.0 0.0 4.1058375429573255E-4 0.0 26-27 1.3686125143191083E-4 0.0 0.0 8.21167508591465E-4 0.0 28-29 3.4215312857977713E-4 0.0 0.0 0.0028740862800701273 0.0 30-31 4.1058375429573255E-4 0.0 0.0 0.005885033811572167 0.0 32-33 4.1058375429573255E-4 0.0 0.0 0.009238134471653982 0.0 34-35 5.474450057276433E-4 0.0 0.0 0.013549263891759172 0.0 36-37 6.843062571595543E-4 0.0 0.0 0.016833933926125033 0.0 38-39 8.211675085914651E-4 0.0 0.0 0.02169250835195787 0.0 40-41 8.211675085914651E-4 0.0 0.0 0.02641422152635879 0.0 42-43 8.895981343074206E-4 0.0 0.0 0.03989505479240201 0.0 44-45 9.580287600233759E-4 0.0 0.0 0.054197055567036696 0.0 46-47 9.580287600233759E-4 0.0 0.0 0.06350362066440662 0.0 48-49 9.580287600233759E-4 0.0 0.0 0.07725817643331367 0.0 50-51 9.580287600233759E-4 0.0 0.0 0.09463955536516636 0.0 52-53 9.580287600233759E-4 0.0 0.0 0.1360400839233194 0.0 54-55 9.580287600233759E-4 0.0 0.0 0.16970795177556947 0.0 56-57 0.0010948900114552866 0.0 0.0 0.23499076870859092 0.0 58-59 0.0010948900114552866 0.0 0.0 0.308485260727527 0.0 60-61 0.0010948900114552866 0.0 0.0 0.38848066218947896 0.0 62-63 0.0011633206371712422 0.0 0.0 0.5011174721179416 0.0 64-65 0.0012317512628871976 0.0 0.0 0.5527825945334879 0.0 66-67 0.0012317512628871976 0.0 0.0 0.6869750515624764 0.0 68-69 0.0012317512628871976 0.0 0.0 0.8725589085041476 0.0 70-71 0.0012317512628871976 0.0 0.0 0.9888909722212718 0.0 72-73 0.0013686125143191085 0.0 0.0 1.1984255481635273 0.0 74-75 0.0015054737657510192 0.0 0.0 1.4096024591229657 0.0 76-77 0.0015054737657510192 0.0 0.0 1.5309983891430707 0.0 78-79 0.0015054737657510192 0.0 0.0 1.6421297253057823 0.0 80-81 0.0015054737657510192 0.0 0.0 1.8729462258457 0.0 82-83 0.0015054737657510192 0.0 0.0 2.0543558146186975 0.0 84-85 0.0015739043914669748 0.0 0.0 2.371668626063583 0.0 86-87 0.0016423350171829302 0.0 0.0 2.8581419442783105 0.0 88-89 0.0016423350171829302 0.0 0.0 3.4913305240280454 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3000 0.0 42.13701 1 GTATCAA 4975 0.0 37.540737 1 CCTAGCG 55 0.0025798331 34.543213 3 GCCGTCC 55 0.0025824322 34.53612 8 GCGTAAA 70 2.0467877E-4 33.944958 1 TATCAAC 6325 0.0 29.217545 2 ATCAACG 6330 0.0 29.113436 3 TCAACGC 6380 0.0 28.808857 4 CAACGCA 6450 0.0 28.496202 5 AACGCAG 6620 0.0 27.83236 6 TAAAACG 135 1.3432436E-5 24.626347 4 ACGCAGA 7455 0.0 24.52389 7 CGCAGAG 7500 0.0 24.123482 8 GAGTACT 4695 0.0 22.758987 12-13 GCAGAGT 7880 0.0 22.658852 9 CGTAAAA 110 0.0028740286 21.593943 2 AGTACTT 4815 0.0 21.205482 12-13 GTACATG 3415 0.0 21.152231 1 AGAGTAC 6660 0.0 21.14106 10-11 GTACTTT 5045 0.0 21.135172 14-15 >>END_MODULE