FastQCFastQC Report
Thu 26 May 2016
SRR936829_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936829_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774984
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47170.6086577271272697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34590.446331795237063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20920.26994105684762526No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7880.101679518544899No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.10013109948076347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31300.035.5304681
GTATCAA48200.033.1300161
ATCAACG58850.027.2187983
TCAACGC59250.026.954824
TATCAAC60000.026.6178862
CAACGCA60050.026.5165675
AACGCAG62350.025.6859046
GAGTACT34450.023.1713512-13
ACGCAGA68600.022.860797
CGCAGAG69100.022.6265968
AGTACTT35650.022.19146512-13
GTACATG38300.021.8395331
GCAGAGT70850.021.7966029
TACATGG38600.021.5494542
GTACTTT37850.020.96098914-15
AGAGTAC57350.020.54771610-11
ACATGGG38400.020.547663
TACCTGG5350.020.4342922
ATGGGCG1404.827838E-420.370835
TATACAG6150.020.0948035
Produced by FastQC (version 0.11.5)