##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936829_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 774984 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.884167931208903 33.0 31.0 34.0 30.0 34.0 2 32.00332522994023 33.0 31.0 34.0 30.0 34.0 3 32.0002825864792 33.0 31.0 34.0 30.0 34.0 4 35.56130707214601 37.0 35.0 37.0 33.0 37.0 5 35.55798571325344 37.0 35.0 37.0 33.0 37.0 6 35.67686687725166 37.0 35.0 37.0 33.0 37.0 7 35.63441954930683 37.0 35.0 37.0 33.0 37.0 8 35.5896057208923 37.0 35.0 37.0 33.0 37.0 9 37.21384054380478 39.0 37.0 39.0 34.0 39.0 10-11 37.104260862159734 39.0 37.0 39.0 33.0 39.0 12-13 37.01221057466993 39.0 37.0 39.0 33.0 39.0 14-15 38.200513171884836 40.0 38.0 41.0 33.0 41.0 16-17 38.13624861932634 40.0 38.0 41.0 33.0 41.0 18-19 38.10707382345958 40.0 38.0 41.0 33.0 41.0 20-21 38.01856425422976 40.0 38.0 41.0 33.0 41.0 22-23 37.89513525440525 40.0 38.0 41.0 33.0 41.0 24-25 38.00599109142898 40.0 38.0 41.0 33.0 41.0 26-27 37.95455970703911 40.0 38.0 41.0 33.0 41.0 28-29 37.87418772516594 40.0 38.0 41.0 33.0 41.0 30-31 37.68300377814251 40.0 37.0 41.0 33.0 41.0 32-33 37.47453753883951 40.0 37.0 41.0 32.5 41.0 34-35 37.29760420860302 40.0 36.5 41.0 32.0 41.0 36-37 37.113616539180164 40.0 36.0 41.0 31.0 41.0 38-39 36.95866237238447 39.0 36.0 41.0 31.0 41.0 40-41 36.778926403641876 39.0 36.0 41.0 30.5 41.0 42-43 36.54695967916757 39.0 35.0 41.0 30.0 41.0 44-45 36.271493992134026 39.0 35.0 41.0 30.0 41.0 46-47 35.89113323629907 39.0 35.0 40.5 28.5 41.0 48-49 35.618535995581844 39.0 35.0 40.5 27.0 41.0 50-51 34.89470492294035 38.0 34.0 40.0 26.5 40.5 52-53 35.091001233573856 38.5 35.0 40.0 27.0 40.5 54-55 35.504980747989634 39.0 35.0 40.5 27.0 41.0 56-57 35.41380660762029 39.0 35.0 41.0 27.0 41.0 58-59 35.18828517750043 39.0 35.0 41.0 26.0 41.0 60-61 34.93673482291247 38.5 35.0 40.0 26.0 41.0 62-63 34.621319278849626 38.0 34.0 40.0 25.5 41.0 64-65 34.30966897381107 38.0 34.0 40.0 24.5 41.0 66-67 33.94870410227824 37.0 34.0 40.0 23.5 41.0 68-69 33.56342905143848 36.5 33.5 39.5 22.5 41.0 70-71 33.179884358902896 36.0 33.0 39.0 22.0 41.0 72-73 32.76823650552785 36.0 33.0 39.0 20.5 40.0 74-75 32.32649770833979 35.0 32.0 38.0 19.5 40.0 76-77 31.89065967813529 35.0 32.0 37.0 18.0 39.0 78-79 31.456033931023093 35.0 31.5 37.0 16.5 39.0 80-81 31.00346716835444 35.0 31.0 36.0 12.0 39.0 82-83 30.581729429252732 35.0 31.0 36.0 7.5 37.0 84-85 30.18834388838995 34.0 31.0 35.0 6.5 37.0 86-87 29.83045198352482 34.0 30.0 35.0 2.0 36.5 88-89 29.471295536423977 34.0 30.0 35.0 2.0 36.0 90-91 29.087690197475045 34.0 29.0 35.0 2.0 36.0 92-93 28.734844203235163 34.0 29.0 35.0 2.0 35.5 94-95 28.350883373076087 34.0 29.0 35.0 2.0 35.0 96-97 27.867888240273345 34.0 29.0 35.0 2.0 35.0 98-99 27.254975586592757 34.0 27.0 35.0 2.0 35.0 100-101 24.78004126536806 31.5 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3473722798029293 1101 2 0.44127633725225124 1101 3 0.32719549854663654 1101 4 0.15309280900434885 1101 5 0.3951644337764222 1101 6 0.19490365151932565 1101 7 0.29294820594037674 1101 8 0.21664283145712915 1101 9 0.30245818378526934 1101 10-11 0.32630085259952324 1101 12-13 0.48019349596472694 1101 14-15 0.42431127104939037 1101 16-17 0.40165581520963656 1101 18-19 0.6785623198315633 1101 20-21 0.5985827447979091 1101 22-23 0.7057475537169395 1101 24-25 0.6030960403155063 1101 26-27 0.642906610549808 1101 28-29 0.6909301417907585 1101 30-31 0.5906632365314408 1101 32-33 0.6532052976862843 1101 34-35 0.7236553162932182 1101 36-37 0.5904376768555935 1101 38-39 0.7866437143702143 1101 40-41 0.6178257892437387 1101 42-43 0.5796437558465186 1101 44-45 0.5142583848778628 1101 46-47 0.6395429017094969 1101 48-49 0.5960466625664651 1101 50-51 0.7953689398782089 1101 52-53 0.7478277978173011 1101 54-55 0.9766739108364746 1101 56-57 0.844621303215213 1101 58-59 0.7057391947887197 1101 60-61 0.9309183053606063 1101 62-63 1.0188479596462798 1101 64-65 1.2206385478201796 1101 66-67 1.102321317001575 1101 68-69 1.1984817105691903 1101 70-71 1.1354797018556013 1101 72-73 1.1722952340470414 1101 74-75 1.0720282122315794 1101 76-77 1.0254384249116377 1101 78-79 0.9162105308137249 1101 80-81 0.7686866872457081 1101 82-83 0.7858366123610416 1101 84-85 0.840576150671847 1101 86-87 0.889665169129497 1101 88-89 0.5555638447785185 1101 90-91 0.6177751285543387 1101 92-93 0.7193673378514553 1101 94-95 0.8123337711952772 1101 96-97 0.6467999201199781 1101 98-99 0.47473495797400034 1101 100-101 0.898835539255419 1102 1 0.13537621293960456 1102 2 0.21445234511852718 1102 3 0.04067632450533765 1102 4 0.041372061708379704 1102 5 -0.02045011882928094 1102 6 0.108168154960822 1102 7 0.07383316169259047 1102 8 0.06885522083766205 1102 9 0.25248768231034546 1102 10-11 0.10295768642449588 1102 12-13 -0.005258814753069885 1102 14-15 0.04225129071507183 1102 16-17 0.14327331766784823 1102 18-19 0.1369743158984349 1102 20-21 0.1419308273937787 1102 22-23 0.17542306994113233 1102 24-25 -0.0584280501466381 1102 26-27 0.11320159580053968 1102 28-29 0.08085639547807233 1102 30-31 -0.07581660614308561 1102 32-33 0.005752003684321494 1102 34-35 -0.09368490002929519 1102 36-37 0.11298438285363233 1102 38-39 0.09860044986677252 1102 40-41 0.09479235758199422 1102 42-43 0.17784434581702158 1102 44-45 -0.023194909124100604 1102 46-47 -0.08830370596012216 1102 48-49 -0.3121735930874152 1102 50-51 -0.37659271203919786 1102 52-53 -0.30184280198604796 1102 54-55 -0.24001733793441815 1102 56-57 -0.07748292490671105 1102 58-59 -0.2548704413961431 1102 60-61 -0.1004927074220916 1102 62-63 -0.0616879302259008 1102 64-65 -0.056903241757005674 1102 66-67 -0.1860120163317518 1102 68-69 -0.08715742414073446 1102 70-71 -0.04241115360162695 1102 72-73 -0.33256710616928586 1102 74-75 -0.353817412153866 1102 76-77 -0.06515646201067682 1102 78-79 -0.07526439543996233 1102 80-81 9.97405043150934E-4 1102 82-83 0.019602590728791114 1102 84-85 -0.16144449829570817 1102 86-87 -0.06139186135231611 1102 88-89 -0.29503104214380116 1102 90-91 -0.004024281475164315 1102 92-93 -0.006291462541859971 1102 94-95 -0.09027193185290372 1102 96-97 -0.14779693337068167 1102 98-99 -0.24120899482835867 1102 100-101 -0.08665118640829661 1103 1 0.20684897049373419 1103 2 0.30846138627959974 1103 3 0.14465205612560794 1103 4 0.2791974245444493 1103 5 0.35157462540102813 1103 6 0.29788740311690276 1103 7 0.124287598999274 1103 8 0.2291454896932379 1103 9 0.23259236943858497 1103 10-11 0.25927698216563755 1103 12-13 0.42255346219196355 1103 14-15 0.47408450508576294 1103 16-17 0.3271241385101078 1103 18-19 0.3155146442353285 1103 20-21 0.4380252837344898 1103 22-23 0.47712661502797715 1103 24-25 0.31628762913007336 1103 26-27 0.3693614815964068 1103 28-29 0.400331770210407 1103 30-31 0.38576797629802684 1103 32-33 0.6305771285950996 1103 34-35 0.35780570192168426 1103 36-37 0.5416972070268358 1103 38-39 0.46147458738758473 1103 40-41 0.36836480583323805 1103 42-43 0.4196763705612483 1103 44-45 0.5657439440759902 1103 46-47 0.5923363130739006 1103 48-49 0.8192658549259093 1103 50-51 0.6811553250771496 1103 52-53 0.9888745427676966 1103 54-55 0.7828532307070262 1103 56-57 0.9506564993140856 1103 58-59 0.9359361253419038 1103 60-61 0.8449641091375071 1103 62-63 0.8989104518821236 1103 64-65 0.8225495300726422 1103 66-67 0.8179411123858316 1103 68-69 1.1213139058616406 1103 70-71 1.056026866859952 1103 72-73 0.8470676785083242 1103 74-75 0.833492842426196 1103 76-77 0.7999910516700304 1103 78-79 0.6920970130777668 1103 80-81 0.69179207551662 1103 82-83 0.7359575752636474 1103 84-85 0.7681879071289899 1103 86-87 0.8370794686500638 1103 88-89 0.9069068593551286 1103 90-91 0.8513956566642626 1103 92-93 0.9447447021024402 1103 94-95 0.7314744397864033 1103 96-97 0.8336318080045224 1103 98-99 0.9058371626781856 1103 100-101 1.069287428866506 1104 1 0.26695444974263083 1104 2 0.32870642909314896 1104 3 0.27365728845835235 1104 4 0.26859225881921844 1104 5 0.30846577154468946 1104 6 0.1487932393404634 1104 7 0.21972758588592 1104 8 0.23711497512414326 1104 9 0.36794873266811123 1104 10-11 0.32132409657705097 1104 12-13 0.3939655675879621 1104 14-15 0.5739652099806349 1104 16-17 0.5410760718193259 1104 18-19 0.5297709947157685 1104 20-21 0.6886386713251937 1104 22-23 0.6483729821863093 1104 24-25 0.6690824129288302 1104 26-27 0.5285397894011865 1104 28-29 0.41616016218524265 1104 30-31 0.36997926262969827 1104 32-33 0.5080687311043306 1104 34-35 0.7052465161985921 1104 36-37 0.7147759313888002 1104 38-39 0.595770694230211 1104 40-41 0.5470908589995673 1104 42-43 0.6593118765312056 1104 44-45 0.5939766853135922 1104 46-47 0.7016409409954605 1104 48-49 0.8192372345081012 1104 50-51 0.8700435779567499 1104 52-53 0.9646031275400802 1104 54-55 0.755004263253646 1104 56-57 0.7765578473357593 1104 58-59 0.9768347023867676 1104 60-61 0.9136283942385006 1104 62-63 0.905404684867932 1104 64-65 1.0222732129480079 1104 66-67 0.9051429721926141 1104 68-69 0.9844835911151009 1104 70-71 0.9316626954600764 1104 72-73 0.7150358369925911 1104 74-75 0.6274212809742536 1104 76-77 0.6790834461842756 1104 78-79 0.7596795764785007 1104 80-81 0.8164476548068897 1104 82-83 0.8079730511041099 1104 84-85 1.0259329824657364 1104 86-87 1.072667136892587 1104 88-89 1.027016345462517 1104 90-91 0.841766187000605 1104 92-93 0.5652145452885726 1104 94-95 0.7388019172087965 1104 96-97 0.8292457289757458 1104 98-99 0.5139098117060001 1104 100-101 0.06003978159494139 1105 1 0.272168796307529 1105 2 0.179179064365794 1105 3 0.3690407910889846 1105 4 0.3162053258567141 1105 5 0.2734588337098032 1105 6 0.1736877343160188 1105 7 0.0789583865438388 1105 8 0.011058071259689939 1105 9 0.1511205866966705 1105 10-11 0.1453459526483769 1105 12-13 0.3308910399584235 1105 14-15 0.30566695419923207 1105 16-17 0.4932836954179507 1105 18-19 0.3334105965127918 1105 20-21 0.2256951832814238 1105 22-23 0.22890861027598675 1105 24-25 0.14454275378307102 1105 26-27 0.10642284943275371 1105 28-29 0.06809798950048673 1105 30-31 -0.2261403919463305 1105 32-33 0.23735017720029816 1105 34-35 -0.018787293830321516 1105 36-37 0.11591367578292022 1105 38-39 0.3616335202971044 1105 40-41 0.34561219984018976 1105 42-43 0.28190273795760135 1105 44-45 0.3204179316120275 1105 46-47 0.12303156455323006 1105 48-49 -0.0531504852971878 1105 50-51 0.09793821365611421 1105 52-53 0.2577898256602751 1105 54-55 0.2905105436208544 1105 56-57 0.5617422030855437 1105 58-59 0.5086745980393133 1105 60-61 0.37906935152352617 1105 62-63 0.3231266539722526 1105 64-65 0.5183783411958132 1105 66-67 0.5644771216231348 1105 68-69 0.7316256116970905 1105 70-71 0.5626621824991105 1105 72-73 0.318573754492121 1105 74-75 0.379977385134076 1105 76-77 0.389622145987115 1105 78-79 0.24966019290928543 1105 80-81 0.3415176762483547 1105 82-83 0.5106168428389353 1105 84-85 0.4538460787071976 1105 86-87 0.8801433538130361 1105 88-89 0.6286862679433689 1105 90-91 0.2987251304523326 1105 92-93 0.377802213949078 1105 94-95 0.7676843432266587 1105 96-97 0.962726797084553 1105 98-99 0.7010997086447297 1105 100-101 0.6026306732845192 1106 1 0.12762568311822875 1106 2 0.17391949619331015 1106 3 0.20749739625829022 1106 4 0.25393034205957576 1106 5 0.16835399863968092 1106 6 0.22530106443705478 1106 7 0.13455371876192146 1106 8 0.17215525111316765 1106 9 0.3072500195794916 1106 10-11 0.14004821019076275 1106 12-13 0.281857443194248 1106 14-15 0.4694371823287611 1106 16-17 0.4830326273862866 1106 18-19 0.25199248120269857 1106 20-21 0.3350840214597781 1106 22-23 0.31834162882699246 1106 24-25 0.4264886922087996 1106 26-27 0.24274746319382245 1106 28-29 0.2898345971129501 1106 30-31 0.29083189521931274 1106 32-33 0.23225518261256894 1106 34-35 0.5226835528922891 1106 36-37 0.40548513974130884 1106 38-39 0.4158605164728897 1106 40-41 0.4499467626682758 1106 42-43 0.36583102459388783 1106 44-45 0.5326058780390781 1106 46-47 0.7223169419357305 1106 48-49 0.5832063645268661 1106 50-51 0.5377170063985872 1106 52-53 0.5149555631365672 1106 54-55 0.5269747904337265 1106 56-57 0.49677381139362353 1106 58-59 0.3582721562423714 1106 60-61 0.36983880938080915 1106 62-63 0.538567897809223 1106 64-65 0.5191155290574088 1106 66-67 0.5952065728296105 1106 68-69 0.3650693394075475 1106 70-71 0.37455372681012733 1106 72-73 0.6628930815388543 1106 74-75 0.8249639986847654 1106 76-77 0.712535030571825 1106 78-79 0.7922711782808953 1106 80-81 0.5015756672290408 1106 82-83 0.5620155486458351 1106 84-85 0.6477426043382586 1106 86-87 0.44595751799979055 1106 88-89 0.37065015676627766 1106 90-91 0.5568537082311664 1106 92-93 0.607530820231375 1106 94-95 0.7075785071419496 1106 96-97 0.7120108171591362 1106 98-99 0.7280719618776672 1106 100-101 0.6978356437461066 1107 1 0.00970155592166222 1107 2 0.22897367784881695 1107 3 0.21361617072876982 1107 4 0.06886791577139917 1107 5 0.07864832832886748 1107 6 0.10368903540707208 1107 7 0.10838163146493685 1107 8 0.14192924465795187 1107 9 0.0298403130958107 1107 10-11 -0.009456002705611866 1107 12-13 0.11324638651332464 1107 14-15 0.1894237616935186 1107 16-17 0.13731447551497666 1107 18-19 0.027514795319504515 1107 20-21 0.2035673417914836 1107 22-23 0.2952270801929089 1107 24-25 0.20099151867592013 1107 26-27 0.16065975952736977 1107 28-29 0.024065350701953037 1107 30-31 0.08688352954212064 1107 32-33 -0.06427061051739713 1107 34-35 0.28141158723136783 1107 36-37 0.08877663372050648 1107 38-39 0.07059803768525796 1107 40-41 0.20708317621608785 1107 42-43 0.27416124616377147 1107 44-45 0.3247487061329437 1107 46-47 0.30695937258654027 1107 48-49 0.1036774547039272 1107 50-51 0.19914451093683283 1107 52-53 0.36357782216592227 1107 54-55 0.42949494911518116 1107 56-57 0.4173489761938498 1107 58-59 0.20036248488155906 1107 60-61 0.3499527923517647 1107 62-63 0.4961810581654831 1107 64-65 0.628720156403709 1107 66-67 0.6639827070211766 1107 68-69 0.4946690495514048 1107 70-71 0.44384468840048896 1107 72-73 0.2835651115300948 1107 74-75 0.5214524506533067 1107 76-77 0.5279560059525359 1107 78-79 0.4619239054796829 1107 80-81 0.4009310700514419 1107 82-83 0.4811192498573895 1107 84-85 0.4649982877739447 1107 86-87 0.6385214508744319 1107 88-89 0.29568130521033353 1107 90-91 0.25130965490355095 1107 92-93 0.26654963500073237 1107 94-95 0.24419964963360385 1107 96-97 0.23840011170695874 1107 98-99 0.11777411990102493 1107 100-101 0.37125021567220884 1108 1 -0.12100183401717857 1108 2 -0.028335153012175596 1108 3 -0.10942237446280956 1108 4 0.06527216638910716 1108 5 0.10329446020466548 1108 6 -0.08275303540855106 1108 7 -0.11560305783784486 1108 8 -0.12505834279326677 1108 9 -0.17236352151797973 1108 10-11 -0.06998384191407325 1108 12-13 -0.08395553974185788 1108 14-15 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-1.2293527875870538 2316 68-69 -0.8942675053857343 2316 70-71 -0.9665094486010517 2316 72-73 -1.6580259687393735 2316 74-75 -1.717623911753364 2316 76-77 -1.9061514271096591 2316 78-79 -1.9732378477800445 2316 80-81 -2.340165199373832 2316 82-83 -1.694962004762683 2316 84-85 -1.5648447729266728 2316 86-87 -1.7227861856457132 2316 88-89 -1.802276150279745 2316 90-91 -1.5751662885697932 2316 92-93 -1.8682133071465081 2316 94-95 -1.5857347994578923 2316 96-97 -1.7689738414837386 2316 98-99 -1.7460487934323154 2316 100-101 -1.7931874033667619 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1406.0 3 516.0 4 480.0 5 557.0 6 631.0 7 697.0 8 865.0 9 1066.0 10 1376.0 11 1812.0 12 2463.0 13 3117.0 14 3502.0 15 4558.0 16 6003.0 17 8328.0 18 7375.0 19 4304.0 20 4086.0 21 4250.0 22 4457.0 23 4846.0 24 5631.0 25 6293.0 26 7473.0 27 8782.0 28 10511.0 29 12811.0 30 15584.0 31 19631.0 32 25496.0 33 33933.0 34 46199.0 35 67115.0 36 102551.0 37 158071.0 38 158184.0 39 29999.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.01685495497472 20.15547498860977 15.19307887199966 21.634591184415846 2 14.75246515361317 21.38944200614014 41.259722209675814 22.598370630570873 3 18.538004903858564 26.55503935991741 30.592850690411666 24.31410504581236 4 12.052075643137032 18.039974393259644 39.066025121451325 30.841924842151997 5 12.951239824145745 38.32757696851537 34.49454813074157 14.22663507659731 6 29.25063191785455 38.11529693038963 18.456132378722117 14.177938773033697 7 26.59699404539131 34.26326236155599 21.87821831500319 17.26152527804951 8 25.260282178933668 35.354479538928715 20.614580966349234 18.770657315788387 9 26.030313043747643 17.55856339613484 21.437016919748654 34.97410664036886 10-11 23.634469847305336 28.00079764423905 28.121412294950254 20.243320213505356 12-13 25.113904148182858 25.738755293420574 28.545970545958927 20.60137001243764 14-15 22.362817193698845 26.662790607633557 27.405651732869412 23.56874046579819 16-17 20.732118105297392 30.169171719361827 27.771690452406578 21.327019722934203 18-19 20.564197902907008 28.373542995310615 30.91815090835527 20.144108193427112 20-21 21.796857254950545 27.417779119948793 30.725778975407298 20.059584649693367 22-23 21.319870646714808 27.57532273799508 30.644688824309746 20.46011779098037 24-25 21.056906792400728 27.794853729633058 30.780521543442386 20.36771793452383 26-27 20.877914898888235 27.879543319604018 30.719989057838614 20.522552723669136 28-29 20.62591052264161 28.038742294833195 30.823635070912697 20.511712111612496 30-31 20.923851331124254 27.780444499499346 30.727344048393256 20.568360120983144 32-33 20.73760993888843 27.96602733504005 30.55178482946569 20.744577896605833 34-35 21.097080713924417 28.038643378443943 30.399853416328593 20.464422491303047 36-37 21.141275174713282 27.734379032341312 30.39146614639786 20.73287964654754 38-39 20.89220021600474 28.139843067525348 30.434925669337265 20.533031047132646 40-41 21.001272284331034 28.073676359770005 30.40959555294045 20.515455802958513 42-43 20.92954237090209 28.13311509212777 30.112963695356093 20.824378841614045 44-45 21.01444740436409 28.32479659244358 29.833602908965158 20.827153094227167 46-47 21.229793131213032 28.424522312718715 29.49931869561178 20.846365860456473 48-49 21.19842783445293 28.719957366751743 29.1626773266992 20.91893747209613 50-51 21.140657278933862 28.864052501796817 29.024765704538037 20.970524514731288 52-53 21.388911470087653 29.04580886499153 28.691800186069077 20.87347947885174 54-55 21.201106108760477 28.866574017799422 28.80476509382956 21.127554779610538 56-57 21.256663877349173 29.197508840545023 28.53762126918032 21.008206012925488 58-59 21.413612497887037 28.79439313839472 28.727359063923025 21.06463529979522 60-61 21.34946581010467 28.846705984901128 28.96471230394918 20.839115901045023 62-63 21.220418768109724 28.862068987765234 29.0257541161616 20.89175812796345 64-65 21.15585738627529 28.96695310619898 28.841529624523126 21.0356598830026 66-67 21.129151629615244 29.101816189750433 28.55251348091959 21.216518699714733 68-69 20.98660594160095 28.984711402146328 28.854558606506863 21.174124049745856 70-71 21.099330029410037 28.963949631842095 28.814414726058118 21.12230561268975 72-73 21.02555300090793 28.967574616969554 28.662608627194885 21.344263754927628 74-75 20.989180754830457 29.241194440536745 28.49431418530426 21.275310619328543 76-77 21.10011275165013 29.1116757657528 28.53855919268011 21.249652289916956 78-79 21.04920804442308 29.20952863397746 28.308289963185118 21.432973358414348 80-81 21.042782215766962 29.349858791253126 28.337287178245784 21.270071814734123 82-83 21.110241922679236 29.32465349717105 28.38367096140416 21.181433618745555 84-85 21.018266484427738 29.22327753879353 28.25039009714677 21.50806587963196 86-87 21.037980564481785 29.092170299534114 28.38443868003665 21.48541045594745 88-89 21.1392296429938 28.93971862540321 28.203986581052803 21.717065150550184 90-91 20.940663226947265 28.8606010151367 28.661123103878882 21.537612654037154 92-93 21.143039331669478 28.897330795598076 28.44244884998383 21.517181022748616 94-95 20.888701907255676 28.886425434549473 28.491075426870893 21.733797231323955 96-97 20.86882733476374 28.83701421265749 28.56450546082243 21.729652991756335 98-99 20.8485028413583 29.179733257606806 28.105083465908542 21.866680435126355 100-101 20.708504728937303 29.192579057130004 28.10769571371679 21.991220500215906 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 424.0 1 296.0 2 196.5 3 232.5 4 236.5 5 266.0 6 420.5 7 758.0 8 1229.5 9 1537.0 10 1401.5 11 1207.0 12 1274.0 13 1375.5 14 1466.5 15 1536.5 16 1526.0 17 1577.5 18 1699.0 19 1768.5 20 1978.5 21 2338.5 22 2744.5 23 3270.0 24 3843.5 25 4645.0 26 5937.0 27 7383.5 28 8489.0 29 9867.0 30 11719.0 31 13469.5 32 15124.5 33 17224.0 34 19941.0 35 22133.0 36 24108.5 37 26018.5 38 26946.5 39 27003.5 40 26969.0 41 27636.5 42 27963.0 43 28253.5 44 29053.0 45 29199.0 46 28241.0 47 27859.0 48 29335.0 49 28848.0 50 26363.0 51 24175.0 52 21948.5 53 20588.0 54 19329.0 55 17430.0 56 15473.0 57 13655.5 58 12109.0 59 10548.5 60 8712.0 61 6846.5 62 5339.0 63 4087.0 64 2998.5 65 2176.0 66 1539.5 67 1175.0 68 866.5 69 565.0 70 370.5 71 259.5 72 195.5 73 149.5 74 100.0 75 69.5 76 46.5 77 24.5 78 17.5 79 17.5 80 13.0 81 9.5 82 5.5 83 4.5 84 3.0 85 2.0 86 2.0 87 1.5 88 1.5 89 1.5 90 2.0 91 1.5 92 1.0 93 0.5 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02541987963622475 2 0.01070989852693733 3 0.010838933448948623 4 0.02425856533812311 5 0.06180772764340941 6 0.04619450208004294 7 0.015742260485377763 8 0.05677536568496898 9 0.034581359099026564 10-11 0.026129571707286862 12-13 0.041226657582608156 14-15 0.018322958925603625 16-17 0.019355238301693972 18-19 0.019290720840688325 20-21 0.015484190641355176 22-23 0.006709815944587243 24-25 0.001935523830169397 26-27 0.0 28-29 0.0021935936741919832 30-31 0.0 32-33 0.001032279376090345 34-35 0.0 36-37 0.0 38-39 1.2903492201129312E-4 40-41 0.0 42-43 6.451746100564656E-5 44-45 6.451746100564656E-5 46-47 0.0 48-49 2.5806984402258624E-4 50-51 1.2903492201129312E-4 52-53 3.8710476603387944E-4 54-55 0.001935523830169397 56-57 4.51622227039526E-4 58-59 6.451746100564657E-4 60-61 0.0032903905112879753 62-63 0.0026452159012315094 64-65 0.0012258317591072848 66-67 0.011935730286044615 68-69 0.01619388271241729 70-71 0.032065178119806345 72-73 0.018516511308620566 74-75 0.020774622443818197 76-77 0.03516201624807738 78-79 0.020839139904823844 80-81 0.05438821962776006 82-83 0.04064600043355734 84-85 0.022839181195998884 86-87 0.014709981109287418 88-89 0.0141293239602366 90-91 0.027290886005388496 92-93 0.035807190858133844 94-95 0.013032527123140607 96-97 0.018903616074654444 98-99 0.03438780671600962 100-101 0.043678321100822724 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 774984.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.38842817619451 #Duplication Level Percentage of deduplicated Percentage of total 1 85.4822718084775 59.31480477720478 2 9.209374042478306 12.780479785884319 3 2.322529578032091 4.834700304371011 4 0.9227416202230656 2.5611036256013415 5 0.498737990220874 1.7303322606590354 6 0.30368213928790894 1.2643215786223299 7 0.19757486825429738 0.9596586688699085 8 0.15791938920863163 0.8766222556585314 9 0.11634639035423197 0.7265783835588704 >10 0.7254657087072355 9.340658501926017 >50 0.04528187148855268 2.143289648833278 >100 0.01677024118342015 1.7863484296060834 >500 7.453440479360411E-4 0.35349425198778234 >1k 5.590080359520308E-4 1.3276075272167336 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4717 0.6086577271272697 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3459 0.446331795237063 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2092 0.26994105684762526 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 788 0.101679518544899 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 776 0.10013109948076347 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.2903492201129315E-4 0.0 0.0 0.0 0.0 7 2.580698440225863E-4 0.0 0.0 0.0 0.0 8 2.580698440225863E-4 0.0 0.0 0.0 0.0 9 2.580698440225863E-4 0.0 0.0 0.0 0.0 10-11 2.580698440225863E-4 0.0 0.0 0.0 0.0 12-13 2.580698440225863E-4 0.0 0.0 0.0 0.0 14-15 2.580698440225863E-4 0.0 0.0 1.9355238301693972E-4 0.0 16-17 3.2258730502823286E-4 0.0 0.0 3.2258730502823286E-4 0.0 18-19 4.51622227039526E-4 0.0 0.0 3.8710476603387944E-4 0.0 20-21 5.161396880451726E-4 0.0 0.0 4.51622227039526E-4 0.0 22-23 5.161396880451726E-4 0.0 0.0 6.451746100564657E-4 0.0 24-25 6.451746100564657E-4 0.0 0.0 9.032444540790519E-4 0.0 26-27 6.451746100564657E-4 0.0 0.0 0.0011613142981016383 0.0 28-29 7.096920710621123E-4 0.0 0.0 0.0026452159012315094 0.0 30-31 7.742095320677589E-4 0.0 0.0 0.005161396880451726 0.0 32-33 7.742095320677589E-4 0.0 0.0 0.008516304852745347 0.0 34-35 8.387269930734053E-4 0.0 0.0 0.011290555675988148 0.0 36-37 9.032444540790519E-4 0.0 0.0 0.014968050953310004 0.0 38-39 9.032444540790519E-4 0.0 0.0 0.019161685918677028 0.0 40-41 9.032444540790519E-4 0.0 0.0 0.02212948912493677 0.0 42-43 0.0010967968370959916 0.0 0.0 0.03309745749589669 0.0 44-45 0.0011613142981016383 0.0 0.0 0.04522674016495824 0.0 46-47 0.0012903492201129315 0.0 0.0 0.05374304501770359 0.0 48-49 0.0012903492201129315 0.0 0.0 0.06542070545972561 0.0 50-51 0.0012903492201129315 0.0 0.0 0.08122748340610902 0.0 52-53 0.0012903492201129315 0.0 0.0 0.11987344254849132 0.0 54-55 0.0012903492201129315 0.0 0.0 0.15193862066829766 0.0 56-57 0.0014193841421242246 0.0 0.0 0.21587542452489342 0.0 58-59 0.0014193841421242246 0.0 0.0 0.2805864379135569 0.0 60-61 0.0014193841421242246 0.0 0.0 0.3467813529053503 0.0 62-63 0.0014193841421242246 0.0 0.0 0.45258998895461067 0.0 64-65 0.0014193841421242246 0.0 0.0 0.49923611326169315 0.0 66-67 0.0018064889081581038 0.0 0.0 0.625045162222704 0.0 68-69 0.0018064889081581038 0.0 0.0 0.795177706894594 0.0 70-71 0.0018710063691637505 0.0 0.0 0.9029218667740238 0.0 72-73 0.001935523830169397 0.0 0.0 1.1033131006575618 0.0 74-75 0.001935523830169397 0.0 0.0 1.3020268805549535 0.0 76-77 0.001935523830169397 0.0 0.0 1.4154485770028802 0.0 78-79 0.001935523830169397 0.0 0.0 1.5245476035634284 0.0 80-81 0.0023226285962032766 0.0 0.0 1.7594556790849876 0.0 82-83 0.0023226285962032766 0.0 0.0 1.9384916333756568 0.0 84-85 0.0023226285962032766 0.0 0.0 2.2577885478926016 0.0 86-87 0.0023226285962032766 0.0 0.0 2.701475127228433 0.0 88-89 0.0023226285962032766 0.0 0.0 3.295551908168427 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3130 0.0 35.530468 1 GTATCAA 4820 0.0 33.130016 1 ATCAACG 5885 0.0 27.218798 3 TCAACGC 5925 0.0 26.95482 4 TATCAAC 6000 0.0 26.617886 2 CAACGCA 6005 0.0 26.516567 5 AACGCAG 6235 0.0 25.685904 6 GAGTACT 3445 0.0 23.17135 12-13 ACGCAGA 6860 0.0 22.86079 7 CGCAGAG 6910 0.0 22.626596 8 AGTACTT 3565 0.0 22.191465 12-13 GTACATG 3830 0.0 21.839533 1 GCAGAGT 7085 0.0 21.796602 9 TACATGG 3860 0.0 21.549454 2 GTACTTT 3785 0.0 20.960989 14-15 AGAGTAC 5735 0.0 20.547716 10-11 ACATGGG 3840 0.0 20.54766 3 TACCTGG 535 0.0 20.434292 2 ATGGGCG 140 4.827838E-4 20.37083 5 TATACAG 615 0.0 20.094803 5 >>END_MODULE