FastQCFastQC Report
Thu 26 May 2016
SRR936829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936829_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774984
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66600.8593725805952123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51320.6622072197619564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34010.43884776976040796No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13000.16774539861468107No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9670.12477676958492046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30350.039.7585031
GTATCAA51850.038.7566831
TATCAAC64300.031.0208132
ATCAACG64150.030.9452843
TCAACGC64850.030.684494
CAACGCA65650.030.3242685
AACGCAG67700.029.6165736
ACGCAGA75700.026.2356267
CGCAGAG75750.026.2183078
GCAGAGT79300.024.5053639
GTACATG34900.023.5491711
GAGTACT46950.022.86060312-13
TACATGG34700.022.4454982
AGTACTT48550.021.61811812-13
AGAGTAC67500.021.39837810-11
GTACTTT50300.021.33945714-15
ACATGGG35600.020.8108333
TACACGA1150.003716370820.6577597
CTATACA3503.6379788E-1220.3534534
CATGGGG12950.019.8033584
Produced by FastQC (version 0.11.5)