FastQCFastQC Report
Thu 26 May 2016
SRR936828_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936828_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824925
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49560.6007818892626603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34320.41603782162014724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23250.28184380398218023No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8810.10679758765948419No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA702.0432216E-433.9556776
GGTATCA33400.032.4569661
GTATCAA51350.031.574281
GTATAGG1703.59978E-927.9685921
TAGGACG700.00834522727.172794
ATCAACG63700.025.5304783
TATCAAC63500.025.4611192
TCAACGC63850.025.3960254
CAACGCA64550.025.1190975
AACGCAG66450.024.5380236
ACGCAGT1401.770222E-523.7646457
GAGTACT33800.022.92232112-13
GTACATG41750.022.4351651
TACATGG40950.021.5988852
ACGCAGA73900.021.4814387
CGCAGAG74000.021.3239528
AGTACTT35550.020.92485212-13
GTACTTT37400.020.71715214-15
CTACTCG1150.003698701320.6749484
TACCTGG5350.020.4430792
Produced by FastQC (version 0.11.5)