##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936828_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 824925 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.818741097675545 33.0 31.0 34.0 30.0 34.0 2 31.90357911325272 33.0 31.0 34.0 30.0 34.0 3 31.917973149074157 33.0 31.0 34.0 30.0 34.0 4 35.521503166954574 37.0 35.0 37.0 33.0 37.0 5 35.5296881534685 37.0 35.0 37.0 33.0 37.0 6 35.60249113555778 37.0 35.0 37.0 33.0 37.0 7 35.555130466406034 37.0 35.0 37.0 33.0 37.0 8 35.52283540927963 37.0 35.0 37.0 33.0 37.0 9 37.151995635966905 39.0 37.0 39.0 33.0 39.0 10-11 37.02800254568597 39.0 37.0 39.0 33.0 39.0 12-13 36.94930630057278 39.0 37.0 39.0 33.0 39.0 14-15 38.09655605055005 40.0 38.0 41.0 33.0 41.0 16-17 38.051215565051365 40.0 38.0 41.0 33.0 41.0 18-19 38.03540200624299 40.0 38.0 41.0 33.0 41.0 20-21 37.995391096160255 40.0 38.0 41.0 33.0 41.0 22-23 37.88923114222505 40.0 38.0 41.0 33.0 41.0 24-25 37.85425220474588 40.0 37.5 41.0 32.5 41.0 26-27 37.87591902294148 40.0 38.0 41.0 33.0 41.0 28-29 37.79334424341607 40.0 38.0 41.0 33.0 41.0 30-31 37.62784131890778 40.0 37.0 41.0 32.5 41.0 32-33 37.440043034215236 40.0 37.0 41.0 32.0 41.0 34-35 37.27959996363306 40.0 36.0 41.0 31.5 41.0 36-37 37.10919780586114 40.0 36.0 41.0 31.0 41.0 38-39 36.95902354759524 39.0 36.0 41.0 31.0 41.0 40-41 36.78393066036306 39.0 36.0 41.0 30.5 41.0 42-43 36.55878049519653 39.0 35.0 41.0 30.0 41.0 44-45 36.30486953359396 39.0 35.0 41.0 30.0 41.0 46-47 35.92667333393945 39.0 35.0 40.5 28.5 41.0 48-49 35.6548201351638 39.0 35.0 40.5 27.5 41.0 50-51 34.96398460466103 38.5 34.0 40.0 26.5 40.5 52-53 35.140244870745825 38.5 35.0 40.0 27.0 40.5 54-55 35.532862381428615 39.0 35.0 40.5 27.0 41.0 56-57 35.430432463557295 39.0 35.0 41.0 27.0 41.0 58-59 35.23459708458345 39.0 35.0 41.0 26.0 41.0 60-61 34.97662757220354 38.5 35.0 40.0 26.0 41.0 62-63 34.68743461526805 38.0 34.0 40.0 26.0 41.0 64-65 34.37707367336425 38.0 34.0 40.0 25.5 41.0 66-67 34.027997090644604 37.0 34.0 40.0 24.5 41.0 68-69 33.63669485104707 37.0 34.0 39.5 23.0 41.0 70-71 33.23955632330212 36.0 33.0 39.0 22.0 41.0 72-73 32.86136436645755 36.0 33.0 39.0 22.0 40.0 74-75 32.46890626420584 35.0 32.5 38.0 20.5 40.0 76-77 32.00291481043731 35.0 32.0 37.0 19.5 39.0 78-79 31.545670212443554 35.0 31.5 37.0 18.0 39.0 80-81 31.087896475437162 35.0 31.0 36.0 14.0 39.0 82-83 30.68742128072249 35.0 31.0 36.0 9.5 37.0 84-85 30.310271842894807 34.5 31.0 35.5 7.0 37.0 86-87 29.952590235475952 34.0 30.5 35.0 4.5 36.5 88-89 29.59898415007425 34.0 30.0 35.0 2.0 36.0 90-91 29.231676212989058 34.0 30.0 35.0 2.0 36.0 92-93 28.851681668030427 34.0 29.0 35.0 2.0 35.5 94-95 28.477590083947025 34.0 29.0 35.0 2.0 35.0 96-97 28.02240264266448 34.0 29.0 35.0 2.0 35.0 98-99 27.49900597023972 34.0 28.0 35.0 2.0 35.0 100-101 25.357914355850532 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22451763439897476 1101 2 0.1976943698869178 1101 3 0.22290473568065394 1101 4 0.35996608160573373 1101 5 0.14742459781831485 1101 6 0.11012767410889523 1101 7 0.1938513799066257 1101 8 0.3247715837896834 1101 9 0.2621417919905298 1101 10-11 0.20409447783854517 1101 12-13 0.3128180257037272 1101 14-15 0.5910357842449443 1101 16-17 0.5179880135061197 1101 18-19 0.337738992242123 1101 20-21 0.4441399061436542 1101 22-23 0.5484981574970575 1101 24-25 0.3902739847143337 1101 26-27 0.33871432016067615 1101 28-29 0.5384448750054318 1101 30-31 0.4334113512684965 1101 32-33 0.3652047886109884 1101 34-35 0.38015581103931595 1101 36-37 0.4492443967321904 1101 38-39 0.4604430818646108 1101 40-41 0.43558051817502985 1101 42-43 0.4459679748907064 1101 44-45 0.5558557265955955 1101 46-47 0.5349651609243224 1101 48-49 0.7545134586015436 1101 50-51 0.9363246439654702 1101 52-53 0.9337556610337785 1101 54-55 0.9077508288864351 1101 56-57 0.8018031523424511 1101 58-59 0.7587795209586758 1101 60-61 0.9547330837963202 1101 62-63 0.9779361007326699 1101 64-65 0.841622911502995 1101 66-67 0.8042083309258246 1101 68-69 0.8110628122254653 1101 70-71 0.8200727821254219 1101 72-73 0.7746045063878526 1101 74-75 0.7006584791383332 1101 76-77 0.6632044526221499 1101 78-79 0.6860319924380818 1101 80-81 0.7055766675690549 1101 82-83 0.6722807264734527 1101 84-85 0.5213535452530351 1101 86-87 0.7676904570571494 1101 88-89 1.0093517481596166 1101 90-91 0.9793525789010928 1101 92-93 0.8802421502320499 1101 94-95 0.914221525776437 1101 96-97 0.8901429348387673 1101 98-99 0.8199289421017824 1101 100-101 1.0330609280938354 1102 1 0.10770906886749643 1102 2 0.16004202824851177 1102 3 0.22976010940908864 1102 4 0.1723862251017323 1102 5 0.22073593989900786 1102 6 0.2504509086658757 1102 7 0.16402190425671392 1102 8 0.21208791383047298 1102 9 0.3040326711492085 1102 10-11 0.2957743515424127 1102 12-13 0.27786544818148684 1102 14-15 0.21237467067670224 1102 16-17 0.42853337200039476 1102 18-19 0.25883155207307595 1102 20-21 0.10246924665178625 1102 22-23 0.1537966365377983 1102 24-25 0.250624923335387 1102 26-27 0.30553087134255463 1102 28-29 0.07119986296552838 1102 30-31 0.2673236442558604 1102 32-33 0.1938358024200184 1102 34-35 0.1809122978445643 1102 36-37 0.16106530930774454 1102 38-39 0.20210114860007877 1102 40-41 0.44128257970139373 1102 42-43 0.22290879507842476 1102 44-45 0.350403370214444 1102 46-47 0.5600109248439296 1102 48-49 0.69065282662946 1102 50-51 0.6116065988526884 1102 52-53 0.853066806544625 1102 54-55 0.7243311891006954 1102 56-57 0.7709324707364758 1102 58-59 0.5831520331214293 1102 60-61 0.5146838184761648 1102 62-63 0.45936792784949176 1102 64-65 0.5497919247223209 1102 66-67 0.3185901801567468 1102 68-69 0.505278437071901 1102 70-71 0.49619323030469076 1102 72-73 0.5639261561003721 1102 74-75 0.681540524939102 1102 76-77 0.6279708416338927 1102 78-79 0.5210991333295283 1102 80-81 0.5796782818989961 1102 82-83 0.7087029708097816 1102 84-85 0.6831305367513849 1102 86-87 0.7129810733036486 1102 88-89 0.48085372614379196 1102 90-91 0.24749996943625163 1102 92-93 0.573273748836403 1102 94-95 0.6361427504066413 1102 96-97 0.4853549231428609 1102 98-99 0.5605104817351787 1102 100-101 0.642811407232859 1103 1 0.20167828301147495 1103 2 0.3478541746981776 1103 3 0.24628439847856853 1103 4 0.26505535736953334 1103 5 0.1387427984519647 1103 6 0.15916466603705715 1103 7 0.23317570207949245 1103 8 0.28590896418483425 1103 9 0.15722485865082803 1103 10-11 0.2686398003139061 1103 12-13 0.20858071058376026 1103 14-15 0.3637089625098966 1103 16-17 0.4121834967813385 1103 18-19 0.419662341655453 1103 20-21 0.28013847725380714 1103 22-23 0.24647353203336309 1103 24-25 0.34229622206128596 1103 26-27 0.4001884250149246 1103 28-29 0.3951852349742282 1103 30-31 0.4160174035523241 1103 32-33 0.28075600575448334 1103 34-35 0.2516616977470605 1103 36-37 0.14755211751357677 1103 38-39 0.4216043434270773 1103 40-41 0.3439280807038827 1103 42-43 0.2509190383986919 1103 44-45 0.11740147495177666 1103 46-47 0.043078289994078034 1103 48-49 0.04974040929489831 1103 50-51 0.0895491985817003 1103 52-53 0.12706475498229253 1103 54-55 0.18051604915039832 1103 56-57 0.16015897377500465 1103 58-59 0.2527868405364515 1103 60-61 0.26255436510055574 1103 62-63 0.3974660198551234 1103 64-65 0.42139745338260326 1103 66-67 0.43296115686395353 1103 68-69 0.39351901018442703 1103 70-71 0.4101704560997277 1103 72-73 0.21314781488477763 1103 74-75 0.22327046191204403 1103 76-77 0.15820085054607702 1103 78-79 0.4399599469364972 1103 80-81 0.18648218173058595 1103 82-83 0.2900243342810036 1103 84-85 0.258382091208361 1103 86-87 0.2592768232987339 1103 88-89 0.14421118758901486 1103 90-91 0.24235139678330953 1103 92-93 0.3519270461897648 1103 94-95 0.5183533657407757 1103 96-97 0.6305272823256729 1103 98-99 0.8075291434996288 1103 100-101 1.1276094546258122 1104 1 0.12577608334952473 1104 2 0.028621128708554266 1104 3 0.04836085195854167 1104 4 0.09369602858274817 1104 5 0.23003267662436855 1104 6 0.07589671918690755 1104 7 0.05614407357604989 1104 8 0.11743984284001385 1104 9 0.08539249924604064 1104 10-11 0.2453741656297055 1104 12-13 0.17260779717972952 1104 14-15 0.08780654875850047 1104 16-17 0.253111296188095 1104 18-19 0.21525991345301776 1104 20-21 0.4430562594156626 1104 22-23 0.19394133565768357 1104 24-25 0.14852547545648065 1104 26-27 0.2219070494046349 1104 28-29 0.2406453078288635 1104 30-31 0.35339142529739576 1104 32-33 0.04087414030755809 1104 34-35 0.2723443433359094 1104 36-37 0.20364515650248194 1104 38-39 0.2156310339575782 1104 40-41 0.28424636747454457 1104 42-43 0.29069203936530386 1104 44-45 0.20974520839329358 1104 46-47 0.15835742515837126 1104 48-49 0.29324734418707976 1104 50-51 0.41903605830708557 1104 52-53 0.5319191259928715 1104 54-55 0.6923857029356668 1104 56-57 0.8052409646783474 1104 58-59 0.7432363106115716 1104 60-61 0.8948461176260096 1104 62-63 1.0000341297343809 1104 64-65 0.44368951633774145 1104 66-67 0.4394587713723723 1104 68-69 0.6401912574491675 1104 70-71 0.6638434280572199 1104 72-73 0.8145448555264423 1104 74-75 0.8302921673699402 1104 76-77 0.7301793259937703 1104 78-79 0.775126599508944 1104 80-81 0.6806284412134112 1104 82-83 0.585952674263595 1104 84-85 0.5394033834736298 1104 86-87 0.6936557337761613 1104 88-89 0.39159635268944015 1104 90-91 0.4001246096984694 1104 92-93 0.4108193046085624 1104 94-95 0.6096887379192104 1104 96-97 0.5068681512504831 1104 98-99 0.3634193327439874 1104 100-101 0.3279388312294742 1105 1 0.09585229198505019 1105 2 0.044674488078726426 1105 3 0.19012272389750606 1105 4 0.14115471104918953 1105 5 0.0790848233917103 1105 6 0.11363761951724882 1105 7 0.14929522978861343 1105 8 0.07714532935298735 1105 9 0.2221585928841705 1105 10-11 0.27407324024778035 1105 12-13 0.2540599405551731 1105 14-15 0.20104560353399137 1105 16-17 0.29145183528151364 1105 18-19 0.27072347055862167 1105 20-21 0.19027581321174125 1105 22-23 0.3227158799625087 1105 24-25 0.20753780378716868 1105 26-27 0.2696594942954107 1105 28-29 0.3433264805042384 1105 30-31 0.43548500044061456 1105 32-33 0.36393346093500867 1105 34-35 0.2451546624527552 1105 36-37 0.2340989316221922 1105 38-39 0.48699119638094146 1105 40-41 0.4775844413863837 1105 42-43 0.5409116040639006 1105 44-45 0.5408790586677341 1105 46-47 0.9458387848725849 1105 48-49 1.0267600038333313 1105 50-51 0.8619401512353448 1105 52-53 0.9123547908105536 1105 54-55 0.7577414825447946 1105 56-57 0.8935538234773546 1105 58-59 0.7565313316716029 1105 60-61 0.5799031196502469 1105 62-63 0.6472642226359966 1105 64-65 0.919372650255859 1105 66-67 0.6746607163824621 1105 68-69 0.5782201055249274 1105 70-71 0.5536479942761119 1105 72-73 0.597163084302565 1105 74-75 0.7322451726503871 1105 76-77 0.7147934433073395 1105 78-79 0.6406397388662057 1105 80-81 0.5877637790765426 1105 82-83 0.5456248475119558 1105 84-85 0.7260091494546081 1105 86-87 0.661424241717441 1105 88-89 0.7993906874962278 1105 90-91 0.8232951360601639 1105 92-93 0.9196364172086113 1105 94-95 0.8729016872091542 1105 96-97 0.5406150940013355 1105 98-99 0.7980478050461315 1105 100-101 0.6243972072138853 1106 1 0.19450998913903206 1106 2 0.18563488248339155 1106 3 0.14710744428703393 1106 4 0.1801295445444282 1106 5 0.12852078246955045 1106 6 -0.038326891203467994 1106 7 0.2826820191959811 1106 8 0.23055285654343294 1106 9 0.23979760966928154 1106 10-11 0.37693651220580904 1106 12-13 0.4037238069764797 1106 14-15 0.29001277565311767 1106 16-17 0.35537151430704483 1106 18-19 0.4477124158623411 1106 20-21 0.2279110216234841 1106 22-23 0.133146657569867 1106 24-25 0.3106495126013229 1106 26-27 0.21533438714771336 1106 28-29 0.24304204261865436 1106 30-31 0.4065755787331753 1106 32-33 0.27341616184529727 1106 34-35 0.4096310185535188 1106 36-37 0.1612228807863616 1106 38-39 0.14888053635711174 1106 40-41 0.18036990946670528 1106 42-43 0.36558807828374285 1106 44-45 0.31452035452628024 1106 46-47 0.32684571283927966 1106 48-49 0.16743030729820418 1106 50-51 0.29262106161509394 1106 52-53 0.22353831721552098 1106 54-55 0.07819006727292077 1106 56-57 0.10953396638060298 1106 58-59 0.24623310330904502 1106 60-61 0.2718384427594813 1106 62-63 -0.07997219255405952 1106 64-65 -0.11469443959646952 1106 66-67 -0.057544435930154236 1106 68-69 0.025844558257212213 1106 70-71 0.17694259427046433 1106 72-73 -0.03949280684935985 1106 74-75 0.022273631315407272 1106 76-77 0.2361575252885224 1106 78-79 0.2531965111507688 1106 80-81 0.2429155710203439 1106 82-83 0.13315792903643242 1106 84-85 0.10443203658840616 1106 86-87 0.4453198803861156 1106 88-89 0.4690730602318425 1106 90-91 0.37478999698759097 1106 92-93 0.5605246607897918 1106 94-95 0.8212588055201415 1106 96-97 0.9009236615025209 1106 98-99 0.7961785780417827 1106 100-101 0.7714019066871067 1107 1 0.3681675616187654 1107 2 0.34718404202942565 1107 3 0.3527654135762681 1107 4 0.1831838330440121 1107 5 0.3531283130744356 1107 6 0.32154116963810964 1107 7 0.15669881873659364 1107 8 0.3248408982694784 1107 9 0.3092106559262078 1107 10-11 0.41752459151428667 1107 12-13 0.4644261216349648 1107 14-15 0.46168630063781535 1107 16-17 0.42914851118408137 1107 18-19 0.39205988362633803 1107 20-21 0.6196030135117852 1107 22-23 0.4638673437450649 1107 24-25 0.5900014921656336 1107 26-27 0.6454482218727478 1107 28-29 0.5530160783048004 1107 30-31 0.5769594728460916 1107 32-33 0.27763893985518706 1107 34-35 0.33008692977501397 1107 36-37 0.3658525793065266 1107 38-39 0.6137667111707756 1107 40-41 0.5017216944417484 1107 42-43 0.8316532351665771 1107 44-45 0.7517557751157256 1107 46-47 0.7902915454810611 1107 48-49 0.761930107939591 1107 50-51 0.7847468731191398 1107 52-53 0.9282061704952085 1107 54-55 1.0406028598006927 1107 56-57 0.9125113730397558 1107 58-59 0.7997508767498971 1107 60-61 0.8249460525354948 1107 62-63 0.8594494957558965 1107 64-65 0.7628621677586267 1107 66-67 0.848957325865868 1107 68-69 0.44412278657911486 1107 70-71 0.6738625513082042 1107 72-73 0.784540303850946 1107 74-75 0.8535857162631615 1107 76-77 0.8271137026394761 1107 78-79 0.7729501840233013 1107 80-81 0.770292564844997 1107 82-83 0.9750875296896417 1107 84-85 0.9242786163003736 1107 86-87 0.8915922557679039 1107 88-89 0.8221095113813526 1107 90-91 1.001894947376865 1107 92-93 0.847837630927966 1107 94-95 0.8734039614872593 1107 96-97 0.7918744971871412 1107 98-99 1.138786379545465 1107 100-101 1.4098674367234878 1108 1 -0.26902041357455886 1108 2 -0.18316692831308856 1108 3 -0.07604362778383944 1108 4 -0.0071047955457856915 1108 5 -0.2547254474768863 1108 6 -0.2088712033577238 1108 7 -0.26806125942536596 1108 8 -0.11451314581762517 1108 9 -0.2753769168991482 1108 10-11 -0.24294582504852968 1108 12-13 -0.1719293058177982 1108 14-15 -0.08629987292942332 1108 16-17 0.028432989676893783 1108 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72-73 -2.037613634432624 2316 74-75 -2.284932354275824 2316 76-77 -2.357088398055126 2316 78-79 -2.7191410832416096 2316 80-81 -2.6360699961880947 2316 82-83 -3.1360742261661265 2316 84-85 -3.1463233693688935 2316 86-87 -3.3830314661316763 2316 88-89 -2.968492503933618 2316 90-91 -3.3376298912763893 2316 92-93 -3.6550276263819192 2316 94-95 -4.154996623126028 2316 96-97 -4.380386313860033 2316 98-99 -4.5589756662452885 2316 100-101 -4.602479999071331 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1398.0 3 541.0 4 491.0 5 527.0 6 603.0 7 671.0 8 853.0 9 1158.0 10 1497.0 11 1860.0 12 2477.0 13 3271.0 14 3694.0 15 4819.0 16 6322.0 17 8576.0 18 7744.0 19 4541.0 20 4315.0 21 4371.0 22 4694.0 23 5256.0 24 6021.0 25 6857.0 26 8063.0 27 9471.0 28 11455.0 29 13887.0 30 16830.0 31 21263.0 32 27165.0 33 35712.0 34 48743.0 35 70962.0 36 106822.0 37 166957.0 38 170871.0 39 34147.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.91267104805172 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20.56817947464386 28.455133158724134 30.764560235928656 20.212127130703358 20-21 21.7134324266459 27.526991676719742 30.66670548397603 20.092870412658325 22-23 21.31177361936518 27.629371320535512 30.59659092631703 20.46226413378228 24-25 21.001144469930992 27.831896279987777 30.701254552420114 20.46570469766111 26-27 20.916834062114724 27.94089657688937 30.56256993331256 20.579699427683348 28-29 20.71073922286159 28.032592217220024 30.750752097781504 20.505916462136888 30-31 20.939223371355347 27.78718663357874 30.678965125540536 20.59462486952537 32-33 20.65543860074497 28.007710329234698 30.55660454557853 20.780246524441804 34-35 21.083437593955793 28.014768638404764 30.379605652380537 20.522188115258906 36-37 21.11759564245959 27.683123017109384 30.38215765169985 20.817123688731183 38-39 20.842738718992535 28.129174839552807 30.381072686760046 20.64701375469461 40-41 21.013989807850027 27.973250910316477 30.392416565183627 20.620342716649866 42-43 20.910609022442454 28.082496097952752 30.043369550393233 20.96352532921156 44-45 21.02937539701397 28.32673461767858 29.79283750430351 20.851052481003943 46-47 21.2345884048677 28.466195865834493 29.420728288599978 20.878487440697825 48-49 21.21820993098148 28.720117683639756 29.081726972258643 20.979945413120127 50-51 21.05186912885515 28.823813224100288 29.029776834367194 21.09454081267737 52-53 21.336002056096522 28.991324630484538 28.701761744966444 20.970911568452497 54-55 21.139823000266126 28.875301814310923 28.825411143381423 21.159464042041527 56-57 21.168724339704877 29.215320071359336 28.577923341450063 21.03803224748572 58-59 21.347923676494922 28.875774579076925 28.686717578906595 21.089584165521554 60-61 21.287013487662776 28.76753844699128 29.028357307898396 20.91709075744755 62-63 21.212172644827934 28.949947838969415 29.00019943032032 20.837680085882333 64-65 21.11246009372028 28.893267862890298 28.89884428625721 21.095427757132214 66-67 21.08064816336132 29.02639082477921 28.588291856609587 21.30466915524988 68-69 21.001954592564786 29.05853892538041 28.81341130679947 21.12609517525533 70-71 21.004446563299688 29.03070257641071 28.801368845185475 21.163482015104123 72-73 21.027259882639395 28.959556597306197 28.640060807849743 21.37312271220467 74-75 20.94549784006411 29.27273873565852 28.42555647295746 21.356206951319905 76-77 21.084952761581643 29.10829944725321 28.53299898932884 21.273748801836305 78-79 20.966317136092595 29.233670131657526 28.33350006517699 21.466512667072887 80-81 20.996304411654084 29.376590886480162 28.298488521352155 21.328616180513595 82-83 21.0641546132728 29.359533094690686 28.41611856465179 21.16019372738473 84-85 21.006829310873673 29.213095594591348 28.203974894129313 21.57610020040566 86-87 21.036097517200485 29.1628744860471 28.2943463816685 21.506681615083913 88-89 21.15024825174613 28.920433905360504 28.263826685288834 21.66549115760453 90-91 20.953365072954437 28.849581241968824 28.66965870507925 21.52739497999749 92-93 21.119937127017614 28.90174216619498 28.50587524006406 21.472445466723347 94-95 20.855138767733557 28.869132234135353 28.44636999758127 21.82935900054982 96-97 20.820233642310235 29.00868732305566 28.457560638484903 21.713518396149205 98-99 20.80086593212014 29.155291706335007 28.142915183525457 21.90092717801939 100-101 20.58204932368448 29.295560528676905 28.128926115175958 21.993464032462658 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 489.0 1 318.0 2 195.5 3 246.0 4 248.5 5 280.0 6 421.5 7 775.0 8 1301.0 9 1583.0 10 1448.5 11 1288.5 12 1352.5 13 1457.5 14 1530.0 15 1634.0 16 1673.5 17 1693.0 18 1767.0 19 1932.5 20 2183.0 21 2520.5 22 2923.5 23 3448.0 24 4089.5 25 4988.5 26 6266.5 27 7715.5 28 8874.0 29 10341.5 30 12526.0 31 14402.5 32 16259.0 33 18455.0 34 21012.0 35 23087.0 36 25439.5 37 27859.0 38 28886.0 39 28901.5 40 28622.0 41 29355.0 42 29993.5 43 30302.0 44 30869.5 45 31074.0 46 30366.0 47 29954.0 48 31277.0 49 30867.0 50 28133.5 51 25734.0 52 23555.0 53 21954.5 54 20507.0 55 18393.0 56 16351.0 57 14485.5 58 12846.0 59 11150.5 60 9143.0 61 7243.5 62 5602.0 63 4283.0 64 3180.0 65 2349.0 66 1681.5 67 1260.5 68 930.0 69 601.0 70 387.0 71 258.0 72 185.5 73 143.5 74 102.0 75 70.0 76 52.0 77 29.0 78 14.0 79 10.0 80 9.0 81 7.0 82 5.5 83 4.5 84 3.0 85 4.0 86 3.0 87 1.5 88 1.5 89 0.5 90 0.5 91 0.5 92 0.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04557990120313968 2 0.0345485953268479 3 0.031033124223414248 4 0.056611207079431465 5 0.11273752159287208 6 0.1352850259114465 7 0.07055186835166834 8 0.10910082734794072 9 0.09006879413279996 10-11 0.08982634784980453 12-13 0.0869776040246083 14-15 0.07934054611025244 16-17 0.06249052944207049 18-19 0.07370367003060885 20-21 0.0667333393944904 22-23 0.0401854714064915 24-25 0.01078885959329636 26-27 0.003879140527926781 28-29 0.007940115768100131 30-31 0.00630360335788102 32-33 0.006970330636118435 34-35 0.008364396763342122 36-37 0.006788495923871868 38-39 0.005212595084401612 40-41 0.009031124041579538 42-43 0.0045458678061641965 44-45 0.0020607934054611026 46-47 0.0022426281177076704 48-49 1.8183471224656787E-4 50-51 0.002666909112949662 52-53 0.007394611631360427 54-55 0.014728611691971998 56-57 0.01036457859805437 58-59 0.005030760372155044 60-61 0.0027881322544473742 62-63 0.00915234718307725 64-65 0.00284874382519623 66-67 0.027335818407734035 68-69 0.05533836409370549 70-71 0.07037003363942176 72-73 0.0038185289571779257 74-75 0.016001454677697972 76-77 0.027335818407734035 78-79 0.0300027275206837 80-81 0.08473497590690063 82-83 0.03163923993090281 84-85 0.011879867866775768 86-87 0.03776100857653726 88-89 0.05570203351819862 90-91 0.048913537594326754 92-93 0.04915598387732218 94-95 0.013516380276994878 96-97 0.019395702639633906 98-99 0.04606479376913052 100-101 0.08685638088311058 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 824925.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.87962594057028 #Duplication Level Percentage of deduplicated Percentage of total 1 85.13841985718143 58.64302512933879 2 9.454572480875525 13.024548318214313 3 2.3979014868407473 4.954996723677841 4 0.9484493343305762 2.613153414890919 5 0.5045902099679609 1.7377992457933482 6 0.30048935338227173 1.241855655605683 7 0.19403755881027349 0.935566412849113 8 0.144663061381628 0.7971470044307434 9 0.1168028886425507 0.7240805350629279 >10 0.7300285930124144 9.343645702682387 >50 0.05046222697527235 2.3404204315064794 >100 0.018347987897356272 1.9917041885869697 >500 7.056918296651832E-4 0.3502191215941953 >1k 5.292688722488874E-4 1.3018381157663481 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4956 0.6007818892626603 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3432 0.41603782162014724 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2325 0.28184380398218023 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 881 0.10679758765948419 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 6.061157074885596E-5 0.0 0.0 0.0 0.0 20-21 1.2122314149771192E-4 0.0 0.0 0.0 0.0 22-23 1.2122314149771192E-4 0.0 0.0 3.6366942449313574E-4 0.0 24-25 1.2122314149771192E-4 0.0 0.0 7.273388489862715E-4 0.0 26-27 1.2122314149771192E-4 0.0 0.0 0.0010303967027305513 0.0 28-29 5.455041367397036E-4 0.0 0.0 0.001636512410219111 0.0 30-31 6.061157074885596E-4 0.0 0.0 0.004364033093917629 0.0 32-33 6.061157074885596E-4 0.0 0.0 0.0075158347728581385 0.0 34-35 6.061157074885596E-4 0.0 0.0 0.010243355456556656 0.0 36-37 6.061157074885596E-4 0.0 0.0 0.013213322423250599 0.0 38-39 7.273388489862715E-4 0.0 0.0 0.016425735672939965 0.0 40-41 7.273388489862715E-4 0.0 0.0 0.02012304148862018 0.0 42-43 8.485619904839834E-4 0.0 0.0 0.02933600024244628 0.0 44-45 9.697851319816953E-4 0.0 0.0 0.04036730611873807 0.0 46-47 0.001212231414977119 0.0 0.0 0.050246992150801584 0.0 48-49 0.001333454556474831 0.0 0.0 0.06443009970603389 0.0 50-51 0.001333454556474831 0.0 0.0 0.08128011637421584 0.0 52-53 0.001333454556474831 0.0 0.0 0.11982907537048823 0.0 54-55 0.001333454556474831 0.0 0.0 0.15092281116465134 0.0 56-57 0.001454677697972543 0.0 0.0 0.2042003818528957 0.0 58-59 0.001454677697972543 0.0 0.0 0.2707518865351396 0.0 60-61 0.001454677697972543 0.0 0.0 0.3390611267691002 0.0 62-63 0.001454677697972543 0.0 0.0 0.4365245325332606 0.0 64-65 0.001454677697972543 0.0 0.0 0.47992241718944145 0.0 66-67 0.001575900839470255 0.0 0.0 0.6049034760735825 0.0 68-69 0.001575900839470255 0.0 0.0 0.7715852956329363 0.0 70-71 0.001575900839470255 0.0 0.0 0.8675334121283753 0.0 72-73 0.001575900839470255 0.0 0.0 1.0507621905021667 0.0 74-75 0.001636512410219111 0.0 0.0 1.2390823408188623 0.0 76-77 0.0016971239809679668 0.0 0.0 1.3433342425068946 0.0 78-79 0.0016971239809679668 0.0 0.0 1.444919235081977 0.0 80-81 0.0019395702639633907 0.0 0.0 1.6575446252689638 0.0 82-83 0.0019395702639633907 0.0 0.0 1.8194987423099072 0.0 84-85 0.0020001818347122466 0.0 0.0 2.1078885959329634 0.0 86-87 0.0020607934054611026 0.0 0.0 2.5410188805042884 0.0 88-89 0.0020607934054611026 0.0 0.0 3.1073127860108496 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGA 70 2.0432216E-4 33.955677 6 GGTATCA 3340 0.0 32.456966 1 GTATCAA 5135 0.0 31.57428 1 GTATAGG 170 3.59978E-9 27.968592 1 TAGGACG 70 0.008345227 27.17279 4 ATCAACG 6370 0.0 25.530478 3 TATCAAC 6350 0.0 25.461119 2 TCAACGC 6385 0.0 25.396025 4 CAACGCA 6455 0.0 25.119097 5 AACGCAG 6645 0.0 24.538023 6 ACGCAGT 140 1.770222E-5 23.764645 7 GAGTACT 3380 0.0 22.922321 12-13 GTACATG 4175 0.0 22.435165 1 TACATGG 4095 0.0 21.598885 2 ACGCAGA 7390 0.0 21.481438 7 CGCAGAG 7400 0.0 21.323952 8 AGTACTT 3555 0.0 20.924852 12-13 GTACTTT 3740 0.0 20.717152 14-15 CTACTCG 115 0.0036987013 20.674948 4 TACCTGG 535 0.0 20.443079 2 >>END_MODULE