FastQCFastQC Report
Thu 26 May 2016
SRR936828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936828_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824925
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69990.8484407673424856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54450.6600600054550414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35920.43543352425978116No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13900.16850016668181955No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9680.11734400096978512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36150.037.9745941
GTATCAA58200.037.0541151
TATCAAC74500.028.6884572
ATCAACG74800.028.5733973
TCAACGC75000.028.49724
CAACGCA75400.028.2830315
AACGCAG77150.027.7097646
ACGCAGA85450.024.7958537
CGCAGAG85250.024.6868448
GAGTACT52000.023.75054612-13
AGTACTT53400.023.08339512-13
GCAGAGT90250.023.0559589
AGAGTAC75150.022.41008610-11
GTACTTT55700.022.21415514-15
TCGCTAA1105.6488716E-821.58878754-55
GTGTAAG3750.021.5338291
GTTCGCG600.00476707319.7897260-61
TAGAACG1456.138057E-419.6580077
TACTTTT62500.019.49326714-15
GACCGCG1101.345179E-619.43226430-31
Produced by FastQC (version 0.11.5)