##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936828_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 824925 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17215989332364 33.0 31.0 34.0 31.0 34.0 2 32.29816771221626 33.0 31.0 34.0 31.0 34.0 3 32.41582204442828 34.0 31.0 34.0 31.0 34.0 4 36.08950510652483 37.0 35.0 37.0 35.0 37.0 5 35.94397551292542 37.0 35.0 37.0 35.0 37.0 6 36.03028032851471 37.0 35.0 37.0 35.0 37.0 7 35.99029972421735 37.0 35.0 37.0 35.0 37.0 8 35.94068188017093 37.0 35.0 37.0 35.0 37.0 9 37.6500360638846 39.0 37.0 39.0 35.0 39.0 10-11 37.5671309512986 39.0 37.0 39.0 35.0 39.0 12-13 37.46771948965058 39.0 37.0 39.0 35.0 39.0 14-15 38.65470073036943 40.0 38.0 41.0 34.5 41.0 16-17 38.65448737764039 40.0 38.0 41.0 34.5 41.0 18-19 38.80357426432706 40.0 38.0 41.0 35.0 41.0 20-21 38.826861835924475 40.0 38.0 41.0 35.0 41.0 22-23 38.74295178349547 40.0 38.0 41.0 34.5 41.0 24-25 38.64987180652787 40.0 38.0 41.0 34.0 41.0 26-27 38.450253053307875 40.0 38.0 41.0 34.0 41.0 28-29 38.29446495135922 40.0 38.0 41.0 34.0 41.0 30-31 38.224617389459645 40.0 37.5 41.0 34.0 41.0 32-33 38.04341909870594 40.0 37.0 41.0 33.5 41.0 34-35 37.90569385095615 40.0 37.0 41.0 33.0 41.0 36-37 37.7349334788011 40.0 37.0 41.0 33.0 41.0 38-39 37.57013849743916 40.0 37.0 41.0 33.0 41.0 40-41 37.2058762917841 40.0 36.0 41.0 32.0 41.0 42-43 37.00312937539776 40.0 36.0 41.0 31.0 41.0 44-45 36.725025911446494 39.0 36.0 41.0 31.0 41.0 46-47 36.261538321665604 39.0 35.5 41.0 30.0 41.0 48-49 35.809152953298785 39.0 35.0 40.5 28.5 41.0 50-51 35.504585871442856 39.0 35.0 40.0 27.5 41.0 52-53 35.25974724974998 39.0 35.0 40.0 27.0 41.0 54-55 34.89370791284056 38.0 34.5 40.0 26.0 41.0 56-57 34.71530320938267 38.0 34.0 40.0 26.0 41.0 58-59 34.413438191350735 38.0 34.0 40.0 25.0 41.0 60-61 34.049189926356945 38.0 34.0 40.0 23.5 41.0 62-63 33.70015152892687 37.0 33.0 40.0 23.0 41.0 64-65 33.4066775767494 37.0 33.0 39.0 22.0 40.0 66-67 33.090001515289266 36.0 32.5 39.0 22.0 40.0 68-69 32.65205079249629 36.0 32.0 39.0 20.5 40.0 70-71 32.21965875685669 35.0 31.5 38.5 19.0 40.0 72-73 31.80025638694427 35.0 31.0 38.0 18.0 40.0 74-75 31.324944085825983 35.0 31.0 37.0 15.0 39.0 76-77 29.88899718156196 33.5 29.5 35.5 13.0 38.0 78-79 30.546251477407036 34.0 30.0 36.0 10.5 38.5 80-81 30.39309028093463 34.0 30.0 36.0 7.5 37.5 82-83 30.024564657393096 34.0 30.0 35.0 6.5 37.0 84-85 29.573441221929265 34.0 29.5 35.0 2.0 36.5 86-87 29.183206958208324 34.0 29.0 35.0 2.0 36.0 88-89 28.797336727581296 34.0 29.0 35.0 2.0 36.0 90-91 28.35503288177713 34.0 29.0 35.0 2.0 35.0 92-93 27.865167742522047 33.0 27.5 35.0 2.0 35.0 94-95 27.326051459223564 33.0 26.5 34.5 2.0 35.0 96-97 26.57278176803952 32.5 25.5 34.0 2.0 35.0 98-99 25.736649998484708 32.0 24.5 34.0 2.0 35.0 100-101 22.621135254720127 28.5 9.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.06235385996511411 1101 2 0.23219529604698153 1101 3 0.14731878723515024 1101 4 0.019159851430309516 1101 5 0.03516117823657794 1101 6 0.0979330804584464 1101 7 0.045891728564811274 1101 8 0.05848854092231903 1101 9 0.21522047167920988 1101 10-11 0.20442368244183484 1101 12-13 0.12431493611385491 1101 14-15 0.3027433228824705 1101 16-17 0.2153558558176698 1101 18-19 0.10238851358634093 1101 20-21 0.11797368939549813 1101 22-23 0.2806336829168359 1101 24-25 0.008116371002138578 1101 26-27 0.025751892832097667 1101 28-29 0.007930919687048288 1101 30-31 0.09942477655721405 1101 32-33 -0.07353803483838561 1101 34-35 -0.175895320686827 1101 36-37 0.02886566937384316 1101 38-39 0.08002622893786082 1101 40-41 0.04772735319896526 1101 42-43 0.02491785916976852 1101 44-45 -0.17274133276924175 1101 46-47 -0.5599187533828669 1101 48-49 -0.32296770535292296 1101 50-51 -0.14988081565503109 1101 52-53 -0.1437161226172421 1101 54-55 -0.4760350292941453 1101 56-57 -0.5911137864438913 1101 58-59 -0.33598712527891195 1101 60-61 -0.1736669951152905 1101 62-63 -0.20329955588540827 1101 64-65 -0.10432805925604782 1101 66-67 0.056783858581155755 1101 68-69 -0.4910928407297632 1101 70-71 -0.6470509068160695 1101 72-73 -0.44690722479973033 1101 74-75 -0.5261385325016512 1101 76-77 -0.44885835753265013 1101 78-79 -0.3350743215146359 1101 80-81 -0.394759037125084 1101 82-83 -0.13463544730542054 1101 84-85 -0.30033321090645515 1101 86-87 -0.5313169641040822 1101 88-89 -0.4305766448308219 1101 90-91 -0.046202979026393365 1101 92-93 -0.08744076701505676 1101 94-95 -0.18200902830857757 1101 96-97 -0.195620874369407 1101 98-99 0.148734819424579 1101 100-101 0.5950368063935727 1102 1 -0.050796663402131514 1102 2 0.14674269395351303 1102 3 0.14236768398683353 1102 4 0.1509599398867465 1102 5 0.001732017098440508 1102 6 0.027149503869914327 1102 7 0.15612134672786482 1102 8 0.15612847408856823 1102 9 0.2316954827362636 1102 10-11 0.30585987095232525 1102 12-13 0.26643187060695794 1102 14-15 0.263195924975939 1102 16-17 0.34745079905813014 1102 18-19 0.38329912344431705 1102 20-21 0.23127778296274926 1102 22-23 0.3026049714322383 1102 24-25 0.20569671893078834 1102 26-27 0.32758029963341073 1102 28-29 0.20977591206996493 1102 30-31 0.17856564343027514 1102 32-33 0.22495482721871696 1102 34-35 0.40343750893624275 1102 36-37 0.4211146742389431 1102 38-39 0.4992022326726868 1102 40-41 0.38811263011674413 1102 42-43 0.6096010745611764 1102 44-45 0.7058829239509876 1102 46-47 0.2849093094212023 1102 48-49 0.3291190065248131 1102 50-51 0.18559942907443627 1102 52-53 0.23875813901723575 1102 54-55 0.2757402422179709 1102 56-57 0.2240675492482822 1102 58-59 0.2156677471963988 1102 60-61 0.39939095613538456 1102 62-63 0.29858167906471067 1102 64-65 0.40505354426255025 1102 66-67 0.2944153146723565 1102 68-69 0.4597677666710993 1102 70-71 0.08031898851048425 1102 72-73 0.0770082624477979 1102 74-75 0.4042634528538933 1102 76-77 0.5677487238964112 1102 78-79 0.3069934705144526 1102 80-81 0.426706391151491 1102 82-83 0.39026442492417246 1102 84-85 0.20994831398420288 1102 86-87 0.17671997874323253 1102 88-89 0.10651823698155027 1102 90-91 -0.06624235442567894 1102 92-93 -0.05727497262712333 1102 94-95 0.09077771796888356 1102 96-97 0.33086844943028737 1102 98-99 0.4101189783513064 1102 100-101 0.4465436371960685 1103 1 0.11185951980176867 1103 2 0.17672531467428598 1103 3 0.08912436269168467 1103 4 0.046288857496129765 1103 5 -0.13373837757371376 1103 6 -0.07534269926166814 1103 7 -0.06120038109751391 1103 8 0.07442723000544049 1103 9 0.009902135718945715 1103 10-11 0.08145756185786723 1103 12-13 0.2230167956607545 1103 14-15 0.2735008335095941 1103 16-17 0.18844178025766212 1103 18-19 0.13062003354303187 1103 20-21 0.10818042070052059 1103 22-23 0.12023433009646567 1103 24-25 0.20725246977465162 1103 26-27 0.17577089826108505 1103 28-29 0.03377681053419934 1103 30-31 0.2738856592051633 1103 32-33 0.3356931308809905 1103 34-35 0.30175837452579657 1103 36-37 0.30912907864928485 1103 38-39 0.11294542302806576 1103 40-41 0.23835479106812585 1103 42-43 0.15231642391160705 1103 44-45 0.0550511717283797 1103 46-47 0.051930186293304814 1103 48-49 0.19348144368147757 1103 50-51 0.1383141263137233 1103 52-53 0.28759815752477635 1103 54-55 0.46537053660991745 1103 56-57 0.04052517806869105 1103 58-59 0.08499606237929669 1103 60-61 0.004758658830674278 1103 62-63 0.1776523275426598 1103 64-65 -0.12096776010462662 1103 66-67 0.19281795292769033 1103 68-69 -0.06952754961833563 1103 70-71 0.31448847322882756 1103 72-73 0.42041050219076226 1103 74-75 0.1712910578567275 1103 76-77 0.2225536960885286 1103 78-79 0.21688413321463074 1103 80-81 0.1573974322831404 1103 82-83 0.462422591891535 1103 84-85 0.31532377990725635 1103 86-87 0.081456375756769 1103 88-89 0.3179611311956698 1103 90-91 0.0716351584017616 1103 92-93 0.20518164386838578 1103 94-95 0.2568880199791437 1103 96-97 0.5971896007066171 1103 98-99 0.3616966214974653 1103 100-101 0.8348385529029407 1104 1 -0.05908233457673617 1104 2 0.06332427912366967 1104 3 -0.09003531484680138 1104 4 0.0018299739083289523 1104 5 -0.0753358487492406 1104 6 0.04353581461507616 1104 7 0.028260102808850718 1104 8 -0.05752465553378272 1104 9 0.03913926011240676 1104 10-11 0.09581761105561526 1104 12-13 0.14496192667814256 1104 14-15 0.02341974713413464 1104 16-17 0.06345261853557105 1104 18-19 -0.04674075747462325 1104 20-21 0.11431247748988937 1104 22-23 -0.01122514973042854 1104 24-25 0.008043316166542525 1104 26-27 0.03505579671310244 1104 28-29 0.1088868792676081 1104 30-31 0.07774654816874005 1104 32-33 0.1871824975937244 1104 34-35 0.2892421706312902 1104 36-37 0.2280512667718213 1104 38-39 0.1878414023213324 1104 40-41 0.4439090204585412 1104 42-43 0.3446677575282493 1104 44-45 0.21488338983413513 1104 46-47 0.10763251205393942 1104 48-49 0.5667078464757793 1104 50-51 0.7457682924407081 1104 52-53 0.5628855993593476 1104 54-55 0.5531466718459299 1104 56-57 0.40411541516203897 1104 58-59 0.2292730769876954 1104 60-61 0.25708072642085966 1104 62-63 0.4197179052790716 1104 64-65 0.29696479958083444 1104 66-67 -0.031802011554880494 1104 68-69 0.044789788736110836 1104 70-71 0.3825394778970477 1104 72-73 0.4388810686393043 1104 74-75 0.2586143939268375 1104 76-77 0.4603048363824094 1104 78-79 0.7496237608488663 1104 80-81 0.5604170567916711 1104 82-83 0.5644474498164307 1104 84-85 0.19874619228847834 1104 86-87 0.37664717761806443 1104 88-89 0.48135151579664637 1104 90-91 0.4823609323083282 1104 92-93 0.5654031836210471 1104 94-95 0.7133732708223874 1104 96-97 0.28106245726990053 1104 98-99 0.09798553091515316 1104 100-101 1.0819239927871642 1105 1 -0.013452853185526692 1105 2 0.139990820613221 1105 3 0.0735885384197772 1105 4 0.15434173349073887 1105 5 0.043633693235769044 1105 6 0.04272660513278481 1105 7 0.16013217502716515 1105 8 0.12856808617212323 1105 9 0.16907431741727663 1105 10-11 0.0914996062657778 1105 12-13 0.1691019296097025 1105 14-15 0.28054182026099284 1105 16-17 0.11130742600307286 1105 18-19 -0.021474160503345274 1105 20-21 0.16528419513392123 1105 22-23 -0.013980459561956593 1105 24-25 -0.007474002491186127 1105 26-27 -0.12575399423707267 1105 28-29 -0.21541230049924565 1105 30-31 -0.017842533317931952 1105 32-33 -0.01477826059194598 1105 34-35 -0.06052711986885839 1105 36-37 -0.2299649343772998 1105 38-39 -0.32094421410125307 1105 40-41 -0.1471900386537257 1105 42-43 0.0773457684719645 1105 44-45 -0.023184101628579867 1105 46-47 -0.19698397958685376 1105 48-49 -0.3677231690215521 1105 50-51 -0.2512165542637561 1105 52-53 -0.10707564822846649 1105 54-55 0.11434071616105257 1105 56-57 0.019400510080615163 1105 58-59 -0.13699112506270694 1105 60-61 0.023173153164982807 1105 62-63 -0.2262335639570381 1105 64-65 -0.2247905793000129 1105 66-67 -0.07514256881916737 1105 68-69 -0.14854041113135708 1105 70-71 -0.21622284346814524 1105 72-73 -0.1916287939041048 1105 74-75 -0.07011363294667206 1105 76-77 0.0032129269024210316 1105 78-79 0.029947614236579767 1105 80-81 -0.19782373633053396 1105 82-83 -0.18428028632313342 1105 84-85 -0.502441092462405 1105 86-87 -0.229310203208275 1105 88-89 -0.16010871967362306 1105 90-91 -0.026953456478064908 1105 92-93 0.07849226813619836 1105 94-95 0.19366998904183674 1105 96-97 0.10134224023701321 1105 98-99 0.21101016940566097 1105 100-101 0.5902341380693379 1106 1 -0.10737091067709059 1106 2 -0.03140234484423843 1106 3 -0.09226909684814188 1106 4 -0.05904023594408869 1106 5 -0.13095939843445592 1106 6 0.13162683318889634 1106 7 -0.019502548412035026 1106 8 0.2020920067734906 1106 9 0.2756180251960316 1106 10-11 0.15940766380196436 1106 12-13 0.24380852865791525 1106 14-15 0.1990954417116555 1106 16-17 0.06092160505314581 1106 18-19 0.07075169983944107 1106 20-21 0.0720839267626161 1106 22-23 0.17489700751272608 1106 24-25 0.016990333431145643 1106 26-27 0.13046920024435593 1106 28-29 0.1649363441810081 1106 30-31 0.16438218153755457 1106 32-33 0.20620111617369474 1106 34-35 0.19626956208585256 1106 36-37 0.1506983473991781 1106 38-39 0.20048458047418904 1106 40-41 0.21949085982485883 1106 42-43 0.3694323851636412 1106 44-45 0.03490028656963062 1106 46-47 0.03751735408764034 1106 48-49 0.11232973166470117 1106 50-51 -0.14291816821074121 1106 52-53 0.026304446772591916 1106 54-55 0.1313080608471111 1106 56-57 -0.029897097933627492 1106 58-59 -0.09150263423479288 1106 60-61 -0.16102046096646205 1106 62-63 -0.09157409352920354 1106 64-65 -0.3750294208404412 1106 66-67 -0.42786191104168125 1106 68-69 -0.6416771570250859 1106 70-71 -0.4523152143739537 1106 72-73 -0.5664872815930906 1106 74-75 -0.3587801518405165 1106 76-77 -0.2660911374947901 1106 78-79 -0.28096590444750547 1106 80-81 -0.3062924578328179 1106 82-83 -0.22305651909805135 1106 84-85 -0.3506781577536948 1106 86-87 -0.47763176678356345 1106 88-89 -0.24517454056403665 1106 90-91 -0.3693016756916059 1106 92-93 -0.33694473526902513 1106 94-95 -0.22452213446847225 1106 96-97 -0.37656324367186755 1106 98-99 -0.28309949687074365 1106 100-101 0.014752475535846798 1107 1 0.3080390335978862 1107 2 0.2936685753150172 1107 3 0.31109157437038704 1107 4 0.177196865362518 1107 5 0.1523892921895822 1107 6 0.2672250330473389 1107 7 0.23903219435810996 1107 8 0.22270320786623898 1107 9 0.1560085773144806 1107 10-11 0.3400981123302387 1107 12-13 0.36948458330315503 1107 14-15 0.3215102182869245 1107 16-17 0.4897372240855091 1107 18-19 0.37460380436058216 1107 20-21 0.2747543783814308 1107 22-23 0.35616263904076817 1107 24-25 0.35440717990211823 1107 26-27 0.44849007471328406 1107 28-29 0.3250728237558107 1107 30-31 0.3764444272122347 1107 32-33 0.28673913643768856 1107 34-35 0.4475472695952831 1107 36-37 0.420709087963985 1107 38-39 0.377744916121614 1107 40-41 0.6505900412144925 1107 42-43 0.4200818745575745 1107 44-45 0.20962333070895767 1107 46-47 0.13550259201117854 1107 48-49 0.2476074315431731 1107 50-51 0.030289178106663428 1107 52-53 0.21985781860407627 1107 54-55 0.32323890153000434 1107 56-57 0.1545973801187941 1107 58-59 0.06432998846614879 1107 60-61 0.0541761982027964 1107 62-63 0.2740324431441934 1107 64-65 0.23630712130816534 1107 66-67 0.42279463698276487 1107 68-69 0.10067519836360361 1107 70-71 0.21409486153493873 1107 72-73 0.4190797267494446 1107 74-75 0.39350865679619673 1107 76-77 0.23794849766940018 1107 78-79 0.38864249417816055 1107 80-81 0.4283850676784091 1107 82-83 0.7256482222031408 1107 84-85 0.4325656072816635 1107 86-87 0.521632800226687 1107 88-89 0.5849433004107389 1107 90-91 0.7108519353702221 1107 92-93 0.8100712763758047 1107 94-95 1.0742162784079952 1107 96-97 0.8337143131262614 1107 98-99 0.7898829271392813 1107 100-101 1.3907800309031764 1108 1 0.004395689103716904 1108 2 0.07289894723449919 1108 3 0.0676878447009841 1108 4 -0.045060874080739666 1108 5 -0.14004265019305961 1108 6 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-1.0034178935292886 2316 72-73 -1.0647455770449064 2316 74-75 -0.8624864457334702 2316 76-77 -1.039596808378029 2316 78-79 -1.1951125492972672 2316 80-81 -1.0373252285711132 2316 82-83 -0.9196809488733244 2316 84-85 -0.5690278493185055 2316 86-87 -0.434685736935684 2316 88-89 -1.0512861217648393 2316 90-91 -1.06702317913631 2316 92-93 -1.019734959301612 2316 94-95 -1.1493679168741835 2316 96-97 -0.8518203619716438 2316 98-99 -1.3391937277580404 2316 100-101 -2.0462665751083833 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 5.0 5 13.0 6 37.0 7 97.0 8 236.0 9 446.0 10 796.0 11 1305.0 12 2079.0 13 3016.0 14 4011.0 15 5547.0 16 7634.0 17 11152.0 18 10251.0 19 5649.0 20 4451.0 21 4382.0 22 4625.0 23 4882.0 24 5801.0 25 6822.0 26 8191.0 27 10119.0 28 12641.0 29 15237.0 30 19464.0 31 24578.0 32 30920.0 33 40504.0 34 55874.0 35 80102.0 36 121267.0 37 185588.0 38 129697.0 39 7504.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42415057004531 20.16419341892601 16.022498693557754 21.38915731747092 2 15.183413000811019 21.377594749341426 41.00888970246834 22.430102547379217 3 18.644747879804687 26.18556376102526 31.459989041414726 23.709699317755334 4 12.331060399430251 18.416219656332395 38.79310240324878 30.459617540988575 5 12.715934741338971 37.969480010958875 34.79576967930736 14.51881556839479 6 29.392490226384215 38.053883686395736 18.193775191684093 14.359850895535958 7 26.342426696277478 34.41129122197923 21.781036798541443 17.465245283201845 8 25.634694063096646 34.770070006364215 20.517986483619723 19.077249446919417 9 25.956299057490074 16.99172652059278 21.50619753310907 35.54577688880807 10-11 23.574143103918537 27.707306724853776 28.145528381368003 20.573021789859684 12-13 25.432971349423077 25.286096605150615 28.836185863533174 20.444746181893127 14-15 22.3733044167145 26.199341617564787 28.12799846769398 23.29935549802673 16-17 20.489862714792253 29.54129163257266 28.5482316574234 21.420613995211685 18-19 20.135964782018124 27.25617469340948 32.43280101876607 20.175059505806324 20-21 21.20233139454537 26.275405678652564 32.56321265004594 19.95905027675612 22-23 20.818430165284997 26.41947561456816 32.58991754843113 20.17217667171571 24-25 20.704492556854 26.422865247539157 32.726567424719974 20.146074770886873 26-27 20.62655430890948 26.63716497691607 32.59377070265218 20.142510011522266 28-29 20.324829726841667 26.70044349513198 32.74651195351817 20.228214824508182 30-31 20.613522813299884 26.49884928863187 32.60480201521472 20.282825882853523 32-33 20.529903681977785 26.527611348203806 32.53192568765467 20.41055928216374 34-35 20.729399642391733 26.55308058308331 32.513561838955056 20.2039579355699 36-37 20.72068904904201 26.36921827361939 32.48416484322437 20.425927834114233 38-39 20.55001536677491 26.715820140234705 32.55537646173794 20.17878803125244 40-41 20.67998026477687 26.636853710840192 32.3027295947127 20.380436429670233 42-43 20.651089492984212 26.676970633693976 32.03169985150165 20.640240021820166 44-45 20.770455604792982 26.858419872604976 31.65788696889193 20.713237553710112 46-47 20.889467530507485 27.25389901208662 31.17391312253726 20.68272033486863 48-49 21.00648059700045 27.56763047262536 30.62039654562117 20.80549238475302 50-51 20.969266064777354 27.626416455278047 30.475237982670677 20.92907949727392 52-53 21.197410905158854 27.878349636904844 29.968669746151605 20.955569711784698 54-55 21.072703579113252 27.64124011273752 30.08800800072734 21.19804830742189 56-57 21.18099435887085 27.966714489282946 29.870466776616254 20.981824375229944 58-59 21.2516933408896 27.629504589825103 30.181077616382147 20.93772445290315 60-61 21.19768797899397 27.513740959576 30.48044262012884 20.80812844130119 62-63 21.00281843803982 27.663242112919356 30.50113646695154 20.83280298208928 64-65 20.921308556116347 27.722294585746937 30.299560747680545 21.05683611045617 66-67 20.854926205412614 27.97145195017729 29.99630269418432 21.177319150225777 68-69 21.013463672692072 27.68971910728692 30.205891819059588 21.09092540096142 70-71 21.062913259019194 27.82240491974085 30.14741402666799 20.967267794571967 72-73 21.05125026896466 27.689085944437203 29.92481111862023 21.334852667977902 74-75 21.014256888263358 27.958314921999587 29.739683440225146 21.287744749511912 76-77 20.969191726030687 27.92607081011656 29.787513887634564 21.317223576218186 78-79 20.92532440965595 27.90217008527478 29.728101401163627 21.444404103905647 80-81 20.953832203504895 28.027806872072386 29.57041061747791 21.447950306944808 82-83 20.97847801736887 28.040825579083652 29.686068049905202 21.294628353642274 84-85 20.85100708566441 28.050563398209487 29.522308629478545 21.576120886647555 86-87 20.791468289445373 27.877467277229073 29.514530152832542 21.816534280493016 88-89 20.851335428122546 27.719104283649976 29.50864585454792 21.920914433679556 90-91 20.676071704574728 27.610261694422135 29.856196869364933 21.857469731638204 92-93 20.788556535442616 27.44146437554929 29.86344213110283 21.906536957905264 94-95 20.48586235112283 27.518198624117346 29.867563717913747 22.128375306846078 96-97 20.59993514542275 27.360933905912372 29.916022414226788 22.12310853443808 98-99 20.472992180439643 27.535871725509853 29.623471894496245 22.36766419955426 100-101 20.034682802675945 27.64713975981462 29.699134869551568 22.619042567957866 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 613.0 1 421.5 2 293.0 3 346.5 4 277.5 5 263.5 6 490.0 7 1121.5 8 1961.5 9 2341.5 10 1983.5 11 1554.0 12 1497.0 13 1581.5 14 1701.0 15 1809.0 16 1942.5 17 2052.5 18 2148.5 19 2281.5 20 2532.5 21 2854.0 22 3202.5 23 3712.5 24 4316.5 25 5200.5 26 6444.0 27 7850.0 28 9040.5 29 10297.5 30 12229.0 31 14014.5 32 15804.5 33 17791.5 34 20405.5 35 22909.0 36 25031.5 37 27096.5 38 28213.5 39 28419.0 40 28772.5 41 29440.5 42 29367.0 43 29790.5 44 30629.5 45 30917.5 46 30310.5 47 29859.5 48 31262.5 49 30898.0 50 27913.0 51 25633.0 52 23538.0 53 21850.0 54 20255.5 55 18192.5 56 16226.5 57 14252.5 58 12820.0 59 11366.5 60 9388.0 61 7449.5 62 5728.0 63 4356.0 64 3117.5 65 2175.5 66 1648.5 67 1295.5 68 947.5 69 574.5 70 378.0 71 259.0 72 175.0 73 127.5 74 98.0 75 68.0 76 44.5 77 23.0 78 12.5 79 10.5 80 4.5 81 2.5 82 2.5 83 0.5 84 0.0 85 0.0 86 0.5 87 1.5 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020123041488620177 2 0.004606479376913053 3 1.212231414977119E-4 4 0.0 5 0.0027881322544473742 6 0.0 7 1.212231414977119E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.008788677758584114 14-15 0.002969966966693942 16-17 0.0 18-19 6.667272782374155E-4 20-21 0.001333454556474831 22-23 0.0020001818347122466 24-25 6.061157074885596E-4 26-27 6.061157074885595E-5 28-29 6.061157074885595E-5 30-31 6.061157074885595E-5 32-33 1.8183471224656787E-4 34-35 0.0 36-37 0.002424462829954238 38-39 0.011334363730036065 40-41 4.848925659908476E-4 42-43 0.0 44-45 0.0012728429857259752 46-47 5.455041367397036E-4 48-49 1.212231414977119E-4 50-51 0.002727520683698518 52-53 4.2428099524199176E-4 54-55 0.0 56-57 1.8183471224656787E-4 58-59 3.030578537442798E-4 60-61 0.0023032396884565265 62-63 0.0 64-65 6.061157074885595E-5 66-67 0.0 68-69 1.8183471224656787E-4 70-71 4.2428099524199176E-4 72-73 3.030578537442798E-4 74-75 0.0030911901081916537 76-77 6.061157074885595E-5 78-79 4.2428099524199176E-4 80-81 0.0025456859714519504 82-83 1.212231414977119E-4 84-85 0.002424462829954238 86-87 3.030578537442798E-4 88-89 0.0025456859714519504 90-91 0.001515289268721399 92-93 0.0 94-95 0.0 96-97 3.030578537442798E-4 98-99 7.879504197351274E-4 100-101 0.0024850744007030943 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 824925.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.28514083690031 #Duplication Level Percentage of deduplicated Percentage of total 1 83.50042728156947 54.51337155018615 2 10.220798751821066 13.345325719565066 3 2.7164296310603695 5.32027473111916 4 1.1438838756274212 2.987144796855822 5 0.6071044172717812 1.9817448692146264 6 0.3738197742561639 1.4642925965959164 7 0.2635693329187912 1.2045012713923802 8 0.1784324486936149 0.931919003426851 9 0.13456821632536134 0.7906774459474514 >10 0.7772274085313409 9.575160568902898 >50 0.05601441925772823 2.489114388303636 >100 0.026225824154224402 2.9024723599938844 >500 7.493092563231371E-4 0.36285490474368814 >1k 3.7465462816156853E-4 0.6092831599032877 >5k 3.7465462816156853E-4 1.5218626338491592 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6999 0.8484407673424856 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5445 0.6600600054550414 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3592 0.43543352425978116 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1390 0.16850016668181955 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 968 0.11734400096978512 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.2122314149771192E-4 0.0 0.0 0.0 0.0 7 3.6366942449313574E-4 0.0 0.0 0.0 0.0 8 3.6366942449313574E-4 0.0 0.0 0.0 0.0 9 3.6366942449313574E-4 0.0 0.0 0.0 0.0 10-11 3.6366942449313574E-4 0.0 0.0 0.0 0.0 12-13 3.6366942449313574E-4 0.0 0.0 0.0 0.0 14-15 3.6366942449313574E-4 0.0 0.0 0.0 0.0 16-17 4.242809952419917E-4 0.0 0.0 0.0 0.0 18-19 7.273388489862715E-4 0.0 0.0 0.0 0.0 20-21 7.273388489862715E-4 0.0 0.0 1.2122314149771192E-4 0.0 22-23 7.273388489862715E-4 0.0 0.0 5.455041367397036E-4 0.0 24-25 7.273388489862715E-4 0.0 0.0 9.697851319816953E-4 0.0 26-27 7.879504197351274E-4 0.0 0.0 0.001333454556474831 0.0 28-29 0.0011516198442282632 0.0 0.0 0.0020001818347122466 0.0 30-31 0.001212231414977119 0.0 0.0 0.004848925659908476 0.0 32-33 0.001212231414977119 0.0 0.0 0.0081219504803467 0.0 34-35 0.001212231414977119 0.0 0.0 0.01109191744704064 0.0 36-37 0.001333454556474831 0.0 0.0 0.014183107555232295 0.0 38-39 0.001454677697972543 0.0 0.0 0.01721368609267509 0.0 40-41 0.001454677697972543 0.0 0.0 0.02072915719610874 0.0 42-43 0.001454677697972543 0.0 0.0 0.02951783495469285 0.0 44-45 0.0018183471224656788 0.0 0.0 0.04048852926023578 0.0 46-47 0.0018183471224656788 0.0 0.0 0.05048943843379701 0.0 48-49 0.0018183471224656788 0.0 0.0 0.0646725459890293 0.0 50-51 0.0018183471224656788 0.0 0.0 0.0820074552232021 0.0 52-53 0.0018183471224656788 0.0 0.0 0.12031396793647908 0.0 54-55 0.0018183471224656788 0.0 0.0 0.15128648058914446 0.0 56-57 0.0019395702639633907 0.0 0.0 0.20420038185289574 0.0 58-59 0.0020607934054611026 0.0 0.0 0.27063066339364183 0.0 60-61 0.0020607934054611026 0.0 0.0 0.3386974573446071 0.0 62-63 0.0020607934054611026 0.0 0.0 0.4361002515380186 0.0 64-65 0.0020607934054611026 0.0 0.0 0.47974058247719487 0.0 66-67 0.0021820165469588143 0.0 0.0 0.6052065339273267 0.0 68-69 0.0023032396884565265 0.0 0.0 0.7720095766281783 0.0 70-71 0.0023032396884565265 0.0 0.0 0.8668666848501378 0.0 72-73 0.0023032396884565265 0.0 0.0 1.0503379095069247 0.0 74-75 0.002363851259205382 0.0 0.0 1.2385974482528714 0.0 76-77 0.002424462829954238 0.0 0.0 1.3426675152286571 0.0 78-79 0.002424462829954238 0.0 0.0 1.4443737309452374 0.0 80-81 0.002424462829954238 0.0 0.0 1.6585750219716946 0.0 82-83 0.002424462829954238 0.0 0.0 1.8197411885929022 0.0 84-85 0.0024850744007030943 0.0 0.0 2.105888414098251 0.0 86-87 0.0025456859714519504 0.0 0.0 2.5366548474103707 0.0 88-89 0.0025456859714519504 0.0 0.0 3.1014334636482106 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3615 0.0 37.974594 1 GTATCAA 5820 0.0 37.054115 1 TATCAAC 7450 0.0 28.688457 2 ATCAACG 7480 0.0 28.573397 3 TCAACGC 7500 0.0 28.4972 4 CAACGCA 7540 0.0 28.283031 5 AACGCAG 7715 0.0 27.709764 6 ACGCAGA 8545 0.0 24.795853 7 CGCAGAG 8525 0.0 24.686844 8 GAGTACT 5200 0.0 23.750546 12-13 AGTACTT 5340 0.0 23.083395 12-13 GCAGAGT 9025 0.0 23.055958 9 AGAGTAC 7515 0.0 22.410086 10-11 GTACTTT 5570 0.0 22.214155 14-15 TCGCTAA 110 5.6488716E-8 21.588787 54-55 GTGTAAG 375 0.0 21.533829 1 GTTCGCG 60 0.004767073 19.78972 60-61 TAGAACG 145 6.138057E-4 19.658007 7 TACTTTT 6250 0.0 19.493267 14-15 GACCGCG 110 1.345179E-6 19.432264 30-31 >>END_MODULE