FastQCFastQC Report
Thu 26 May 2016
SRR936827_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936827_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647113
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106190.6447037938502095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73820.44817811528413654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48600.29506172314832074No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17210.10448584887618519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58200.043.8520661
GTATCAA101850.039.617351
ATCAACG125950.031.7725943
TCAACGC126550.031.6219524
TATCAAC127950.031.2759552
CAACGCA129350.030.9723015
AACGCAG132450.030.2832766
ACGCAGA149650.026.7065897
CGCAGAG149900.026.5661338
CGCGGAA851.1229713E-725.14978682-83
GCAGAGT155450.025.0368189
GAGTACT82000.024.1038412-13
GTACATG94000.023.5106091
TACATGG94850.023.099492
GTACTTT86450.022.69994414-15
AGTACTT85250.022.0981312-13
ACATGGG95750.021.7903653
CAGAGTA152400.020.54598410-11
TCGCGGA1058.608731E-720.3593582-83
ATTCGCG1058.61628E-720.35749480-81
Produced by FastQC (version 0.11.5)