##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936827_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1647113 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.758418517733755 33.0 31.0 34.0 30.0 34.0 2 31.845074381660517 33.0 31.0 34.0 30.0 34.0 3 31.853776274001845 33.0 31.0 34.0 30.0 34.0 4 35.43856128875189 37.0 35.0 37.0 33.0 37.0 5 35.477176732865324 37.0 35.0 37.0 33.0 37.0 6 35.53804990914406 37.0 35.0 37.0 33.0 37.0 7 35.478253768867106 37.0 35.0 37.0 33.0 37.0 8 35.448802237612114 37.0 35.0 37.0 33.0 37.0 9 37.06235941310645 39.0 37.0 39.0 33.0 39.0 10-11 36.93742293333851 39.0 37.0 39.0 33.0 39.0 12-13 36.832448350538186 39.0 37.0 39.0 33.0 39.0 14-15 37.95139950932328 40.0 38.0 41.0 33.0 41.0 16-17 37.88583236244265 40.0 38.0 41.0 33.0 41.0 18-19 37.886201493158026 40.0 38.0 41.0 33.0 41.0 20-21 37.84631412659605 40.0 38.0 41.0 32.5 41.0 22-23 37.7124814751629 40.0 37.0 41.0 32.0 41.0 24-25 37.548325160447405 40.0 37.0 41.0 32.0 41.0 26-27 37.50139547195609 40.0 37.0 41.0 32.0 41.0 28-29 37.55980858629614 40.0 37.0 41.0 32.0 41.0 30-31 37.402932889243175 40.0 37.0 41.0 32.0 41.0 32-33 37.19809266273777 40.0 36.0 41.0 31.0 41.0 34-35 36.999516426620396 39.0 36.0 41.0 31.0 41.0 36-37 36.833179326494296 39.0 36.0 41.0 30.5 41.0 38-39 36.664361825812804 39.0 35.0 41.0 30.0 41.0 40-41 36.448746382306496 39.0 35.0 41.0 30.0 41.0 42-43 36.219524404215136 39.0 35.0 41.0 30.0 41.0 44-45 35.925572501704494 39.0 35.0 40.5 29.0 41.0 46-47 35.52194081401822 39.0 35.0 40.0 27.0 41.0 48-49 35.13615611072221 38.5 35.0 40.0 25.5 41.0 50-51 34.46790475213298 37.5 33.5 39.5 24.0 40.5 52-53 34.760644533799436 38.5 34.5 40.0 25.5 40.5 54-55 35.0721902504564 39.0 35.0 40.5 26.0 41.0 56-57 34.96961592799037 39.0 35.0 41.0 25.5 41.0 58-59 34.74417966466174 38.5 34.5 40.0 25.0 41.0 60-61 34.475846526619605 38.0 34.0 40.0 23.5 41.0 62-63 34.16923246917486 38.0 34.0 40.0 23.0 41.0 64-65 33.87364558472916 37.0 34.0 40.0 22.5 41.0 66-67 33.518674796446874 37.0 33.5 40.0 22.0 41.0 68-69 33.09230210677713 36.0 33.0 39.0 20.0 41.0 70-71 32.74954602386114 36.0 32.5 39.0 20.0 40.5 72-73 32.375432347386 35.0 32.0 38.5 19.0 40.0 74-75 31.942450821528336 35.0 32.0 37.5 17.5 39.5 76-77 31.507419648803697 35.0 31.5 37.0 14.5 39.0 78-79 31.083733781470976 35.0 31.0 36.5 10.0 39.0 80-81 30.671601159119014 35.0 31.0 36.0 7.5 38.5 82-83 30.261678464076233 34.5 30.5 35.5 6.5 37.0 84-85 29.908616166589663 34.0 30.0 35.0 2.0 37.0 86-87 29.576442235596467 34.0 30.0 35.0 2.0 36.0 88-89 29.20421549705454 34.0 29.5 35.0 2.0 36.0 90-91 28.867133888203178 34.0 29.0 35.0 2.0 36.0 92-93 28.53053312067842 34.0 29.0 35.0 2.0 35.0 94-95 28.15242761122036 34.0 29.0 35.0 2.0 35.0 96-97 27.73753986520658 34.0 28.5 35.0 2.0 35.0 98-99 27.161998903536066 34.0 27.0 35.0 2.0 35.0 100-101 25.059483775551527 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4813264715719363 1101 2 0.37661752952718786 1101 3 0.5349523339953706 1101 4 0.3978745551473537 1101 5 0.32810898983399284 1101 6 0.28034660400981437 1101 7 0.4021718081777479 1101 8 0.37178239185426065 1101 9 0.36801883014993564 1101 10-11 0.4037697563506697 1101 12-13 0.5443284014844636 1101 14-15 0.7831703209604726 1101 16-17 0.7467538149009485 1101 18-19 0.7133245326305016 1101 20-21 0.7401666568978555 1101 22-23 0.668674011122171 1101 24-25 0.7524461206440662 1101 26-27 0.8334708061395304 1101 28-29 0.7338290233982789 1101 30-31 0.6113843430341319 1101 32-33 0.7683309847255941 1101 34-35 0.7261596881291723 1101 36-37 0.8241934245271594 1101 38-39 0.8340648393941237 1101 40-41 0.8226557211065426 1101 42-43 0.8820632805868982 1101 44-45 0.852274287556618 1101 46-47 0.916870526993506 1101 48-49 1.0929958925041419 1101 50-51 1.0271418053493804 1101 52-53 1.0438554993252112 1101 54-55 1.1386472296718892 1101 56-57 1.1134284503265306 1101 58-59 1.2719323555900885 1101 60-61 1.3472872384503845 1101 62-63 1.3423697945020123 1101 64-65 1.290211684894686 1101 66-67 1.1877384327057001 1101 68-69 1.1625573126139273 1101 70-71 1.117959903646664 1101 72-73 1.067091372387054 1101 74-75 1.0845458962606322 1101 76-77 1.1894783592286764 1101 78-79 1.157373861209166 1101 80-81 1.0222073832145533 1101 82-83 0.8944578224419999 1101 84-85 0.9261619294277352 1101 86-87 1.0376713517328398 1101 88-89 0.9951702611311148 1101 90-91 0.8930583854869205 1101 92-93 0.7289077677761249 1101 94-95 0.91883732273131 1101 96-97 0.9295231106359374 1101 98-99 0.7967247753354414 1101 100-101 0.850158901227843 1102 1 0.357281151750211 1102 2 0.4366597894169928 1102 3 0.31282659495057175 1102 4 0.21244051247555262 1102 5 0.27585283203070077 1102 6 0.1793293624703125 1102 7 0.1363727416428091 1102 8 0.16523737399949567 1102 9 0.2725322297405057 1102 10-11 0.3837781881974962 1102 12-13 0.36243395365041664 1102 14-15 0.5487771739072045 1102 16-17 0.4726632387479768 1102 18-19 0.4160607017691689 1102 20-21 0.31218829476668475 1102 22-23 0.41522384496290954 1102 24-25 0.33665115776278043 1102 26-27 0.4826796526876578 1102 28-29 0.37294559193859556 1102 30-31 0.33414305129687705 1102 32-33 0.5234982976531839 1102 34-35 0.37885605957021795 1102 36-37 0.40521038732792647 1102 38-39 0.31299141651339113 1102 40-41 0.3499111433492672 1102 42-43 0.20420183387090418 1102 44-45 0.42172572197713265 1102 46-47 0.38214064805902836 1102 48-49 0.16982887070367525 1102 50-51 0.2227610389417336 1102 52-53 0.30033656212504667 1102 54-55 0.4559438587914144 1102 56-57 0.5032538669635187 1102 58-59 0.39144488025072377 1102 60-61 0.4183017390589612 1102 62-63 0.5032486964572129 1102 64-65 0.604870942272747 1102 66-67 0.4297688276165488 1102 68-69 0.3717888686264814 1102 70-71 0.4255589410688856 1102 72-73 0.47221490010451816 1102 74-75 0.46231353964848054 1102 76-77 0.4987326014774105 1102 78-79 0.5796616945729554 1102 80-81 0.6441400956831593 1102 82-83 0.6271154337129516 1102 84-85 0.6931376992886449 1102 86-87 0.8195898407830562 1102 88-89 0.7841929102021439 1102 90-91 0.676982051334921 1102 92-93 0.6441922903431241 1102 94-95 0.8210971970511132 1102 96-97 0.5668185860867929 1102 98-99 0.6529532698675133 1102 100-101 1.039491414862809 1103 1 0.4037240344385715 1103 2 0.4839234438910651 1103 3 0.3716918269662983 1103 4 0.42578455968223494 1103 5 0.3138003399122553 1103 6 0.3131155151992715 1103 7 0.31777033946112 1103 8 0.31873048939811355 1103 9 0.33435245118008794 1103 10-11 0.36692036563400876 1103 12-13 0.3488673063835961 1103 14-15 0.48444575652857225 1103 16-17 0.512434449491856 1103 18-19 0.65816007001402 1103 20-21 0.4950535408487582 1103 22-23 0.3327482369007768 1103 24-25 0.7366409596557588 1103 26-27 0.6883033812210826 1103 28-29 0.7526059949748074 1103 30-31 0.6949271098973142 1103 32-33 0.7072803183905094 1103 34-35 0.5156828306561252 1103 36-37 0.6849783979761526 1103 38-39 0.7328411526813241 1103 40-41 0.7735160686833069 1103 42-43 0.798646502986287 1103 44-45 0.8731206647710295 1103 46-47 0.6932564088817657 1103 48-49 0.6937026392383032 1103 50-51 0.5469127938073726 1103 52-53 0.6568029532083841 1103 54-55 0.7686350513193716 1103 56-57 0.7849932771964419 1103 58-59 0.743352541666539 1103 60-61 0.6534850324497157 1103 62-63 0.8308031024790168 1103 64-65 0.7732501289912932 1103 66-67 0.6778977387224643 1103 68-69 0.8952269318301305 1103 70-71 0.896392882479006 1103 72-73 0.7746353680569698 1103 74-75 0.7579517389441115 1103 76-77 0.7150578366932372 1103 78-79 0.8059089846018423 1103 80-81 0.8241266623146011 1103 82-83 0.8592106348002844 1103 84-85 0.8357786221821684 1103 86-87 0.9852194068536022 1103 88-89 0.9177729908375198 1103 90-91 1.0822335489621047 1103 92-93 0.9931460173116697 1103 94-95 1.1178934089818426 1103 96-97 0.9687421600661459 1103 98-99 0.994968239985301 1103 100-101 1.3904703452220488 1104 1 0.4242835882749496 1104 2 0.4854820028661351 1104 3 0.47281973599385196 1104 4 0.3593178926731042 1104 5 0.43207753464309206 1104 6 0.41666122830592656 1104 7 0.3798856440581986 1104 8 0.3488979034950077 1104 9 0.3194682300914238 1104 10-11 0.4578589085474647 1104 12-13 0.5142481875807192 1104 14-15 0.628229414433271 1104 16-17 0.5763232099563069 1104 18-19 0.5447239628840705 1104 20-21 0.36270530856833716 1104 22-23 0.5468081443324451 1104 24-25 0.5276990229125005 1104 26-27 0.6370500443261093 1104 28-29 0.48531484307610384 1104 30-31 0.5254820233306745 1104 32-33 0.4501912038170772 1104 34-35 0.5591848109609501 1104 36-37 0.6404765906162382 1104 38-39 0.579599463026689 1104 40-41 0.6734405551139773 1104 42-43 0.7188947559858505 1104 44-45 0.7410679799297313 1104 46-47 0.8066961118959739 1104 48-49 0.7630807738647789 1104 50-51 0.6713507656601649 1104 52-53 0.8514397774603921 1104 54-55 0.7830064308204285 1104 56-57 0.6693881669312205 1104 58-59 0.7682189374432582 1104 60-61 0.8258075113495735 1104 62-63 0.6332837489162628 1104 64-65 0.5992142968608718 1104 66-67 0.6604590699530632 1104 68-69 0.7744754262676423 1104 70-71 0.7191781174357956 1104 72-73 0.572313796689798 1104 74-75 0.6070514836363223 1104 76-77 0.6053395314163659 1104 78-79 0.6335443824864342 1104 80-81 0.8117857587887514 1104 82-83 0.8076595244425988 1104 84-85 0.8548356100982843 1104 86-87 0.7026865002318665 1104 88-89 0.7630098996784298 1104 90-91 0.7233458073315226 1104 92-93 0.6687090728543801 1104 94-95 0.779488851455401 1104 96-97 0.6389791973314978 1104 98-99 0.751867800088796 1104 100-101 0.9596224528383033 1105 1 0.3515026479418246 1105 2 0.3866607734946186 1105 3 0.3423882867768242 1105 4 0.22815426397627192 1105 5 0.30540665123596256 1105 6 0.29407674974422093 1105 7 0.2866650126971493 1105 8 0.28937286180677546 1105 9 0.39012163319264914 1105 10-11 0.4400930143109818 1105 12-13 0.4074515686398854 1105 14-15 0.563485198076144 1105 16-17 0.6784929145928089 1105 18-19 0.494688503930206 1105 20-21 0.6544347471668459 1105 22-23 0.5338071668153361 1105 24-25 0.6419592724668632 1105 26-27 0.5775230859376776 1105 28-29 0.5737820579190256 1105 30-31 0.4061578518243394 1105 32-33 0.37448729566534666 1105 34-35 0.44923138677602736 1105 36-37 0.38346817630232266 1105 38-39 0.43985476749451635 1105 40-41 0.760175072968039 1105 42-43 0.5395932015419831 1105 44-45 0.4208046253210327 1105 46-47 0.505331421347222 1105 48-49 0.6297493572316526 1105 50-51 0.44488113577497046 1105 52-53 0.43729261321835367 1105 54-55 0.614547649458423 1105 56-57 0.6222603421942878 1105 58-59 0.6594308669853604 1105 60-61 0.5295910775619816 1105 62-63 0.4836143797023098 1105 64-65 0.49752185057639764 1105 66-67 0.6744183975746267 1105 68-69 0.7168517511866526 1105 70-71 0.7292197098303319 1105 72-73 0.7425666094473513 1105 74-75 0.8219269939577565 1105 76-77 0.6235884341054891 1105 78-79 0.6963929823369313 1105 80-81 0.6923638355717578 1105 82-83 0.7027949393123549 1105 84-85 0.7887132862909496 1105 86-87 0.8674652171927448 1105 88-89 0.8710102063504621 1105 90-91 0.7073605275891275 1105 92-93 0.6321058489646312 1105 94-95 0.7559709398919487 1105 96-97 0.716038212530929 1105 98-99 0.7192053118285813 1105 100-101 0.7861898344341469 1106 1 0.429809875701995 1106 2 0.5017279176484024 1106 3 0.4236909486158922 1106 4 0.29816413142811626 1106 5 0.43179466949964507 1106 6 0.4739562442301093 1106 7 0.5445468128842208 1106 8 0.38059162064348584 1106 9 0.5437430275683965 1106 10-11 0.5586119869095256 1106 12-13 0.6305556435613369 1106 14-15 0.8545758184600558 1106 16-17 0.7042006916872694 1106 18-19 0.6760024372072522 1106 20-21 0.7786392703751304 1106 22-23 0.8067644164262759 1106 24-25 0.7197622516858928 1106 26-27 0.7841932065420636 1106 28-29 0.7190143385264136 1106 30-31 0.733798779404367 1106 32-33 0.701709400339432 1106 34-35 0.8315172343980564 1106 36-37 0.7745067898758933 1106 38-39 0.8698263120572349 1106 40-41 0.7818979023758175 1106 42-43 0.9416260152686178 1106 44-45 1.0096207881676023 1106 46-47 0.7511379520579311 1106 48-49 0.9368996015450151 1106 50-51 0.834844414616505 1106 52-53 0.8361508363255936 1106 54-55 0.8526271150736164 1106 56-57 0.888188762630783 1106 58-59 1.0301184049275918 1106 60-61 1.1016244647477436 1106 62-63 1.084588073748023 1106 64-65 1.1251176924045012 1106 66-67 1.1713359559043184 1106 68-69 1.197257048367021 1106 70-71 1.2160461672569198 1106 72-73 1.1278459137030126 1106 74-75 1.0668213291703204 1106 76-77 1.1985205582969662 1106 78-79 1.3075352931399138 1106 80-81 1.41731128496399 1106 82-83 1.389429386804263 1106 84-85 1.3842818314646976 1106 86-87 1.4427544679980606 1106 88-89 1.5075662481378345 1106 90-91 1.6191644915758943 1106 92-93 1.6000123078940796 1106 94-95 1.7163063541568384 1106 96-97 1.5016309418601992 1106 98-99 1.6871619978676193 1106 100-101 1.6473108877005913 1107 1 0.1952758639731087 1107 2 0.3474427871798582 1107 3 0.3268066640984273 1107 4 0.24928818381987128 1107 5 0.1993264948332225 1107 6 0.17151565492505938 1107 7 0.31831701660721734 1107 8 0.3496465665966113 1107 9 0.4581372734663489 1107 10-11 0.39039604461400756 1107 12-13 0.4059950681511282 1107 14-15 0.4341890103928634 1107 16-17 0.5817480465576139 1107 18-19 0.5278849848686136 1107 20-21 0.5599186181345885 1107 22-23 0.6095354170597176 1107 24-25 0.48438351489110687 1107 26-27 0.5097713752827389 1107 28-29 0.4057664176453173 1107 30-31 0.5451314588624641 1107 32-33 0.4149565038764891 1107 34-35 0.5765823154172764 1107 36-37 0.49656690553008076 1107 38-39 0.5787794986773136 1107 40-41 0.4932444766826052 1107 42-43 0.5475697411314329 1107 44-45 0.6482337553308071 1107 46-47 0.887599261034417 1107 48-49 0.8059565908582371 1107 50-51 0.6623786919822052 1107 52-53 0.7292769729446533 1107 54-55 0.8135114813254845 1107 56-57 0.8030423558795263 1107 58-59 0.7916889315014899 1107 60-61 0.7511551049324581 1107 62-63 0.7375439984706347 1107 64-65 0.8280497098139321 1107 66-67 0.7495796878972101 1107 68-69 0.9825800014310317 1107 70-71 0.8280075886182061 1107 72-73 0.7447855732792235 1107 74-75 0.8920247456684187 1107 76-77 0.8565991867936624 1107 78-79 0.911280567869678 1107 80-81 0.9150715579569031 1107 82-83 0.9439875101823461 1107 84-85 1.0097494544238899 1107 86-87 0.9984916101546233 1107 88-89 0.9660639697913247 1107 90-91 0.9175169303261761 1107 92-93 1.0292319486713772 1107 94-95 0.9101840386211748 1107 96-97 0.9610528752875283 1107 98-99 1.1341613235587893 1107 100-101 0.9740490719708035 1108 1 0.05212349721418619 1108 2 0.289046647800113 1108 3 0.10725020962394183 1108 4 0.1046114397988589 1108 5 0.07511174783159191 1108 6 0.1029193878041994 1108 7 0.13367717557393632 1108 8 -0.006415458809676977 1108 9 0.0017282180409026182 1108 10-11 0.08198677628671192 1108 12-13 -0.1093601231057022 1108 14-15 0.126387222017307 1108 16-17 0.13533650727722346 1108 18-19 0.19481281114070725 1108 20-21 0.3315409302487069 1108 22-23 0.20931435396980191 1108 24-25 0.262818331440684 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14 8594.0 15 10866.0 16 14224.0 17 18987.0 18 16614.0 19 9959.0 20 9146.0 21 9280.0 22 9892.0 23 11074.0 24 12752.0 25 14645.0 26 17090.0 27 20310.0 28 24240.0 29 29396.0 30 36076.0 31 44984.0 32 57564.0 33 75700.0 34 102278.0 35 146521.0 36 219121.0 37 323914.0 38 309614.0 39 58949.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.705159872330555 19.64910341006703 14.805063524906355 21.840673192696062 2 14.950426155786149 21.01779067470868 41.06428039683384 22.967502772671335 3 18.53707761819157 26.632434038297852 30.462158709058905 24.368329634451666 4 12.128111118473726 17.501901277062423 38.84313597945709 31.526851625006763 5 12.900700633536083 38.42222530157387 34.19569360775219 14.481380457137858 6 29.48914611748375 37.62302464686441 18.49341376757777 14.394415468074062 7 26.7422410722643 34.34873145132763 21.548369686117624 17.360657790290446 8 25.894880519526296 34.503333031499864 20.579897040350676 19.02188940862316 9 26.946539829194904 16.61544540011549 20.9836792997599 35.454335470929706 10-11 23.898716266789684 27.56305238165133 27.938572379755044 20.59965897180394 12-13 25.540906798252276 25.290811086735904 28.302655007079615 20.865627107932205 14-15 22.742244634781034 25.98920939820335 27.43842927342714 23.830116693588476 16-17 20.950684322041624 29.413175159456333 27.804632408626382 21.831508109875656 18-19 20.93141751358065 27.44441875353018 31.109850534582012 20.514313198307153 20-21 22.221607898688465 26.52483286433782 30.973389225973612 20.280170011000102 22-23 21.631367588279595 26.741903722937092 30.724734630341683 20.901994058441627 24-25 21.209936956188272 26.94353698413349 31.003005630050833 20.8435204296274 26-27 21.110725710335476 27.04280297461903 30.87959256320944 20.966878751836056 28-29 20.916134974319593 27.062543058878425 31.10263535524258 20.918686611559405 30-31 21.151027439489223 27.01790160465098 30.9483155021907 20.8827554536691 32-33 20.99158799680613 27.066076608544666 30.760651979601267 21.18168341504794 34-35 21.331662160229136 27.07637538467706 30.702825070147664 20.889137384946142 36-37 21.400165101201935 26.8420051960106 30.632347322390164 21.1254823803973 38-39 21.171363115762592 27.196651321797905 30.607865153865287 21.024120408574216 40-41 21.421454746930802 26.9470459869553 30.639137027712703 20.992362238401196 42-43 21.17237633705068 27.10217749338271 30.333911074093585 21.391535095473028 44-45 21.342191581121302 27.416145295511146 30.018253052299094 21.223410071068457 46-47 21.567077518466466 27.445645242204968 29.495723651046042 21.491553588282525 48-49 21.55178356833932 27.616374504859607 29.25482111776984 21.57702080903123 50-51 21.71002649626787 27.844371015767194 28.918007320330346 21.527595167634594 52-53 21.734285943353594 27.99370028619602 28.755259884452606 21.516753885997776 54-55 21.642936302643157 27.91378408117312 28.799310700272763 21.64396891591096 56-57 21.633403431531555 28.252459207586643 28.493702703457895 21.62043465742391 58-59 21.698944758204384 27.898786450673473 28.740562143603032 21.66170664751911 60-61 21.72115500557797 27.886261065958536 28.879361271673666 21.51322265678983 62-63 21.727047842838033 27.824133192555973 28.98311643656696 21.465702528039035 64-65 21.644179991794317 27.748213623563135 28.916476025703048 21.691130358939507 66-67 21.618878757843444 28.094023202952783 28.556800394643993 21.73029764455978 68-69 21.710400428620634 28.03392153253744 28.670474198022006 21.58520384081992 70-71 21.653764941436673 27.89639024970866 28.619755277136587 21.830089531718084 72-73 21.6319120892734 28.052787243325767 28.46705488317525 21.848245784225583 74-75 21.584837343419583 28.298801528970642 28.302445405618126 21.813915721991645 76-77 21.58550466539276 28.195712041827576 28.405019989334164 21.8137633034455 78-79 21.597420507150407 28.20574584158536 28.247329987971277 21.949503663292955 80-81 21.50974841879162 28.528895412028266 28.087520608777755 21.87383556040236 82-83 21.707893304432773 28.512475517950264 28.072952863245575 21.706678314371388 84-85 21.699811093624845 28.306643739100824 27.907993269963583 22.085551897310747 86-87 21.619980467889725 28.26533293438821 28.084714073295537 22.02997252442653 88-89 21.91858486667801 27.971609829610365 28.170742082107875 21.939063221603753 90-91 21.59005856479794 28.216629000510316 28.260431095234622 21.932881339457122 92-93 21.657492702194553 28.1124619996154 28.210525548318934 22.019519749871115 94-95 21.389357607737313 28.132377005102267 28.273255965860926 22.20500942129949 96-97 21.51318014305416 28.022171845475253 28.170901746809147 22.29374626466144 98-99 21.336684669460798 28.04740094157551 28.18465578394216 22.431258605021533 100-101 21.480320282815583 28.22858535678054 28.079391139279746 22.211703221124125 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 600.0 1 461.0 2 354.0 3 410.0 4 410.0 5 450.5 6 714.5 7 1422.0 8 2409.0 9 2866.5 10 2538.5 11 2270.5 12 2433.5 13 2640.5 14 2803.5 15 2896.0 16 3016.0 17 3236.0 18 3377.0 19 3586.5 20 4093.5 21 4699.0 22 5456.5 23 6462.0 24 7675.0 25 9357.5 26 11936.5 27 14847.0 28 17062.5 29 19615.5 30 23071.0 31 26530.5 32 30574.0 33 34792.0 34 39072.5 35 43012.5 36 47821.5 37 52488.0 38 53750.5 39 53755.5 40 53724.0 41 54188.0 42 54744.5 43 56130.5 44 58376.5 45 59543.5 46 59240.0 47 58663.5 48 61322.0 49 60728.0 50 56072.0 51 53097.0 52 50053.5 53 47775.5 54 45504.5 55 42062.5 56 38363.0 57 34370.0 58 31350.5 59 28381.0 60 24089.5 61 19769.5 62 15754.5 63 11674.0 64 8154.0 65 5809.0 66 3950.5 67 2721.0 68 1918.0 69 1306.0 70 930.0 71 695.5 72 532.0 73 436.5 74 319.5 75 241.0 76 182.0 77 90.0 78 48.0 79 29.5 80 20.0 81 18.0 82 13.0 83 9.0 84 9.0 85 7.0 86 5.5 87 2.5 88 3.0 89 2.5 90 2.5 91 2.0 92 2.0 93 2.0 94 1.5 95 2.0 96 1.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05992302896036884 2 0.09641111447727023 3 0.06799776335928379 4 0.13150281735375774 5 0.10594294380531269 6 0.10387872598904872 7 0.1121355972541046 8 0.09798963398382503 9 0.11917822274488757 10-11 0.10894820209663818 12-13 0.09367906148515615 14-15 0.09756464796282951 16-17 0.11140704978954084 18-19 0.08994525572926691 20-21 0.07403863608629159 22-23 0.07579929245898732 24-25 0.06286757496298068 26-27 0.0557945933278409 28-29 0.06745135276086098 30-31 0.050755473364608256 32-33 0.05066440493153779 34-35 0.04641454472158255 36-37 0.05160544540659931 38-39 0.05169651383966977 40-41 0.06562998409945159 42-43 0.06350505399447397 44-45 0.04935909072419439 46-47 0.03733805755889244 48-49 0.04471460063760046 50-51 0.028291926540559145 52-53 0.04183076692370226 54-55 0.048843036270128405 56-57 0.05166615769531296 58-59 0.05767667427796393 60-61 0.05491426514149302 62-63 0.06162297304435093 64-65 0.04210397222291367 66-67 0.0651139296453856 68-69 0.054762484419708914 70-71 0.026349133301722467 72-73 0.021340369482846653 74-75 0.031266828687527816 76-77 0.04462353220452999 78-79 0.0639300400154695 80-81 0.040464740427645224 82-83 0.06125869931206905 84-85 0.0643246698921082 86-87 0.06711743517293592 88-89 0.08942920127520092 90-91 0.0651442857897424 92-93 0.0753743064379918 94-95 0.08341868469254994 96-97 0.09182733667938993 98-99 0.12130315284986518 100-101 0.117903264681901 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1647113.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.62515938683792 #Duplication Level Percentage of deduplicated Percentage of total 1 76.79174204775902 45.01928117142816 2 13.6255428638447 15.975992442501752 3 4.39675223489186 7.732809016649133 4 1.8564320306412092 4.3533449474868835 5 0.9573746596024348 2.8063121006056226 6 0.5663251516106957 1.9920541366771314 7 0.3634698097789413 1.4915932871415867 8 0.23602042911318716 1.1069388220248384 9 0.17451786789451496 0.9208024039050358 >10 0.9183773419573028 9.972003475887718 >50 0.07627861074242633 3.0383531590630777 >100 0.03560982365475812 3.600338747703264 >500 9.342771053459267E-4 0.3524891769756626 >1k 4.152342690426341E-4 0.5421833697645335 >5k 1.0380856726065852E-4 0.44925329841753137 >10k+ 1.0380856726065852E-4 0.6462504437680527 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10619 0.6447037938502095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7382 0.44817811528413654 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4860 0.29506172314832074 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1721 0.10448584887618519 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.071228871364624E-5 0.0 0.0 0.0 0.0 6 6.071228871364624E-5 0.0 0.0 0.0 0.0 7 6.071228871364624E-5 0.0 0.0 0.0 0.0 8 6.071228871364624E-5 0.0 0.0 0.0 0.0 9 6.071228871364624E-5 0.0 0.0 6.071228871364624E-5 0.0 10-11 6.071228871364624E-5 0.0 0.0 6.071228871364624E-5 0.0 12-13 6.071228871364624E-5 0.0 0.0 6.071228871364624E-5 0.0 14-15 6.071228871364624E-5 0.0 0.0 6.071228871364624E-5 0.0 16-17 6.071228871364624E-5 0.0 0.0 6.071228871364624E-5 0.0 18-19 9.106843307046937E-5 0.0 0.0 6.071228871364624E-5 0.0 20-21 1.2142457742729249E-4 0.0 0.0 1.2142457742729249E-4 0.0 22-23 1.2142457742729249E-4 0.0 0.0 3.035614435682312E-4 0.0 24-25 1.821368661409387E-4 0.0 0.0 6.678351758501086E-4 0.0 26-27 1.821368661409387E-4 0.0 0.0 9.410404750615168E-4 0.0 28-29 2.4284915485458497E-4 0.0 0.0 0.001760656372695741 0.0 30-31 2.4284915485458497E-4 0.0 0.0 0.0036427373228187744 0.0 32-33 2.4284915485458497E-4 0.0 0.0 0.006192653448791916 0.0 34-35 2.732052992114081E-4 0.0 0.0 0.00913719945140376 0.0 36-37 3.035614435682312E-4 0.0 0.0 0.012324594608870188 0.0 38-39 3.035614435682312E-4 3.035614435682312E-5 0.0 0.01447988085820463 0.0 40-41 3.035614435682312E-4 6.071228871364624E-5 0.0 0.01760656372695741 0.0 42-43 3.339175879250543E-4 6.071228871364624E-5 0.0 0.023192094288612866 0.0 44-45 3.642737322818774E-4 6.071228871364624E-5 0.0 0.030295432068109474 0.0 46-47 3.642737322818774E-4 6.071228871364624E-5 0.0 0.04771985892892595 0.0 48-49 3.642737322818774E-4 6.071228871364624E-5 0.0 0.08338832854819311 0.0 50-51 3.642737322818774E-4 6.071228871364624E-5 0.0 0.1105570777475498 0.0 52-53 3.9462987663870056E-4 6.071228871364624E-5 0.0 0.13505448624350608 0.0 54-55 4.249860209955237E-4 6.071228871364624E-5 0.0 0.17327287198874636 0.0 56-57 4.553421653523468E-4 6.071228871364624E-5 0.0 0.1992880877025438 0.0 58-59 4.8569830970916994E-4 1.2142457742729249E-4 0.0 0.22821749327459623 0.0 60-61 4.8569830970916994E-4 1.2142457742729249E-4 0.0 0.2785783367625658 0.0 62-63 4.8569830970916994E-4 1.2142457742729249E-4 0.0 0.3113022603792211 0.0 64-65 4.8569830970916994E-4 1.821368661409387E-4 0.0 0.3500367005785274 0.0 66-67 4.8569830970916994E-4 1.821368661409387E-4 0.0 0.42953944264904714 0.0 68-69 4.8569830970916994E-4 1.821368661409387E-4 0.0 0.521397135472794 0.0 70-71 5.464105984228162E-4 1.821368661409387E-4 0.0 0.5983499614173404 0.0 72-73 5.464105984228162E-4 1.821368661409387E-4 0.0 0.6917558176032852 0.0 74-75 5.767667427796393E-4 1.821368661409387E-4 0.0 0.7715317649730165 0.0 76-77 6.071228871364624E-4 1.821368661409387E-4 0.0 0.83045304116961 0.0 78-79 6.071228871364624E-4 1.821368661409387E-4 0.0 0.9067683880826635 0.0 80-81 7.58903608920578E-4 1.821368661409387E-4 0.0 1.0386051230243463 0.0 82-83 7.892597532774011E-4 1.821368661409387E-4 0.0 1.1651902449922986 0.0 84-85 8.196158976342242E-4 1.821368661409387E-4 0.0 1.3132978733092386 0.0 86-87 8.499720419910474E-4 1.821368661409387E-4 0.0 1.5964903440140414 0.0 88-89 8.499720419910474E-4 1.821368661409387E-4 0.0 1.933777463962703 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5820 0.0 43.852066 1 GTATCAA 10185 0.0 39.61735 1 ATCAACG 12595 0.0 31.772594 3 TCAACGC 12655 0.0 31.621952 4 TATCAAC 12795 0.0 31.275955 2 CAACGCA 12935 0.0 30.972301 5 AACGCAG 13245 0.0 30.283276 6 ACGCAGA 14965 0.0 26.706589 7 CGCAGAG 14990 0.0 26.566133 8 CGCGGAA 85 1.1229713E-7 25.149786 82-83 GCAGAGT 15545 0.0 25.036818 9 GAGTACT 8200 0.0 24.10384 12-13 GTACATG 9400 0.0 23.510609 1 TACATGG 9485 0.0 23.09949 2 GTACTTT 8645 0.0 22.699944 14-15 AGTACTT 8525 0.0 22.09813 12-13 ACATGGG 9575 0.0 21.790365 3 CAGAGTA 15240 0.0 20.545984 10-11 TCGCGGA 105 8.608731E-7 20.35935 82-83 ATTCGCG 105 8.61628E-7 20.357494 80-81 >>END_MODULE