FastQCFastQC Report
Thu 26 May 2016
SRR936827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936827_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647113
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115500.7012269346426141No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90050.5467141598663844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58940.3578382296782309No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22760.13818116911225883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59300.048.3095781
GTATCAA104750.039.7301981
ATCAACG131700.031.4140473
TCAACGC133600.030.9663524
TATCAAC135000.030.8572582
CAACGCA136600.030.286275
AACGCAG140750.029.3932846
ACGCAGA158650.025.8374147
CGCAGAG159350.025.6046838
GCAGAGT166700.024.0483429
GTACATG94650.023.1393381
GAGTACT98100.023.11972812-13
TACATGG96300.022.1961562
GTACTTT102950.021.8690714-15
ACATGGG93950.021.6896233
AGTACTT102300.021.03298412-13
CAGAGTA163700.020.54295510-11
AGAGTAC159500.019.83315810-11
CATGGGA63500.019.4481354
ATATCCG1250.00603345418.9993329
Produced by FastQC (version 0.11.5)