##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936827_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1647113 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14682477765642 33.0 31.0 34.0 31.0 34.0 2 32.134546324386974 33.0 31.0 34.0 30.0 34.0 3 32.30610771695688 33.0 31.0 34.0 30.0 34.0 4 36.0465220054726 37.0 35.0 37.0 35.0 37.0 5 35.98464768355298 37.0 35.0 37.0 35.0 37.0 6 36.03197473397393 37.0 35.0 37.0 35.0 37.0 7 35.97161700502637 37.0 35.0 37.0 35.0 37.0 8 35.951266852972445 37.0 35.0 37.0 35.0 37.0 9 37.63489693785429 39.0 37.0 39.0 35.0 39.0 10-11 37.5659299028057 39.0 37.0 39.0 35.0 39.0 12-13 37.4733640011341 39.0 37.0 39.0 35.0 39.0 14-15 38.65983117126755 40.0 38.0 41.0 34.5 41.0 16-17 38.58464689429323 40.0 38.0 41.0 34.0 41.0 18-19 38.676040441669755 40.0 38.0 41.0 34.5 41.0 20-21 38.77261214015067 40.0 38.0 41.0 35.0 41.0 22-23 38.703821170739346 40.0 38.0 41.0 34.0 41.0 24-25 38.608033267905725 40.0 38.0 41.0 34.0 41.0 26-27 38.448063065497024 40.0 38.0 41.0 34.0 41.0 28-29 38.2705861710763 40.0 38.0 41.0 34.0 41.0 30-31 38.09622199569793 40.0 37.0 41.0 34.0 41.0 32-33 38.02487989591485 40.0 37.0 41.0 33.5 41.0 34-35 37.921156289823465 40.0 37.0 41.0 33.0 41.0 36-37 37.743877013902505 40.0 37.0 41.0 33.0 41.0 38-39 37.565330065393205 40.0 37.0 41.0 33.0 41.0 40-41 37.36042578742321 40.0 36.5 41.0 33.0 41.0 42-43 37.10592351587293 40.0 36.0 41.0 32.0 41.0 44-45 36.71335907129626 40.0 36.0 41.0 31.0 41.0 46-47 36.3438479934285 39.0 36.0 41.0 30.0 41.0 48-49 35.94292862724051 39.0 35.5 41.0 29.0 41.0 50-51 35.66122057199476 39.0 35.0 41.0 28.0 41.0 52-53 35.40268457598234 39.0 35.0 40.0 27.0 41.0 54-55 35.17648060576293 39.0 35.0 40.0 26.5 41.0 56-57 34.914210196871736 38.0 34.5 40.0 26.0 41.0 58-59 34.61465455011283 38.0 34.0 40.0 26.0 41.0 60-61 34.31189693724717 38.0 34.0 40.0 25.0 41.0 62-63 33.95255243568596 37.0 34.0 40.0 24.0 41.0 64-65 33.56539472398069 37.0 33.0 39.5 23.0 41.0 66-67 33.20471849836653 36.0 33.0 39.0 22.0 40.5 68-69 32.78451933777464 36.0 32.0 39.0 21.5 40.0 70-71 32.32520264244165 35.0 32.0 38.0 20.0 40.0 72-73 31.993206294892943 35.0 31.5 38.0 19.5 40.0 74-75 31.55557845758002 35.0 31.0 37.0 18.0 39.0 76-77 30.143326232019298 33.5 29.5 35.5 15.5 38.5 78-79 30.85052847011711 34.0 31.0 36.0 16.0 39.0 80-81 30.685802977694912 34.0 31.0 36.0 12.0 37.5 82-83 30.349378275807425 34.0 31.0 35.5 7.5 37.0 84-85 29.968161565114233 34.0 30.0 35.0 7.0 37.0 86-87 29.61330582661906 34.0 30.0 35.0 2.0 36.0 88-89 29.260223494077213 34.0 29.5 35.0 2.0 36.0 90-91 28.846829877488673 34.0 29.0 35.0 2.0 35.5 92-93 28.414421172075016 34.0 29.0 35.0 2.0 35.0 94-95 27.98177113531373 34.0 29.0 35.0 2.0 35.0 96-97 27.39939154144251 33.0 27.0 35.0 2.0 35.0 98-99 26.705808891071833 33.0 25.5 35.0 2.0 35.0 100-101 23.751809377984387 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35081076254032695 1101 2 0.2656811813285884 1101 3 0.27324364540803003 1101 4 0.2448094925056239 1101 5 0.2502406026409574 1101 6 0.2624915878696612 1101 7 0.29284328856633124 1101 8 0.31045472061086343 1101 9 0.36301470843684314 1101 10-11 0.35105762134138985 1101 12-13 0.37797439118482146 1101 14-15 0.48042562597004945 1101 16-17 0.5418915136343259 1101 18-19 0.519329689022868 1101 20-21 0.3639063135649181 1101 22-23 0.45051283069093984 1101 24-25 0.4279515290690057 1101 26-27 0.3168161963475953 1101 28-29 0.36569532091744605 1101 30-31 0.5447645583550695 1101 32-33 0.547223141831104 1101 34-35 0.46479497161436 1101 36-37 0.4830948516867508 1101 38-39 0.5532554681356103 1101 40-41 0.4296765686355002 1101 42-43 0.5100532137121974 1101 44-45 0.6503961216301306 1101 46-47 0.6640356611003924 1101 48-49 0.41372666894925203 1101 50-51 0.26988827727729614 1101 52-53 0.22692783858081356 1101 54-55 0.2673822180010106 1101 56-57 0.3385169862933921 1101 58-59 0.3495190719064567 1101 60-61 0.3133679887235914 1101 62-63 0.4639694537238128 1101 64-65 0.3811235304907399 1101 66-67 0.40630916947154816 1101 68-69 0.28659054145096974 1101 70-71 0.2697416725511843 1101 72-73 0.3775191114309777 1101 74-75 0.23628369257136583 1101 76-77 0.11422814528366487 1101 78-79 0.26977154423153493 1101 80-81 0.7152641704654812 1101 82-83 0.649043852999938 1101 84-85 0.4902703648352613 1101 86-87 0.5295831824668831 1101 88-89 0.5860117184667111 1101 90-91 0.6686673741008136 1101 92-93 0.7837773917825395 1101 94-95 0.8892723630854604 1101 96-97 0.8473417497943281 1101 98-99 0.8717902047122585 1101 100-101 0.8449329032572344 1102 1 0.18503902788722115 1102 2 0.0191015379283499 1102 3 0.1680228688419021 1102 4 0.14038699271468147 1102 5 0.194773529428673 1102 6 0.22977153137860995 1102 7 0.17455818219653452 1102 8 0.24251718596209315 1102 9 0.23500974998439972 1102 10-11 0.2562661256602823 1102 12-13 0.29928876821922756 1102 14-15 0.3853712641733651 1102 16-17 0.35718451450460975 1102 18-19 0.3222265587868307 1102 20-21 0.306289080697276 1102 22-23 0.29560493835842294 1102 24-25 0.3101244059315178 1102 26-27 0.25613961225985804 1102 28-29 0.264588457130877 1102 30-31 0.22105848680593 1102 32-33 0.4794400748997347 1102 34-35 0.43866669914415013 1102 36-37 0.3797083308727096 1102 38-39 0.4059372057929025 1102 40-41 0.38749971974596775 1102 42-43 0.3995623393087726 1102 44-45 0.5207169886314844 1102 46-47 0.47444550770116933 1102 48-49 0.44172868515946107 1102 50-51 0.546689974796216 1102 52-53 0.6955950880179174 1102 54-55 0.6485286007464666 1102 56-57 0.5632704186512498 1102 58-59 0.44637013715301066 1102 60-61 0.4968980909587657 1102 62-63 0.6363776867370063 1102 64-65 0.4521779062243638 1102 66-67 0.5543657023332997 1102 68-69 0.4155492122388438 1102 70-71 0.5294394578170554 1102 72-73 0.5771014678882693 1102 74-75 0.4028806100306319 1102 76-77 0.5495489503430839 1102 78-79 0.7041618651837069 1102 80-81 0.5589229028855804 1102 82-83 0.4031018244632847 1102 84-85 0.5303309285688727 1102 86-87 0.4436569336399039 1102 88-89 0.5879485655599304 1102 90-91 0.8383809390763659 1102 92-93 0.6402435885163378 1102 94-95 0.43122176890738473 1102 96-97 0.7106910512440798 1102 98-99 0.8580642575425408 1102 100-101 1.0803837089670445 1103 1 0.04763817370784551 1103 2 -0.10691752549860212 1103 3 0.009260848702403734 1103 4 0.09045600576649804 1103 5 0.14209573510339624 1103 6 0.15124434902767092 1103 7 0.23833327525426284 1103 8 0.29416461981945474 1103 9 0.26878095721168904 1103 10-11 0.25305424943717725 1103 12-13 0.3643148876812603 1103 14-15 0.37324606658503257 1103 16-17 0.3584431015744727 1103 18-19 0.3483331852706115 1103 20-21 0.3196484250645 1103 22-23 0.3771936627686472 1103 24-25 0.3617572459460803 1103 26-27 0.2773131682167005 1103 28-29 0.40791159821817047 1103 30-31 0.3970117021108308 1103 32-33 0.35291515019869024 1103 34-35 0.24021188818716155 1103 36-37 0.28987742603687394 1103 38-39 0.34470337550698815 1103 40-41 0.3312471372511965 1103 42-43 0.39466935789219093 1103 44-45 0.46987362276384914 1103 46-47 0.4456432402048236 1103 48-49 0.39768306093860417 1103 50-51 0.22696068178877482 1103 52-53 0.3154887815436993 1103 54-55 0.43988236428753424 1103 56-57 0.5435713683059973 1103 58-59 0.3436911999364156 1103 60-61 0.26771708686721496 1103 62-63 0.33287534029324917 1103 64-65 0.29407208152278486 1103 66-67 0.15183481349794192 1103 68-69 0.17335472027161103 1103 70-71 0.2702814697980713 1103 72-73 0.32735088193668815 1103 74-75 0.35468013634614337 1103 76-77 0.44251172901075364 1103 78-79 0.4560535261212628 1103 80-81 0.49236319912300885 1103 82-83 0.42423951121611836 1103 84-85 0.3789883829455327 1103 86-87 0.24955970348093715 1103 88-89 0.3055464542001225 1103 90-91 0.5513079188718066 1103 92-93 0.7387149274398759 1103 94-95 0.5469509423508114 1103 96-97 0.6538070652612014 1103 98-99 0.670285282170969 1103 100-101 0.8222323444427424 1104 1 0.24276592177676548 1104 2 0.1422676642527847 1104 3 0.17347015656317666 1104 4 0.11538370280810994 1104 5 0.09615608773977868 1104 6 0.04583185829562808 1104 7 0.18312789033197419 1104 8 0.2018579285637898 1104 9 0.17248557415584287 1104 10-11 0.1257007003850319 1104 12-13 0.229040290548717 1104 14-15 0.2945758245820258 1104 16-17 0.3514660587269347 1104 18-19 0.30612794932067544 1104 20-21 0.27904267267708605 1104 22-23 0.1908842215176918 1104 24-25 0.2537992116276371 1104 26-27 0.12184562620905837 1104 28-29 0.2874722496554156 1104 30-31 0.22784366986431337 1104 32-33 0.16367883039787046 1104 34-35 0.16523359191712217 1104 36-37 0.1735926936477199 1104 38-39 0.30140790169787124 1104 40-41 0.29480610375981087 1104 42-43 0.25539065802695404 1104 44-45 0.41626943372992997 1104 46-47 0.4681647769830093 1104 48-49 0.47169474927475363 1104 50-51 0.4664999976461104 1104 52-53 0.38436195128142003 1104 54-55 0.32524643044495605 1104 56-57 0.5508804311329456 1104 58-59 0.41039451037595853 1104 60-61 0.42708427973160923 1104 62-63 0.7062592899782629 1104 64-65 0.5492899664236717 1104 66-67 0.6977777142806474 1104 68-69 0.5969147826687689 1104 70-71 0.6167456025649685 1104 72-73 0.49967301837138933 1104 74-75 0.4299675074294136 1104 76-77 0.26670072868712325 1104 78-79 0.24787973874760993 1104 80-81 0.428530945411115 1104 82-83 0.502748109660299 1104 84-85 0.6417670425711179 1104 86-87 0.4176407512873652 1104 88-89 0.6036783067140696 1104 90-91 0.6107792211469736 1104 92-93 0.7821980721605186 1104 94-95 0.715577365935701 1104 96-97 0.9228461593723445 1104 98-99 0.8426834458727406 1104 100-101 0.8476936022145587 1105 1 0.15614257253481867 1105 2 -0.05366509790388818 1105 3 0.05233470335941348 1105 4 0.13093197490287167 1105 5 0.11151268981365092 1105 6 0.13689428522143743 1105 7 0.11012077937419917 1105 8 0.06853226203129026 1105 9 0.1450729277678633 1105 10-11 0.19945532442428515 1105 12-13 0.21844285509359906 1105 14-15 0.3519618288370978 1105 16-17 0.34519844904377095 1105 18-19 0.36466589040131225 1105 20-21 0.2775303962122493 1105 22-23 0.3521342933939451 1105 24-25 0.2858176886176551 1105 26-27 0.3395559361394831 1105 28-29 0.34759146840840316 1105 30-31 0.3743600672345906 1105 32-33 0.4038451999760042 1105 34-35 0.35081808964583416 1105 36-37 0.304142978277703 1105 38-39 0.24222211594662468 1105 40-41 -0.02584634099130767 1105 42-43 0.04841195256626918 1105 44-45 0.2722255796291435 1105 46-47 0.09535125274633316 1105 48-49 0.2126373824374994 1105 50-51 0.13792774763172844 1105 52-53 0.43916807705528527 1105 54-55 0.38907063070178793 1105 56-57 0.4469780295075836 1105 58-59 0.32286839533561107 1105 60-61 0.3512998701711112 1105 62-63 0.4738651397473035 1105 64-65 0.4518315109006963 1105 66-67 0.40072559920196227 1105 68-69 0.353735360362208 1105 70-71 0.2743980281490863 1105 72-73 0.4010204586182553 1105 74-75 0.47656440620847107 1105 76-77 0.5597833576331332 1105 78-79 0.524610998211319 1105 80-81 0.7597003242085769 1105 82-83 0.7931646086157009 1105 84-85 0.7667358961927206 1105 86-87 0.8831420642270054 1105 88-89 0.734259258547393 1105 90-91 0.8676592165468904 1105 92-93 0.8869380717771769 1105 94-95 0.8683805399912501 1105 96-97 1.0331752567982129 1105 98-99 1.0959945454836522 1105 100-101 1.0666440171801561 1106 1 0.3412322397901093 1106 2 0.2556457030998658 1106 3 0.2805378186266765 1106 4 0.22376472456028296 1106 5 0.2542937292775207 1106 6 0.28508007806822633 1106 7 0.2597362651435162 1106 8 0.2849221193190701 1106 9 0.3978798124412748 1106 10-11 0.37123105049274585 1106 12-13 0.37471323513880606 1106 14-15 0.5823945136343056 1106 16-17 0.531544980505906 1106 18-19 0.43708369294549243 1106 20-21 0.4383844943686839 1106 22-23 0.4194052014170353 1106 24-25 0.4880510152935926 1106 26-27 0.3416023942384143 1106 28-29 0.41667430952265505 1106 30-31 0.49410090325889655 1106 32-33 0.37949652100831344 1106 34-35 0.3988106860907763 1106 36-37 0.3803098192891241 1106 38-39 0.4618735714452029 1106 40-41 0.40016635816215285 1106 42-43 0.5331059330251549 1106 44-45 0.697914570993639 1106 46-47 0.7116028866104855 1106 48-49 0.5206606688328748 1106 50-51 0.5023958247441911 1106 52-53 0.48759590920182205 1106 54-55 0.5455292635356841 1106 56-57 0.6633894409486913 1106 58-59 0.6161630762056092 1106 60-61 0.5649339597454244 1106 62-63 0.7368914615211537 1106 64-65 0.9233576833774322 1106 66-67 0.8694436016658642 1106 68-69 0.8962215627379138 1106 70-71 0.705586492524418 1106 72-73 0.7638955058297583 1106 74-75 0.9080358430004232 1106 76-77 0.8442408356389954 1106 78-79 0.7167279126848349 1106 80-81 0.6908138434085807 1106 82-83 0.8768661092989056 1106 84-85 0.8707752236240367 1106 86-87 0.6580334530461371 1106 88-89 1.084794454106845 1106 90-91 1.1813033910920296 1106 92-93 1.2701726976346457 1106 94-95 1.1431847234744374 1106 96-97 1.2526683189315229 1106 98-99 1.314053366570338 1106 100-101 1.2154649654442515 1107 1 0.2318805979502656 1107 2 0.10656950620168715 1107 3 0.13274526054416214 1107 4 0.20522327500062687 1107 5 0.18386850604043303 1107 6 0.22135176322730388 1107 7 0.2505313365589501 1107 8 0.18790665108310378 1107 9 0.2104297215117512 1107 10-11 0.3019870937302471 1107 12-13 0.23076934580718245 1107 14-15 0.33744569980483874 1107 16-17 0.3278475204025213 1107 18-19 0.40160626185780757 1107 20-21 0.2779018527127377 1107 22-23 0.26503751920040486 1107 24-25 0.3561402692628235 1107 26-27 0.3877279791502346 1107 28-29 0.4558026121034189 1107 30-31 0.33983416303127 1107 32-33 0.3853603514911583 1107 34-35 0.34833519619520104 1107 36-37 0.44305793428943474 1107 38-39 0.4008731423395844 1107 40-41 0.4806052719119194 1107 42-43 0.5009046211879706 1107 44-45 0.5401434681921913 1107 46-47 0.4378439213680352 1107 48-49 0.23232457696340703 1107 50-51 0.3639688033502111 1107 52-53 0.3421397290591912 1107 54-55 0.25889467762261376 1107 56-57 0.3232927900158913 1107 58-59 0.44227210990061394 1107 60-61 0.46591375091401943 1107 62-63 0.33813688950291976 1107 64-65 0.33171420884791303 1107 66-67 0.18593576537987389 1107 68-69 0.03808531148634842 1107 70-71 0.32480858533383383 1107 72-73 0.2968269102311609 1107 74-75 0.3460756476551978 1107 76-77 0.32424083563899586 1107 78-79 0.23424931688189687 1107 80-81 0.4168850749417139 1107 82-83 0.422754051430946 1107 84-85 0.32442895582126496 1107 86-87 0.22217432229152578 1107 88-89 0.402489952584542 1107 90-91 0.8653425010043705 1107 92-93 0.8062147091183327 1107 94-95 0.8553991128162721 1107 96-97 0.7945633310895133 1107 98-99 0.8185360899606771 1107 100-101 0.9284719741694012 1108 1 0.04728688565163708 1108 2 -0.18788014251025942 1108 3 -0.1456004920638847 1108 4 0.03402179888111334 1108 5 0.05454908229477695 1108 6 0.07921848666779141 1108 7 0.0479105147578025 1108 8 0.08372383935930827 1108 9 0.03773296471663201 1108 10-11 0.03856505129181187 1108 12-13 0.04186720969193658 1108 14-15 0.07968010474416332 1108 16-17 0.12195368745950219 1108 18-19 0.0712620705300111 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-0.6447004776247383 2316 62-63 -0.6240251622817681 2316 64-65 -0.677312990367966 2316 66-67 -0.6443566466106532 2316 68-69 -0.664756312298671 2316 70-71 -0.5561740169695213 2316 72-73 -0.6108844350273515 2316 74-75 -0.707988715654448 2316 76-77 -1.1851392182693594 2316 78-79 -0.7434407917516097 2316 80-81 -0.8250164197899679 2316 82-83 -0.8111077471478332 2316 84-85 -0.6894418274834813 2316 86-87 -0.6628563074560851 2316 88-89 -0.7156510259337949 2316 90-91 -0.7991049383487301 2316 92-93 -1.0418143687220685 2316 94-95 -0.8450435891358623 2316 96-97 -0.6390722157535791 2316 98-99 -0.7395999877980408 2316 100-101 -0.8760898162414463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 14.0 5 37.0 6 87.0 7 198.0 8 478.0 9 925.0 10 1643.0 11 2872.0 12 4476.0 13 6321.0 14 8188.0 15 11072.0 16 15291.0 17 21518.0 18 18881.0 19 10354.0 20 8071.0 21 8041.0 22 8295.0 23 9126.0 24 10399.0 25 12462.0 26 15283.0 27 18790.0 28 23479.0 29 29662.0 30 37300.0 31 46658.0 32 59687.0 33 77956.0 34 106812.0 35 152627.0 36 233676.0 37 361722.0 38 297443.0 39 27258.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.915982781580006 20.073158246606685 15.400393844943135 21.61046512687017 2 15.24959215381038 21.489350463581626 40.360288925708545 22.900768456899446 3 18.72276250299013 26.526544454873637 30.908707863463107 23.841985178673124 4 12.515428529103382 17.95993105504122 38.329960761600155 31.194679654255236 5 12.851719429514045 38.522798127846315 34.180343843403534 14.445138599236106 6 29.65478259760902 37.924130004911646 17.958638995523664 14.46244840195567 7 26.74614719581911 34.47937966574222 21.326904302803936 17.447568835634737 8 26.167785695334807 34.566905852846766 20.299882278872182 18.96542617294624 9 26.9280856868958 16.423281219928445 20.91920833604009 35.72942475713567 10-11 24.094695471435905 27.566804068819874 27.703801473063606 20.634698986680622 12-13 25.688740399040494 25.27823126814931 28.300450306326084 20.73257802648411 14-15 22.76395122860423 25.88453251234129 27.62864478636256 23.722871472691917 16-17 20.813174323801707 29.276012028318636 28.10462912987755 21.8061845180021 18-19 20.74872822933217 27.112195702419932 31.759751759593907 20.37932430865399 20-21 21.86103116330309 26.11783327054551 31.907558915572785 20.113576650578615 22-23 21.44616064593018 26.37930730921315 31.567840214970072 20.606691829886596 24-25 21.05079257817635 26.448557021094842 31.909585646923322 20.591064753805487 26-27 21.028480393034826 26.603496307557524 31.728716356000813 20.639306943406837 28-29 20.815269608773196 26.579167457215497 31.96256972640686 20.642993207604448 30-31 21.076124402538525 26.533376931360735 31.76489733362844 20.6256013324723 32-33 20.857834722289258 26.57126872343248 31.689019583200906 20.881876971077357 34-35 21.2108195470211 26.620805555547122 31.570004878346424 20.598370019085355 36-37 21.30997696197375 26.385233806064683 31.462646006413248 20.84214322554832 38-39 21.128124052299842 26.651111861935018 31.40980748045252 20.810956605312615 40-41 21.31049401867468 26.58998581093511 31.361294149575937 20.73822602081427 42-43 21.180891273865495 26.705126893552457 31.089853828268932 21.024128004313113 44-45 21.33038189406146 26.935394564769965 30.683089005807762 21.05113453536081 46-47 21.46837705534114 27.159584266027004 30.06412439720213 21.307914281429728 48-49 21.573574321083594 27.375615471947835 29.68544905926137 21.365361147707198 50-51 21.7531655406554 27.566188577340217 29.335892155481837 21.344753726522544 52-53 21.804669907516182 27.694479809653217 29.182611367015582 21.31823891581502 54-55 21.59415177552299 27.595861484435336 29.315193580820175 21.4947931592215 56-57 21.61678020917888 27.917494127582483 28.932303730115894 21.533421933122742 58-59 21.679070824976453 27.606019201679562 29.167282649973846 21.54762732337014 60-61 21.772478902313157 27.602270657519277 29.269595045838138 21.35565539432943 62-63 21.75427551115194 27.52425000591945 29.402111451976882 21.31936303095173 64-65 21.675762412520864 27.485099632264326 29.261426246139443 21.577711709075366 66-67 21.641691141315484 27.809363355569218 28.943529965318092 21.605415537797207 68-69 21.78642266802581 27.732736005362106 28.983560933585007 21.497280393027072 70-71 21.669651692965257 27.684155885156425 28.988683208771725 21.657509213106593 72-73 21.700521366937593 27.743004803861304 28.800188208152143 21.756285621048956 74-75 21.723853314888867 27.884748193271047 28.630692672271042 21.760705819569047 76-77 21.630822882142624 27.91853687175225 28.727655438251816 21.72298480785331 78-79 21.697380076242677 27.83744123795086 28.576584791899016 21.888593893907444 80-81 21.645765217681685 28.18896609927657 28.385533829382247 21.779734853659498 82-83 21.781561165538964 28.133698139772513 28.36738392437299 21.71735677031553 84-85 21.716631725429266 27.958545303090187 28.22307870248098 22.101744268999564 86-87 21.535277355398193 27.901469476198383 28.382410484957198 22.180842683446226 88-89 21.86080736862524 27.692116679613733 28.387008250604172 22.060067701156857 90-91 21.53258691508554 27.89639796479957 28.54371336956446 22.027301750550436 92-93 21.5067515100664 27.679521684304596 28.53079904050299 22.28292776512601 94-95 21.343595752560546 27.65392111030836 28.609139644589614 22.393343492541483 96-97 21.455625709776452 27.616174145179468 28.49630453306765 22.431895611976426 98-99 21.2173361864919 27.584152037854036 28.461501332523003 22.737010443131066 100-101 21.27133567624687 27.893360095610788 28.277035713494275 22.558268514648066 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 854.0 1 595.5 2 395.0 3 499.5 4 464.5 5 453.0 6 745.0 7 1520.5 8 2541.0 9 3086.0 10 2812.0 11 2465.5 12 2517.5 13 2643.5 14 2830.5 15 3069.0 16 3292.5 17 3533.5 18 3790.5 19 4027.5 20 4485.0 21 5107.0 22 5783.5 23 6824.5 24 8085.0 25 9736.5 26 12061.5 27 14928.0 28 17369.0 29 19859.5 30 23016.5 31 26309.0 32 30289.5 33 34342.0 34 38593.0 35 42472.5 36 47341.0 37 51898.0 38 53477.5 39 53793.0 40 53836.0 41 54210.5 42 54188.5 43 55732.5 44 58289.0 45 59608.0 46 59642.5 47 59113.5 48 61560.5 49 60077.0 50 55418.0 51 52829.5 52 49686.0 53 47605.5 54 45212.5 55 41965.5 56 38320.5 57 34310.5 58 31411.5 59 28410.5 60 24177.5 61 19631.0 62 15529.0 63 11686.0 64 8018.5 65 5626.0 66 3927.5 67 2779.0 68 1967.5 69 1274.5 70 920.0 71 694.5 72 516.5 73 427.5 74 353.5 75 265.0 76 180.0 77 89.5 78 41.0 79 26.0 80 18.0 81 12.5 82 12.0 83 7.5 84 6.0 85 5.5 86 4.0 87 3.0 88 1.5 89 1.0 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01651374253011178 2 0.0027927652808277274 3 0.0016392317952684484 4 1.2142457742729249E-4 5 0.0015785195065548022 6 3.6427373228187747E-4 7 6.071228871364624E-5 8 0.0 9 0.0 10-11 9.106843307046937E-5 12-13 7.285474645637549E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0019124370944798566 22-23 0.0 24-25 0.0031266828687527815 26-27 0.001973149383193503 28-29 0.003065970580039135 30-31 0.015147716034054735 32-33 7.58903608920578E-4 34-35 0.00233742311547538 36-37 0.0032481074461800743 38-39 0.002033861671907149 40-41 0.004249860209955237 42-43 0.0026409845590436116 44-45 4.249860209955237E-4 46-47 1.2142457742729249E-4 48-49 1.8213686614093873E-4 50-51 6.071228871364624E-5 52-53 8.499720419910474E-4 54-55 0.0029749021469686657 56-57 3.6427373228187747E-4 58-59 0.0010624650524888092 60-61 7.892597532774011E-4 62-63 0.0 64-65 3.6427373228187747E-4 66-67 3.035614435682312E-5 68-69 0.0 70-71 1.8213686614093873E-4 72-73 3.035614435682312E-5 74-75 3.9462987663870056E-4 76-77 7.285474645637549E-4 78-79 2.4284915485458497E-4 80-81 0.0059498042939373314 82-83 0.001791012517052564 84-85 0.003764161900246067 86-87 0.001791012517052564 88-89 0.001305314207343394 90-91 1.517807217841156E-4 92-93 0.0 94-95 1.8213686614093873E-4 96-97 0.0016695879396252716 98-99 0.003005258291325489 100-101 0.0011231773412024556 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1647113.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.91160627656654 #Duplication Level Percentage of deduplicated Percentage of total 1 75.92124624669209 43.208000744179955 2 13.928005204690908 15.853302968546771 3 4.553560105909426 7.774512597125936 4 2.0204585930653782 4.599501757865696 5 1.0300009179480418 2.930950335338054 6 0.5787649204213945 1.9763064766626461 7 0.3872133604660868 1.5425854021110517 8 0.26411809392040114 1.2025107977372071 9 0.20545754935985422 1.0523627240144633 >10 0.9921841303668605 10.409704272092304 >50 0.07764391848784336 3.0378805141172394 >100 0.03920479910984602 3.8980933609307264 >500 0.0013924037152579442 0.5315439132471357 >1k 4.2843191238705973E-4 0.3704958606325792 >5k 2.1421595619352987E-4 0.9081964178290223 >10k+ 1.0710797809676493E-4 0.7040518575693139 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11550 0.7012269346426141 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9005 0.5467141598663844 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5894 0.3578382296782309 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2276 0.13818116911225883 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 6.071228871364624E-5 0.0 0.0 0.0 3 0.0 6.071228871364624E-5 0.0 0.0 0.0 4 0.0 1.2142457742729249E-4 0.0 0.0 0.0 5 0.0 1.2142457742729249E-4 0.0 0.0 0.0 6 0.0 1.2142457742729249E-4 0.0 0.0 0.0 7 6.071228871364624E-5 1.2142457742729249E-4 0.0 0.0 0.0 8 6.071228871364624E-5 1.2142457742729249E-4 0.0 0.0 0.0 9 6.071228871364624E-5 1.2142457742729249E-4 0.0 0.0 0.0 10-11 6.071228871364624E-5 1.2142457742729249E-4 0.0 0.0 0.0 12-13 6.071228871364624E-5 1.2142457742729249E-4 0.0 0.0 0.0 14-15 6.071228871364624E-5 1.2142457742729249E-4 0.0 0.0 0.0 16-17 6.071228871364624E-5 1.2142457742729249E-4 0.0 3.035614435682312E-5 0.0 18-19 6.071228871364624E-5 1.2142457742729249E-4 0.0 6.071228871364624E-5 0.0 20-21 6.071228871364624E-5 2.4284915485458497E-4 0.0 1.2142457742729249E-4 0.0 22-23 6.071228871364624E-5 2.4284915485458497E-4 0.0 3.035614435682312E-4 0.0 24-25 1.2142457742729249E-4 2.4284915485458497E-4 0.0 6.678351758501086E-4 0.0 26-27 1.2142457742729249E-4 2.4284915485458497E-4 0.0 9.410404750615168E-4 0.0 28-29 1.517807217841156E-4 2.4284915485458497E-4 0.0 0.0016999440839820948 0.0 30-31 1.821368661409387E-4 2.4284915485458497E-4 0.0 0.0035820250341051284 0.0 32-33 1.821368661409387E-4 2.4284915485458497E-4 0.0 0.006253365737505563 0.0 34-35 2.1249301049776185E-4 2.4284915485458497E-4 0.0 0.009258624028831052 0.0 36-37 2.4284915485458497E-4 2.4284915485458497E-4 0.0 0.012597799908081594 0.0 38-39 2.4284915485458497E-4 3.035614435682312E-4 0.0 0.01469237386870239 0.0 40-41 2.4284915485458497E-4 3.035614435682312E-4 0.0 0.0177279883043847 0.0 42-43 2.732052992114081E-4 3.035614435682312E-4 0.0 0.023313518866040155 0.0 44-45 3.642737322818774E-4 3.035614435682312E-4 0.0 0.03026507592375265 0.0 46-47 6.071228871364625E-4 3.035614435682312E-4 0.0 0.04759843435149865 0.0 48-49 6.678351758501086E-4 3.035614435682312E-4 0.0 0.08314547939333852 0.0 50-51 6.678351758501086E-4 3.035614435682312E-4 0.0 0.11031422859269521 0.0 52-53 6.678351758501086E-4 3.339175879250543E-4 0.0 0.13496341781043558 0.0 54-55 6.678351758501086E-4 3.642737322818774E-4 0.0 0.1727871736790372 0.0 56-57 7.285474645637548E-4 3.642737322818774E-4 0.0 0.19880238939283462 0.0 58-59 7.285474645637548E-4 3.642737322818774E-4 0.0 0.2279746441197416 0.0 60-61 7.285474645637548E-4 3.642737322818774E-4 0.0 0.2787604736287067 0.0 62-63 7.285474645637548E-4 3.642737322818774E-4 0.0 0.31112012351308016 0.0 64-65 7.285474645637548E-4 4.249860209955237E-4 0.0 0.34997598828981374 0.0 66-67 7.892597532774011E-4 4.249860209955237E-4 0.0 0.4296001549377608 0.0 68-69 7.892597532774011E-4 4.249860209955237E-4 0.0 0.5216096284832916 0.0 70-71 7.892597532774011E-4 4.249860209955237E-4 0.0 0.5983499614173404 0.0 72-73 8.196158976342242E-4 4.249860209955237E-4 0.0 0.6918772421807126 0.0 74-75 8.499720419910474E-4 4.249860209955237E-4 0.0 0.7714103403955892 0.0 76-77 8.499720419910474E-4 4.249860209955237E-4 0.0 0.8313030132116012 0.0 78-79 8.499720419910474E-4 4.249860209955237E-4 0.0 0.9073755109697998 0.0 80-81 0.0010928211968456324 4.249860209955237E-4 0.0 1.0397283003655486 0.0 82-83 0.0010928211968456324 4.249860209955237E-4 0.0 1.1665259153439989 0.0 84-85 0.0011231773412024556 4.249860209955237E-4 0.0 1.3149978173932206 0.0 86-87 0.0011535334855592785 4.249860209955237E-4 0.0 1.5987670548408033 0.0 88-89 0.0011535334855592785 4.249860209955237E-4 0.0 1.9366309415322447 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5930 0.0 48.309578 1 GTATCAA 10475 0.0 39.730198 1 ATCAACG 13170 0.0 31.414047 3 TCAACGC 13360 0.0 30.966352 4 TATCAAC 13500 0.0 30.857258 2 CAACGCA 13660 0.0 30.28627 5 AACGCAG 14075 0.0 29.393284 6 ACGCAGA 15865 0.0 25.837414 7 CGCAGAG 15935 0.0 25.604683 8 GCAGAGT 16670 0.0 24.048342 9 GTACATG 9465 0.0 23.139338 1 GAGTACT 9810 0.0 23.119728 12-13 TACATGG 9630 0.0 22.196156 2 GTACTTT 10295 0.0 21.86907 14-15 ACATGGG 9395 0.0 21.689623 3 AGTACTT 10230 0.0 21.032984 12-13 CAGAGTA 16370 0.0 20.542955 10-11 AGAGTAC 15950 0.0 19.833158 10-11 CATGGGA 6350 0.0 19.448135 4 ATATCCG 125 0.006033454 18.999332 9 >>END_MODULE