FastQCFastQC Report
Thu 26 May 2016
SRR936826_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936826_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505873
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95920.6369727061976674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68840.4571434642894852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45760.30387688736035506No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16560.10996943301327536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47350.047.1679081
GTATCAA86800.042.099351
ATCAACG109800.033.151993
TCAACGC110700.032.8835564
CAACGCA111450.032.5802355
TATCAAC111800.032.5164262
AACGCAG114200.031.8757426
ACGCAGA130100.027.7636937
CGCAGAG130700.027.4926138
GCAGAGT135800.025.7601179
GTACATG85800.025.1995531
TACATGG87650.024.3974132
GAGTACT73050.023.64927912-13
ACATGGG88250.023.3699723
GTACTTT78450.021.9889314-15
AGTACTT79000.021.92825912-13
CAGAGTA132400.020.76616710-11
AGAGTAC127450.020.7150110-11
ACTACGC1150.003714400820.661193
CATGGGA59650.020.1561554
Produced by FastQC (version 0.11.5)