##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936826_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1505873 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88281681124504 33.0 31.0 34.0 30.0 34.0 2 31.99740549169817 33.0 31.0 34.0 30.0 34.0 3 31.997257404840912 33.0 31.0 34.0 30.0 34.0 4 35.597697813826265 37.0 35.0 37.0 33.0 37.0 5 35.6124527101555 37.0 35.0 37.0 33.0 37.0 6 35.688892755232345 37.0 35.0 37.0 33.0 37.0 7 35.63283822739368 37.0 35.0 37.0 33.0 37.0 8 35.58248935999251 37.0 35.0 37.0 33.0 37.0 9 37.23197839392831 39.0 37.0 39.0 34.0 39.0 10-11 37.05969693327392 39.0 37.0 39.0 33.0 39.0 12-13 37.00420819019931 39.0 37.0 39.0 33.0 39.0 14-15 38.15663671504835 40.0 38.0 41.0 33.0 41.0 16-17 38.09993771055062 40.0 38.0 41.0 33.0 41.0 18-19 38.10957464540502 40.0 38.0 41.0 33.0 41.0 20-21 38.05779903086116 40.0 38.0 41.0 33.0 41.0 22-23 37.959125371130234 40.0 38.0 41.0 33.0 41.0 24-25 37.810797789720645 40.0 37.5 41.0 33.0 41.0 26-27 37.88609398003683 40.0 38.0 41.0 33.0 41.0 28-29 37.83592673485745 40.0 38.0 41.0 33.0 41.0 30-31 37.66465067107253 40.0 37.0 41.0 33.0 41.0 32-33 37.443174158777 40.0 36.5 41.0 32.0 41.0 34-35 37.27480869900715 40.0 36.0 41.0 31.5 41.0 36-37 37.09279168960464 40.0 36.0 41.0 31.0 41.0 38-39 36.92404638372558 39.0 36.0 41.0 31.0 41.0 40-41 36.74143271046098 39.0 36.0 41.0 30.5 41.0 42-43 36.503460783213455 39.0 35.0 41.0 30.0 41.0 44-45 36.204678282962774 39.0 35.0 41.0 30.0 41.0 46-47 35.79031598282192 39.0 35.0 40.5 28.5 41.0 48-49 35.415440744338994 39.0 35.0 40.0 26.5 41.0 50-51 34.74519497992195 38.0 34.0 39.5 25.5 40.5 52-53 34.961986834215104 38.5 35.0 40.0 26.5 40.5 54-55 35.34312090063372 39.0 35.0 40.5 27.0 41.0 56-57 35.24982883682754 39.0 35.0 41.0 26.0 41.0 58-59 35.025388263153665 39.0 35.0 41.0 26.0 41.0 60-61 34.76379515404022 38.0 34.5 40.0 26.0 41.0 62-63 34.446639590456826 38.0 34.0 40.0 24.5 41.0 64-65 34.15023577685502 37.5 34.0 40.0 23.5 41.0 66-67 33.79548076099379 37.0 34.0 40.0 23.0 41.0 68-69 33.42964712163642 36.5 33.5 39.0 22.0 41.0 70-71 33.036122900138324 36.0 33.0 39.0 21.5 41.0 72-73 32.64541232892813 35.5 33.0 39.0 20.5 40.0 74-75 32.21143150850038 35.0 32.0 37.5 19.5 40.0 76-77 31.788629585629067 35.0 32.0 37.0 18.0 39.0 78-79 31.34919146568137 35.0 31.5 36.5 15.0 39.0 80-81 30.929906107619964 35.0 31.0 36.0 11.0 38.5 82-83 30.525650569470336 35.0 31.0 36.0 7.5 37.0 84-85 30.170208908719395 34.0 31.0 35.0 6.5 37.0 86-87 29.845783475764556 34.0 30.0 35.0 2.0 36.5 88-89 29.50772176670941 34.0 30.0 35.0 2.0 36.0 90-91 29.168547746058266 34.0 30.0 35.0 2.0 36.0 92-93 28.829542066296426 34.0 29.0 35.0 2.0 35.0 94-95 28.43673404065283 34.0 29.0 35.0 2.0 35.0 96-97 28.001159792359648 34.0 29.0 35.0 2.0 35.0 98-99 27.44558770892366 34.0 28.0 35.0 2.0 35.0 100-101 25.295478436760604 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4864748250180462 1101 2 0.482622160689715 1101 3 0.43196063243649263 1101 4 0.34397739576627373 1101 5 0.3194843133437075 1101 6 0.26506921702193154 1101 7 0.23885240049048662 1101 8 0.246170517031139 1101 9 0.3197711319012626 1101 10-11 0.46548500154880657 1101 12-13 0.47173522725253036 1101 14-15 0.640630140692835 1101 16-17 0.8033584502732367 1101 18-19 0.7424484305294854 1101 20-21 0.7357635525051975 1101 22-23 0.6893532562880651 1101 24-25 0.7100957993641259 1101 26-27 0.8399968629050107 1101 28-29 0.8270739691613471 1101 30-31 0.8276829970403057 1101 32-33 0.9295002543762863 1101 34-35 0.8528840391299397 1101 36-37 0.6319342536002281 1101 38-39 0.7637650012837938 1101 40-41 0.7775724518374787 1101 42-43 0.8008570146458425 1101 44-45 0.9834932473764866 1101 46-47 0.9331436657665506 1101 48-49 1.0178341731795015 1101 50-51 1.0640299988315007 1101 52-53 1.001832691158043 1101 54-55 1.027851526609659 1101 56-57 0.9961633390948492 1101 58-59 0.907099181729258 1101 60-61 1.0091076348391326 1101 62-63 1.0736709842607368 1101 64-65 1.0589533694937643 1101 66-67 1.1166787504291662 1101 68-69 1.0313675351334624 1101 70-71 1.0777788312321803 1101 72-73 1.0619237413930165 1101 74-75 0.9720324725488751 1101 76-77 0.9834155084119836 1101 78-79 1.0127489714408249 1101 80-81 0.748917625615082 1101 82-83 0.7922298356506516 1101 84-85 0.6361591107748943 1101 86-87 0.621860491399076 1101 88-89 0.681238126488374 1101 90-91 0.7468759228951392 1101 92-93 1.0975749856723027 1101 94-95 1.109478222580087 1101 96-97 1.0167713936739808 1101 98-99 0.9872461549539011 1101 100-101 0.9873094931111588 1102 1 0.1284478525772279 1102 2 0.12672785219105975 1102 3 0.17565866879673564 1102 4 0.13880834606995762 1102 5 0.15479396871366902 1102 6 0.0950249821023661 1102 7 0.09517528260431618 1102 8 0.17309616461097477 1102 9 0.07294990176379201 1102 10-11 0.3234507491874581 1102 12-13 0.32076215377475137 1102 14-15 0.3866479775185354 1102 16-17 0.49041662039509504 1102 18-19 0.3919105633537825 1102 20-21 0.22266873350830707 1102 22-23 0.38414597753372703 1102 24-25 0.3948396355207251 1102 26-27 0.37147989525283975 1102 28-29 0.26921070017965576 1102 30-31 0.2762386748025065 1102 32-33 0.29338239517438325 1102 34-35 0.2380658512820375 1102 36-37 0.15830465053374354 1102 38-39 0.24674833283140174 1102 40-41 0.10045073911737035 1102 42-43 0.26243662911910093 1102 44-45 0.372147016018161 1102 46-47 0.35822345844771064 1102 48-49 0.49253302368384055 1102 50-51 0.5017900675781988 1102 52-53 0.34728853532050863 1102 54-55 0.3379675403210953 1102 56-57 0.36271495415411437 1102 58-59 0.4193047833613335 1102 60-61 0.2687224798128369 1102 62-63 0.13900550127358002 1102 64-65 0.2844895056110772 1102 66-67 0.09856531982129013 1102 68-69 0.026393202174034514 1102 70-71 0.1690364032460252 1102 72-73 0.16345323170382642 1102 74-75 0.10797467487789447 1102 76-77 0.07731576883605484 1102 78-79 0.2547423708240544 1102 80-81 0.28688994034263615 1102 82-83 0.22118045711883383 1102 84-85 0.3260106624956407 1102 86-87 0.19882334762355924 1102 88-89 0.09259973693390933 1102 90-91 0.11420997634876429 1102 92-93 0.2823614605304101 1102 94-95 0.22115718158352138 1102 96-97 0.23476339509089783 1102 98-99 0.22950194634642074 1102 100-101 0.06004346478593803 1103 1 0.32698598768707754 1103 2 0.32024243091387916 1103 3 0.2831596384300994 1103 4 0.2387811107798683 1103 5 0.25400314714902095 1103 6 0.12348514728225979 1103 7 0.15262035572607857 1103 8 0.2617370523388374 1103 9 0.2446491557164734 1103 10-11 0.4197553053235339 1103 12-13 0.3647482167210683 1103 14-15 0.4154179262990709 1103 16-17 0.4153766230188083 1103 18-19 0.3476036658383208 1103 20-21 0.5014329785631801 1103 22-23 0.6285720116191982 1103 24-25 0.5417640440443705 1103 26-27 0.49333429054384226 1103 28-29 0.6107551407582434 1103 30-31 0.4866794159446286 1103 32-33 0.6345286444043765 1103 34-35 0.589988220723761 1103 36-37 0.4466376083718018 1103 38-39 0.6144001517495141 1103 40-41 0.6462038875739395 1103 42-43 0.5433394148187105 1103 44-45 0.5075695052381235 1103 46-47 0.5938119260847685 1103 48-49 0.6349190489855729 1103 50-51 0.6162068045908242 1103 52-53 0.6518083101374188 1103 54-55 0.6105190021859883 1103 56-57 0.6562782284348714 1103 58-59 0.6275050297622826 1103 60-61 0.7556395590489942 1103 62-63 0.6975103979220734 1103 64-65 0.5532504852829945 1103 66-67 0.6519978219316585 1103 68-69 0.7057094252809648 1103 70-71 0.5926631599935348 1103 72-73 0.4405320258221437 1103 74-75 0.45005241380696503 1103 76-77 0.5452070016393229 1103 78-79 0.5746579271654539 1103 80-81 0.6003528005329102 1103 82-83 0.7990199143523 1103 84-85 0.5735370279675678 1103 86-87 0.5879603881884563 1103 88-89 0.5729824854452339 1103 90-91 0.7434432102471469 1103 92-93 0.9320015554404044 1103 94-95 0.8054653766965707 1103 96-97 0.8941682683541146 1103 98-99 1.0947261170247096 1103 100-101 1.1328668517723166 1104 1 0.3183680371619424 1104 2 0.289862963791208 1104 3 0.3314083183686307 1104 4 0.3667465552101916 1104 5 0.27106501383725856 1104 6 0.17301994837759338 1104 7 0.27599988639656914 1104 8 0.26913567912828995 1104 9 0.3567215077944894 1104 10-11 0.4883826147089678 1104 12-13 0.36941482898694744 1104 14-15 0.5410283103012006 1104 16-17 0.6575225100307946 1104 18-19 0.5430570722901606 1104 20-21 0.5498934243799951 1104 22-23 0.44965529969877593 1104 24-25 0.6441709898207506 1104 26-27 0.4808904725910139 1104 28-29 0.5221931339759536 1104 30-31 0.6002106598064856 1104 32-33 0.5526953043311309 1104 34-35 0.5516419876650289 1104 36-37 0.6229364029852604 1104 38-39 0.6231912339688463 1104 40-41 0.5523499002372887 1104 42-43 0.46959795044593733 1104 44-45 0.5951486872621743 1104 46-47 0.5348743950329933 1104 48-49 0.6456295641272831 1104 50-51 0.5276471447449538 1104 52-53 0.5277951066342013 1104 54-55 0.6433113004794393 1104 56-57 0.5856533150909797 1104 58-59 0.6507476437728457 1104 60-61 0.6553387179069787 1104 62-63 0.6994083473495607 1104 64-65 0.6844225669939306 1104 66-67 0.6044987173781777 1104 68-69 0.6203551389807203 1104 70-71 0.6600128332999873 1104 72-73 0.6389812063441624 1104 74-75 0.6180286108167508 1104 76-77 0.4894273638041078 1104 78-79 0.4286319043344555 1104 80-81 0.5160286387863842 1104 82-83 0.41636854056404005 1104 84-85 0.35776059640856417 1104 86-87 0.3190081544826917 1104 88-89 0.476179301699613 1104 90-91 0.5673087011829168 1104 92-93 0.6212195128980298 1104 94-95 0.6576278800183886 1104 96-97 0.8222249183442933 1104 98-99 0.9381914155839048 1104 100-101 0.9798204199822358 1105 1 0.17950142139453007 1105 2 0.31448519473472203 1105 3 0.3641026292683307 1105 4 0.34023421026213185 1105 5 0.2910085735594805 1105 6 0.2635228773400584 1105 7 0.22727982964804028 1105 8 0.250351073558015 1105 9 0.27222423940018103 1105 10-11 0.30185828550583693 1105 12-13 0.17599748557451278 1105 14-15 0.41808932587284175 1105 16-17 0.18357040870744612 1105 18-19 0.21498508255421456 1105 20-21 0.2996352629256194 1105 22-23 0.3892445795796249 1105 24-25 0.39051190127823787 1105 26-27 0.2914625553665715 1105 28-29 0.3342961414300305 1105 30-31 0.059092785936634584 1105 32-33 0.16318126437032987 1105 34-35 0.23259202181714755 1105 36-37 0.17594887555736705 1105 38-39 0.15426627953946337 1105 40-41 0.2970593290416801 1105 42-43 0.15826407052831115 1105 44-45 0.08662400244094215 1105 46-47 0.14431116887755024 1105 48-49 0.3339246534772755 1105 50-51 0.0915505158617691 1105 52-53 0.062178791506937614 1105 54-55 0.05860126268843402 1105 56-57 0.4057141235319861 1105 58-59 0.22485755533069351 1105 60-61 0.2576759107869506 1105 62-63 0.18839151638808005 1105 64-65 0.20672538596289058 1105 66-67 0.3439607115386707 1105 68-69 0.3448326792161325 1105 70-71 0.22151127117413694 1105 72-73 0.23508436867631133 1105 74-75 0.17374543504930529 1105 76-77 0.1763965915602128 1105 78-79 0.1134503191874181 1105 80-81 0.17116271545930672 1105 82-83 0.34373748908850743 1105 84-85 0.29704726257150327 1105 86-87 0.18740953079243639 1105 88-89 0.19741530393556772 1105 90-91 0.09857828414362402 1105 92-93 0.09439572016700026 1105 94-95 0.15938736263539255 1105 96-97 0.19623893330656728 1105 98-99 0.2350340455489821 1105 100-101 0.43409398353506035 1106 1 0.05716088830506294 1106 2 0.13178818768300005 1106 3 0.17264771845167814 1106 4 0.14416628204527626 1106 5 0.07027467991870395 1106 6 0.06274998422539824 1106 7 0.20067991457547407 1106 8 0.18983130694441996 1106 9 -0.01051050046280011 1106 10-11 0.22014843594492106 1106 12-13 0.1820555284815626 1106 14-15 0.15563015380473644 1106 16-17 0.32010309527979786 1106 18-19 0.24375587422979805 1106 20-21 0.2689591359678687 1106 22-23 0.11158545604148884 1106 24-25 0.22915300882971934 1106 26-27 0.15026992383300808 1106 28-29 0.22355720784550215 1106 30-31 0.25254993935656245 1106 32-33 0.2832047968253164 1106 34-35 0.21298793782215597 1106 36-37 0.10842049678251442 1106 38-39 0.1643518899086942 1106 40-41 0.2315232732230612 1106 42-43 0.3443789682739222 1106 44-45 0.4877401916482853 1106 46-47 0.33141574217820846 1106 48-49 0.2847819589730207 1106 50-51 0.2837637073899373 1106 52-53 0.31807859696739627 1106 54-55 0.2993362169684062 1106 56-57 0.35959634908873994 1106 58-59 0.34052407358418435 1106 60-61 0.37021983860854135 1106 62-63 0.2518144966239504 1106 64-65 0.17540314661020062 1106 66-67 0.36180234132142175 1106 68-69 0.3950869464766029 1106 70-71 0.29524324460017226 1106 72-73 0.3097781663406849 1106 74-75 0.41888394956689723 1106 76-77 0.47602601643815134 1106 78-79 0.1681689999048821 1106 80-81 0.32913023319353485 1106 82-83 0.3185463546585936 1106 84-85 0.3121346642000802 1106 86-87 0.3521063844711563 1106 88-89 0.5264345446171106 1106 90-91 0.44961379991376305 1106 92-93 0.5754297365443364 1106 94-95 0.3914359490666115 1106 96-97 0.5963784188049956 1106 98-99 0.5067680876029463 1106 100-101 0.6520204263000338 1107 1 0.08584362166543968 1107 2 0.04902248334394699 1107 3 0.039806816289896574 1107 4 0.03912755450377858 1107 5 0.13213989648502178 1107 6 -0.01092797518103339 1107 7 -0.009966021655408497 1107 8 0.09436434759138734 1107 9 0.18017799800086465 1107 10-11 0.18076303185935672 1107 12-13 0.19888082827867493 1107 14-15 0.1397700600072369 1107 16-17 0.12957366549175475 1107 18-19 0.09690140048011386 1107 20-21 0.21859609958924153 1107 22-23 0.13571143994752788 1107 24-25 0.15509195902948392 1107 26-27 0.1116986105052149 1107 28-29 0.1883530375193061 1107 30-31 0.022490996837547073 1107 32-33 0.05942092419235934 1107 34-35 0.10372290706319376 1107 36-37 0.19532236542283954 1107 38-39 -0.03844343907751835 1107 40-41 0.18594934018567244 1107 42-43 0.03926631167175998 1107 44-45 0.0934871535312638 1107 46-47 0.16555994702230237 1107 48-49 0.2995202398724004 1107 50-51 0.27646056150388176 1107 52-53 0.3056610144249632 1107 54-55 0.37517315491154335 1107 56-57 0.3862272859009792 1107 58-59 0.3911802508078992 1107 60-61 0.44180150011116126 1107 62-63 0.4427921592158057 1107 64-65 0.5082113639464865 1107 66-67 0.4313316381734893 1107 68-69 0.394061521621218 1107 70-71 0.11317437468085245 1107 72-73 0.27633397728984477 1107 74-75 0.38279069896442053 1107 76-77 0.43997840339618577 1107 78-79 0.47029040793534804 1107 80-81 0.29273380527001436 1107 82-83 0.3369242783503239 1107 84-85 0.42162655718927766 1107 86-87 0.38680115630040035 1107 88-89 0.33218972504833033 1107 90-91 0.32911473330602803 1107 92-93 0.46332305751576186 1107 94-95 0.4975228255600115 1107 96-97 0.4449929495770206 1107 98-99 0.4228877663296693 1107 100-101 0.3883985613258609 1108 1 0.17718192382641362 1108 2 0.19645941204595374 1108 3 0.16372712754476026 1108 4 0.19359134300047742 1108 5 0.28889647080649183 1108 6 0.14216366687587367 1108 7 0.157396843389364 1108 8 0.26347398992955107 1108 9 0.18546483060762142 1108 10-11 0.13933875960509567 1108 12-13 0.3167048567791184 1108 14-15 0.5286989495211358 1108 16-17 0.482977312423742 1108 18-19 0.47729796917649736 1108 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808.0 5 1026.0 6 998.0 7 1245.0 8 1562.0 9 1857.0 10 2567.0 11 3484.0 12 4875.0 13 6367.0 14 7418.0 15 9587.0 16 12789.0 17 17646.0 18 15433.0 19 8792.0 20 7640.0 21 7887.0 22 8230.0 23 9293.0 24 10581.0 25 12165.0 26 13935.0 27 16906.0 28 20292.0 29 24917.0 30 30553.0 31 38851.0 32 49603.0 33 65856.0 34 90200.0 35 131466.0 36 201162.0 37 308922.0 38 300552.0 39 57175.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80513159835297 19.56974931938759 14.8398086180346 21.785310464224832 2 14.955306872876204 21.019288962408613 41.0905056584721 22.934898506243087 3 18.628473352312202 26.69484714393697 30.446273548123127 24.230405955627702 4 12.202374822864895 17.467032773565364 38.90116110275862 31.429431300811117 5 12.86178568984226 38.4416959980225 34.25765035074698 14.438867961388263 6 29.524924612830905 37.61450942190102 18.466168471218648 14.39439749404943 7 26.80590413652369 34.37020528124254 21.51628900313706 17.307601579096705 8 25.91679502838134 34.553201556023076 20.580757571126124 18.94924584446946 9 26.951985726294385 16.60441302043705 21.019692796363792 35.423908456904776 10-11 23.974743176999453 27.52070649459739 27.942362396135252 20.562187932267907 12-13 25.567888058501342 25.333868071606197 28.292527974077373 20.805715895815087 14-15 22.80410709165401 26.000350198573223 27.47374258278425 23.721800126988516 16-17 20.973916902984378 29.352254696897617 27.857805394688235 21.81602300542977 18-19 20.894277398004302 27.510518976013483 31.119227020530353 20.475976605451862 20-21 22.152537519110876 26.546992657303857 31.068255646221708 20.232214177363552 22-23 21.620701509069864 26.709789274925726 30.82554607670067 20.84396313930374 24-25 21.222043704574027 26.883008802418157 31.13680245003849 20.75814504296932 26-27 21.084595211995282 27.047301894818776 30.950975935114105 20.917126958071833 28-29 20.929573788435437 27.02454893331708 31.17388753046491 20.871989747782564 30-31 21.185425313097944 26.985864849014963 31.02958864406025 20.79912119382684 32-33 20.923367399424674 27.05519742825749 30.862072343374514 21.159362828943326 34-35 21.302492726903832 27.12850758158636 30.703108578252074 20.86589111325774 36-37 21.480106044426027 26.833729140434485 30.640085164937336 21.046079650202152 38-39 21.142954484777565 27.194770936245217 30.65158081950512 21.010693759472097 40-41 21.348706733671545 27.01809915384978 30.712244506266266 20.920949606212407 42-43 21.177172578351882 27.166833908552444 30.39959410361116 21.25639940948451 44-45 21.310724243278482 27.390992954498593 30.049317513743855 21.248965288479067 46-47 21.516422632382664 27.44848617061581 29.569570606577688 21.465520590423843 48-49 21.55184979690933 27.649982119786475 29.27118788701033 21.526980196293866 50-51 21.702444311327934 27.87883637870555 28.957511777933327 21.46120753203319 52-53 21.75108422541263 27.99776995394802 28.81089335658124 21.44025246405811 54-55 21.55344712567686 27.947550546918503 28.877018132769983 21.62198419463465 56-57 21.61919722622598 28.280671922844448 28.560706196488944 21.539424654440626 58-59 21.666646189259723 27.89593979509532 28.777409147556543 21.660004868088418 60-61 21.704340722020103 27.96198111343397 28.906309414888405 21.42736874965752 62-63 21.71180571237291 27.891154881233543 29.00297148595551 21.394067920438033 64-65 21.598005191575925 27.840016842569458 28.929271502418963 21.632706463435653 66-67 21.571842213367223 28.180235923447917 28.587112712375767 21.660809150809087 68-69 21.624250805904676 28.121805359324213 28.710997568170598 21.542946266600506 70-71 21.545455511556327 27.96347193311291 28.710658071098088 21.780414484232676 72-73 21.62780935630721 28.04989260052843 28.462752906826143 21.859545136338216 74-75 21.527679070762193 28.29046386171955 28.370233890240158 21.811623177278094 76-77 21.58856311608258 28.196953795467227 28.436611579031272 21.777871509418922 78-79 21.573024302959425 28.251314263734955 28.277621128664155 21.898040304641466 80-81 21.486698293033133 28.578849526982886 28.132353786179593 21.802098393804396 82-83 21.679667649573304 28.53336557284813 28.073122142368902 21.71384463520967 84-85 21.683417102115996 28.308313401300406 27.98321258982699 22.02505690675661 86-87 21.530113191405132 28.29971112644744 28.162879781707513 22.007295900439917 88-89 21.83371032496557 28.00812588276983 28.189088508292098 21.969075283972504 90-91 21.595592730755474 28.236949502309205 28.307286745813226 21.860171021122095 92-93 21.615274166221877 28.1390920567839 28.263943299799422 21.981690477194803 94-95 21.345322993159506 28.09332592503784 28.390432671247368 22.17091841055529 96-97 21.511136236963658 28.01421101685964 28.272097925279226 22.202554820897475 98-99 21.253817956638244 28.113980913705046 28.21972427658982 22.41247685306689 100-101 21.414261223187943 28.232796427717087 28.18508625043192 22.16785609866305 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 452.0 1 344.0 2 277.0 3 345.5 4 338.5 5 356.0 6 581.0 7 1234.5 8 2167.0 9 2624.5 10 2338.5 11 2111.0 12 2218.0 13 2351.5 14 2571.0 15 2660.0 16 2731.0 17 2952.0 18 3147.5 19 3365.5 20 3665.5 21 4200.0 22 4980.5 23 6006.5 24 7154.0 25 8669.5 26 11027.0 27 13666.0 28 15746.0 29 18164.5 30 21282.0 31 24548.5 32 28300.0 33 31995.5 34 36117.0 35 39605.5 36 43777.0 37 48039.0 38 49240.5 39 49460.5 40 49481.5 41 49878.0 42 50211.5 43 51409.0 44 53496.0 45 54622.5 46 54416.0 47 53678.0 48 56429.5 49 55604.5 50 50654.0 51 48058.0 52 45302.0 53 43184.0 54 41205.5 55 38298.5 56 34760.5 57 30930.0 58 28471.0 59 25864.0 60 21953.0 61 17831.5 62 14205.5 63 10615.5 64 7275.0 65 5194.0 66 3590.5 67 2519.0 68 1731.5 69 1153.0 70 841.5 71 626.5 72 458.0 73 388.5 74 296.5 75 228.0 76 172.0 77 82.0 78 49.0 79 31.0 80 19.0 81 13.5 82 11.5 83 9.0 84 6.5 85 7.0 86 5.5 87 3.5 88 2.0 89 1.5 90 2.0 91 2.5 92 1.0 93 0.5 94 0.5 95 1.5 96 2.0 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04057447075550196 2 0.040375250768159066 3 0.022511858569746586 4 0.04422683719012161 5 0.0625550760256675 6 0.0647464958864393 7 0.0638832092746201 8 0.06713713573455397 9 0.06693791574721109 10-11 0.0724828720615882 12-13 0.07218404208057386 14-15 0.06723674572822542 16-17 0.05558237646866635 18-19 0.04887530356145572 20-21 0.05578159645600924 22-23 0.03157636799384809 24-25 0.017531358886174332 26-27 0.014244229095016644 28-29 0.016435648955788435 30-31 0.01288289251484023 32-33 0.00820122281228231 34-35 0.010127016023263581 36-37 0.0059101929578390736 38-39 0.008964899430430056 40-41 0.007437546194134566 42-43 0.004648466371334104 44-45 0.0012285232552811558 46-47 0.0026230631666813867 48-49 0.0012285232552811558 50-51 0.006043006282734334 52-53 0.004947296352348438 54-55 0.007869189500044161 56-57 0.022777485219537104 58-59 0.009861389373473061 60-61 0.02028723537775098 62-63 0.01520712570050728 64-65 0.010923895972635142 66-67 0.017099715580264737 68-69 0.028023611552899882 70-71 0.019324338772260342 72-73 0.01852745882288878 74-75 0.019589965422050864 76-77 0.026097818341918606 78-79 0.0373537476267919 80-81 0.03210762129342913 82-83 0.031476758000176644 84-85 0.022578265232194215 86-87 0.024570465105623118 88-89 0.02081848867733202 90-91 0.017896595529636294 92-93 0.03197480796853387 94-95 0.02347475517523722 96-97 0.03732054429556809 98-99 0.05405502323237085 100-101 0.06328554931259144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1505873.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.57762154478685 #Duplication Level Percentage of deduplicated Percentage of total 1 77.5983302960693 46.23123954886585 2 13.25578607800624 15.7949641246822 3 4.224704657137482 7.550935656043063 4 1.7580885840870963 4.1897094521980485 5 0.8901240411047057 2.651573662442623 6 0.5358565517968731 1.9155035307149146 7 0.31142362905361826 1.298771537830239 8 0.23592496971500898 1.1244678846916882 9 0.17239878046955795 0.9243998367838303 >10 0.9119650514705863 10.060130938795309 >50 0.06999317504006627 2.838208156134711 >100 0.033840661177211045 3.424599646956805 >500 8.934427840787916E-4 0.3425298766574704 >1k 4.467213920393958E-4 0.5567108543110052 >5k 2.233606960196979E-4 1.0962552928920906 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9592 0.6369727061976674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6884 0.4571434642894852 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4576 0.30387688736035506 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1656 0.10996943301327536 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 6.640666244763005E-5 0.0 0.0 0.0 0.0 16-17 9.960999367144508E-5 0.0 0.0 0.0 0.0 18-19 1.6601665611907512E-4 0.0 0.0 0.0 0.0 20-21 1.9921998734289014E-4 0.0 0.0 0.0 0.0 22-23 1.9921998734289014E-4 0.0 0.0 9.960999367144508E-5 0.0 24-25 1.9921998734289014E-4 0.0 0.0 3.9843997468578027E-4 0.0 26-27 1.9921998734289014E-4 0.0 0.0 5.976599620286704E-4 0.0 28-29 4.316433059095953E-4 0.0 0.0 0.0011621165928335257 0.0 30-31 4.648466371334103E-4 6.640666244763005E-5 0.0 0.003054706472590982 0.0 32-33 4.980499683572253E-4 6.640666244763005E-5 0.0 0.005578159645600924 0.0 34-35 5.312532995810404E-4 6.640666244763005E-5 0.0 0.007902392831267976 0.0 36-37 6.640666244763005E-4 6.640666244763005E-5 0.0 0.010060609360815952 0.0 38-39 6.640666244763005E-4 6.640666244763005E-5 0.0 0.012285232552811559 0.0 40-41 6.640666244763005E-4 6.640666244763005E-5 0.0 0.015339939025402542 0.0 42-43 6.640666244763005E-4 6.640666244763005E-5 0.0 0.021150521989570167 0.0 44-45 7.968799493715605E-4 6.640666244763005E-5 0.0 0.02795720489045225 0.0 46-47 8.632866118191906E-4 6.640666244763005E-5 0.0 0.04704912034414589 0.0 48-49 8.632866118191906E-4 6.640666244763005E-5 0.0 0.08978180762919583 0.0 50-51 8.632866118191906E-4 6.640666244763005E-5 0.0 0.11568040598377154 0.0 52-53 8.632866118191906E-4 6.640666244763005E-5 0.0 0.14310635757464274 0.0 54-55 8.632866118191906E-4 1.328133248952601E-4 0.0 0.18354801500524945 0.0 56-57 8.632866118191906E-4 1.328133248952601E-4 0.0 0.20901497005391556 0.0 58-59 8.632866118191906E-4 1.9921998734289014E-4 0.0 0.2391303914739158 0.0 60-61 8.632866118191906E-4 1.9921998734289014E-4 0.0 0.29225572143201983 0.0 62-63 8.632866118191906E-4 1.9921998734289014E-4 0.0 0.3296094690588117 0.0 64-65 8.632866118191906E-4 2.656266497905202E-4 0.0 0.36766048664130374 0.0 66-67 9.960999367144507E-4 2.656266497905202E-4 0.0 0.45292664122406073 0.0 68-69 9.960999367144507E-4 2.656266497905202E-4 0.0 0.5553589180495301 0.0 70-71 0.0010293032679382658 2.656266497905202E-4 0.0 0.6352129296428052 0.0 72-73 0.0010625065991620808 2.656266497905202E-4 0.0 0.7323990801349118 0.0 74-75 0.0010625065991620808 2.656266497905202E-4 0.0 0.8192921979476357 0.0 76-77 0.0010625065991620808 2.656266497905202E-4 0.0 0.8855328437391466 0.0 78-79 0.0010625065991620808 2.656266497905202E-4 0.0 0.9659845152944504 0.0 80-81 0.0011621165928335257 2.656266497905202E-4 0.0 1.1063681997087405 0.0 82-83 0.001328133248952601 2.988299810143352E-4 0.0 1.237654171367705 0.0 84-85 0.001460946573847861 3.3203331223815024E-4 0.0 1.3904559016597018 0.0 86-87 0.001593759898743121 3.3203331223815024E-4 0.0 1.6930710624335519 0.0 88-89 0.001593759898743121 3.3203331223815024E-4 0.0 2.051102583019949 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4735 0.0 47.167908 1 GTATCAA 8680 0.0 42.09935 1 ATCAACG 10980 0.0 33.15199 3 TCAACGC 11070 0.0 32.883556 4 CAACGCA 11145 0.0 32.580235 5 TATCAAC 11180 0.0 32.516426 2 AACGCAG 11420 0.0 31.875742 6 ACGCAGA 13010 0.0 27.763693 7 CGCAGAG 13070 0.0 27.492613 8 GCAGAGT 13580 0.0 25.760117 9 GTACATG 8580 0.0 25.199553 1 TACATGG 8765 0.0 24.397413 2 GAGTACT 7305 0.0 23.649279 12-13 ACATGGG 8825 0.0 23.369972 3 GTACTTT 7845 0.0 21.98893 14-15 AGTACTT 7900 0.0 21.928259 12-13 CAGAGTA 13240 0.0 20.766167 10-11 AGAGTAC 12745 0.0 20.71501 10-11 ACTACGC 115 0.0037144008 20.66119 3 CATGGGA 5965 0.0 20.156155 4 >>END_MODULE