FastQCFastQC Report
Thu 26 May 2016
SRR936826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936826_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505873
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110130.7313365735357497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85610.5685074372141609No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58140.38608833547052107No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20860.1385242978657563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51700.049.6203541
GTATCAA94850.041.3212281
ATCAACG123300.031.547293
TCAACGC123950.031.3818534
TATCAAC125200.031.0685372
CAACGCA125350.031.0313595
AACGCAG129500.029.9992476
ACGCAGA147600.026.1917727
CGCAGAG147700.026.0775728
GTACATG89950.024.875761
GCAGAGT155800.024.4169759
TACATGG90850.024.2568442
ACATGGG90150.023.1807883
GAGTACT91800.022.712412-13
AGTACTT96200.021.79700912-13
GTACTTT96300.021.67645314-15
AGAGTAC147100.020.66303610-11
CATGGGG29900.020.4908394
CAGAGTA152450.020.40519310-11
AGGGTCG1200.00476749819.7892785
Produced by FastQC (version 0.11.5)