##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936826_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1505873 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25028936703161 33.0 31.0 34.0 31.0 34.0 2 32.3135855414102 33.0 31.0 34.0 31.0 34.0 3 32.441112895974626 34.0 31.0 34.0 31.0 34.0 4 36.11663400565652 37.0 35.0 37.0 35.0 37.0 5 36.04302886099956 37.0 35.0 37.0 35.0 37.0 6 36.08560084416149 37.0 35.0 37.0 35.0 37.0 7 36.03791886832422 37.0 35.0 37.0 35.0 37.0 8 36.0253892592536 37.0 35.0 37.0 35.0 37.0 9 37.72439110071035 39.0 37.0 39.0 35.0 39.0 10-11 37.6300063152736 39.0 37.0 39.0 35.0 39.0 12-13 37.56025607737173 39.0 37.0 39.0 35.0 39.0 14-15 38.775681614585025 40.0 38.0 41.0 35.0 41.0 16-17 38.707160896038374 40.0 38.0 41.0 35.0 41.0 18-19 38.77956507620496 40.0 38.0 41.0 35.0 41.0 20-21 38.8807890838072 40.0 38.0 41.0 35.0 41.0 22-23 38.81734117020493 40.0 38.0 41.0 35.0 41.0 24-25 38.73181138117225 40.0 38.0 41.0 35.0 41.0 26-27 38.561017761790005 40.0 38.0 41.0 34.0 41.0 28-29 38.40519021192358 40.0 38.0 41.0 34.0 41.0 30-31 38.304573825282745 40.0 38.0 41.0 34.0 41.0 32-33 38.173112208001605 40.0 37.0 41.0 34.0 41.0 34-35 38.04518508532924 40.0 37.0 41.0 33.5 41.0 36-37 37.85529921845999 40.0 37.0 41.0 33.0 41.0 38-39 37.70441431647954 40.0 37.0 41.0 33.0 41.0 40-41 37.455304331773 40.0 37.0 41.0 33.0 41.0 42-43 37.19321250862457 40.0 36.5 41.0 32.5 41.0 44-45 36.87862190237822 40.0 36.0 41.0 31.0 41.0 46-47 36.43238041986277 40.0 36.0 41.0 30.5 41.0 48-49 35.99417812790322 39.0 36.0 41.0 29.5 41.0 50-51 35.7271217426702 39.0 35.0 41.0 28.0 41.0 52-53 35.49833352480587 39.0 35.0 40.5 27.5 41.0 54-55 35.24412317639004 39.0 35.0 40.0 27.0 41.0 56-57 35.01153815760028 38.5 35.0 40.0 26.0 41.0 58-59 34.68875662157433 38.0 34.0 40.0 26.0 41.0 60-61 34.3834450182718 38.0 34.0 40.0 25.5 41.0 62-63 34.061804348706694 37.5 34.0 40.0 24.5 41.0 64-65 33.66525928813386 37.0 33.5 39.5 23.0 41.0 66-67 33.25301735272497 36.0 33.0 39.0 22.0 40.5 68-69 32.8326993710625 36.0 32.5 39.0 21.5 40.0 70-71 32.457218503818055 35.0 32.0 38.5 20.0 40.0 72-73 32.079288890895846 35.0 32.0 38.0 19.0 40.0 74-75 31.611696338270228 35.0 31.0 37.0 18.0 39.0 76-77 30.145991062991367 33.5 29.5 35.5 14.0 38.5 78-79 30.8345335230793 34.0 31.0 36.0 14.5 38.5 80-81 30.54045294656322 34.0 31.0 36.0 9.0 37.5 82-83 30.338837670905846 34.0 31.0 35.5 7.5 37.0 84-85 30.013831843721217 34.0 30.5 35.0 7.0 37.0 86-87 29.65568875994191 34.0 30.0 35.0 2.0 36.0 88-89 29.30111536630247 34.0 30.0 35.0 2.0 36.0 90-91 28.87794090205482 34.0 29.0 35.0 2.0 35.5 92-93 28.47305217637875 34.0 29.0 35.0 2.0 35.0 94-95 28.03024889881152 34.0 29.0 35.0 2.0 35.0 96-97 27.455934863032937 33.0 27.0 35.0 2.0 35.0 98-99 26.733948679603127 33.0 26.0 35.0 2.0 35.0 100-101 23.581241246771803 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2486716973558032 1101 2 0.28390930567564254 1101 3 0.21360878677811002 1101 4 0.17707732019123767 1101 5 0.22777540150179476 1101 6 0.23748143597346427 1101 7 0.19520311029198467 1101 8 0.20038573031153817 1101 9 0.30014996547911466 1101 10-11 0.3712299474478087 1101 12-13 0.30489380911303243 1101 14-15 0.44215200648851294 1101 16-17 0.3516025088608572 1101 18-19 0.3377200052802536 1101 20-21 0.3220245051654942 1101 22-23 0.3408711069343937 1101 24-25 0.3486295434101834 1101 26-27 0.34866684231479184 1101 28-29 0.3878995989397751 1101 30-31 0.37256116310216214 1101 32-33 0.39564692356582754 1101 34-35 0.49742183439319376 1101 36-37 0.5001338345030248 1101 38-39 0.46489753851714966 1101 40-41 0.5989880607327862 1101 42-43 0.4746482023225411 1101 44-45 0.554899850271795 1101 46-47 0.39593428505095574 1101 48-49 0.5694035914699285 1101 50-51 0.46522002323918343 1101 52-53 0.44842174551453695 1101 54-55 0.3773932160756672 1101 56-57 0.4434117440794765 1101 58-59 0.5251695132663841 1101 60-61 0.5576582023906838 1101 62-63 0.5289484849701509 1101 64-65 0.6646332189661663 1101 66-67 0.6470273680986764 1101 68-69 0.47238062353407884 1101 70-71 0.6035948650878069 1101 72-73 0.49666602054698217 1101 74-75 0.5488882881247328 1101 76-77 0.4381480360061438 1101 78-79 0.6282600887999443 1101 80-81 0.96286741080743 1101 82-83 0.9418197611391292 1101 84-85 0.8662653610020463 1101 86-87 0.7141808272484163 1101 88-89 0.6603907164513778 1101 90-91 0.5319496221243512 1101 92-93 0.8466866599671725 1101 94-95 0.8474045782029727 1101 96-97 0.8571676292542492 1101 98-99 1.0217647366755962 1101 100-101 1.1765762950396663 1102 1 0.10022086706731415 1102 2 0.12303712921410437 1102 3 0.10915162118033095 1102 4 0.07094048130066 1102 5 0.16024152924443058 1102 6 0.181618906062603 1102 7 0.0979365790487492 1102 8 0.10492569516559058 1102 9 0.11639137487406259 1102 10-11 0.1644338850772229 1102 12-13 0.16900991611940697 1102 14-15 0.20698590141363127 1102 16-17 0.2731226988326725 1102 18-19 0.1037273755802417 1102 20-21 0.18595548003197848 1102 22-23 0.1681047371351312 1102 24-25 0.15780258583824036 1102 26-27 0.1778922829693741 1102 28-29 -0.010609905117455298 1102 30-31 0.13091652643127816 1102 32-33 0.2156598536609593 1102 34-35 0.20067323582162544 1102 36-37 0.21642283889298852 1102 38-39 0.07303384096183407 1102 40-41 0.09521125574909917 1102 42-43 -0.08349914245254553 1102 44-45 0.0918446451796342 1102 46-47 0.04138546446659319 1102 48-49 0.23067026702876348 1102 50-51 0.21451284033081208 1102 52-53 0.17730993955051133 1102 54-55 0.03487670906724816 1102 56-57 0.11852652236187566 1102 58-59 0.27963418619111025 1102 60-61 0.2828732727299652 1102 62-63 0.2981408204995617 1102 64-65 0.23081686567574167 1102 66-67 0.08013198306390734 1102 68-69 0.07934436168726222 1102 70-71 0.11911225475378728 1102 72-73 0.12346349691894432 1102 74-75 0.1931435795147074 1102 76-77 0.19733722283143962 1102 78-79 0.15978266216584203 1102 80-81 0.19775709318919255 1102 82-83 0.17388636060428908 1102 84-85 0.25128828021519567 1102 86-87 0.30718439942407727 1102 88-89 0.2950659662522135 1102 90-91 0.3644839353460654 1102 92-93 0.34160190370383603 1102 94-95 0.4010848812365886 1102 96-97 0.39497904665944006 1102 98-99 0.6201065285068985 1102 100-101 0.7733382683150403 1103 1 0.2161120171665587 1103 2 0.1892224489649621 1103 3 0.128770298600422 1103 4 0.1324266440621713 1103 5 0.1755275140497048 1103 6 0.23284406633729304 1103 7 0.2321744757428732 1103 8 0.14837667734715154 1103 9 0.2409115489517717 1103 10-11 0.16503954944719368 1103 12-13 0.2993304227243243 1103 14-15 0.34860192374942045 1103 16-17 0.3215680058071868 1103 18-19 0.4450172874006384 1103 20-21 0.28990801933065313 1103 22-23 0.25006406671921155 1103 24-25 0.1547885233025923 1103 26-27 0.2339335266097251 1103 28-29 0.3055864656018912 1103 30-31 0.43030508703907344 1103 32-33 0.36750408206095386 1103 34-35 0.47984173243361283 1103 36-37 0.48516278607648644 1103 38-39 0.4089747275489515 1103 40-41 0.47199901726491333 1103 42-43 0.5255917407123363 1103 44-45 0.6362149966432185 1103 46-47 0.6652949834143698 1103 48-49 0.5931420335866804 1103 50-51 0.600158487817751 1103 52-53 0.5487165923761381 1103 54-55 0.5256626967243889 1103 56-57 0.4175657297721571 1103 58-59 0.5124656154306777 1103 60-61 0.403859632270013 1103 62-63 0.44032436353987947 1103 64-65 0.45952628366756443 1103 66-67 0.3721292281644111 1103 68-69 0.40133500472529704 1103 70-71 0.4979187786752135 1103 72-73 0.429360365003177 1103 74-75 0.45989827368093117 1103 76-77 0.45349475117896176 1103 78-79 0.4853805552095807 1103 80-81 0.8118911392981119 1103 82-83 0.6989528090083859 1103 84-85 0.5929596286900178 1103 86-87 0.6932335855024228 1103 88-89 0.6263864785608568 1103 90-91 0.5665091857396227 1103 92-93 0.4989480884817361 1103 94-95 0.55924099506014 1103 96-97 0.6717922610510811 1103 98-99 0.8554533007685379 1103 100-101 1.025203743093165 1104 1 0.11872932065937647 1104 2 0.07519564045186655 1104 3 0.05854743351821412 1104 4 0.11739469684152226 1104 5 -0.01915988846216976 1104 6 0.1782882655770308 1104 7 0.07691163330679274 1104 8 0.0961403719016829 1104 9 0.07683650186861257 1104 10-11 0.14207405446747146 1104 12-13 0.11940073693939013 1104 14-15 0.13895749544869318 1104 16-17 0.14217865699900045 1104 18-19 0.14082851138936547 1104 20-21 0.2004063353135095 1104 22-23 0.07471740615698508 1104 24-25 0.18416873711210968 1104 26-27 0.27371409888669973 1104 28-29 0.2226206384403966 1104 30-31 0.3531262634824728 1104 32-33 0.12226493285067619 1104 34-35 0.12964758663181186 1104 36-37 0.1425456342318654 1104 38-39 0.2704448196355145 1104 40-41 0.17082912362085523 1104 42-43 0.10464594700010821 1104 44-45 0.2257233604024691 1104 46-47 0.2711311643638936 1104 48-49 0.18589007226997722 1104 50-51 0.3107911568838304 1104 52-53 0.020878315792934643 1104 54-55 0.03261919679948733 1104 56-57 0.09290787699512038 1104 58-59 0.11157475447330256 1104 60-61 0.21836229116844663 1104 62-63 0.21925432662437316 1104 64-65 0.059080437961675614 1104 66-67 -0.11558979516341594 1104 68-69 0.21755742641705922 1104 70-71 0.09922374753678298 1104 72-73 0.09290590712382851 1104 74-75 0.2929855999354274 1104 76-77 0.31722602623701945 1104 78-79 0.37450586283878096 1104 80-81 0.37838436134573783 1104 82-83 0.37259649639934267 1104 84-85 0.0071135689435131155 1104 86-87 0.1783603272903136 1104 88-89 0.21839126053407298 1104 90-91 0.3071860710878056 1104 92-93 0.24703389103288842 1104 94-95 0.24286909157194358 1104 96-97 0.07027751218050327 1104 98-99 0.378787445299384 1104 100-101 0.5629390505490584 1105 1 0.15952127391626902 1105 2 0.1344338733832302 1105 3 0.13832768708971344 1105 4 0.08665252636060927 1105 5 0.1140822472463654 1105 6 0.09605740476693114 1105 7 0.11400418085192143 1105 8 -0.006701678398357558 1105 9 0.0468533091472807 1105 10-11 0.13688733052598678 1105 12-13 0.18395350581731407 1105 14-15 0.2596545069408691 1105 16-17 0.29492133346509064 1105 18-19 0.1623714128045819 1105 20-21 0.28375010036195647 1105 22-23 0.08425142990069645 1105 24-25 0.24656279881598664 1105 26-27 0.21038155873963404 1105 28-29 0.1651811715217164 1105 30-31 0.0944264950101612 1105 32-33 0.12358327609131692 1105 34-35 0.05915719828254851 1105 36-37 0.11827507426115602 1105 38-39 0.1405541778425743 1105 40-41 0.12662402595180566 1105 42-43 0.054418460426951754 1105 44-45 0.20170165792400496 1105 46-47 0.2086767678752608 1105 48-49 0.4029889126767685 1105 50-51 0.21812482177654147 1105 52-53 0.10157486866556553 1105 54-55 0.13176165342892432 1105 56-57 0.07161251736032881 1105 58-59 0.27090764347196483 1105 60-61 0.17124589916600996 1105 62-63 0.1615235235176371 1105 64-65 0.1295236486443443 1105 66-67 -0.06682763563674143 1105 68-69 0.023644059649605254 1105 70-71 -0.022252541268727555 1105 72-73 0.189016023932723 1105 74-75 0.009348393560014046 1105 76-77 -0.03595049680278706 1105 78-79 0.12078925889397851 1105 80-81 0.2240983549182971 1105 82-83 -0.07013301167104657 1105 84-85 -0.2028153938959001 1105 86-87 -0.18700551389875741 1105 88-89 -0.17630837561522483 1105 90-91 -0.09354422415416508 1105 92-93 -4.3496136943232955E-4 1105 94-95 -0.03121830251795643 1105 96-97 0.13693466813031918 1105 98-99 0.08434542552385693 1105 100-101 0.3885258486215015 1106 1 0.14651089376869209 1106 2 0.06089762222386952 1106 3 0.11807024172693303 1106 4 0.010822929109721713 1106 5 0.05064038152862338 1106 6 0.019976001091094986 1106 7 -0.04953798775841989 1106 8 0.043823280768940265 1106 9 0.06499329139111154 1106 10-11 0.1301442596855935 1106 12-13 0.08549070773669598 1106 14-15 0.11266326942293148 1106 16-17 0.20271745114414585 1106 18-19 0.15888961669224244 1106 20-21 0.13831929962897505 1106 22-23 0.18670345219243956 1106 24-25 0.12285087452273302 1106 26-27 0.23051195631526866 1106 28-29 0.1835197374688633 1106 30-31 0.06006750832499819 1106 32-33 0.22971396133672073 1106 34-35 0.14395892552201417 1106 36-37 0.21729985849929534 1106 38-39 0.31547208322515985 1106 40-41 0.3190201586629442 1106 42-43 0.22961583278875253 1106 44-45 0.23642061333939068 1106 46-47 0.24476120085870434 1106 48-49 0.28683728553034626 1106 50-51 0.15558869613887083 1106 52-53 0.17577459160219178 1106 54-55 0.3057816399916149 1106 56-57 0.25596515165613454 1106 58-59 0.4803253182227607 1106 60-61 0.36223077132274994 1106 62-63 0.491621716577626 1106 64-65 0.4751848141036774 1106 66-67 0.37235386376369206 1106 68-69 0.5710010269235397 1106 70-71 0.41879537787546184 1106 72-73 0.35603846255585836 1106 74-75 0.5690763052721017 1106 76-77 0.40737684277458186 1106 78-79 0.2577711910199376 1106 80-81 0.7251889438057759 1106 82-83 0.6648660974683445 1106 84-85 0.39596025459148976 1106 86-87 0.26500685819025094 1106 88-89 0.16817731166942806 1106 90-91 0.2340114870635439 1106 92-93 0.2909987366635427 1106 94-95 0.45080558017687977 1106 96-97 0.5945205465207835 1106 98-99 0.7626430691117037 1106 100-101 0.7741170271556932 1107 1 0.06516430925720584 1107 2 -0.0637429109743266 1107 3 -0.08583042518742445 1107 4 -0.028151926298740193 1107 5 -0.008863043939555837 1107 6 0.006385855168311139 1107 7 -0.03695035322703433 1107 8 0.021222273106751288 1107 9 0.10772330374923911 1107 10-11 -0.04171732481263035 1107 12-13 -0.013940470253956505 1107 14-15 0.02425252077378559 1107 16-17 0.07293085692190004 1107 18-19 0.04138501790320959 1107 20-21 0.07302425942815205 1107 22-23 0.018921032386714387 1107 24-25 -0.12130344633166601 1107 26-27 -0.09902361238744106 1107 28-29 -0.06035450889594074 1107 30-31 -0.04154537229888433 1107 32-33 0.04774015388783681 1107 34-35 0.02949992376885291 1107 36-37 0.10511326639796437 1107 38-39 0.014310578905124771 1107 40-41 0.1202625228930998 1107 42-43 -0.0662003959277655 1107 44-45 -0.11659065137398272 1107 46-47 0.10140320809329495 1107 48-49 0.07540376998763776 1107 50-51 0.048394398675142725 1107 52-53 -0.009840373578818173 1107 54-55 -0.041424754266152775 1107 56-57 -0.015026137818942686 1107 58-59 -0.00823423538508905 1107 60-61 -0.017771039030272107 1107 62-63 0.05416411122019582 1107 64-65 -0.03994697240040779 1107 66-67 -0.061273595125577174 1107 68-69 -0.1460807521762746 1107 70-71 0.02443383219756612 1107 72-73 -0.08633038816159555 1107 74-75 -0.03724595498773198 1107 76-77 0.16558014693396572 1107 78-79 0.1944989741441887 1107 80-81 0.20633305661727874 1107 82-83 0.18820450206255046 1107 84-85 0.08538813380079802 1107 86-87 0.1593852575730672 1107 88-89 0.11597286600293799 1107 90-91 -0.04651331837084882 1107 92-93 -0.09528571827438626 1107 94-95 -0.017352279576815732 1107 96-97 0.07901059317714498 1107 98-99 0.07579664726687696 1107 100-101 0.5560762911828014 1108 1 0.054052510635351325 1108 2 0.08873297146133297 1108 3 0.06711608960186766 1108 4 0.08652824057886477 1108 5 0.020288036546077137 1108 6 0.05896017664208131 1108 7 0.0989716455541938 1108 8 0.12194997197704538 1108 9 0.13118404532332306 1108 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-0.5981343664512799 2316 76-77 -0.6548588100844093 2316 78-79 -0.4423512195689163 2316 80-81 -0.33790959407194876 2316 82-83 -0.5116385925432816 2316 84-85 -0.9847177745602238 2316 86-87 -0.7764008484598399 2316 88-89 -0.6849507741132506 2316 90-91 -0.7329316052441719 2316 92-93 -0.8459718318689973 2316 94-95 -0.7661223903780474 2316 96-97 -1.0877105622514947 2316 98-99 -1.007145256592704 2316 100-101 -1.4626372625075312 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 18.0 5 29.0 6 68.0 7 176.0 8 417.0 9 813.0 10 1442.0 11 2506.0 12 3984.0 13 5771.0 14 7110.0 15 10245.0 16 14331.0 17 20230.0 18 17839.0 19 9654.0 20 7376.0 21 7139.0 22 7284.0 23 8051.0 24 9081.0 25 10588.0 26 12994.0 27 16215.0 28 20397.0 29 25426.0 30 31714.0 31 40415.0 32 52334.0 33 69025.0 34 95769.0 35 138562.0 36 215298.0 37 341122.0 38 278778.0 39 23663.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.866129200463114 20.02598543035712 15.488233156798026 21.619652212381737 2 15.300548625514107 21.4877108814601 40.345060942525485 22.86667955050031 3 18.76920992580353 26.499337199448508 30.916391495963424 23.815061378784538 4 12.47583624360918 17.961166660358025 38.3175584097177 31.245438686315097 5 12.822321217482315 38.522814511394635 34.141477331590735 14.513386939532314 6 29.634172337242248 37.90664949833087 17.939494233577467 14.519683930849414 7 26.686048557879715 34.49367908183492 21.338452844296963 17.481819515988402 8 26.139339864211564 34.53653431367341 20.311088857485895 19.013036964629134 9 26.965421386796894 16.34918748128162 20.934833149940268 35.75055798198122 10-11 24.068961990818615 27.579483794450127 27.65113658323112 20.700417631500134 12-13 25.72940369042358 25.348959289660034 28.28430465966363 20.637332360252756 14-15 22.73574863218877 25.902483144328905 27.70100798672929 23.660760236753035 16-17 20.78270238682226 29.29869560669977 28.185719574532374 21.732882431945598 18-19 20.688654448182568 27.057037891999368 31.901054983178444 20.35325267663962 20-21 21.874339037628825 26.113463228660688 32.02668785509347 19.985509878617012 22-23 21.37318352875707 26.367960644755566 31.71489229171384 20.543963534773514 24-25 21.038180328777916 26.41997863577787 32.08866982315246 20.453171212291764 26-27 20.952702570329855 26.618837618655323 31.84015322296525 20.58830658804958 28-29 20.716957374122696 26.519440120285413 32.12487846604222 20.638724039549675 30-31 20.961579659722165 26.529182685521167 31.9790087269889 20.53022892776777 32-33 20.816388465359424 26.573415660378362 31.780559200565257 20.82963667369696 34-35 21.10493612559117 26.660131962506473 31.71410138022382 20.520830531678538 36-37 21.213608563351244 26.3997919801438 31.600720099387264 20.78587935711769 38-39 21.021923163002846 26.628929330656497 31.64347681136224 20.705670694978412 40-41 21.24142648653226 26.564335180170385 31.52665405925043 20.66758427404693 42-43 21.09198180871524 26.6671138336645 31.252590026670635 20.988314330949624 44-45 21.2465791359334 26.972645705068228 30.793952583055358 20.986822575943016 46-47 21.39562836812554 27.161095597488455 30.18776832940973 21.255507704976274 48-49 21.507764875885833 27.3488454870882 29.802507086522144 21.340882550503817 50-51 21.69365225726997 27.60406273066529 29.37500767846666 21.327277333598083 52-53 21.729899663508505 27.73860049553968 29.215457490970113 21.316042349981704 54-55 21.523067212228046 27.652032955484596 29.335833361833302 21.489066470454052 56-57 21.592831497899382 27.91261902208398 29.03141083981272 21.463138640203923 58-59 21.628950652086285 27.673075633243215 29.181151754579943 21.516821960090553 60-61 21.707906310824352 27.638353300709955 29.392053645958192 21.2616867425075 62-63 21.70352347110281 27.555178955994297 29.462577521477574 21.27872005142532 64-65 21.672387417646256 27.484874073193517 29.339732250923195 21.503006258237033 66-67 21.501963160647136 27.929376022047165 28.982094978791224 21.586565838514474 68-69 21.709372705832195 27.753275295487985 29.07202645139162 21.465325547288206 70-71 21.613669605813435 27.68822354560381 29.067071318166647 21.631035530416103 72-73 21.654887251001835 27.742781925625643 28.888469012455054 21.713861810917468 74-75 21.667083944584604 27.903972065180362 28.67101078436516 21.757933205869872 76-77 21.582663820823445 27.940520194285913 28.802839910693294 21.67397607419735 78-79 21.604068964120287 27.877365241464407 28.670862390207603 21.8477034042077 80-81 21.71933111114136 28.175032315195764 28.395369986994233 21.71026658666864 82-83 21.796857697187523 28.14910149478037 28.349957315232732 21.704083492799374 84-85 21.64219171841729 28.018184963471025 28.247290002818985 22.092333315292695 86-87 21.432219051673016 27.926691029057565 28.488192563383496 22.152897355885923 88-89 21.79004063157748 27.692460430116313 28.457710055161588 22.05978888314462 90-91 21.48110664965314 27.87439473975179 28.623833349769118 22.020665260825954 92-93 21.416713040868178 27.7219221364982 28.64129344842104 22.22007137421258 94-95 21.274231935951722 27.687792523096377 28.608327179612502 22.429648361339403 96-97 21.347434156811065 27.623243581200096 28.606462910630704 22.422859351358134 98-99 21.139082769124414 27.570815399342376 28.51797582725462 22.772126004278594 100-101 21.18295739681239 27.928099629348385 28.30505992749001 22.583883046349214 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 769.0 1 524.0 2 382.5 3 485.5 4 400.0 5 374.5 6 662.5 7 1437.0 8 2405.5 9 2867.5 10 2612.5 11 2265.5 12 2341.5 13 2575.5 14 2790.0 15 2942.0 16 3097.5 17 3324.5 18 3605.5 19 3879.0 20 4187.5 21 4734.5 22 5368.5 23 6296.0 24 7511.0 25 8934.5 26 11005.5 27 13604.0 28 15962.0 29 18342.0 30 21233.5 31 24487.0 32 27997.5 33 31587.0 34 35669.5 35 39143.0 36 43546.5 37 47798.0 38 48998.0 39 49084.0 40 49473.5 41 50032.5 42 50029.5 43 51156.5 44 52934.0 45 53934.5 46 54378.5 47 53997.5 48 56040.0 49 54838.5 50 50386.0 51 47854.5 52 45063.5 53 43341.0 54 41246.5 55 38245.5 56 34662.5 57 30862.0 58 28413.0 59 25696.5 60 21882.0 61 17764.5 62 14037.0 63 10504.0 64 7149.0 65 5063.0 66 3544.5 67 2409.5 68 1697.5 69 1160.5 70 811.0 71 619.5 72 462.5 73 390.5 74 310.0 75 250.0 76 187.5 77 87.0 78 38.5 79 26.0 80 14.0 81 8.5 82 7.0 83 3.5 84 3.0 85 3.0 86 3.0 87 3.0 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02749235825331884 2 0.007304732869239305 3 0.009363339405115837 4 1.328133248952601E-4 5 0.0051133130084675136 6 0.0 7 0.0 8 6.640666244763005E-5 9 0.0 10-11 0.0 12-13 1.6601665611907512E-4 14-15 0.0 16-17 3.3203331223815025E-5 18-19 0.004449246383991213 20-21 0.001294929917728786 22-23 0.0 24-25 0.005611362976824739 26-27 0.00620902293885341 28-29 0.008035206156163236 30-31 0.03224043461832439 32-33 5.976599620286704E-4 34-35 0.004416043052767398 36-37 0.016435648955788435 38-39 0.007570359519029826 40-41 0.009662169386130171 42-43 0.006441446257420115 44-45 0.002656266497905202 46-47 0.0018593865485336415 48-49 0.001427743242624046 50-51 0.0025566565042337568 52-53 0.002656266497905202 54-55 0.0015605565675193061 56-57 4.980499683572253E-4 58-59 9.296932742668207E-4 60-61 0.0 62-63 0.0 64-65 0.0064746495886439295 66-67 6.972699557001154E-4 68-69 0.0010957099303858958 70-71 0.0033535364536053176 72-73 0.009230526080220576 74-75 0.005445346320705664 76-77 0.0033867397848291324 78-79 0.00886528943675861 80-81 1.6601665611907512E-4 82-83 0.004416043052767398 84-85 8.964899430430057E-4 86-87 0.0 88-89 0.0016269632299669363 90-91 8.300832805953756E-4 92-93 4.6484663713341036E-4 94-95 5.976599620286704E-4 96-97 2.3242331856670518E-4 98-99 2.3242331856670518E-4 100-101 7.636766181477456E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1505873.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.912914095683234 #Duplication Level Percentage of deduplicated Percentage of total 1 76.6704326090623 44.40208177367496 2 13.704044686873651 15.872823254286361 3 4.366968368733222 7.58711591991039 4 1.855654963545722 4.298655459802064 5 0.988373138589968 2.861978433482082 6 0.5462486176675347 1.898090956191939 7 0.35960710289548586 1.4578126680728656 8 0.2549398697886684 1.1811448622908658 9 0.1735569660236462 0.9046070697631353 >10 0.9649951004086468 10.294363156385245 >50 0.07615523608488378 3.013308256314354 >100 0.03695748238054545 3.680420437681374 >500 0.0012624687445218611 0.48460787421754664 >1k 4.59079543462495E-4 0.3755362075612589 >5k 2.295397717312475E-4 0.9554571652554152 >10k+ 1.1476988586562375E-4 0.731996505110114 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11013 0.7313365735357497 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8561 0.5685074372141609 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5814 0.38608833547052107 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2086 0.1385242978657563 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.328133248952601E-4 0.0 0.0 0.0 3 0.0 1.328133248952601E-4 0.0 0.0 0.0 4 0.0 1.328133248952601E-4 0.0 0.0 0.0 5 0.0 1.328133248952601E-4 0.0 0.0 0.0 6 0.0 1.328133248952601E-4 0.0 0.0 0.0 7 0.0 1.328133248952601E-4 0.0 0.0 0.0 8 0.0 1.328133248952601E-4 0.0 0.0 0.0 9 0.0 1.328133248952601E-4 0.0 0.0 0.0 10-11 0.0 1.328133248952601E-4 0.0 0.0 0.0 12-13 0.0 1.328133248952601E-4 0.0 0.0 0.0 14-15 0.0 1.328133248952601E-4 0.0 3.3203331223815025E-5 0.0 16-17 0.0 1.328133248952601E-4 0.0 6.640666244763005E-5 0.0 18-19 0.0 1.328133248952601E-4 0.0 6.640666244763005E-5 0.0 20-21 0.0 2.656266497905202E-4 0.0 6.640666244763005E-5 0.0 22-23 0.0 2.656266497905202E-4 0.0 1.6601665611907512E-4 0.0 24-25 0.0 2.656266497905202E-4 0.0 4.648466371334103E-4 0.0 26-27 0.0 2.656266497905202E-4 0.0 6.640666244763005E-4 0.0 28-29 3.3203331223815025E-5 2.656266497905202E-4 0.0 0.0012285232552811558 0.0 30-31 6.640666244763005E-5 2.988299810143352E-4 0.0 0.003054706472590982 0.0 32-33 6.640666244763005E-5 3.3203331223815024E-4 0.0 0.005578159645600924 0.0 34-35 9.960999367144508E-5 3.3203331223815024E-4 0.0 0.007902392831267976 0.0 36-37 1.328133248952601E-4 3.3203331223815024E-4 0.0 0.010093812692039766 0.0 38-39 1.9921998734289014E-4 3.3203331223815024E-4 0.0 0.01235163921525919 0.0 40-41 1.9921998734289014E-4 3.3203331223815024E-4 0.0 0.015439549019073986 0.0 42-43 3.3203331223815024E-4 3.3203331223815024E-4 0.0 0.021382945308136878 0.0 44-45 3.9843997468578027E-4 3.6523664346196525E-4 0.0 0.02842205152758566 0.0 46-47 5.312532995810404E-4 4.316433059095953E-4 0.0 0.04744756031883167 0.0 48-49 5.312532995810404E-4 4.648466371334103E-4 0.0 0.09011384094143397 0.0 50-51 5.312532995810404E-4 4.648466371334103E-4 0.0 0.11614525262090496 0.0 52-53 5.312532995810404E-4 4.648466371334103E-4 0.0 0.14367081420544758 0.0 54-55 5.312532995810404E-4 5.312532995810404E-4 0.0 0.1849757582478735 0.0 56-57 5.976599620286704E-4 5.312532995810404E-4 0.0 0.21100716992734447 0.0 58-59 5.976599620286704E-4 5.312532995810404E-4 0.0 0.24145462465958284 0.0 60-61 5.976599620286704E-4 5.312532995810404E-4 0.0 0.2949451912611488 0.0 62-63 5.976599620286704E-4 5.312532995810404E-4 0.0 0.3323321422191646 0.0 64-65 5.976599620286704E-4 5.312532995810404E-4 0.0 0.3703499564704328 0.0 66-67 6.640666244763005E-4 5.312532995810404E-4 0.0 0.45511806108483255 0.0 68-69 6.640666244763005E-4 5.312532995810404E-4 0.0 0.557517134579078 0.0 70-71 6.640666244763005E-4 5.312532995810404E-4 0.0 0.6362422329107436 0.0 72-73 6.640666244763005E-4 5.312532995810404E-4 0.0 0.7329635367657166 0.0 74-75 6.640666244763005E-4 5.312532995810404E-4 0.0 0.818694537985607 0.0 76-77 6.640666244763005E-4 5.312532995810404E-4 0.0 0.8838062705155083 0.0 78-79 6.640666244763005E-4 5.312532995810404E-4 0.0 0.9635274687838882 0.0 80-81 7.304732869239305E-4 5.312532995810404E-4 0.0 1.102848646599016 0.0 82-83 7.304732869239305E-4 5.312532995810404E-4 0.0 1.2349647015385758 0.0 84-85 7.304732869239305E-4 5.312532995810404E-4 0.0 1.3875672118432298 0.0 86-87 7.304732869239305E-4 5.312532995810404E-4 0.0 1.6900827626234085 0.0 88-89 7.304732869239305E-4 5.312532995810404E-4 0.0 2.0473838099228816 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5170 0.0 49.620354 1 GTATCAA 9485 0.0 41.321228 1 ATCAACG 12330 0.0 31.54729 3 TCAACGC 12395 0.0 31.381853 4 TATCAAC 12520 0.0 31.068537 2 CAACGCA 12535 0.0 31.031359 5 AACGCAG 12950 0.0 29.999247 6 ACGCAGA 14760 0.0 26.191772 7 CGCAGAG 14770 0.0 26.077572 8 GTACATG 8995 0.0 24.87576 1 GCAGAGT 15580 0.0 24.416975 9 TACATGG 9085 0.0 24.256844 2 ACATGGG 9015 0.0 23.180788 3 GAGTACT 9180 0.0 22.7124 12-13 AGTACTT 9620 0.0 21.797009 12-13 GTACTTT 9630 0.0 21.676453 14-15 AGAGTAC 14710 0.0 20.663036 10-11 CATGGGG 2990 0.0 20.490839 4 CAGAGTA 15245 0.0 20.405193 10-11 AGGGTCG 120 0.004767498 19.789278 5 >>END_MODULE