FastQCFastQC Report
Thu 26 May 2016
SRR936825_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936825_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551756
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96060.6190406223658874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71010.45761060372893675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45530.29340953087985483No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.10716891057614727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50550.045.5964051
GTATCAA92250.041.8311351
ATCAACG117050.032.6849983
TCAACGC118850.032.1499944
TATCAAC120100.031.8945162
CAACGCA121200.031.5658345
AACGCAG124400.030.866476
ACGCAGA140850.027.02457
CGCAGAG140800.026.8982288
GCAGAGT146550.025.2891799
GTACATG93650.024.2058641
TACATGG94250.023.4474472
GAGTACT78800.022.85130312-13
ACATGGG93450.022.2750573
AGTACTT81800.021.92611112-13
GTACTTT84250.021.03201514-15
AGAGTAC139250.020.67742210-11
CATGGGA61600.020.3679454
CATGGGG33150.020.214344
CAGAGTA144050.020.07087310-11
Produced by FastQC (version 0.11.5)