FastQCFastQC Report
Thu 26 May 2016
SRR936825_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936825_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551756
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109200.7037188836389225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84650.5455110210625898No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56430.3636525330013224No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21060.13571721327322078No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15520.1000157241215758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55750.047.881851
GTATCAA99000.040.1098021
ATCAACG126150.031.3217163
TCAACGC127500.030.9900784
CAACGCA129850.030.47665
TATCAAC129950.030.4058072
AACGCAG134250.029.4777376
ACGCAGA150300.026.2983067
CGCAGAG151250.026.0074888
GCAGAGT158400.024.5936019
CGTCGAG500.001654125523.75037438-39
GAGTACT92300.023.66822212-13
GTACATG86000.022.5341511
GTACTTT97700.022.23924314-15
TACATGG86400.021.9315172
AGTACTT96800.021.12065112-13
ACATGGG85400.020.90933
CAGAGTA155150.020.57694810-11
AGAGTAC150600.020.34689910-11
GTAGGAC4600.019.6157673
Produced by FastQC (version 0.11.5)