##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936825_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1551756 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.250775250748184 33.0 31.0 34.0 31.0 34.0 2 32.36817644011043 34.0 31.0 34.0 31.0 34.0 3 32.465266446528965 34.0 31.0 34.0 31.0 34.0 4 36.11110638528222 37.0 35.0 37.0 35.0 37.0 5 36.032594041846785 37.0 35.0 37.0 35.0 37.0 6 36.08252006114363 37.0 35.0 37.0 35.0 37.0 7 36.02758552246616 37.0 35.0 37.0 35.0 37.0 8 36.000978891011215 37.0 35.0 37.0 35.0 37.0 9 37.69072843926494 39.0 37.0 39.0 35.0 39.0 10-11 37.61315213216511 39.0 37.0 39.0 35.0 39.0 12-13 37.521995726132204 39.0 37.0 39.0 35.0 39.0 14-15 38.719543536483826 40.0 38.0 41.0 35.0 41.0 16-17 38.64040480591021 40.0 38.0 41.0 34.0 41.0 18-19 38.828037719847714 40.0 38.0 41.0 35.0 41.0 20-21 38.871647024403316 40.0 38.0 41.0 35.0 41.0 22-23 38.791954727418485 40.0 38.0 41.0 35.0 41.0 24-25 38.68966222782448 40.0 38.0 41.0 34.0 41.0 26-27 38.509187011360034 40.0 38.0 41.0 34.0 41.0 28-29 38.42389718486669 40.0 38.0 41.0 34.0 41.0 30-31 38.367917056547554 40.0 38.0 41.0 34.0 41.0 32-33 38.15592238728254 40.0 37.0 41.0 34.0 41.0 34-35 37.993517344221644 40.0 37.0 41.0 33.0 41.0 36-37 37.82059647264132 40.0 37.0 41.0 33.0 41.0 38-39 37.64974744740797 40.0 37.0 41.0 33.0 41.0 40-41 37.44746210100041 40.0 37.0 41.0 33.0 41.0 42-43 37.16487547011257 40.0 36.0 41.0 32.0 41.0 44-45 36.830919294012716 40.0 36.0 41.0 31.0 41.0 46-47 36.38196469032502 39.5 36.0 41.0 30.5 41.0 48-49 35.94297363760798 39.0 35.5 41.0 29.5 41.0 50-51 35.62972948066577 39.0 35.0 40.5 28.0 41.0 52-53 35.38488557479397 39.0 35.0 40.0 27.0 41.0 54-55 35.10963257110009 38.5 35.0 40.0 26.5 41.0 56-57 34.84589007550156 38.0 34.5 40.0 26.0 41.0 58-59 34.515680944684604 38.0 34.0 40.0 25.5 41.0 60-61 34.20351073235741 38.0 34.0 40.0 24.5 41.0 62-63 33.84677520177141 37.0 33.5 40.0 23.5 41.0 64-65 33.46971044416777 37.0 33.0 39.0 22.5 41.0 66-67 33.09102236434079 36.0 33.0 39.0 22.0 40.0 68-69 32.79157354635652 36.0 32.0 39.0 21.5 40.0 70-71 32.3431489873408 35.0 32.0 38.5 19.5 40.0 72-73 31.846100160076713 35.0 31.0 37.5 18.0 39.5 74-75 31.331765109978633 35.0 31.0 37.0 17.0 39.0 76-77 29.918440785793642 33.5 29.5 35.5 13.0 38.0 78-79 30.65412474641632 34.0 30.5 36.0 12.5 38.5 80-81 30.427265626812463 34.0 31.0 36.0 8.0 37.5 82-83 30.096144625830348 34.0 30.0 35.0 7.0 37.0 84-85 29.73858164556799 34.0 30.0 35.0 4.5 36.5 86-87 29.394260437852346 34.0 29.5 35.0 2.0 36.0 88-89 29.003700968451227 34.0 29.0 35.0 2.0 36.0 90-91 28.55472058751505 34.0 29.0 35.0 2.0 35.0 92-93 28.086703386357133 33.5 28.5 35.0 2.0 35.0 94-95 27.537405043060893 33.0 27.0 35.0 2.0 35.0 96-97 26.91426100495181 33.0 26.0 34.5 2.0 35.0 98-99 26.110836046388734 32.5 25.0 34.0 2.0 35.0 100-101 22.566477590549027 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09800485346354293 1101 2 0.04411193812729408 1101 3 0.1899860146075696 1101 4 0.09943234054667727 1101 5 0.1609969335017567 1101 6 0.14311515513738016 1101 7 0.1281858745791311 1101 8 0.15644531513999738 1101 9 0.13165953428699595 1101 10-11 0.2627605098736723 1101 12-13 0.18362562164498542 1101 14-15 0.30019290773067553 1101 16-17 0.2840962894386152 1101 18-19 0.16661005808638407 1101 20-21 0.29248365359051576 1101 22-23 0.21383843443687311 1101 24-25 0.2539891179922833 1101 26-27 0.186029591547495 1101 28-29 0.2879446656194986 1101 30-31 0.2976259013154916 1101 32-33 0.3670557506999472 1101 34-35 0.4039798854414869 1101 36-37 0.41067176269908856 1101 38-39 0.45135992742790876 1101 40-41 0.4454067593427524 1101 42-43 0.5841294332874583 1101 44-45 0.5283962533216666 1101 46-47 0.7660748164175359 1101 48-49 0.7369057784443669 1101 50-51 0.6124301098612719 1101 52-53 0.5772345854734624 1101 54-55 0.5358098222106236 1101 56-57 0.5869720128491167 1101 58-59 0.6093363862495949 1101 60-61 0.8651079372848827 1101 62-63 0.9184793646659699 1101 64-65 0.6801883540778704 1101 66-67 0.6594529891372858 1101 68-69 0.5687427133176044 1101 70-71 0.7171445655250324 1101 72-73 0.7492532957598925 1101 74-75 0.7970734666567552 1101 76-77 0.6838783638426094 1101 78-79 0.7094458326555291 1101 80-81 0.9733467129364151 1101 82-83 0.8572340069902538 1101 84-85 0.5699524929472695 1101 86-87 0.37711041267903767 1101 88-89 0.43644641992394284 1101 90-91 0.8035500395989104 1101 92-93 0.9670447109219857 1101 94-95 0.9016671608860243 1101 96-97 0.8496419557413049 1101 98-99 1.095901494781934 1101 100-101 1.3749510850089983 1102 1 0.2739867629628634 1102 2 0.3846562791248047 1102 3 0.3025623451988295 1102 4 0.16208214121392928 1102 5 0.19021333298626075 1102 6 0.14518375932637895 1102 7 0.18023520792851855 1102 8 0.22747491996734226 1102 9 0.2723878515813709 1102 10-11 0.32607650948825295 1102 12-13 0.28637984617435563 1102 14-15 0.41870029087110083 1102 16-17 0.4803637686923494 1102 18-19 0.293248832220101 1102 20-21 0.3062352042908927 1102 22-23 0.2633584700879297 1102 24-25 0.30548441470299537 1102 26-27 0.2704915737942315 1102 28-29 0.2895375209557116 1102 30-31 0.3359763776665474 1102 32-33 0.3067304950602079 1102 34-35 0.2685683626838298 1102 36-37 0.1352478042277525 1102 38-39 0.2391819612241477 1102 40-41 0.234823154009554 1102 42-43 0.36971047921227296 1102 44-45 0.18847219247398783 1102 46-47 0.25653951464823166 1102 48-49 0.4328563427186438 1102 50-51 0.4475492578492748 1102 52-53 0.33679171749075465 1102 54-55 0.3313115866968772 1102 56-57 0.22179562444534184 1102 58-59 0.3352734308254526 1102 60-61 0.328549799570645 1102 62-63 0.49294309333254915 1102 64-65 0.3179093665196078 1102 66-67 0.3038021165295888 1102 68-69 0.32524436338324136 1102 70-71 0.37950891688062427 1102 72-73 0.607649510244336 1102 74-75 0.6335016767570814 1102 76-77 0.4989528275712729 1102 78-79 0.47164517744698387 1102 80-81 0.6172614936703944 1102 82-83 0.5825590518551778 1102 84-85 0.41055630255137743 1102 86-87 0.355221448847999 1102 88-89 0.649770850530853 1102 90-91 0.5689215452536978 1102 92-93 0.45583073835884136 1102 94-95 0.4902339825223585 1102 96-97 0.8640137865915136 1102 98-99 0.9050697472781088 1102 100-101 0.9762675242396099 1103 1 0.28065341048693426 1103 2 0.2871787226194513 1103 3 0.23489770334000326 1103 4 0.18428802842289826 1103 5 0.21472781699046806 1103 6 0.15472969221914212 1103 7 0.1639102844151452 1103 8 0.15478893650941217 1103 9 0.3505032728069608 1103 10-11 0.332598690250812 1103 12-13 0.35728323541841434 1103 14-15 0.4788173071533137 1103 16-17 0.36348531315508836 1103 18-19 0.3352488192988474 1103 20-21 0.2633099120911666 1103 22-23 0.3046178182538952 1103 24-25 0.23456056681195747 1103 26-27 0.36936803515179406 1103 28-29 0.49712782255206633 1103 30-31 0.40092737741243667 1103 32-33 0.4984328626640462 1103 34-35 0.5896264480129005 1103 36-37 0.4813854988181987 1103 38-39 0.5656323357250628 1103 40-41 0.6320055317206794 1103 42-43 0.6402870325978611 1103 44-45 0.7309238849424773 1103 46-47 0.6098612451769867 1103 48-49 0.6102633204811667 1103 50-51 0.6599511101823481 1103 52-53 0.6654221941269753 1103 54-55 0.6536817173594329 1103 56-57 0.6471913736410002 1103 58-59 0.747034927508281 1103 60-61 0.7495315059510119 1103 62-63 0.6754274047806348 1103 64-65 0.6669085797590029 1103 66-67 0.6343628844538713 1103 68-69 0.5777121428088847 1103 70-71 0.41195363905219295 1103 72-73 0.6023586169982735 1103 74-75 0.8633940570955332 1103 76-77 0.7735233036619356 1103 78-79 0.7199514168318188 1103 80-81 0.9034759907394267 1103 82-83 0.69702213081694 1103 84-85 0.6249534113497432 1103 86-87 0.5824579737781121 1103 88-89 0.635379807541856 1103 90-91 0.6631201549271211 1103 92-93 0.6446313878670473 1103 94-95 0.5579223133408355 1103 96-97 0.5431471870196347 1103 98-99 0.5963243576521542 1103 100-101 0.8664142809338955 1104 1 0.24452988586434543 1104 2 0.2265236403780051 1104 3 0.22723618736242912 1104 4 0.1309052350876243 1104 5 0.08285761449694462 1104 6 0.09611619611512623 1104 7 0.16087954537510996 1104 8 0.11397913208806187 1104 9 0.11533234697328254 1104 10-11 0.2608863421368639 1104 12-13 0.20747765451734068 1104 14-15 0.2764867526160941 1104 16-17 0.2692690294640627 1104 18-19 0.16303280923339258 1104 20-21 0.2658886740096946 1104 22-23 0.20122288145822154 1104 24-25 0.3884268713784351 1104 26-27 0.13807417861149673 1104 28-29 0.19229037699877694 1104 30-31 0.28984600137984273 1104 32-33 0.24147335182455265 1104 34-35 0.2528796297939593 1104 36-37 0.35076994380549564 1104 38-39 0.4372340669901078 1104 40-41 0.46080677950984494 1104 42-43 0.5458388388624726 1104 44-45 0.46536779399838935 1104 46-47 0.5328222132317251 1104 48-49 0.4199671649911423 1104 50-51 0.4161844880476053 1104 52-53 0.49503070805377547 1104 54-55 0.31497462292714573 1104 56-57 0.45319932857974976 1104 58-59 0.5065579988457216 1104 60-61 0.682690859614894 1104 62-63 0.5022862291876109 1104 64-65 0.5283485148807543 1104 66-67 0.45278739508302124 1104 68-69 0.4781626351404711 1104 70-71 0.19565845857421493 1104 72-73 0.3316805311187352 1104 74-75 0.3410566301021234 1104 76-77 0.23914564451808573 1104 78-79 0.3521535759284724 1104 80-81 0.49679892582971164 1104 82-83 0.4893519889169795 1104 84-85 0.43862747804438484 1104 86-87 0.29962281692242954 1104 88-89 0.0893891468704382 1104 90-91 0.21118439542349066 1104 92-93 0.09822551630066911 1104 94-95 -0.04582296317123635 1104 96-97 0.2579780818212072 1104 98-99 0.14717947291250155 1104 100-101 0.36581435510268534 1105 1 0.20171814148499578 1105 2 0.22843828761077845 1105 3 0.15272062831337507 1105 4 0.1342086910694249 1105 5 0.1619478594854158 1105 6 0.1445441422713003 1105 7 0.2551370915565343 1105 8 0.014048046324134589 1105 9 0.1928461652030009 1105 10-11 0.1856796447908451 1105 12-13 0.19448592386277141 1105 14-15 0.22553612445866378 1105 16-17 0.2444276066010147 1105 18-19 0.1696408098895077 1105 20-21 0.2069860542889046 1105 22-23 0.29041655586440385 1105 24-25 0.13022584140274063 1105 26-27 0.25307766787099695 1105 28-29 0.22195144161667457 1105 30-31 0.2770536829038974 1105 32-33 0.14097959348018207 1105 34-35 0.2817845974616091 1105 36-37 0.37627474330643196 1105 38-39 0.2736770690379373 1105 40-41 0.284365105666879 1105 42-43 0.41316151534769574 1105 44-45 0.5443671331620337 1105 46-47 0.6080483255942326 1105 48-49 0.5912673069929326 1105 50-51 0.6702923970721031 1105 52-53 0.6268750003473968 1105 54-55 0.8754436897253228 1105 56-57 0.8534729170082542 1105 58-59 0.8432200095374043 1105 60-61 0.5836975010021206 1105 62-63 0.8423689117549955 1105 64-65 0.4743869261299949 1105 66-67 0.623408737302988 1105 68-69 0.6394321086741783 1105 70-71 0.5498491445194418 1105 72-73 0.6224332857829609 1105 74-75 0.6556167311908538 1105 76-77 0.4502870333776414 1105 78-79 0.8684037632743085 1105 80-81 0.7233246285898041 1105 82-83 0.7552705786682488 1105 84-85 0.7920729539645031 1105 86-87 0.619331526353669 1105 88-89 0.6018802604178042 1105 90-91 0.5559949066771246 1105 92-93 0.6611570241143951 1105 94-95 0.5828926082889048 1105 96-97 0.5258490601457027 1105 98-99 0.3542636676737878 1105 100-101 0.6333587256732969 1106 1 0.1106512785250473 1106 2 0.10858398383572165 1106 3 0.07134966761908856 1106 4 0.025756651837660627 1106 5 0.039457265205257386 1106 6 0.11968256712973613 1106 7 0.09444325305930334 1106 8 0.063196433738959 1106 9 0.12183326048815246 1106 10-11 -0.024922769437282 1106 12-13 0.04204883966954043 1106 14-15 0.1252248786013297 1106 16-17 0.10013635756174466 1106 18-19 -0.13088118235287283 1106 20-21 -0.02680462446907228 1106 22-23 0.03756066785016543 1106 24-25 0.10335595740847481 1106 26-27 0.05990412533741818 1106 28-29 0.04413740037266933 1106 30-31 -0.0035107051024922953 1106 32-33 9.839406145459861E-4 1106 34-35 -0.12341930330720885 1106 36-37 -0.10967437729517115 1106 38-39 -0.040935016443746974 1106 40-41 0.009385889002565762 1106 42-43 -0.1130053204986865 1106 44-45 -0.14543906083219582 1106 46-47 -0.2591215106711786 1106 48-49 -0.11928749042836273 1106 50-51 -0.1940831729290906 1106 52-53 -0.2771827358711789 1106 54-55 -0.4091720482455443 1106 56-57 -0.2760347140051991 1106 58-59 -0.20749188973455546 1106 60-61 -0.29060813391478035 1106 62-63 -0.23539437557490572 1106 64-65 -0.36664744536176386 1106 66-67 -0.2318910693167524 1106 68-69 -0.24591894483366872 1106 70-71 -0.3620211092053083 1106 72-73 -0.5184233909896676 1106 74-75 -0.31517175831440625 1106 76-77 -0.3121779262545097 1106 78-79 -0.1592851297973894 1106 80-81 -0.0744871270474654 1106 82-83 -0.01669813540907228 1106 84-85 -0.05077645816400178 1106 86-87 -0.31015190411795146 1106 88-89 -0.2648643857725155 1106 90-91 -0.1703490636906544 1106 92-93 -0.10779726599184158 1106 94-95 -0.08676495404429474 1106 96-97 -0.09341479522468887 1106 98-99 -0.3014029564178493 1106 100-101 -0.04489282999579558 1107 1 0.09405991913999401 1107 2 0.0534181004100347 1107 3 0.09114865713640796 1107 4 0.031284968054329454 1107 5 -0.01580553345014124 1107 6 0.06999182853009245 1107 7 0.0418043305454745 1107 8 0.05063704959187021 1107 9 0.08268403290630744 1107 10-11 0.1282562763587478 1107 12-13 0.05502511014523037 1107 14-15 0.13939373434545388 1107 16-17 0.010347979696426535 1107 18-19 -0.04350154390323979 1107 20-21 -0.09015211421378666 1107 22-23 -0.028387129157302127 1107 24-25 -0.0322667248279771 1107 26-27 0.06389103687907749 1107 28-29 0.05313599830721216 1107 30-31 -0.03608346128866202 1107 32-33 0.035464644679180424 1107 34-35 0.03716451856184477 1107 36-37 0.11693681011625756 1107 38-39 0.2101680070851515 1107 40-41 0.2039895962175038 1107 42-43 0.06835550508331778 1107 44-45 0.091684977840373 1107 46-47 0.06885452223022526 1107 48-49 0.19974952666416357 1107 50-51 0.2172101664680497 1107 52-53 0.21448726814872998 1107 54-55 0.3663219365994763 1107 56-57 0.33298322926508206 1107 58-59 0.2720482493859322 1107 60-61 0.25659844485693384 1107 62-63 0.36916056480148995 1107 64-65 0.2494681243527168 1107 66-67 0.35573764504427174 1107 68-69 0.1731805957806003 1107 70-71 0.2753426532475416 1107 72-73 0.32390778607407356 1107 74-75 0.28518132234546556 1107 76-77 0.3444725325655682 1107 78-79 0.5092634647055156 1107 80-81 0.6287254431217981 1107 82-83 0.45187813838512625 1107 84-85 0.46132644393804156 1107 86-87 0.3854844970914897 1107 88-89 0.25585586408022465 1107 90-91 0.37148003968885845 1107 92-93 0.4011046052640097 1107 94-95 0.3987005897632727 1107 96-97 0.33654264503668685 1107 98-99 0.41435787338769003 1107 100-101 0.24877540013601873 1108 1 0.010300590338268023 1108 2 0.04559865643437888 1108 3 0.072776139478691 1108 4 -0.010325216785062707 1108 5 0.006121936806387396 1108 6 0.04081762641643394 1108 7 0.0028675281300678535 1108 8 -0.007496496857150703 1108 9 0.017083452790302545 1108 10-11 0.1426056547047807 1108 12-13 -0.004552012001774131 1108 14-15 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2316 66-67 -0.9920422058881471 2316 68-69 -1.1892918905748289 2316 70-71 -1.1715811067676256 2316 72-73 -0.6778933972870291 2316 74-75 -0.955891485293499 2316 76-77 -0.8170626439907132 2316 78-79 -0.8981964995741372 2316 80-81 -0.7993862677629266 2316 82-83 -0.8091346032011373 2316 84-85 -0.866362010807876 2316 86-87 -0.8538336234995931 2316 88-89 -1.0934649505032574 2316 90-91 -0.816839607497915 2316 92-93 -1.0546077561699931 2316 94-95 -0.8512911287045419 2316 96-97 -0.5314902416260523 2316 98-99 -0.789815819277667 2316 100-101 -1.1904377545290785 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 9.0 5 38.0 6 71.0 7 177.0 8 431.0 9 846.0 10 1518.0 11 2523.0 12 4008.0 13 5932.0 14 7552.0 15 10471.0 16 14659.0 17 20916.0 18 18279.0 19 9818.0 20 7917.0 21 7682.0 22 7846.0 23 8696.0 24 9938.0 25 11918.0 26 14304.0 27 18004.0 28 22458.0 29 27995.0 30 35335.0 31 44594.0 32 56939.0 33 75025.0 34 103603.0 35 149252.0 36 229235.0 37 351717.0 38 255221.0 39 16826.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.84045654200246 20.133383699238195 15.399010129477334 21.627149629282016 2 15.282546826906717 21.514662892673194 40.179947824024666 23.022842456395423 3 18.70359689512842 26.480855547428554 30.98124381748407 23.83430373995895 4 12.489399051907913 17.937899710520007 38.28701567522562 31.285685562346465 5 12.786095236622252 38.54297969526137 34.22709498142749 14.443830086688886 6 29.64531794947144 37.89629297389538 17.936453927034922 14.521935149598262 7 26.641366297278697 34.50226710900425 21.379392120926227 17.476974472790825 8 26.22789923158022 34.540868538610454 20.228437976073558 19.002794253735765 9 26.906356411703904 16.350122055271576 20.969147211288373 35.77437432173615 10-11 24.06643791119269 27.612845969022626 27.650742724854084 20.6699733949306 12-13 25.70230113497225 25.352761645516438 28.27238947360281 20.672547745908506 14-15 22.749420656340302 25.953178205851952 27.619322883236798 23.67807825457095 16-17 20.85105519166673 29.31575582759145 28.056118358814143 21.777070621927674 18-19 20.72885451909135 27.122345738682135 31.769840502658287 20.37895923956823 20-21 21.91123759294417 26.13540430149935 31.896299504168873 20.057058601387606 22-23 21.432113563753948 26.306165225530897 31.653560306174892 20.608160904540263 24-25 21.02182790321167 26.478288106667218 31.94357795090462 20.556306039216494 26-27 21.020671210143878 26.593423236180257 31.756456732240967 20.629448821434902 28-29 20.731547780539533 26.570371148463895 32.01341581220183 20.684665258794745 30-31 21.039205008651717 26.54353408970075 31.826237421175925 20.591023480471605 32-33 20.834474567292418 26.588871567195106 31.65034353021848 20.926310335293998 34-35 21.179457337364894 26.671783450490928 31.55985863756931 20.58890057457487 36-37 21.269160880015647 26.439170640709474 31.45630905276156 20.83535942651332 38-39 21.03974458998759 26.711608980599657 31.51089124417637 20.737755185236388 40-41 21.268257788862144 26.583598359255927 31.354612739933042 20.79353111194888 42-43 21.129127259698045 26.691760818066758 31.10411688435553 21.074995037879667 44-45 21.286626247941044 27.01858410729522 30.700348508399518 20.994441136364223 46-47 21.47555293199519 27.15062762989282 30.067752515626704 21.306066922485282 48-49 21.555154709267217 27.389337102061777 29.70003550905223 21.35547267961877 50-51 21.724737732830988 27.59244603528346 29.301420025753032 21.38139620613252 52-53 21.749456744488178 27.749207994040297 29.18200412951521 21.31933113195631 54-55 21.58805191038933 27.674424224848355 29.23057589598589 21.506947968776434 56-57 21.66843997569138 27.935481625790338 28.885882892003867 21.510195506514414 58-59 21.642200788398668 27.599173322042876 29.203810142464683 21.55481574709377 60-61 21.759452740726896 27.59038687402629 29.29815544882897 21.352004936417842 62-63 21.722572125872514 27.545906191737174 29.433806986802193 21.29771469558812 64-65 21.67841518847525 27.506395176301936 29.27075173891763 21.544437896305187 66-67 21.59692034151549 27.862054654464806 28.944684660126224 21.596340343893477 68-69 21.804847304557633 27.710860454880265 29.04806125217416 21.43623098838794 70-71 21.680458684202218 27.581403621604867 29.046143594654527 21.691994099538388 72-73 21.682049239700056 27.753171245994864 28.855954157741294 21.70882535656379 74-75 21.669063950775765 27.904580359283287 28.642260767801126 21.784094922139822 76-77 21.584965677593644 27.965994653798663 28.71640257875594 21.732637089851757 78-79 21.600397748839523 27.897518451950177 28.628668720714195 21.87341507849611 80-81 21.639851469196998 28.149738485769447 28.390372822111036 21.820037222922515 82-83 21.809627020855547 28.140118451806085 28.311554223168773 21.738700304169594 84-85 21.699279641286818 28.01378525866694 28.14158408024967 22.14535101979657 86-87 21.478560203595336 27.917056425977737 28.39833331775907 22.206050052667855 88-89 21.781287497889483 27.7134777806276 28.384138207473608 22.12109651400931 90-91 21.512058030115753 27.883622706293426 28.5458767092037 22.058442554387124 92-93 21.488218178771778 27.723899713550143 28.49927984765636 22.28860226002172 94-95 21.29490718901683 27.670522943040016 28.556293644103842 22.478276223839313 96-97 21.39768876721792 27.611317977529538 28.537351310733982 22.453641944518555 98-99 21.16704167688372 27.575092533489116 28.45394720943099 22.803918580196175 100-101 21.171263191540916 27.927304105295175 28.234827472431164 22.666605230732745 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 773.0 1 552.5 2 392.5 3 468.5 4 413.0 5 384.5 6 656.5 7 1369.0 8 2321.5 9 2814.0 10 2610.0 11 2334.5 12 2368.0 13 2590.5 14 2843.5 15 3034.0 16 3171.5 17 3326.0 18 3567.5 19 3904.5 20 4276.5 21 4728.0 22 5472.5 23 6447.5 24 7576.5 25 9090.5 26 11343.5 27 14141.0 28 16292.5 29 18654.0 30 21853.0 31 25206.0 32 28881.0 33 32420.5 34 36410.5 35 39886.0 36 44307.5 37 48835.5 38 50325.5 39 50381.0 40 50480.5 41 51075.5 42 51362.0 43 52664.5 44 54844.0 45 56145.5 46 56163.5 47 55847.0 48 58220.5 49 57192.0 50 52647.0 51 49792.5 52 46813.0 53 44749.5 54 42542.5 55 39491.0 56 35835.0 57 31840.0 58 29524.5 59 26882.0 60 22771.0 61 18433.0 62 14469.0 63 10827.0 64 7413.5 65 5224.0 66 3679.0 67 2547.5 68 1738.0 69 1160.0 70 833.5 71 632.5 72 475.5 73 406.0 74 335.5 75 273.0 76 191.5 77 82.5 78 38.0 79 24.5 80 19.0 81 12.5 82 7.0 83 4.0 84 2.5 85 2.0 86 1.5 87 1.5 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028483859575861154 2 0.0054132221818378665 3 6.444312121235555E-5 4 5.155449696988444E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011084216848525154 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.8665872727413334E-4 20-21 0.0011599761818223999 22-23 0.0018366289545521332 24-25 0.01056867187882631 26-27 0.0052198928182008 28-29 3.2221560606177774E-4 30-31 0.0026421679697065776 32-33 0.0047043478485019555 34-35 0.0 36-37 0.008023168590938266 38-39 0.013629720136413198 40-41 0.0013533055454594666 42-43 0.0 44-45 0.0 46-47 3.544371666679555E-4 48-49 0.0025132817272818663 50-51 0.006057653393961422 52-53 0.0 54-55 0.0028032757727374665 56-57 0.0027710542121312885 58-59 1.9332936363706667E-4 60-61 0.0015144133484903555 62-63 0.0038021441515289777 64-65 3.2221560606177774E-5 66-67 0.0016432995909150664 68-69 4.5110184848648884E-4 70-71 8.377605757606222E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0014821917878841777 80-81 0.0015466349090965334 82-83 0.009956462227308933 84-85 0.0063476474394170215 86-87 0.0032543776212239552 88-89 1.288862424247111E-4 90-91 6.444312121235555E-4 92-93 6.444312121235555E-5 94-95 0.0 96-97 0.0019332936363706664 98-99 0.0013210839848532888 100-101 2.577724848494222E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1551756.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.64379963675893 #Duplication Level Percentage of deduplicated Percentage of total 1 76.44891268384387 44.06805805195575 2 13.81969461082479 15.932394143751626 3 4.377737340915764 7.570482424263185 4 1.896774722274142 4.3734920818735885 5 0.9903644690382457 2.8544185510302893 6 0.567957520490619 1.9643537708010985 7 0.3652688334250963 1.473883841325625 8 0.24904528001948376 1.1484732977538916 9 0.18867002709978845 0.9788091515641875 >10 0.9801887570377452 10.405734741072163 >50 0.07707680343762915 3.0813628436918825 >100 0.03629277247937776 3.696613190608919 >500 0.001344119408957259 0.5338382327959185 >1k 3.3602985223931477E-4 0.3021080178098817 >5k 2.240199014928765E-4 0.9109082956319323 >10k+ 1.1200995074643825E-4 0.7050693640700809 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10920 0.7037188836389225 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8465 0.5455110210625898 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5643 0.3636525330013224 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2106 0.13571721327322078 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1552 0.1000157241215758 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.2221560606177774E-5 0.0 12-13 0.0 0.0 0.0 6.444312121235555E-5 0.0 14-15 0.0 0.0 0.0 6.444312121235555E-5 0.0 16-17 0.0 0.0 0.0 6.444312121235555E-5 0.0 18-19 0.0 0.0 0.0 1.6110780303088887E-4 0.0 20-21 0.0 0.0 0.0 2.2555092424324442E-4 0.0 22-23 0.0 3.2221560606177774E-5 0.0 3.866587272741333E-4 0.0 24-25 6.444312121235555E-5 6.444312121235555E-5 0.0 6.122096515173777E-4 0.0 26-27 6.444312121235555E-5 6.444312121235555E-5 0.0 9.344252575791555E-4 0.0 28-29 6.444312121235555E-5 6.444312121235555E-5 0.0 0.0014177486666718223 0.0 30-31 1.288862424247111E-4 1.288862424247111E-4 0.0 0.0032543776212239552 0.0 32-33 1.288862424247111E-4 2.577724848494222E-4 0.0 0.0059609887121428885 0.0 34-35 1.288862424247111E-4 2.577724848494222E-4 0.0 0.008603156681849466 0.0 36-37 1.288862424247111E-4 2.577724848494222E-4 0.0 0.011374210893980755 0.0 38-39 1.288862424247111E-4 2.577724848494222E-4 0.0 0.01437081603035529 0.0 40-41 1.288862424247111E-4 2.577724848494222E-4 0.0 0.017335199606123643 0.0 42-43 1.6110780303088887E-4 2.577724848494222E-4 0.0 0.022780643348567685 0.0 44-45 1.9332936363706664E-4 2.577724848494222E-4 0.0 0.030610482575868885 0.0 46-47 1.9332936363706664E-4 2.577724848494222E-4 0.0 0.04791346062138635 0.0 48-49 1.9332936363706664E-4 2.577724848494222E-4 0.0 0.08332495572757573 0.0 50-51 1.9332936363706664E-4 2.577724848494222E-4 0.0 0.10816777895493879 0.0 52-53 1.9332936363706664E-4 2.577724848494222E-4 0.0 0.13523388986412813 0.0 54-55 1.9332936363706664E-4 2.577724848494222E-4 0.0 0.1739319841521476 0.0 56-57 1.9332936363706664E-4 2.577724848494222E-4 0.0 0.20309249650073852 0.0 58-59 1.9332936363706664E-4 2.577724848494222E-4 0.0 0.23089970330386994 0.0 60-61 1.9332936363706664E-4 2.577724848494222E-4 0.0 0.2838719489404262 0.0 62-63 1.9332936363706664E-4 2.577724848494222E-4 0.0 0.3189612284405538 0.0 64-65 1.9332936363706664E-4 3.2221560606177774E-4 0.0 0.35530714880432235 0.0 66-67 1.9332936363706664E-4 3.2221560606177774E-4 0.0 0.4381165595621992 0.0 68-69 1.9332936363706664E-4 3.2221560606177774E-4 0.0 0.5374234093504391 0.0 70-71 1.9332936363706664E-4 3.866587272741333E-4 0.0 0.6182995264719453 0.0 72-73 2.2555092424324442E-4 3.866587272741333E-4 0.0 0.7155764179419961 0.0 74-75 2.577724848494222E-4 3.866587272741333E-4 0.0 0.7979347268513864 0.0 76-77 2.577724848494222E-4 3.866587272741333E-4 0.0 0.8579312727000894 0.0 78-79 2.577724848494222E-4 3.866587272741333E-4 0.0 0.9376151920791671 0.0 80-81 3.2221560606177774E-4 3.866587272741333E-4 0.0 1.0809044720948395 0.0 82-83 4.5110184848648884E-4 3.866587272741333E-4 0.0 1.2107895828983422 0.0 84-85 4.833234090926666E-4 3.866587272741333E-4 0.0 1.3622953608685902 0.0 86-87 5.155449696988444E-4 3.866587272741333E-4 0.0 1.6526760650514642 0.0 88-89 5.155449696988444E-4 3.866587272741333E-4 0.0 2.00273109947698 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5575 0.0 47.88185 1 GTATCAA 9900 0.0 40.109802 1 ATCAACG 12615 0.0 31.321716 3 TCAACGC 12750 0.0 30.990078 4 CAACGCA 12985 0.0 30.4766 5 TATCAAC 12995 0.0 30.405807 2 AACGCAG 13425 0.0 29.477737 6 ACGCAGA 15030 0.0 26.298306 7 CGCAGAG 15125 0.0 26.007488 8 GCAGAGT 15840 0.0 24.593601 9 CGTCGAG 50 0.0016541255 23.750374 38-39 GAGTACT 9230 0.0 23.668222 12-13 GTACATG 8600 0.0 22.534151 1 GTACTTT 9770 0.0 22.239243 14-15 TACATGG 8640 0.0 21.931517 2 AGTACTT 9680 0.0 21.120651 12-13 ACATGGG 8540 0.0 20.9093 3 CAGAGTA 15515 0.0 20.576948 10-11 AGAGTAC 15060 0.0 20.346899 10-11 GTAGGAC 460 0.0 19.615767 3 >>END_MODULE