FastQCFastQC Report
Thu 26 May 2016
SRR936824_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936824_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571823
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97100.6177540346463947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71660.4559037499769376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47450.301878773882301No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16720.1063733003016243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51250.046.276881
GTATCAA90700.040.4545481
GTGCGTA801.073382E-535.6466451
ATCAACG117700.030.813923
TCAACGC118700.030.6344134
TATCAAC119750.030.3261092
CAACGCA120100.030.2358065
AACGCAG123600.029.4555856
ACGCAGA139350.025.9517047
CGCAGAG140050.025.653158
GTACATG92500.023.9956471
GCAGAGT146100.023.9387789
TACATGG93950.023.3745922
GAGTACT80300.022.31251712-13
ACATGGG92550.022.2387473
CATGGGG31700.021.5924574
AGTACTT84400.021.28492412-13
GTACTTT86550.020.6166114-15
AGAGTAC139200.020.26076710-11
CAGAGTA143350.019.9062610-11
Produced by FastQC (version 0.11.5)