##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936824_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1571823 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.791068714479938 33.0 31.0 34.0 30.0 34.0 2 31.86812382819185 33.0 31.0 34.0 30.0 34.0 3 31.886391788388387 33.0 31.0 34.0 30.0 34.0 4 35.49769916841782 37.0 35.0 37.0 33.0 37.0 5 35.50822198173713 37.0 35.0 37.0 33.0 37.0 6 35.57791557955317 37.0 35.0 37.0 33.0 37.0 7 35.52589954466883 37.0 35.0 37.0 33.0 37.0 8 35.49591461634039 37.0 35.0 37.0 33.0 37.0 9 37.118843533909356 39.0 37.0 39.0 33.0 39.0 10-11 36.99430342983911 39.0 37.0 39.0 33.0 39.0 12-13 36.91209410983298 39.0 37.0 39.0 33.0 39.0 14-15 38.03200074054139 40.0 38.0 41.0 33.0 41.0 16-17 37.973940768139926 40.0 38.0 41.0 33.0 41.0 18-19 37.975638478378286 40.0 38.0 41.0 33.0 41.0 20-21 37.92548334004529 40.0 38.0 41.0 33.0 41.0 22-23 37.8188867321575 40.0 37.5 41.0 32.5 41.0 24-25 37.771388699618214 40.0 37.0 41.0 32.5 41.0 26-27 37.79032785498113 40.0 37.0 41.0 33.0 41.0 28-29 37.709401122136526 40.0 37.0 41.0 32.0 41.0 30-31 37.525457701026134 40.0 37.0 41.0 32.0 41.0 32-33 37.31993996779536 40.0 36.5 41.0 32.0 41.0 34-35 37.14928016704171 39.5 36.0 41.0 31.0 41.0 36-37 36.96270413398965 39.0 36.0 41.0 31.0 41.0 38-39 36.794750744835774 39.0 36.0 41.0 30.5 41.0 40-41 36.600624243314925 39.0 35.0 41.0 30.0 41.0 42-43 36.35643580733963 39.0 35.0 41.0 30.0 41.0 44-45 36.0607294841722 39.0 35.0 40.5 29.5 41.0 46-47 35.66347705816749 39.0 35.0 40.0 27.5 41.0 48-49 35.36331094531636 39.0 35.0 40.0 26.5 41.0 50-51 34.64928939199898 38.0 34.0 39.5 25.0 40.5 52-53 34.82122414546676 38.5 34.5 40.0 26.0 40.5 54-55 35.20485544491969 39.0 35.0 40.5 26.0 41.0 56-57 35.10338091502669 39.0 35.0 41.0 26.0 41.0 58-59 34.90616500712866 38.5 35.0 40.5 26.0 41.0 60-61 34.642569487785835 38.0 34.0 40.0 25.0 41.0 62-63 34.34376421518199 38.0 34.0 40.0 24.0 41.0 64-65 34.02293674287753 37.5 34.0 40.0 23.0 41.0 66-67 33.67225699076805 37.0 33.5 40.0 22.5 41.0 68-69 33.28784379666158 36.0 33.0 39.0 22.0 41.0 70-71 32.8926526714522 36.0 33.0 39.0 21.0 41.0 72-73 32.515686244570794 35.0 32.5 38.5 20.0 40.0 74-75 32.11744770244486 35.0 32.0 37.5 18.5 40.0 76-77 31.667938756463037 35.0 32.0 37.0 17.5 39.0 78-79 31.21542342871939 35.0 31.0 36.5 13.5 39.0 80-81 30.788386160528255 35.0 31.0 36.0 9.0 38.5 82-83 30.39592625887266 35.0 31.0 36.0 7.0 37.0 84-85 30.024486535697722 34.0 30.0 35.0 4.5 37.0 86-87 29.678787942408274 34.0 30.0 35.0 2.0 36.5 88-89 29.31899361442096 34.0 29.5 35.0 2.0 36.0 90-91 28.964295598168498 34.0 29.0 35.0 2.0 36.0 92-93 28.611111429213086 34.0 29.0 35.0 2.0 35.0 94-95 28.26093077910172 34.0 29.0 35.0 2.0 35.0 96-97 27.786842093543612 34.0 29.0 35.0 2.0 35.0 98-99 27.25643218097712 34.0 27.0 35.0 2.0 35.0 100-101 25.12610739249903 31.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24897293796655262 1101 2 0.2956067414950745 1101 3 0.29402146181731936 1101 4 0.34090189790398995 1101 5 0.2398015574289829 1101 6 0.28719014631943907 1101 7 0.13129626263312844 1101 8 0.3019926312919523 1101 9 0.2162776290621835 1101 10-11 0.41306488968346855 1101 12-13 0.37271317403004645 1101 14-15 0.44500344100748634 1101 16-17 0.508984170186018 1101 18-19 0.595243695779871 1101 20-21 0.4719053834113254 1101 22-23 0.5492306137110532 1101 24-25 0.6071371400010719 1101 26-27 0.40768590844022157 1101 28-29 0.5132141485507233 1101 30-31 0.4733061763458011 1101 32-33 0.30219272989798895 1101 34-35 0.3729912356410878 1101 36-37 0.31712639816448984 1101 38-39 0.3344696763481778 1101 40-41 0.45712104271636633 1101 42-43 0.4118526058790266 1101 44-45 0.2832440432638066 1101 46-47 0.38852605996891754 1101 48-49 0.32548500521065904 1101 50-51 0.2987162625475861 1101 52-53 0.23840900607125093 1101 54-55 0.4543435230817394 1101 56-57 0.35782467586652444 1101 58-59 0.48729584220553335 1101 60-61 0.3250734655145351 1101 62-63 0.39130376040332493 1101 64-65 0.29163430092688003 1101 66-67 0.2140944360758823 1101 68-69 0.23126766409847477 1101 70-71 0.007457086223801923 1101 72-73 0.09337289048757214 1101 74-75 0.20252553660267836 1101 76-77 0.14957321076010643 1101 78-79 0.32484012828308195 1101 80-81 0.4000918235503761 1101 82-83 0.4097487102419777 1101 84-85 0.2588009259187878 1101 86-87 0.40693918435656684 1101 88-89 0.5210506727521924 1101 90-91 0.5965259371961764 1101 92-93 0.5676927943260921 1101 94-95 0.34359027751458626 1101 96-97 0.30264734279141337 1101 98-99 0.3228168376705689 1101 100-101 0.5545677775887405 1102 1 0.1883551128869705 1102 2 0.24190848148390032 1102 3 0.3383165935522534 1102 4 0.3173257272792256 1102 5 0.28999045842036963 1102 6 0.30600077726770536 1102 7 0.17268387930190698 1102 8 -0.008425429713369681 1102 9 0.21648067393327608 1102 10-11 0.23520414419893854 1102 12-13 0.1975042237246427 1102 14-15 0.12297081238931895 1102 16-17 0.3005008315304991 1102 18-19 0.0436358501552192 1102 20-21 0.11152151559973333 1102 22-23 0.039768248825204466 1102 24-25 0.09142576846760164 1102 26-27 0.16840609438120424 1102 28-29 -0.07437127975365598 1102 30-31 0.008003883724974514 1102 32-33 0.12690613406736873 1102 34-35 0.17065993785992362 1102 36-37 0.10249065410164349 1102 38-39 0.3908909977364985 1102 40-41 0.16410359908410044 1102 42-43 0.26228599914157513 1102 44-45 0.30788793099348766 1102 46-47 0.3723825345850287 1102 48-49 0.38576709541361254 1102 50-51 0.291569957964235 1102 52-53 0.49986828263367045 1102 54-55 0.2179717708883473 1102 56-57 0.39156056297212416 1102 58-59 0.18855705341739792 1102 60-61 0.20724619873909234 1102 62-63 0.09128965297691849 1102 64-65 0.24250035838602457 1102 66-67 -0.040276496647379645 1102 68-69 0.11196677274242717 1102 70-71 0.07201842910498613 1102 72-73 0.07359333087256914 1102 74-75 0.2193146498980738 1102 76-77 0.2806634632939726 1102 78-79 0.13334949459352785 1102 80-81 0.25338989925366917 1102 82-83 0.21651214583410194 1102 84-85 0.1562867783937456 1102 86-87 0.21751399454424458 1102 88-89 0.37776405796585877 1102 90-91 0.09756704339338285 1102 92-93 0.1322195065739642 1102 94-95 0.13736995961266274 1102 96-97 -0.06921840262750223 1102 98-99 0.15175153377400363 1102 100-101 0.2769051922333432 1103 1 0.22127838082565532 1103 2 0.2749881447626841 1103 3 0.325755794010103 1103 4 0.3303184921166391 1103 5 0.13056419028636412 1103 6 0.19136162707491167 1103 7 0.14877383189158166 1103 8 0.19072866955190904 1103 9 0.11373815529901066 1103 10-11 0.3181626523103276 1103 12-13 0.25045685164442233 1103 14-15 0.4768574414755662 1103 16-17 0.4252165539745292 1103 18-19 0.46395259511061226 1103 20-21 0.32741264805224546 1103 22-23 0.4946767063300612 1103 24-25 0.39384450094141243 1103 26-27 0.4802020592448386 1103 28-29 0.5684253489364863 1103 30-31 0.46028828011407796 1103 32-33 0.5064105247470252 1103 34-35 0.47681423108274856 1103 36-37 0.4500227834412698 1103 38-39 0.6191315569898421 1103 40-41 0.61791302077647 1103 42-43 0.5224102070913759 1103 44-45 0.570190917256646 1103 46-47 0.526322843469238 1103 48-49 0.6610399677198942 1103 50-51 0.5427825340618355 1103 52-53 0.4532421535481035 1103 54-55 0.6124228437330643 1103 56-57 0.6186771587714333 1103 58-59 0.7681168654151733 1103 60-61 0.922921519281239 1103 62-63 0.7055950118051939 1103 64-65 0.7398901269892235 1103 66-67 0.6400741362415374 1103 68-69 0.7558188992409853 1103 70-71 0.6284706588654458 1103 72-73 0.525162679571352 1103 74-75 0.6183080170965738 1103 76-77 0.6444047061793476 1103 78-79 0.7230917359504687 1103 80-81 0.5213729730435865 1103 82-83 0.7039863474324761 1103 84-85 0.7261570795333157 1103 86-87 0.6556545154158506 1103 88-89 0.5753408863229978 1103 90-91 0.7052127949720202 1103 92-93 0.6041079564024763 1103 94-95 0.771106073550424 1103 96-97 0.7460378945045214 1103 98-99 0.8721854727921148 1103 100-101 1.0475708900229073 1104 1 0.18825755604951055 1104 2 0.17067360986304791 1104 3 0.15842885460869027 1104 4 0.26920475680077516 1104 5 0.15495765670110728 1104 6 0.12650326567399617 1104 7 0.013553124862362154 1104 8 0.13673348896098503 1104 9 0.19224557609366144 1104 10-11 0.2586071811857025 1104 12-13 0.14712177264444648 1104 14-15 0.048532117671520325 1104 16-17 0.2820427951092839 1104 18-19 0.37335745970164425 1104 20-21 0.28057196238177085 1104 22-23 0.2143532787991873 1104 24-25 0.09488762233027614 1104 26-27 0.12209275313021095 1104 28-29 0.19460281819906555 1104 30-31 0.40321935708771406 1104 32-33 0.032665522145642 1104 34-35 0.14848343067748715 1104 36-37 0.27913639553361236 1104 38-39 0.22757236862214825 1104 40-41 0.13402569500084383 1104 42-43 0.2559610641794663 1104 44-45 0.2875515719843236 1104 46-47 0.13634023167811193 1104 48-49 0.24756581727766047 1104 50-51 0.19990399823117855 1104 52-53 0.3493090981522826 1104 54-55 0.5593591987811237 1104 56-57 0.5539326079896512 1104 58-59 0.33688586237565943 1104 60-61 0.47615596573377417 1104 62-63 0.2662938945785456 1104 64-65 0.26410919788381904 1104 66-67 0.06673628471220638 1104 68-69 0.09913522088177018 1104 70-71 0.30663767466543845 1104 72-73 0.2817251881285401 1104 74-75 0.36852571199511885 1104 76-77 0.3878854970005712 1104 78-79 0.4870156522680027 1104 80-81 0.45545061071165094 1104 82-83 0.4142732871077115 1104 84-85 0.400956499013585 1104 86-87 0.49393493108988196 1104 88-89 0.3260016724891024 1104 90-91 0.41065379828536663 1104 92-93 0.36213340206090194 1104 94-95 0.30487787726903903 1104 96-97 0.26241122071827405 1104 98-99 0.37636908269380953 1104 100-101 0.2684933892698744 1105 1 0.1836615373141477 1105 2 0.15821264186929085 1105 3 0.15873135265709593 1105 4 0.08902325797173205 1105 5 0.050020107911095124 1105 6 0.02048257871068415 1105 7 0.020481305970875496 1105 8 0.09918469895318083 1105 9 0.17742317172207578 1105 10-11 0.17173657228640593 1105 12-13 0.1391641223712199 1105 14-15 0.18962306224607772 1105 16-17 0.2449135991686049 1105 18-19 0.1469181544713578 1105 20-21 0.09891700531698433 1105 22-23 0.2644069478077782 1105 24-25 0.16086634988687365 1105 26-27 0.27896726523012916 1105 28-29 0.26739750984621224 1105 30-31 0.2757017926849059 1105 32-33 0.13155798896843862 1105 34-35 -0.02808760757388029 1105 36-37 -0.004997093927755714 1105 38-39 0.38371990960575175 1105 40-41 0.2956523538611151 1105 42-43 0.48462519376573 1105 44-45 0.37696901929110993 1105 46-47 0.21653148850871418 1105 48-49 0.10448424038460757 1105 50-51 0.0336618046324233 1105 52-53 0.06874215826157126 1105 54-55 -0.051750285996398304 1105 56-57 0.16368573055015645 1105 58-59 0.3093682979728527 1105 60-61 0.22650237478919877 1105 62-63 0.2721779695199942 1105 64-65 0.41444584893768877 1105 66-67 0.45346540258886137 1105 68-69 0.4087126994141741 1105 70-71 0.4022514529636467 1105 72-73 0.43807276814415275 1105 74-75 0.48450931855249735 1105 76-77 0.5808402198733269 1105 78-79 0.5411628029315452 1105 80-81 0.1750690728506008 1105 82-83 0.09828480154955344 1105 84-85 0.22287297052022126 1105 86-87 0.4886948842514762 1105 88-89 0.48594019707926606 1105 90-91 0.41084895831659196 1105 92-93 0.5066152521779941 1105 94-95 0.5407775650129878 1105 96-97 0.38316649003911607 1105 98-99 0.28720534342761184 1105 100-101 0.5730357389966549 1106 1 0.23505417546063612 1106 2 0.28014743756206784 1106 3 0.2162305630783372 1106 4 0.18271348090109996 1106 5 0.08328371995083472 1106 6 0.22548999777141177 1106 7 0.35620125281583626 1106 8 0.4294088797279798 1106 9 0.3513286367988613 1106 10-11 0.40083955123295567 1106 12-13 0.48631433898395926 1106 14-15 0.4225279373734523 1106 16-17 0.45077689180138947 1106 18-19 0.5157125124097561 1106 20-21 0.4643415007314644 1106 22-23 0.4338393747642968 1106 24-25 0.367727044358638 1106 26-27 0.3362944070742344 1106 28-29 0.24276712208855855 1106 30-31 0.41731423167876613 1106 32-33 0.3259656857225224 1106 34-35 0.32957666949972975 1106 36-37 0.21566856565356574 1106 38-39 0.21881191787696253 1106 40-41 0.37061466264897547 1106 42-43 0.10864417941029814 1106 44-45 0.13414599219515821 1106 46-47 0.020327872535979452 1106 48-49 0.012046915060281549 1106 50-51 -0.04206028748310331 1106 52-53 -0.07061774663143439 1106 54-55 -0.06455710438135753 1106 56-57 -0.09445661411139383 1106 58-59 -0.04243074976537997 1106 60-61 0.011645333236501187 1106 62-63 -0.0752500894272643 1106 64-65 -0.1264692281430797 1106 66-67 -0.11064466766875114 1106 68-69 0.012032803578975404 1106 70-71 -0.017364960739868707 1106 72-73 -0.05139231459774152 1106 74-75 0.2761774749903978 1106 76-77 0.09219302153710274 1106 78-79 0.24178210083215745 1106 80-81 0.30264935662222925 1106 82-83 0.22426283636253075 1106 84-85 0.03463516131820299 1106 86-87 0.4623236988107138 1106 88-89 0.4299888589667482 1106 90-91 0.35653711344348693 1106 92-93 0.4697828931550454 1106 94-95 0.6323269866837968 1106 96-97 0.7220395376939166 1106 98-99 0.7942512164633477 1106 100-101 0.8458039127158976 1107 1 0.38607882467212207 1107 2 0.41839205424655646 1107 3 0.216022995912045 1107 4 0.27925296994399673 1107 5 0.38476526112483356 1107 6 0.3745781674197204 1107 7 0.4269865404550899 1107 8 0.41990230351176194 1107 9 0.4627201670816916 1107 10-11 0.5833918215293679 1107 12-13 0.5873403312771117 1107 14-15 0.5301368612964197 1107 16-17 0.6334903318975051 1107 18-19 0.6558893352157682 1107 20-21 0.6938271492933907 1107 22-23 0.6494363379345529 1107 24-25 0.6095360776817103 1107 26-27 0.7085410896625248 1107 28-29 0.8001576120935923 1107 30-31 0.7753425633759861 1107 32-33 0.7473105500224335 1107 34-35 0.5741243684345392 1107 36-37 0.6274500780123589 1107 38-39 0.7526294931994215 1107 40-41 0.8629621496210831 1107 42-43 0.8171911848265765 1107 44-45 0.8909853303245114 1107 46-47 0.9267364477461157 1107 48-49 1.0085188837249675 1107 50-51 0.8225430394522775 1107 52-53 0.8761205184977712 1107 54-55 0.8541260162566715 1107 56-57 0.9037149633502253 1107 58-59 0.9728309313821484 1107 60-61 1.048493389186838 1107 62-63 1.032166529621044 1107 64-65 1.1166438153776497 1107 66-67 1.1468729267010502 1107 68-69 0.9354795906834354 1107 70-71 0.9294880768224658 1107 72-73 0.7923303659002272 1107 74-75 0.9016762466811628 1107 76-77 0.9504643289172101 1107 78-79 0.9008249475792134 1107 80-81 0.8630190459552303 1107 82-83 0.8423734379165992 1107 84-85 0.9406695051373397 1107 86-87 0.9254231405771343 1107 88-89 0.7439256511016623 1107 90-91 0.8957557085312331 1107 92-93 0.8544518601543096 1107 94-95 0.9779025093867588 1107 96-97 0.9178529079497864 1107 98-99 1.245842279842151 1107 100-101 1.438701208482012 1108 1 0.0025369752202344387 1108 2 -0.05448656584060885 1108 3 -0.017469947920545792 1108 4 0.03635894515061011 1108 5 -0.045586521016986126 1108 6 -0.02390451788595982 1108 7 0.040503211595058985 1108 8 -0.0027858440366088644 1108 9 0.027419181375961443 1108 10-11 -0.01695623160596682 1108 12-13 0.020295839568511553 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-2.03189726651717 2316 62-63 -1.9933816130697224 2316 64-65 -2.5270913790908622 2316 66-67 -2.2311831773744473 2316 68-69 -2.1548553343259087 2316 70-71 -1.803302652435967 2316 72-73 -2.2294667416803264 2316 74-75 -2.611275701369223 2316 76-77 -2.603796078572678 2316 78-79 -2.9438506297488765 2316 80-81 -2.918699395251913 2316 82-83 -3.2357783349813225 2316 84-85 -3.143427315877428 2316 86-87 -3.5907696489262193 2316 88-89 -3.331861843925605 2316 90-91 -3.5923578008439563 2316 92-93 -3.6580567983148065 2316 94-95 -4.037182757254026 2316 96-97 -4.296600095141823 2316 98-99 -4.8022088685242466 2316 100-101 -4.927066179266365 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2451.0 3 923.0 4 856.0 5 912.0 6 1058.0 7 1369.0 8 1717.0 9 2127.0 10 2818.0 11 3867.0 12 5250.0 13 7011.0 14 8059.0 15 10521.0 16 13353.0 17 18553.0 18 15928.0 19 8789.0 20 8196.0 21 8426.0 22 9336.0 23 10203.0 24 11529.0 25 13560.0 26 15757.0 27 18578.0 28 22743.0 29 27525.0 30 33902.0 31 42281.0 32 54254.0 33 71443.0 34 96664.0 35 139185.0 36 208738.0 37 314795.0 38 302486.0 39 56640.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.79909445355988 19.579482257577936 14.78163907215222 21.83978421670997 2 14.929141134161872 21.053378500066504 41.01681151968069 23.000668846090942 3 18.635258960213665 26.603916587491945 30.500713049797668 24.260111402496722 4 12.144907113028236 17.51323751529976 38.87071694217374 31.471138429498257 5 12.934865597851248 38.380118683213624 34.28854705078136 14.396468668153762 6 29.554757984643754 37.635481785553914 18.47819648481413 14.331563744988202 7 26.76454336655446 34.3552181950591 21.550549752751312 17.32968868563513 8 25.80573472092124 34.6209466511377 20.61692601689125 18.956392611049804 9 26.984308894173374 16.59895519964855 21.011501717458223 35.405234188719845 10-11 23.920015229615746 27.59883969683387 27.959333360498757 20.521811713051626 12-13 25.5694296551318 25.390525395968922 28.20610279991532 20.833942148983954 14-15 22.775849308854777 26.0080374977121 27.43654753662632 23.779565656806806 16-17 20.904924031080785 29.40509765340909 27.848384898581322 21.8415934169288 18-19 20.887481542101515 27.518605911811434 31.139521181662012 20.454391364425046 20-21 22.243105595637132 26.55345508782104 30.976256506249467 20.22718281029236 22-23 21.649862785629914 26.763071005254368 30.720235428292124 20.86683078082359 24-25 21.166033840177775 26.92239537684062 31.095455261261634 20.816115521719972 26-27 21.116054940660792 27.030666411752115 30.934517392351356 20.918761255235744 28-29 20.935665891600365 27.038318857970715 31.157792533976885 20.86822271645203 30-31 21.171914740107354 26.993727634197995 31.00194021195042 20.832417413744235 32-33 20.90691263121796 27.06113759665385 30.86792978634194 21.164019985786243 34-35 21.318776686884704 27.08206215057718 30.71281460381409 20.886346558724032 36-37 21.443259744506925 26.89723777856673 30.613009048766116 21.04649342816023 38-39 21.179636775451645 27.182130802828684 30.597612223279075 21.040620198440596 40-41 21.392700237641527 26.93690569735222 30.66371019822548 21.00668386678077 42-43 21.153233169291653 27.16533429192209 30.35797563869687 21.32345690008939 44-45 21.33269075078772 27.429081313731384 30.062047388872188 21.17618054660871 46-47 21.510922706498846 27.48002362904311 29.537353642604042 21.471700021854 48-49 21.49582744578097 27.651498041441158 29.29583495300668 21.556839559771195 50-51 21.657808872045983 27.88571806668811 28.95212082865313 21.50435223261278 52-53 21.686645034039575 27.9796080676974 28.829134058662593 21.50461283960043 54-55 21.56944714869833 27.95504957374596 28.88378580273072 21.591717474824986 56-57 21.56472066240221 28.280778418672835 28.57034237709041 21.584158541834547 58-59 21.660355125963594 27.863543472009077 28.83537440415747 21.640726997869855 60-61 21.740160836758324 27.929608469378664 28.879391517896906 21.450839175966102 62-63 21.676066482487965 27.86998731279372 29.004614223668955 21.44933198104936 64-65 21.58982425947693 27.828461725657732 28.964977549303256 21.616736465562077 66-67 21.583423271157447 28.15258588213733 28.53703826260487 21.72695258410035 68-69 21.66985819676905 28.07441097254096 28.722688867028562 21.533041963661432 70-71 21.626179290325044 27.9492761926588 28.67266974778147 21.751874769234686 72-73 21.579382179223185 28.107673754339462 28.485242559884057 21.827701506553296 74-75 21.566532651262055 28.2824892441423 28.338674542365016 21.812303562230625 76-77 21.619040104055966 28.216113402678523 28.388118834562974 21.776727658702534 78-79 21.5564241952753 28.222486314151666 28.31374121218196 21.907348278391076 80-81 21.500069239842038 28.6071968050986 28.047070358821554 21.84566359623781 82-83 21.69807208221421 28.49152500845606 28.085220527333515 21.725182381996216 84-85 21.680709537189745 28.305801162624967 27.935927096695373 22.07756220348991 86-87 21.59099008968416 28.26947505619579 28.127617272919352 22.011917581200695 88-89 21.86571291994549 28.02869005586649 28.193418867559433 21.912178156628585 90-91 21.582862711127603 28.226607694887875 28.303462374940093 21.887067219044432 92-93 21.624154606590537 28.165427880765954 28.216632021395625 21.99378549124788 94-95 21.407755530824936 28.109838311378017 28.31717465183593 22.16523150596112 96-97 21.45462852255959 28.084934036418847 28.254957370085183 22.20548007093638 98-99 21.287802195599063 28.047589452188582 28.196839526256717 22.467768825955638 100-101 21.36191903589788 28.3058558965811 28.17469766754827 22.15752739997275 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 482.0 1 342.5 2 265.5 3 359.5 4 340.5 5 357.5 6 596.5 7 1248.0 8 2192.5 9 2659.5 10 2420.0 11 2155.5 12 2267.5 13 2475.5 14 2635.0 15 2742.5 16 2852.5 17 3043.5 18 3250.0 19 3529.0 20 3911.0 21 4502.0 22 5239.5 23 6293.0 24 7443.5 25 8936.5 26 11255.5 27 14154.0 28 16470.5 29 18773.0 30 21987.5 31 25312.5 32 29223.5 33 33433.5 34 37864.5 35 41376.0 36 45695.5 37 50345.5 38 51787.0 39 51848.0 40 51711.5 41 52098.0 42 52608.0 43 53682.5 44 55797.0 45 57021.0 46 56692.0 47 56234.5 48 58887.0 49 57853.0 50 53167.5 51 50497.5 52 47317.5 53 45276.0 54 43250.0 55 39835.0 56 36278.0 57 32481.0 58 29742.0 59 26830.0 60 22636.0 61 18583.5 62 14885.0 63 11141.5 64 7694.5 65 5357.0 66 3577.0 67 2471.0 68 1781.5 69 1217.5 70 869.0 71 643.5 72 498.5 73 402.0 74 302.5 75 232.0 76 166.5 77 87.0 78 55.0 79 37.0 80 21.5 81 15.5 82 12.5 83 9.0 84 7.0 85 7.5 86 7.5 87 6.0 88 3.5 89 2.0 90 0.5 91 0.0 92 1.0 93 2.0 94 1.0 95 1.0 96 1.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03925378366393671 2 0.02926538166193013 3 0.02570263954656472 4 0.04580668434041237 5 0.09059544236214892 6 0.11311706216285165 7 0.059485069247618844 8 0.09059544236214892 9 0.07558102916168041 10-11 0.07628085350577005 12-13 0.07319526435228395 14-15 0.0674694288097324 16-17 0.056272239304298255 18-19 0.06479737222320835 20-21 0.059135157075574034 22-23 0.03556380075873683 24-25 0.010847277333389319 26-27 0.0034673115229895473 28-29 0.008238841141782503 30-31 0.005566784555258448 32-33 0.006743761861227378 34-35 0.007888928969737688 36-37 0.007443586205317011 38-39 0.0043261868543722805 40-41 0.008143410549406644 42-43 0.0043261868543722805 44-45 0.002576625994148196 46-47 0.001813181255141323 48-49 2.544815796689576E-4 50-51 0.0027674871788999143 52-53 0.007825308574820448 54-55 0.014314588856378867 56-57 0.009511249040127292 58-59 0.006298419096806702 60-61 0.002735676981441295 62-63 0.010242883581675546 64-65 0.002894727968734393 66-67 0.023348684934626864 68-69 0.04692004125146406 70-71 0.06126644030530155 72-73 0.0035945523128240267 74-75 0.01510984379284436 76-77 0.022966962565123428 78-79 0.025479968164354386 80-81 0.07605818212355972 82-83 0.029551673439057707 84-85 0.013169421747868558 86-87 0.03378242970105413 88-89 0.048097018557432994 90-91 0.04300738696405384 92-93 0.04205308104029525 94-95 0.014123727671627149 96-97 0.017431988207323598 98-99 0.0403671405749884 100-101 0.07640809429560452 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1571823.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.08966747533413 #Duplication Level Percentage of deduplicated Percentage of total 1 77.33270949958323 45.695640892969855 2 13.316417112189782 15.737253182442867 3 4.223355324765628 7.486699853117483 4 1.8545302719187453 4.383343083624785 5 0.9431559396973926 2.7865385427052614 6 0.531174676130742 1.8832160990330304 7 0.3441429260699412 1.4234703745822925 8 0.23913530812855976 1.1304340671142532 9 0.17371214446406102 0.9238133567527713 >10 0.9319513945268537 10.12887135354741 >50 0.07432832918696647 2.997389463622447 >100 0.03387673003723723 3.4667456152661202 >500 8.63053314794925E-4 0.3331413868356637 >1k 4.315266573974625E-4 0.5476497616351295 >5k 2.1576332869873124E-4 1.0757929667506045 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9710 0.6177540346463947 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7166 0.4559037499769376 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4745 0.301878773882301 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1672 0.1063733003016243 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 6.36203949172394E-5 0.0 0.0 0.0 5 0.0 1.272407898344788E-4 0.0 0.0 0.0 6 0.0 1.272407898344788E-4 0.0 0.0 0.0 7 0.0 1.272407898344788E-4 0.0 0.0 0.0 8 0.0 1.272407898344788E-4 0.0 0.0 0.0 9 0.0 1.272407898344788E-4 0.0 0.0 0.0 10-11 6.36203949172394E-5 1.272407898344788E-4 0.0 0.0 0.0 12-13 6.36203949172394E-5 1.272407898344788E-4 0.0 0.0 0.0 14-15 6.36203949172394E-5 1.272407898344788E-4 0.0 0.0 0.0 16-17 6.36203949172394E-5 1.272407898344788E-4 0.0 3.18101974586197E-5 0.0 18-19 6.36203949172394E-5 1.272407898344788E-4 0.0 9.543059237585911E-5 0.0 20-21 6.36203949172394E-5 1.272407898344788E-4 0.0 1.9086118475171822E-4 0.0 22-23 6.36203949172394E-5 1.272407898344788E-4 0.0 3.18101974586197E-4 0.0 24-25 6.36203949172394E-5 1.272407898344788E-4 0.0 5.725835542551547E-4 0.0 26-27 6.36203949172394E-5 1.272407898344788E-4 0.0 9.861161212172107E-4 0.0 28-29 6.36203949172394E-5 1.272407898344788E-4 0.0 0.001813181255141323 0.0 30-31 6.36203949172394E-5 1.272407898344788E-4 0.0 0.004039895077244702 0.0 32-33 9.543059237585911E-5 1.272407898344788E-4 0.0 0.006648331268851518 0.0 34-35 1.272407898344788E-4 1.272407898344788E-4 0.0 0.008875045090954898 0.0 36-37 1.9086118475171822E-4 1.272407898344788E-4 0.0 0.011610722072396192 0.0 38-39 1.9086118475171822E-4 1.272407898344788E-4 0.0 0.014346399053837486 0.0 40-41 1.9086118475171822E-4 1.272407898344788E-4 0.0 0.017718279984451177 0.0 42-43 1.9086118475171822E-4 1.272407898344788E-4 0.0 0.022044466838823454 0.0 44-45 2.544815796689576E-4 1.272407898344788E-4 0.0 0.029456242846681843 0.0 46-47 4.1353256696205617E-4 1.272407898344788E-4 0.0 0.047810726780305415 0.0 48-49 4.4534276442067584E-4 1.272407898344788E-4 0.0 0.0889413120943007 0.0 50-51 4.4534276442067584E-4 1.272407898344788E-4 0.0 0.11594816973666883 0.0 52-53 4.4534276442067584E-4 1.272407898344788E-4 0.0 0.14362304152566796 0.0 54-55 4.4534276442067584E-4 1.272407898344788E-4 0.0 0.184690006444746 0.0 56-57 4.4534276442067584E-4 1.272407898344788E-4 0.0 0.21172867428457276 0.0 58-59 4.4534276442067584E-4 1.272407898344788E-4 0.0 0.24188474147534422 0.0 60-61 4.4534276442067584E-4 1.272407898344788E-4 0.0 0.2962165587346667 0.0 62-63 5.089631593379152E-4 1.272407898344788E-4 0.0 0.32844028876024844 0.0 64-65 5.089631593379152E-4 1.590509872930985E-4 0.0 0.3628907326079336 0.0 66-67 5.089631593379152E-4 1.9086118475171822E-4 0.0 0.44372044435028624 0.0 68-69 5.725835542551547E-4 1.9086118475171822E-4 0.0 0.5413141301533315 0.0 70-71 5.725835542551547E-4 1.9086118475171822E-4 0.0 0.6210941053795498 0.0 72-73 5.725835542551547E-4 1.9086118475171822E-4 0.0 0.7153159102519813 0.0 74-75 5.725835542551547E-4 1.9086118475171822E-4 0.0 0.7924556390891341 0.0 76-77 5.725835542551547E-4 1.9086118475171822E-4 0.0 0.8449424648958566 0.0 78-79 5.725835542551547E-4 1.9086118475171822E-4 0.0 0.9194737575414026 0.0 80-81 5.725835542551547E-4 1.9086118475171822E-4 0.0 1.0550488191100396 0.0 82-83 5.725835542551547E-4 1.9086118475171822E-4 0.0 1.1799038441351222 0.0 84-85 5.725835542551547E-4 1.9086118475171822E-4 0.0 1.3202822455200107 0.0 86-87 5.725835542551547E-4 1.9086118475171822E-4 0.0 1.6116636542409672 0.0 88-89 5.725835542551547E-4 1.9086118475171822E-4 0.0 1.9635480585282186 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5125 0.0 46.27688 1 GTATCAA 9070 0.0 40.454548 1 GTGCGTA 80 1.073382E-5 35.646645 1 ATCAACG 11770 0.0 30.81392 3 TCAACGC 11870 0.0 30.634413 4 TATCAAC 11975 0.0 30.326109 2 CAACGCA 12010 0.0 30.235806 5 AACGCAG 12360 0.0 29.455585 6 ACGCAGA 13935 0.0 25.951704 7 CGCAGAG 14005 0.0 25.65315 8 GTACATG 9250 0.0 23.995647 1 GCAGAGT 14610 0.0 23.938778 9 TACATGG 9395 0.0 23.374592 2 GAGTACT 8030 0.0 22.312517 12-13 ACATGGG 9255 0.0 22.238747 3 CATGGGG 3170 0.0 21.592457 4 AGTACTT 8440 0.0 21.284924 12-13 GTACTTT 8655 0.0 20.61661 14-15 AGAGTAC 13920 0.0 20.260767 10-11 CAGAGTA 14335 0.0 19.90626 10-11 >>END_MODULE