FastQCFastQC Report
Thu 26 May 2016
SRR936824_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936824_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571823
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109240.6949891940759233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87040.5537519173596519No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56800.3613638431299198No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21290.1354478207788027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59850.047.4693761
GTATCAA103800.038.7212681
ATCAACG134000.029.7387813
TATCAAC135500.029.5156652
TCAACGC135650.029.4120624
CAACGCA137650.028.9847165
AACGCAG140900.028.3197576
CGGTATA908.9506793E-426.388052
ACGCAGA158250.025.0055457
CGCAGAG158550.024.8998938
GTACATG88200.023.3774781
GCAGAGT165900.023.3671889
GAGTACT96350.022.95107512-13
TACATGG90350.022.237812
ACATGGG89150.021.6839833
GTACTTT101000.021.63400714-15
AGTACTT101150.021.5801612-13
GTATAGG4550.020.8831061
AGAGTAC156800.020.13314410-11
CCTATAC4250.020.1163673
Produced by FastQC (version 0.11.5)