##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936824_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1571823 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1588957535295 33.0 31.0 34.0 31.0 34.0 2 32.28179317900298 33.0 31.0 34.0 31.0 34.0 3 32.39977910998885 34.0 31.0 34.0 31.0 34.0 4 36.07705702232376 37.0 35.0 37.0 35.0 37.0 5 35.931025312646526 37.0 35.0 37.0 35.0 37.0 6 36.02160484990995 37.0 35.0 37.0 35.0 37.0 7 35.97512378938341 37.0 35.0 37.0 35.0 37.0 8 35.923905554251334 37.0 35.0 37.0 35.0 37.0 9 37.62496031677867 39.0 37.0 39.0 35.0 39.0 10-11 37.54199327786907 39.0 37.0 39.0 35.0 39.0 12-13 37.4429204178842 39.0 37.0 39.0 34.5 39.0 14-15 38.62391598799611 40.0 38.0 41.0 34.0 41.0 16-17 38.62424458733585 40.0 38.0 41.0 34.5 41.0 18-19 38.784733395554085 40.0 38.0 41.0 35.0 41.0 20-21 38.799443385164864 40.0 38.0 41.0 35.0 41.0 22-23 38.71198506447609 40.0 38.0 41.0 34.0 41.0 24-25 38.619906312606446 40.0 38.0 41.0 34.0 41.0 26-27 38.415449449460915 40.0 38.0 41.0 34.0 41.0 28-29 38.260633035653505 40.0 38.0 41.0 34.0 41.0 30-31 38.19381794260549 40.0 37.5 41.0 34.0 41.0 32-33 38.021107974625636 40.0 37.0 41.0 33.5 41.0 34-35 37.882093912609754 40.0 37.0 41.0 33.0 41.0 36-37 37.708353612334214 40.0 37.0 41.0 33.0 41.0 38-39 37.53503447907302 40.0 37.0 41.0 33.0 41.0 40-41 37.188334818869556 40.0 36.0 41.0 32.0 41.0 42-43 36.95960900177692 40.0 36.0 41.0 31.0 41.0 44-45 36.6709270064123 39.0 36.0 41.0 31.0 41.0 46-47 36.20611830975879 39.0 35.5 41.0 30.0 41.0 48-49 35.76462457923061 39.0 35.0 40.0 28.5 41.0 50-51 35.45937551492757 39.0 35.0 40.0 27.5 41.0 52-53 35.200084551504844 39.0 35.0 40.0 27.0 41.0 54-55 34.81772565995026 38.0 34.0 40.0 26.0 41.0 56-57 34.63650519174233 38.0 34.0 40.0 26.0 41.0 58-59 34.33962698090052 38.0 34.0 40.0 25.0 41.0 60-61 33.977868691322115 37.5 33.5 40.0 23.5 41.0 62-63 33.61542870921217 37.0 33.0 39.5 23.0 41.0 64-65 33.31001614049419 36.5 33.0 39.0 22.0 40.0 66-67 32.988937685731784 36.0 32.5 39.0 21.5 40.0 68-69 32.55349139184246 35.5 32.0 39.0 20.5 40.0 70-71 32.12456523412624 35.0 31.0 38.0 19.0 40.0 72-73 31.720441805470465 35.0 31.0 37.5 18.0 39.5 74-75 31.23771855991419 35.0 31.0 37.0 15.5 39.0 76-77 29.789491564889943 33.5 29.5 35.5 13.0 38.0 78-79 30.456318236849825 34.0 30.0 36.0 11.5 38.0 80-81 30.321669806333155 34.0 30.0 36.0 7.5 37.0 82-83 29.976877485569304 34.0 30.0 35.0 7.0 37.0 84-85 29.53025913223054 34.0 29.5 35.0 2.0 36.5 86-87 29.15600961431408 34.0 29.0 35.0 2.0 36.0 88-89 28.777813723300905 34.0 29.0 35.0 2.0 36.0 90-91 28.329793176458164 33.5 29.0 35.0 2.0 35.0 92-93 27.869162431138875 33.0 27.5 35.0 2.0 35.0 94-95 27.340022381654933 33.0 26.5 34.5 2.0 35.0 96-97 26.599510250199927 32.5 25.0 34.0 2.0 35.0 98-99 25.764818939537086 32.0 24.5 34.0 2.0 35.0 100-101 22.61036039045109 28.5 9.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15066810499450156 1101 2 0.20832727887473368 1101 3 0.22222189991302344 1101 4 0.1278214613502371 1101 5 0.049311448497341814 1101 6 0.06284062148893099 1101 7 0.08805580127823731 1101 8 0.15057875787304908 1101 9 0.220101440908806 1101 10-11 0.3053394974943373 1101 12-13 0.2683517512277547 1101 14-15 0.3123727027533576 1101 16-17 0.393342054411562 1101 18-19 0.2635060874061068 1101 20-21 0.12172245700801909 1101 22-23 0.11891296762855319 1101 24-25 0.1532175962091884 1101 26-27 0.2721931540598277 1101 28-29 0.35275905434426846 1101 30-31 0.2918351632423821 1101 32-33 0.3943018464338053 1101 34-35 0.19318137065177154 1101 36-37 0.3157354502550689 1101 38-39 0.2493297340997458 1101 40-41 0.41302338550976714 1101 42-43 0.3835797730313004 1101 44-45 0.19510137867240473 1101 46-47 0.02271611598377632 1101 48-49 0.05866527740266747 1101 50-51 0.0629770150731801 1101 52-53 0.028588618314259406 1101 54-55 -0.1152113184081216 1101 56-57 0.10228618373263743 1101 58-59 0.07731463422411622 1101 60-61 0.14783308453031196 1101 62-63 0.18339217533021923 1101 64-65 0.10708033720363375 1101 66-67 0.1891797671694846 1101 68-69 -0.08179978202034022 1101 70-71 -0.016543575263774812 1101 72-73 -0.022755576270693467 1101 74-75 0.15857611769808244 1101 76-77 0.1587144252294017 1101 78-79 0.13771103811796692 1101 80-81 0.1072388669792872 1101 82-83 0.42740741516507086 1101 84-85 0.22528540259774843 1101 86-87 -0.12096573733585103 1101 88-89 -0.05140347936329803 1101 90-91 0.2782837536772611 1101 92-93 0.18409539746701853 1101 94-95 0.0723220348935989 1101 96-97 0.19371827130278874 1101 98-99 0.27988113095087996 1101 100-101 0.592871189426976 1102 1 -0.0727322878262413 1102 2 -0.012260741988214363 1102 3 0.08938608119382252 1102 4 0.1442640860460358 1102 5 0.013990389718586016 1102 6 0.1388996991621596 1102 7 0.19037109606148306 1102 8 0.1586086904052877 1102 9 0.2305383847226068 1102 10-11 0.1933321820464542 1102 12-13 0.22984558551689815 1102 14-15 0.16155574788691496 1102 16-17 0.15155917463016522 1102 18-19 0.1571098458869642 1102 20-21 0.17453089200959937 1102 22-23 0.18373640972394867 1102 24-25 0.10312274472721583 1102 26-27 0.15106431206445592 1102 28-29 0.1120353251426991 1102 30-31 0.11816691158630732 1102 32-33 -0.053855366434504504 1102 34-35 0.3221624353505419 1102 36-37 0.3307676968426705 1102 38-39 0.40327984994833344 1102 40-41 0.45613141558811066 1102 42-43 0.39096648711641535 1102 44-45 0.26773093004895543 1102 46-47 0.07666517468670264 1102 48-49 0.2102338992784425 1102 50-51 0.1230726466557357 1102 52-53 0.0722436301324123 1102 54-55 0.2693966774041243 1102 56-57 -0.026151362289645874 1102 58-59 0.021501536116623754 1102 60-61 0.12001830665483482 1102 62-63 0.2215494829771032 1102 64-65 0.29819021619326946 1102 66-67 0.060672595347590175 1102 68-69 0.14849338269461754 1102 70-71 0.017006035487902693 1102 72-73 0.005878671748799746 1102 74-75 0.028568892654373457 1102 76-77 -0.0636177036869583 1102 78-79 -0.4169408401746786 1102 80-81 0.0565882849659296 1102 82-83 0.18280450823326078 1102 84-85 0.22705812649025958 1102 86-87 0.17772074096933466 1102 88-89 0.03479635907568479 1102 90-91 0.13451905388475183 1102 92-93 0.16544771276180015 1102 94-95 0.24013242379196598 1102 96-97 0.38441677482198955 1102 98-99 0.3584640527264753 1102 100-101 0.5662158087014362 1103 1 0.11707838502885437 1103 2 0.18650988136673163 1103 3 0.14666638671792498 1103 4 0.07628538313025501 1103 5 -0.024271832679687577 1103 6 -0.017569399440667155 1103 7 0.11427656771294892 1103 8 0.08217439488775113 1103 9 0.18683613140040478 1103 10-11 0.1459141734986602 1103 12-13 0.20958880349102316 1103 14-15 0.17755297647953938 1103 16-17 0.14473668402413864 1103 18-19 0.07757018088714318 1103 20-21 0.04286035757462514 1103 22-23 0.08229729941538011 1103 24-25 0.13469001726077323 1103 26-27 0.1255790425932375 1103 28-29 0.13323201584462652 1103 30-31 0.1676749150666197 1103 32-33 0.05048730190220141 1103 34-35 0.06345637117244962 1103 36-37 0.06710325158172026 1103 38-39 0.15675008497842668 1103 40-41 0.29035969678283635 1103 42-43 0.045672040448586415 1103 44-45 0.06118322400662635 1103 46-47 0.24652314859532254 1103 48-49 0.19235309711375947 1103 50-51 -0.06013851846906704 1103 52-53 -0.13758154856120797 1103 54-55 0.16935436870840448 1103 56-57 -0.04611959832778467 1103 58-59 -0.2242831314032756 1103 60-61 -0.31920554220033637 1103 62-63 0.03848924959974198 1103 64-65 -0.0176131231545682 1103 66-67 -0.027790994587924445 1103 68-69 -0.09667765507384019 1103 70-71 -0.09072696283613624 1103 72-73 0.06691673386711727 1103 74-75 -0.1918346551295258 1103 76-77 0.04611257923152934 1103 78-79 -0.027365170099184155 1103 80-81 -0.06101366908799122 1103 82-83 0.22739442462663106 1103 84-85 -0.025737867057234354 1103 86-87 -0.09481504214789283 1103 88-89 0.15234640899926433 1103 90-91 0.006173627799647363 1103 92-93 -0.0875005784312961 1103 94-95 -0.05839366964366732 1103 96-97 0.1901372246395745 1103 98-99 0.004244144487511647 1103 100-101 0.41732471312817765 1104 1 0.0024373762389089393 1104 2 0.11335994571679464 1104 3 0.056004676375359 1104 4 0.004911920001468673 1104 5 -0.15461186115800984 1104 6 0.06186938584918522 1104 7 0.08716020362849974 1104 8 0.0754450211371065 1104 9 0.07662055868483009 1104 10-11 0.1525025247678613 1104 12-13 0.14720928871788885 1104 14-15 0.2482031859595324 1104 16-17 0.1837069803082585 1104 18-19 0.18579791850318372 1104 20-21 0.154205137004503 1104 22-23 0.13999842759522352 1104 24-25 0.06074631672133535 1104 26-27 0.09312898235062761 1104 28-29 0.10095908942275855 1104 30-31 0.11915308396499569 1104 32-33 1.4022229980525935E-4 1104 34-35 0.10084273169710656 1104 36-37 0.03527581244045308 1104 38-39 0.02926319459260185 1104 40-41 0.08172241208172437 1104 42-43 0.023248610179408047 1104 44-45 0.09486021406659972 1104 46-47 0.02718971942146453 1104 48-49 0.1120832375885854 1104 50-51 0.1016702317705338 1104 52-53 -0.07314009342826466 1104 54-55 0.10781003562151881 1104 56-57 0.03967546725451854 1104 58-59 -0.26448138436748536 1104 60-61 -0.1433611187495316 1104 62-63 0.0637937143988907 1104 64-65 -0.04191554107401174 1104 66-67 -0.15075215863951286 1104 68-69 -0.061945905847402116 1104 70-71 0.006734290210225424 1104 72-73 0.020206802050804384 1104 74-75 -0.11684098744968807 1104 76-77 -0.08654285355688174 1104 78-79 0.04245430743305789 1104 80-81 -0.04180414801676946 1104 82-83 0.10769988205974812 1104 84-85 -0.20826169027254693 1104 86-87 -0.2713998336262975 1104 88-89 -0.24588738710687608 1104 90-91 -0.1850559376320433 1104 92-93 -0.1251203790830111 1104 94-95 -0.0062685772260202555 1104 96-97 0.2753086422578015 1104 98-99 -0.008668483185751086 1104 100-101 0.8604981297058494 1105 1 0.02696899216396531 1105 2 0.105270562422497 1105 3 0.11016158504046558 1105 4 0.126506377456586 1105 5 -0.0032750149441156395 1105 6 0.16903175899516043 1105 7 0.1636531778673742 1105 8 0.1265769493182134 1105 9 0.17813304892682424 1105 10-11 0.12018402359689873 1105 12-13 0.19440874226980043 1105 14-15 0.1853226238986423 1105 16-17 0.005745426834408818 1105 18-19 0.04137461639545137 1105 20-21 0.2092988138195011 1105 22-23 0.11574979371544458 1105 24-25 0.12516864302890696 1105 26-27 0.09982785042244302 1105 28-29 0.09104495983649485 1105 30-31 0.06726491066289242 1105 32-33 0.16495286866982184 1105 34-35 0.16519675199375428 1105 36-37 0.022546499403766518 1105 38-39 -0.047195626640814226 1105 40-41 0.10311195150404728 1105 42-43 0.05320079597176175 1105 44-45 -0.023523860874860247 1105 46-47 0.07005662028016246 1105 48-49 0.2988513874714869 1105 50-51 0.23855059867140227 1105 52-53 0.4697658394366826 1105 54-55 0.49732018394315247 1105 56-57 0.33682613079862733 1105 58-59 0.3149487483413367 1105 60-61 0.12286643784687357 1105 62-63 0.2746397331342578 1105 64-65 0.3920227883561225 1105 66-67 0.2771020568171636 1105 68-69 0.28650745090512686 1105 70-71 0.06386738935152181 1105 72-73 0.2737782306222307 1105 74-75 0.17448902816311573 1105 76-77 0.2165845859435045 1105 78-79 0.359579600173106 1105 80-81 0.31788554753358156 1105 82-83 0.36111518260619846 1105 84-85 0.1506668811560239 1105 86-87 0.22727307816136744 1105 88-89 0.34146624286970706 1105 90-91 0.24096865253189037 1105 92-93 0.4432149058506347 1105 94-95 0.5256839988863895 1105 96-97 0.7607692199314933 1105 98-99 0.9627942412482824 1105 100-101 0.972324827598122 1106 1 -0.0237621875560734 1106 2 0.07183413691999618 1106 3 0.102578649476051 1106 4 0.08756822679900012 1106 5 0.014594790968487814 1106 6 0.1124964305601992 1106 7 0.0917449001025048 1106 8 0.2177569077237962 1106 9 0.2017250661366674 1106 10-11 0.22410376068207682 1106 12-13 0.32004189504286273 1106 14-15 0.21937730446189363 1106 16-17 0.2881576346157857 1106 18-19 0.2553839781178695 1106 20-21 0.17667878295143424 1106 22-23 0.15515200811140772 1106 24-25 0.18175685834255972 1106 26-27 0.33377556541132947 1106 28-29 0.3101739203933107 1106 30-31 0.198885454012256 1106 32-33 0.25940957889740446 1106 34-35 0.20614534892720826 1106 36-37 0.2980461686745528 1106 38-39 0.30420639706443353 1106 40-41 0.43564825722894085 1106 42-43 0.39322543678791533 1106 44-45 -0.03623756855790816 1106 46-47 -0.07017033146912155 1106 48-49 -0.3349360281329723 1106 50-51 -0.2819970710284494 1106 52-53 -0.15348814868089988 1106 54-55 -0.06650385023816341 1106 56-57 -0.288241653792376 1106 58-59 -0.29060824732641066 1106 60-61 -0.3859410896691955 1106 62-63 -0.4714093935618493 1106 64-65 -0.48239173155020154 1106 66-67 -0.3820248776507853 1106 68-69 -0.31783240166711124 1106 70-71 -0.4140899555186053 1106 72-73 -0.22185971921419068 1106 74-75 -0.4253628885765508 1106 76-77 -0.18950768452743816 1106 78-79 -0.26239392957166885 1106 80-81 -0.12908604550858982 1106 82-83 -0.04265453451603918 1106 84-85 -0.1485424972518281 1106 86-87 -0.09271935380078133 1106 88-89 0.035519374070879906 1106 90-91 -0.1932847635135424 1106 92-93 -0.04722597704738618 1106 94-95 0.08001595567110797 1106 96-97 0.1042344874050194 1106 98-99 0.3438874055819703 1106 100-101 0.6537096891387826 1107 1 0.23284821619868978 1107 2 0.3195008377741928 1107 3 0.2973560153754491 1107 4 0.20304394632447753 1107 5 0.1569101660789869 1107 6 0.2520279636757081 1107 7 0.26095487504792914 1107 8 0.21466904087935745 1107 9 0.29413152660366393 1107 10-11 0.25917467539394323 1107 12-13 0.23515486042066414 1107 14-15 0.33577059296072065 1107 16-17 0.305430885801357 1107 18-19 0.34700290433628567 1107 20-21 0.2705290562075078 1107 22-23 0.24546753462383464 1107 24-25 0.35387765754941114 1107 26-27 0.44863407786291987 1107 28-29 0.42720212022432946 1107 30-31 0.3430500083777517 1107 32-33 0.38140107596948525 1107 34-35 0.3376183920572231 1107 36-37 0.432960553323376 1107 38-39 0.3236084326878341 1107 40-41 0.48230508276996176 1107 42-43 0.3739015440977056 1107 44-45 0.3379230054019402 1107 46-47 0.30005440272280737 1107 48-49 0.5721074584093486 1107 50-51 0.46301460157220475 1107 52-53 0.3325438020109104 1107 54-55 0.6865699752979708 1107 56-57 0.4726934729212786 1107 58-59 0.4461483569724294 1107 60-61 0.34534306733949194 1107 62-63 0.4111539876828658 1107 64-65 0.491536611380063 1107 66-67 0.5140596730029259 1107 68-69 0.442143642639941 1107 70-71 0.48397014616488576 1107 72-73 0.4980607459316211 1107 74-75 0.6136743904865156 1107 76-77 0.35589213867417513 1107 78-79 0.6825226284274848 1107 80-81 0.553914936162009 1107 82-83 0.675963751907112 1107 84-85 0.6682269762113187 1107 86-87 0.8818585522616438 1107 88-89 0.8654278491603762 1107 90-91 0.671514531246487 1107 92-93 0.8911864027468006 1107 94-95 1.0155010279289023 1107 96-97 0.8072412809562763 1107 98-99 0.9567203066872061 1107 100-101 1.3000146272837654 1108 1 0.08573853454006297 1108 2 0.11776722566755637 1108 3 0.0860743423145891 1108 4 0.003254905844457312 1108 5 -0.0811135958119209 1108 6 -0.057722410916525746 1108 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2316 92-93 -1.732710130700628 2316 94-95 -1.8844715694435727 2316 96-97 -1.6888838913617832 2316 98-99 -1.8561714276948003 2316 100-101 -2.563354629932757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 8.0 5 32.0 6 83.0 7 165.0 8 445.0 9 918.0 10 1630.0 11 2736.0 12 4266.0 13 6344.0 14 7842.0 15 11055.0 16 15073.0 17 21100.0 18 18425.0 19 10201.0 20 8179.0 21 7979.0 22 8500.0 23 9360.0 24 10357.0 25 12731.0 26 15308.0 27 19341.0 28 24130.0 29 30045.0 30 37564.0 31 47697.0 32 60334.0 33 79902.0 34 109145.0 35 155419.0 36 237389.0 37 351161.0 38 234096.0 39 12860.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.89628665697314 20.099523065615863 15.387449530542572 21.61674074686843 2 15.364236556690654 21.531626506982267 40.220465247075424 22.88367168925165 3 18.690359780153084 26.53024740094451 31.02948745436026 23.749905364542144 4 12.498162961096765 18.007752781324616 38.24832694266466 31.24575731491396 5 12.783514337156998 38.545352750685524 34.24515997684169 14.425972935315787 6 29.6265546438753 37.9155922772475 17.947058924573568 14.510794154303634 7 26.63837248436366 34.531774969000885 21.423522682955227 17.406329863680234 8 26.207912722997435 34.529396757777434 20.28218189961592 18.980508619609203 9 26.866545236385058 16.403967118393254 20.973825900022966 35.75566174519872 10-11 24.050163408984346 27.613287246719253 27.69529393576758 20.64125540852882 12-13 25.6672828672382 25.33796390407122 28.324516607034866 20.670236621655715 14-15 22.783812706136246 25.949149242640175 27.662720387637936 23.60431766358565 16-17 20.78443310729007 29.320127011756412 28.120691706381702 21.77474817457182 18-19 20.698498455129403 27.121489208809308 31.811641895401777 20.368370440659504 20-21 21.84663090730836 26.204015388696927 31.87775440051966 20.071599303475054 22-23 21.422511487643924 26.331222692490602 31.646570248393957 20.599695571471514 24-25 21.05918620597076 26.459900859550185 32.00770703593086 20.4732058985482 26-27 21.001388519977667 26.63028627687375 31.747896940961656 20.620428262186927 28-29 20.713388893077237 26.582026012495696 32.016071799501404 20.688513294925663 30-31 21.043399924800692 26.48720625668412 31.885364955214424 20.584028863300766 32-33 20.874865641293827 26.591174489929976 31.652954841796298 20.881005026979896 34-35 21.18101083900668 26.628570774190223 31.598882316902095 20.591536069901 36-37 21.282178850885067 26.41994756333658 31.52014790676428 20.777725679014072 38-39 21.06167837302509 26.674240433479913 31.558333805235634 20.70574738825937 40-41 21.226052274799294 26.591875984932013 31.374581771703813 20.807489968564884 42-43 21.090860739408953 26.670242132861016 31.16709069659879 21.07180643113124 44-45 21.245729445670914 26.986785934686765 30.773344106475975 20.99414051316635 46-47 21.44151365932486 27.15762619129417 30.13105826366887 21.269801885712106 48-49 21.520109961665472 27.381307216695543 29.7626223859531 21.33596043568588 50-51 21.741536987477062 27.580655371516205 29.36546134659375 21.312346294412983 52-53 21.71819510910296 27.714818127798512 29.2342688530097 21.332717910088828 54-55 21.577843052302963 27.656167392893476 29.289684652788512 21.47630490201505 56-57 21.611419122870025 28.01638483299132 28.92927591240597 21.44292013173268 58-59 21.658809532898168 27.666622132305225 29.153815322365155 21.52075301243145 60-61 21.739073728691977 27.652498271881885 29.33579505998391 21.272632939442225 62-63 21.706388060233245 27.55924172123706 29.471098208895025 21.263272009634672 64-65 21.639720769997883 27.526454163876647 29.319627375228436 21.51419769089703 66-67 21.58045148849457 27.852340880620787 29.001675124998172 21.565532505886477 68-69 21.754562539524194 27.734660794924615 29.09407044818052 21.416706217370667 70-71 21.67041180392616 27.719688564055843 28.960482651913455 21.64941698010454 72-73 21.658384858048176 27.80686828663742 28.81926252935692 21.715484325957483 74-75 21.720738228154453 27.898056388558146 28.650764844297 21.730440538990408 76-77 21.66132679145603 27.968147792816232 28.69357980738277 21.676945608344965 78-79 21.641282080645365 27.895306078304134 28.58546143960504 21.87795040144546 80-81 21.55283257147038 28.198981425812995 28.47687849319731 21.77130750951931 82-83 21.73096627066114 28.187774502384656 28.310975514713345 21.77028371224086 84-85 21.66913571135685 28.03997084862503 28.210921840659296 22.07997159935882 86-87 21.46469201119595 27.947374331069398 28.4237652120776 22.164168445657054 88-89 21.799909721080127 27.73586004031695 28.41711914813346 22.047111090469464 90-91 21.486093756759715 27.860043058584615 28.595213676953733 22.05864950770194 92-93 21.454005953596557 27.714189193057997 28.57875854978582 22.253046303559625 94-95 21.232988701654065 27.671786199845656 28.630259259471323 22.46496583902895 96-97 21.427817070540563 27.555176518415927 28.541486010793864 22.475520400249646 98-99 21.1134212530371 27.577485362444516 28.54910221508538 22.759991169433004 100-101 21.15890587965465 27.941441715127514 28.331376449252655 22.568275955965177 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 734.0 1 531.0 2 387.0 3 445.0 4 393.5 5 410.0 6 682.0 7 1384.0 8 2356.0 9 2887.5 10 2644.5 11 2317.5 12 2383.5 13 2603.5 14 2835.5 15 2994.5 16 3179.0 17 3431.5 18 3672.5 19 3986.0 20 4301.0 21 4879.5 22 5650.5 23 6647.5 24 7858.0 25 9295.5 26 11551.0 27 14471.0 28 16649.0 29 18994.0 30 22251.0 31 25391.0 32 29140.0 33 32810.0 34 36921.5 35 40717.5 36 45242.5 37 49884.0 38 51444.0 39 51652.5 40 51516.0 41 51836.0 42 52127.0 43 53444.0 44 55520.5 45 56932.0 46 56948.0 47 56156.5 48 58575.5 49 57435.0 50 52887.5 51 50329.5 52 47518.0 53 45442.0 54 43014.0 55 39811.0 56 36054.5 57 32165.5 58 29546.0 59 26726.5 60 22741.5 61 18328.0 62 14549.0 63 11060.0 64 7585.0 65 5315.5 66 3698.0 67 2578.5 68 1804.5 69 1169.5 70 838.5 71 639.5 72 497.0 73 427.5 74 328.0 75 245.0 76 182.5 77 96.0 78 46.0 79 23.5 80 17.0 81 10.5 82 5.5 83 4.5 84 1.5 85 2.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020867489532854527 2 0.0035627421153654067 3 1.272407898344788E-4 4 0.0 5 0.0020994730322689004 6 0.0 7 2.544815796689576E-4 8 0.0 9 1.272407898344788E-4 10-11 0.0 12-13 0.006012127319679124 14-15 0.002990158561110252 16-17 0.0 18-19 5.407733567965351E-4 20-21 0.0012724078983447882 22-23 0.001622320070389605 24-25 7.952549364654925E-4 26-27 3.4991217204481673E-4 28-29 9.543059237585911E-5 30-31 0.0 32-33 2.8629177712757734E-4 34-35 0.0 36-37 0.001399648688179267 38-39 0.0108154671359307 40-41 8.906855288413517E-4 42-43 0.0 44-45 8.270651339241123E-4 46-47 1.9086118475171822E-4 48-49 1.5905098729309853E-4 50-51 0.0017495608602240836 52-53 3.1810197458619706E-4 54-55 0.0 56-57 2.2267138221033792E-4 58-59 1.9086118475171822E-4 60-61 0.0016859404653068444 62-63 0.0 64-65 3.18101974586197E-5 66-67 0.0 68-69 6.36203949172394E-5 70-71 4.4534276442067584E-4 72-73 2.8629177712757734E-4 74-75 0.002067662834810281 76-77 6.36203949172394E-5 78-79 1.9086118475171822E-4 80-81 0.0011451671085103093 82-83 9.543059237585911E-5 84-85 0.0017495608602240836 86-87 2.8629177712757734E-4 88-89 0.0017495608602240836 90-91 6.998243440896335E-4 92-93 0.0 94-95 0.0 96-97 2.544815796689576E-4 98-99 6.362039491723941E-4 100-101 0.0013678384907206474 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1571823.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.658193578468676 #Duplication Level Percentage of deduplicated Percentage of total 1 76.26611360949015 43.97366341973467 2 13.960803316939987 16.099094003181065 3 4.474260555828719 7.739333437454373 4 1.872254568799036 4.318032654239488 5 0.9460390216226175 2.7273450520750995 6 0.5792503352310465 2.003911677548673 7 0.37289280152713256 1.5050227734128234 8 0.2559380817816069 1.1805541970772668 9 0.18612469290603242 0.9658452215978146 >10 0.9711213775469829 10.321869501528012 >50 0.07678584136766577 3.0467928671836897 >100 0.03631244524529355 3.643303050811527 >500 0.001439135383058829 0.561250064301805 >1k 3.3210816532126824E-4 0.2985929490280728 >5k 2.2140544354751215E-4 0.9181190634501494 >10k+ 1.1070272177375608E-4 0.6972700673755167 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10924 0.6949891940759233 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8704 0.5537519173596519 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5680 0.3613638431299198 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2129 0.1354478207788027 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 6.36203949172394E-5 0.0 0.0 0.0 0.0 9 6.36203949172394E-5 0.0 0.0 0.0 0.0 10-11 6.36203949172394E-5 0.0 0.0 0.0 0.0 12-13 9.543059237585911E-5 1.272407898344788E-4 0.0 0.0 0.0 14-15 1.272407898344788E-4 1.272407898344788E-4 0.0 0.0 0.0 16-17 1.272407898344788E-4 1.272407898344788E-4 0.0 3.18101974586197E-5 0.0 18-19 1.272407898344788E-4 1.272407898344788E-4 0.0 9.543059237585911E-5 0.0 20-21 1.272407898344788E-4 2.544815796689576E-4 0.0 1.9086118475171822E-4 0.0 22-23 1.272407898344788E-4 2.544815796689576E-4 0.0 3.8172236950343645E-4 0.0 24-25 1.9086118475171822E-4 2.544815796689576E-4 0.0 6.362039491723941E-4 0.0 26-27 1.9086118475171822E-4 2.544815796689576E-4 0.0 0.0010497365161344502 0.0 28-29 3.1810197458619706E-4 2.544815796689576E-4 0.0 0.0018768016500585625 0.0 30-31 3.1810197458619706E-4 2.544815796689576E-4 0.0 0.004167135867079182 0.0 32-33 3.8172236950343645E-4 2.544815796689576E-4 0.0 0.006711951663768758 0.0 34-35 4.771529618792955E-4 2.544815796689576E-4 0.0 0.009065906275706616 0.0 36-37 5.725835542551547E-4 2.544815796689576E-4 0.0 0.01176977305968929 0.0 38-39 5.725835542551547E-4 3.1810197458619706E-4 0.0 0.014346399053837486 0.0 40-41 5.725835542551547E-4 3.1810197458619706E-4 0.0 0.017495608602240836 0.0 42-43 5.725835542551547E-4 3.1810197458619706E-4 0.0 0.021726364864237256 0.0 44-45 5.725835542551547E-4 3.1810197458619706E-4 0.0 0.029074520477178407 0.0 46-47 6.043937517137744E-4 3.1810197458619706E-4 0.0 0.04717452283113302 0.0 48-49 6.362039491723941E-4 3.1810197458619706E-4 0.0 0.0881460571578352 0.0 50-51 6.362039491723941E-4 3.1810197458619706E-4 0.0 0.11534377598495504 0.0 52-53 6.362039491723941E-4 3.1810197458619706E-4 0.0 0.14260511520699215 0.0 54-55 6.362039491723941E-4 3.1810197458619706E-4 0.0 0.1838947515082805 0.0 56-57 6.998243440896335E-4 3.1810197458619706E-4 0.0 0.2109652295455659 0.0 58-59 6.998243440896335E-4 3.1810197458619706E-4 0.0 0.24140758851346494 0.0 60-61 6.998243440896335E-4 3.1810197458619706E-4 0.0 0.29586664656262185 0.0 62-63 6.998243440896335E-4 3.1810197458619706E-4 0.0 0.32805856639074504 0.0 64-65 7.634447390068729E-4 4.4534276442067584E-4 0.0 0.3630497835952267 0.0 66-67 7.634447390068729E-4 4.4534276442067584E-4 0.0 0.44330691178332415 0.0 68-69 7.634447390068729E-4 4.4534276442067584E-4 0.0 0.5410596485736625 0.0 70-71 7.634447390068729E-4 4.4534276442067584E-4 0.0 0.6211259155770084 0.0 72-73 7.634447390068729E-4 4.4534276442067584E-4 0.0 0.7149659980799365 0.0 74-75 7.634447390068729E-4 4.4534276442067584E-4 0.0 0.7913422821780824 0.0 76-77 7.634447390068729E-4 4.4534276442067584E-4 0.0 0.8434791958127601 0.0 78-79 7.634447390068729E-4 4.4534276442067584E-4 0.0 0.9187421229998542 0.0 80-81 7.634447390068729E-4 4.4534276442067584E-4 0.0 1.0553351108871674 0.0 82-83 7.634447390068729E-4 4.4534276442067584E-4 0.0 1.1800947053198738 0.0 84-85 7.634447390068729E-4 4.4534276442067584E-4 0.0 1.3204094863098454 0.0 86-87 7.634447390068729E-4 4.4534276442067584E-4 0.0 1.6118863256231777 0.0 88-89 7.634447390068729E-4 4.4534276442067584E-4 0.0 1.961416775298491 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5985 0.0 47.469376 1 GTATCAA 10380 0.0 38.721268 1 ATCAACG 13400 0.0 29.738781 3 TATCAAC 13550 0.0 29.515665 2 TCAACGC 13565 0.0 29.412062 4 CAACGCA 13765 0.0 28.984716 5 AACGCAG 14090 0.0 28.319757 6 CGGTATA 90 8.9506793E-4 26.38805 2 ACGCAGA 15825 0.0 25.005545 7 CGCAGAG 15855 0.0 24.899893 8 GTACATG 8820 0.0 23.377478 1 GCAGAGT 16590 0.0 23.367188 9 GAGTACT 9635 0.0 22.951075 12-13 TACATGG 9035 0.0 22.23781 2 ACATGGG 8915 0.0 21.683983 3 GTACTTT 10100 0.0 21.634007 14-15 AGTACTT 10115 0.0 21.58016 12-13 GTATAGG 455 0.0 20.883106 1 AGAGTAC 15680 0.0 20.133144 10-11 CCTATAC 425 0.0 20.116367 3 >>END_MODULE