FastQCFastQC Report
Thu 26 May 2016
SRR936823_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936823_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073490
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165801.5444950581747385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118501.1038761423022105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80080.7459780715237216No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34950.32557359640052536No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA32230.30023567988523414No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA26300.2449952957177058No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24740.23046325536334758No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21620.20139917465463114No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19170.1785764189699019No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT18040.16805000512347576No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13840.12892528109251133No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT11710.10908345676252224No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG11550.10759299108515216No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11520.10731352877064529No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10900.10153797427083626No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC405.36574E-447.5399321
CGTCACT1703.6379788E-1233.55761
GGTATCA114300.030.6950741
GTATCAA169700.030.6194131
AATCGAC350.008390279527.14476820-21
GTACATG123800.026.8036141
TACATGG124750.026.4482982
GAGTACT99050.025.37975712-13
TATCAAC204200.025.354262
ATCAACG206600.025.0815723
ATGGGAT33800.025.0346535
ACATGGG124950.024.9969863
TCAACGC208000.024.9367734
CAACGCA211000.024.6024555
AACGCAG214000.024.257566
CATGGGG23400.023.7710744
CATGGGA98700.023.4579814
GTACTTT106050.023.43511814-15
AGTACTT100000.022.9289212-13
ACGCAGA232450.022.2299397
Produced by FastQC (version 0.11.5)