##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936823_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1073490 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80267072818564 33.0 31.0 34.0 30.0 34.0 2 31.84125236378541 33.0 31.0 34.0 30.0 34.0 3 31.811672209335903 33.0 31.0 34.0 30.0 34.0 4 35.43047070769173 37.0 35.0 37.0 33.0 37.0 5 35.485873180001676 37.0 35.0 37.0 33.0 37.0 6 35.58608370827861 37.0 35.0 37.0 33.0 37.0 7 35.53689088859701 37.0 35.0 37.0 33.0 37.0 8 35.51597499743826 37.0 35.0 37.0 33.0 37.0 9 37.09314758404829 39.0 37.0 39.0 33.0 39.0 10-11 36.97613298680007 39.0 37.0 39.0 33.0 39.0 12-13 36.86008346607793 39.0 37.0 39.0 33.0 39.0 14-15 37.866404437861554 40.0 37.5 41.0 32.5 41.0 16-17 37.845937083717594 40.0 37.5 41.0 33.0 41.0 18-19 37.948979496781526 40.0 38.0 41.0 33.0 41.0 20-21 37.97058007061081 40.0 38.0 41.0 33.0 41.0 22-23 37.834241120084954 40.0 37.5 41.0 33.0 41.0 24-25 37.659732740873224 40.0 37.0 41.0 32.0 41.0 26-27 37.56697686983577 40.0 37.0 41.0 32.0 41.0 28-29 37.58139805680537 40.0 37.0 41.0 32.0 41.0 30-31 37.328677025403124 39.0 36.5 41.0 32.0 41.0 32-33 37.01134756728055 39.0 35.0 41.0 31.5 41.0 34-35 36.7619456166336 39.0 35.0 41.0 31.0 41.0 36-37 36.54618301055436 39.0 35.0 41.0 31.0 41.0 38-39 36.32367930767869 39.0 35.0 41.0 30.0 41.0 40-41 36.04096218874885 39.0 35.0 40.5 30.0 41.0 42-43 35.717213947032576 38.0 35.0 40.0 29.5 41.0 44-45 35.26461774213081 38.0 35.0 40.0 26.5 41.0 46-47 34.635953758302364 38.0 34.5 40.0 23.5 41.0 48-49 34.058374554024724 38.0 34.0 40.0 20.0 41.0 50-51 33.30714072790617 37.5 33.0 39.5 16.5 40.5 52-53 33.521408676373326 38.0 33.5 40.0 15.0 40.5 54-55 33.792844367437056 38.0 34.0 40.0 14.5 41.0 56-57 33.68669014150109 38.0 34.0 40.0 12.5 41.0 58-59 33.44607867795695 38.0 34.0 40.0 9.0 41.0 60-61 33.16595124314153 37.5 33.0 40.0 7.5 41.0 62-63 32.86075184678013 37.0 33.0 40.0 6.5 41.0 64-65 32.5342457777902 36.5 33.0 40.0 2.0 41.0 66-67 32.168586106996806 36.0 32.0 39.0 2.0 41.0 68-69 31.7389211823119 35.5 32.0 39.0 2.0 41.0 70-71 31.38724580573643 35.0 31.5 38.5 2.0 40.0 72-73 31.020516260049 35.0 31.0 37.5 2.0 40.0 74-75 30.59940614258167 35.0 31.0 37.0 2.0 39.0 76-77 30.162558570643412 35.0 30.5 36.5 2.0 39.0 78-79 29.749858871531174 35.0 30.0 36.0 2.0 39.0 80-81 29.357492850422453 34.5 29.5 36.0 2.0 37.5 82-83 28.959028030070144 34.0 29.0 35.0 2.0 37.0 84-85 28.615493856486786 34.0 29.0 35.0 2.0 36.5 86-87 28.274995109409495 34.0 29.0 35.0 2.0 36.0 88-89 27.895872341614734 34.0 28.0 35.0 2.0 36.0 90-91 27.548688390203914 34.0 27.0 35.0 2.0 35.5 92-93 27.217565138007807 34.0 26.0 35.0 2.0 35.0 94-95 26.835434889938426 34.0 25.5 35.0 2.0 35.0 96-97 26.409169158538973 34.0 25.0 35.0 2.0 35.0 98-99 25.844031150732654 33.0 23.5 35.0 2.0 35.0 100-101 23.916038342229548 31.0 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21638556658676222 1101 2 0.1781518131403601 1101 3 0.3486819443946203 1101 4 0.19346613159014225 1101 5 0.2211711344618834 1101 6 0.0801973729984411 1101 7 0.18748182068318897 1101 8 0.07295166790729013 1101 9 0.20453199478627226 1101 10-11 0.2096483961719926 1101 12-13 0.3133552609387422 1101 14-15 0.5801366190748283 1101 16-17 0.51921037132594 1101 18-19 0.41733334855702253 1101 20-21 0.5081504961869214 1101 22-23 0.6230755676378266 1101 24-25 0.4822416845528963 1101 26-27 0.5629276353812926 1101 28-29 0.4952833065621647 1101 30-31 0.48657713251754586 1101 32-33 0.5541321659185954 1101 34-35 0.6854689090016919 1101 36-37 0.7783971228538391 1101 38-39 0.823152826301623 1101 40-41 0.7455413124222403 1101 42-43 0.8781856518860707 1101 44-45 0.7983621243917796 1101 46-47 0.8969123962725476 1101 48-49 0.49403986016860557 1101 50-51 0.25241722386842724 1101 52-53 0.48427023924617174 1101 54-55 0.5339798117069492 1101 56-57 0.3631371306444038 1101 58-59 0.5722530434457482 1101 60-61 0.742959007846899 1101 62-63 0.7064405910428135 1101 64-65 0.5954210204211918 1101 66-67 0.5355893858003853 1101 68-69 0.5182542986449654 1101 70-71 0.3070253857831027 1101 72-73 0.39421429402472086 1101 74-75 0.3381984530859192 1101 76-77 0.1802474319466043 1101 78-79 0.29952530154860213 1101 80-81 0.33018121656699506 1101 82-83 0.22119239899458165 1101 84-85 0.27331993348881767 1101 86-87 0.3933910096683526 1101 88-89 0.47714940706024933 1101 90-91 0.39170721342499704 1101 92-93 0.24898549709060802 1101 94-95 0.07252720180403927 1101 96-97 0.15593248447314423 1101 98-99 0.07657810910277618 1101 100-101 0.35315728047305583 1102 1 0.24915129192065777 1102 2 0.3725832060212255 1102 3 0.22871467231542653 1102 4 0.25064278135278784 1102 5 0.18364203847618654 1102 6 0.2507741080913064 1102 7 0.24496630732468816 1102 8 0.22590879217077742 1102 9 0.22889971403903786 1102 10-11 0.3482641023335944 1102 12-13 0.3207987026630619 1102 14-15 0.3689335169431942 1102 16-17 0.41585818973459254 1102 18-19 0.4083993282613889 1102 20-21 0.49279375644949397 1102 22-23 0.5907418940637328 1102 24-25 0.7017847358104348 1102 26-27 0.5404325904749143 1102 28-29 0.5155370829645776 1102 30-31 0.43047116102215455 1102 32-33 0.446995480943734 1102 34-35 0.3888977797804145 1102 36-37 0.38256885262706675 1102 38-39 0.30603277531950823 1102 40-41 0.4556008275521535 1102 42-43 0.3701980949767574 1102 44-45 0.2779449784177004 1102 46-47 0.08805614529367745 1102 48-49 0.08295071168861767 1102 50-51 0.06236440554079792 1102 52-53 0.2109367438934271 1102 54-55 0.3777741982744658 1102 56-57 0.35949979489158324 1102 58-59 0.3712720159186489 1102 60-61 0.3181063104937536 1102 62-63 0.3225355992247927 1102 64-65 0.31045048095057126 1102 66-67 0.05443359333644793 1102 68-69 -0.13785140281806463 1102 70-71 0.1001225488232329 1102 72-73 0.048855069989681255 1102 74-75 0.1512388553261097 1102 76-77 0.3210720027301832 1102 78-79 0.39945752342878293 1102 80-81 0.3439623175428892 1102 82-83 0.20945949339249026 1102 84-85 0.19397632812489718 1102 86-87 0.42454734219698764 1102 88-89 0.1816719088559644 1102 90-91 0.15576779788301565 1102 92-93 0.20414257490852705 1102 94-95 0.14239132152558298 1102 96-97 0.12377838241171801 1102 98-99 0.2081648731971164 1102 100-101 0.19620502759316238 1103 1 0.34452655905412755 1103 2 0.4375963534198135 1103 3 0.3512119886703928 1103 4 0.2614610715015928 1103 5 0.2729507906058686 1103 6 0.15629995479362435 1103 7 0.18604429552649293 1103 8 0.40096615816086967 1103 9 0.4985346398325561 1103 10-11 0.4468400470258871 1103 12-13 0.33543564734550557 1103 14-15 0.4960988984147221 1103 16-17 0.5354256550934053 1103 18-19 0.4957359706029081 1103 20-21 0.36860992922699864 1103 22-23 0.415568811269587 1103 24-25 0.6314855463204694 1103 26-27 0.6101359615269999 1103 28-29 0.511383588317095 1103 30-31 0.6718022489570856 1103 32-33 0.6069899659300901 1103 34-35 0.5619854980419987 1103 36-37 0.5301077777902989 1103 38-39 0.6126847681105971 1103 40-41 0.7240071855750259 1103 42-43 0.70102505157557 1103 44-45 0.6730243059799577 1103 46-47 0.7784890738644066 1103 48-49 0.6194064290835612 1103 50-51 0.5444504594500472 1103 52-53 0.40256130933989454 1103 54-55 0.46598487179549863 1103 56-57 0.6282311447896518 1103 58-59 0.5751280937591261 1103 60-61 0.7072077572000097 1103 62-63 0.773141758313244 1103 64-65 0.8812297720743487 1103 66-67 0.7383666844031112 1103 68-69 0.7445926345658513 1103 70-71 0.8231112922864661 1103 72-73 0.790870610510261 1103 74-75 0.7073836638271089 1103 76-77 0.7195637449820822 1103 78-79 0.8232444666339909 1103 80-81 0.7581324557136782 1103 82-83 0.8063513892769123 1103 84-85 0.8142750252029245 1103 86-87 0.8463695617930149 1103 88-89 0.7659482110393192 1103 90-91 0.6307532717310167 1103 92-93 0.48228145476986484 1103 94-95 0.45654767216226944 1103 96-97 0.5093049184907379 1103 98-99 0.8260606000463682 1103 100-101 0.9056844496985157 1104 1 0.35560095678562575 1104 2 0.3963421084352987 1104 3 0.39320533195498797 1104 4 0.35678301402582946 1104 5 0.3358876677464764 1104 6 0.22683553537224554 1104 7 0.2780306342847112 1104 8 0.3076537900995788 1104 9 0.39178082719639207 1104 10-11 0.3557328970952014 1104 12-13 0.3830132888973239 1104 14-15 0.37855086910198565 1104 16-17 0.5050694870040999 1104 18-19 0.3823109504347073 1104 20-21 0.5443716110862411 1104 22-23 0.5922241740799024 1104 24-25 0.34649793708609167 1104 26-27 0.49661674255584387 1104 28-29 0.5237332929193101 1104 30-31 0.48786459407251215 1104 32-33 0.46126804079640493 1104 34-35 0.48107114166136 1104 36-37 0.4929475577032463 1104 38-39 0.4892565596752192 1104 40-41 0.5724002506108405 1104 42-43 0.6753256858475112 1104 44-45 0.5026102177036051 1104 46-47 0.42847772170097187 1104 48-49 0.2764032263242626 1104 50-51 0.5196290748725687 1104 52-53 0.6327614455658548 1104 54-55 0.5041522944679429 1104 56-57 0.7170062705088256 1104 58-59 0.7931017084328857 1104 60-61 0.7539315711404626 1104 62-63 0.7128593551327072 1104 64-65 0.5656372132967107 1104 66-67 0.3901088791869114 1104 68-69 0.4296896593328903 1104 70-71 0.48715609227100387 1104 72-73 0.47739994015293163 1104 74-75 0.48961293888278234 1104 76-77 0.3885406976749586 1104 78-79 0.4504152659712197 1104 80-81 0.6776262941458242 1104 82-83 0.6219850090712207 1104 84-85 0.5256027935608216 1104 86-87 0.5985530417383309 1104 88-89 0.6400358110953555 1104 90-91 0.6582170534989586 1104 92-93 0.523210834651902 1104 94-95 0.3632497962245118 1104 96-97 0.32917023835605974 1104 98-99 0.4269583870991056 1104 100-101 0.6338556886301596 1105 1 0.20377699988657483 1105 2 0.21076525865123585 1105 3 0.21326423571577635 1105 4 0.19827562105243857 1105 5 0.1788974682813418 1105 6 0.16968493992852984 1105 7 0.23341898230344071 1105 8 0.18221245788853224 1105 9 0.1724178376359191 1105 10-11 0.30325267899559805 1105 12-13 0.19332598807458368 1105 14-15 0.15172395241869907 1105 16-17 0.34839188940825494 1105 18-19 0.33792675584469833 1105 20-21 0.2958717756988918 1105 22-23 0.17828334705942694 1105 24-25 0.4141695518632815 1105 26-27 0.38528101220273214 1105 28-29 0.23749783032531013 1105 30-31 0.2218051291690628 1105 32-33 0.10848527598491131 1105 34-35 0.22616398157838802 1105 36-37 0.1914428520517646 1105 38-39 -0.04964797957963185 1105 40-41 0.2327366280619927 1105 42-43 0.2625095768366279 1105 44-45 0.5591591255026813 1105 46-47 0.5473280604138182 1105 48-49 0.6438746299431486 1105 50-51 0.5895241768756492 1105 52-53 0.6764782696281699 1105 54-55 0.7655965232130484 1105 56-57 0.8502354843090387 1105 58-59 0.7530379879273355 1105 60-61 0.6917850220867123 1105 62-63 0.6806605516964339 1105 64-65 0.9497284135743627 1105 66-67 0.7143289152232981 1105 68-69 0.8145937866506436 1105 70-71 0.8277660348755198 1105 72-73 0.6965500685183699 1105 74-75 0.7615980540062246 1105 76-77 0.637424899563058 1105 78-79 0.6306486915849483 1105 80-81 0.6300078257409147 1105 82-83 0.644427471676579 1105 84-85 0.710116959169266 1105 86-87 0.7623681792912613 1105 88-89 0.7660930216973867 1105 90-91 0.43220265840587757 1105 92-93 0.3631294851849063 1105 94-95 0.44175138315539897 1105 96-97 0.5923526308023668 1105 98-99 0.5625388048422408 1105 100-101 0.7090467771658204 1106 1 0.3797527259344342 1106 2 0.46667212887734877 1106 3 0.35544714726296434 1106 4 0.3308903791755071 1106 5 0.39878299926930794 1106 6 0.4121609437125642 1106 7 0.49157428327276875 1106 8 0.4008296919395562 1106 9 0.6262444354720387 1106 10-11 0.6126455647787239 1106 12-13 0.6469089743166947 1106 14-15 0.8796263902408654 1106 16-17 0.9359361328757672 1106 18-19 0.8393993263167019 1106 20-21 0.8036280086484524 1106 22-23 0.8108563973609577 1106 24-25 0.9083792739564842 1106 26-27 0.9613143708767282 1106 28-29 0.8498169937422446 1106 30-31 0.8042681276960195 1106 32-33 0.982915348082571 1106 34-35 0.9873544744700453 1106 36-37 0.9117435654450858 1106 38-39 1.1254528301208495 1106 40-41 1.1053832168361382 1106 42-43 1.183523493978356 1106 44-45 1.2943921869136474 1106 46-47 1.1309389719382494 1106 48-49 1.426108614613625 1106 50-51 1.4845403765727028 1106 52-53 1.3874624983842025 1106 54-55 1.3583807857144876 1106 56-57 1.4260460852997952 1106 58-59 1.615919259030072 1106 60-61 1.5483954274298597 1106 62-63 1.496252736409005 1106 64-65 1.5652728238731655 1106 66-67 1.527613899080471 1106 68-69 1.3755620906020773 1106 70-71 1.5319869612062398 1106 72-73 1.5147632106529372 1106 74-75 1.511316526210809 1106 76-77 1.5015109579653867 1106 78-79 1.5415593629102524 1106 80-81 1.5556645817444696 1106 82-83 1.563734211850118 1106 84-85 1.636659532428105 1106 86-87 1.8227331416878663 1106 88-89 1.5910182793853664 1106 90-91 1.6372947094638306 1106 92-93 1.7801726281346717 1106 94-95 1.981424052588686 1106 96-97 1.916267895675471 1106 98-99 1.9627324618409077 1106 100-101 2.088364721576877 1107 1 0.3204031348166403 1107 2 0.4590756074231024 1107 3 0.40981331890281325 1107 4 0.43907475107062055 1107 5 0.21325080887557846 1107 6 0.3564989465031232 1107 7 0.4133343049576155 1107 8 0.31013360252466526 1107 9 0.35258928107963783 1107 10-11 0.40046460941695017 1107 12-13 0.4515371874327059 1107 14-15 0.4226670074009533 1107 16-17 0.6229869450337162 1107 18-19 0.59318759290381 1107 20-21 0.5934273555679681 1107 22-23 0.609981983236942 1107 24-25 0.6137096119729222 1107 26-27 0.5130620778309876 1107 28-29 0.5227824062174662 1107 30-31 0.5189773567236884 1107 32-33 0.6214711802239208 1107 34-35 0.7619968548889418 1107 36-37 0.7965394071028058 1107 38-39 0.8881685451016281 1107 40-41 0.9288501401328162 1107 42-43 0.8829568018631875 1107 44-45 0.7319478250224307 1107 46-47 0.6492160602016241 1107 48-49 0.5497556274382944 1107 50-51 0.7024920946385009 1107 52-53 0.6715013204992388 1107 54-55 0.7462743232597973 1107 56-57 0.8837367466778474 1107 58-59 0.767992043670489 1107 60-61 0.7946320243130529 1107 62-63 0.7511613217165376 1107 64-65 0.8503539616645597 1107 66-67 0.8848527278257663 1107 68-69 0.9840628623446968 1107 70-71 0.7549245363451043 1107 72-73 0.7648029434535104 1107 74-75 0.8561484832779307 1107 76-77 0.9232105272910758 1107 78-79 0.9267744709298427 1107 80-81 0.9812969670483085 1107 82-83 0.8799956039509453 1107 84-85 0.869070577269131 1107 86-87 0.9880449929828679 1107 88-89 0.8749961616222208 1107 90-91 0.5770563711626835 1107 92-93 0.7529212896511872 1107 94-95 0.5976095867291562 1107 96-97 0.8556912191706694 1107 98-99 0.9322508222949253 1107 100-101 1.2144771181131482 1108 1 0.21981866956150853 1108 2 0.3311319299706881 1108 3 0.33735794595654767 1108 4 0.2847892914076411 1108 5 0.23839692518936317 1108 6 0.12342826358728587 1108 7 0.14061603404398682 1108 8 -0.029113697325072962 1108 9 -0.0736148634003726 1108 10-11 0.1106728236169019 1108 12-13 -0.11171923215442803 1108 14-15 0.4349504322039621 1108 16-17 0.5306706456072874 1108 18-19 0.5027848540119848 1108 20-21 0.3445827946100479 1108 22-23 0.44578462657450757 1108 24-25 0.4053047986131517 1108 26-27 0.5427247416161762 1108 28-29 0.40444366391935915 1108 30-31 0.17767910615864935 1108 32-33 0.29013309888398453 1108 34-35 0.23587786756597495 1108 36-37 0.48343461181418235 1108 38-39 0.5446534854049787 1108 40-41 0.3641628822675429 1108 42-43 0.45420660691766557 1108 44-45 0.27657240325932975 1108 46-47 0.2546351745176807 1108 48-49 0.09407943257694029 1108 50-51 0.4146064428768099 1108 52-53 0.3680361173474225 1108 54-55 0.41938099992747624 1108 56-57 0.48484849316697165 1108 58-59 0.38903279285543135 1108 60-61 0.12967840278215448 1108 62-63 0.28033878774651555 1108 64-65 0.5634708868970719 1108 66-67 0.8419374814765064 1108 68-69 0.6893473095286957 1108 70-71 0.6634325780028014 1108 72-73 0.5322446887791514 1108 74-75 0.5586665606075591 1108 76-77 0.6228805433075522 1108 78-79 0.6796838008370862 1108 80-81 0.689299511861023 1108 82-83 0.6914999892969824 1108 84-85 0.706132011721639 1108 86-87 0.8839329420073945 1108 88-89 1.0028767138564838 1108 90-91 0.9159905204204009 1108 92-93 0.8778683758653791 1108 94-95 0.7862562068069359 1108 96-97 0.88269024629966 1108 98-99 1.0280612882085443 1108 100-101 1.307308321279809 1109 1 0.09554201739842227 1109 2 0.15975526169250998 1109 3 -0.016749204748851554 1109 4 0.06367803379569636 1109 5 0.16973931227384753 1109 6 0.21252274197108534 1109 7 0.25191515455068014 1109 8 0.11856646206782528 1109 9 0.2463649194854014 1109 10-11 0.1113491691049191 1109 12-13 0.3200162654844618 1109 14-15 0.18015985513719812 1109 16-17 0.017605213454260138 1109 18-19 0.053660928961811294 1109 20-21 0.1340098298728023 1109 22-23 0.09179101401060308 1109 24-25 0.1326057161966716 1109 26-27 0.2139927551137859 1109 28-29 -0.026254119523116515 1109 30-31 0.20559972224346268 1109 32-33 0.23995656538657073 1109 34-35 0.42781836869647094 1109 36-37 0.5402915830560673 1109 38-39 0.5384442919897054 1109 40-41 0.22052248319928935 1109 42-43 0.32851255924629896 1109 44-45 0.408418264855122 1109 46-47 0.32353451379894693 1109 48-49 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-2.6722599034610326 2316 78-79 -2.746654121595423 2316 80-81 -2.6156818230518617 2316 82-83 -2.5326969183471206 2316 84-85 -2.142142968810237 2316 86-87 -2.103902887739899 2316 88-89 -2.5266301108537803 2316 90-91 -2.6686212258758637 2316 92-93 -2.898240828339585 2316 94-95 -2.982966033342411 2316 96-97 -2.7338812715215006 2316 98-99 -2.742647307309035 2316 100-101 -2.902905058202375 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2462.0 3 666.0 4 685.0 5 737.0 6 834.0 7 1020.0 8 1258.0 9 1656.0 10 2236.0 11 3206.0 12 4970.0 13 6611.0 14 7680.0 15 10503.0 16 14457.0 17 20377.0 18 16982.0 19 8954.0 20 7262.0 21 7100.0 22 7779.0 23 8407.0 24 9098.0 25 10331.0 26 11724.0 27 13895.0 28 16475.0 29 19693.0 30 23999.0 31 29966.0 32 37790.0 33 49338.0 34 67495.0 35 100850.0 36 141030.0 37 194824.0 38 177612.0 39 33516.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.65188329525636 19.513677414904734 15.035322563656583 18.79911672618232 2 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19.184075733227413 20-21 21.862124739843708 25.6036674753944 33.710289021113155 18.823918763648738 22-23 20.897833538894965 26.42208095492467 33.476107772094146 19.20397773408622 24-25 20.121002866838587 26.470460749572723 34.28023055888671 19.12830582470198 26-27 20.063148377715848 26.536066811449665 34.14122866703329 19.259556143801206 28-29 19.454216982029095 26.83330629960456 34.36723362921283 19.345243089153506 30-31 19.593086980349174 26.97584749080091 34.22784482147983 19.203220707370086 32-33 19.359595052988055 27.15730422427175 33.77539365317698 19.70770706956322 34-35 19.749937207034115 26.96928759923968 33.7567062654565 19.524068928269696 36-37 20.22799271899632 26.757502127354186 33.538845534047454 19.47565961960204 38-39 20.125972275101603 26.87932021945422 33.61304907242707 19.38165843301711 40-41 19.846246633673793 27.107210673060163 33.568879065808936 19.477663627457105 42-43 19.714806390872315 27.58585171237346 32.86274911911109 19.836592777643133 44-45 19.78940020159646 27.803628456696604 32.370398779796176 20.036572561910756 46-47 20.219842379974786 27.985661926758965 31.387250055214533 20.407245638051712 48-49 20.411433694562184 28.313734151969612 30.678264013927254 20.596568139540953 50-51 20.66429277260608 28.792915403118613 30.283362444051747 20.259429380223565 52-53 20.550040795709716 29.13568545878748 29.999221829409162 20.31505191609364 54-55 19.945402683171995 28.975396256942915 30.093718559126664 20.985482500758426 56-57 20.593042107769833 29.585977777581014 29.187208245120466 20.633771869528687 58-59 20.69333370616856 28.863644837164216 29.600274033890724 20.842747422776505 60-61 20.564068698978037 28.799632019692726 30.11603776178868 20.520261519540565 62-63 20.996012872526105 28.791301490686994 30.01717424283028 20.195511393956625 64-65 20.651502316309784 29.056384506492282 30.111281654136878 20.18083152306105 66-67 20.483277093159828 29.335371651223923 29.691409235817222 20.489942019799027 68-69 20.49370484151188 29.584075877444803 29.573170731707314 20.349048549336 70-71 20.369233263512555 29.772535835948506 29.23672038642445 20.62151051411448 72-73 20.367375005008093 29.682472981925212 29.061096860694906 20.889055152371785 74-75 20.26771216981506 30.10359519832645 29.056135225861762 20.572557405996733 76-77 20.470901374270042 29.84358894544858 29.14592276278045 20.539586917500927 78-79 20.26439396975931 29.7654347110766 29.11181374225969 20.8583575769044 80-81 20.091224414482586 30.3374063899026 28.888057420405787 20.683311775209027 82-83 20.13429981725792 30.150765713092603 28.745781575440883 20.9691528942086 84-85 20.233606484791483 29.778977560317195 28.653078383686605 21.334337571204724 86-87 20.46718700623904 29.81102034842803 28.638997875575267 21.082794769757662 88-89 20.57492553762326 29.491343530176316 28.627398145398487 21.306332786801942 90-91 19.986148112657045 29.69682450609539 29.115110488842987 21.20191689240458 92-93 20.039724159019695 30.138759535989006 28.787336374408355 21.03417993058294 94-95 19.93616184143299 29.98328292415501 28.60209630079921 21.47845893361279 96-97 20.027552897133518 29.83013708848396 28.349040660337117 21.793269354045407 98-99 20.01189196499565 29.833442537325617 28.36270959588305 21.79195590179569 100-101 20.157965815887795 29.73114007353147 28.785928665894417 21.324965444686317 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2695.0 1 1810.0 2 1128.0 3 1385.0 4 1299.0 5 1428.5 6 2454.0 7 4581.0 8 7204.0 9 8098.5 10 6524.5 11 5075.5 12 4866.0 13 4850.5 14 4738.0 15 4620.5 16 4535.5 17 4573.5 18 4599.0 19 4741.5 20 4939.5 21 5188.0 22 5819.5 23 6522.0 24 7278.0 25 8508.0 26 10080.0 27 11355.0 28 12222.5 29 13434.5 30 15417.5 31 17574.0 32 19993.0 33 22234.5 34 24241.5 35 25474.5 36 27324.5 37 30039.0 38 30306.0 39 29495.0 40 29741.5 41 30687.0 42 31209.5 43 32327.5 44 34241.5 45 35191.5 46 34759.0 47 35762.0 48 45736.0 49 45902.5 50 34999.5 51 30236.5 52 27237.0 53 26172.0 54 25129.5 55 23378.5 56 21722.0 57 19781.0 58 18287.5 59 17249.5 60 15647.0 61 13477.5 62 10986.0 63 7731.5 64 4978.0 65 3691.5 66 2732.5 67 1974.5 68 1403.5 69 987.0 70 689.0 71 477.5 72 377.5 73 361.0 74 289.0 75 200.0 76 156.0 77 90.5 78 50.0 79 34.5 80 20.5 81 11.5 82 7.5 83 9.5 84 11.0 85 7.0 86 5.5 87 6.5 88 5.5 89 4.5 90 2.5 91 0.5 92 1.0 93 1.5 94 1.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.061761171506022415 2 0.1004201250128087 3 0.07089027377991411 4 0.13237198297142963 5 0.10442575152074075 6 0.10349421047238447 7 0.11318223737528996 8 0.0951103410371778 9 0.11690840156871511 10-11 0.1073601058230631 12-13 0.0919431014727664 14-15 0.09590215092828065 16-17 0.11113284706890608 18-19 0.09208283263001985 20-21 0.07615348070312718 22-23 0.07629321186038063 24-25 0.06572022096153667 26-27 0.057755544998090345 28-29 0.07042450325573596 30-31 0.0519799904982813 32-33 0.052119721655534745 34-35 0.04839355746210957 36-37 0.052259452812788196 38-39 0.051747105236192234 40-41 0.06828195884451649 42-43 0.066139414433297 44-45 0.0516539511313566 46-47 0.03754110424875872 48-49 0.04527289495011598 50-51 0.02687495924507913 52-53 0.04289746527680742 54-55 0.05062925597816468 56-57 0.052585492179712895 58-59 0.05868708604644664 60-61 0.05649796458280934 62-63 0.061714594453604596 64-65 0.04163988486152642 66-67 0.066139414433297 68-69 0.05607877111104902 70-71 0.024778991886277472 72-73 0.021145981797687917 74-75 0.030042198809490543 76-77 0.04508658674044472 78-79 0.06492841107043382 80-81 0.03931103224063568 82-83 0.062087210872947114 84-85 0.06548733569944759 86-87 0.06790934242517396 88-89 0.09021975053330725 90-91 0.06632572264296827 92-93 0.07852891037643574 94-95 0.08700593391647803 96-97 0.0938527606218968 98-99 0.12459361521765457 100-101 0.12072771986697593 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1073490.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.50384177167918 #Duplication Level Percentage of deduplicated Percentage of total 1 74.96410193286259 37.11011040640459 2 13.60514248054337 13.470136412759395 3 4.654735699363263 6.912818986507963 4 2.1970584295861193 4.3505133144546075 5 1.2028768333842028 2.9773512215335036 6 0.735340049392051 2.1841294472089707 7 0.46710021261631024 1.6186278511812868 8 0.3511212318866784 1.3905479924796162 9 0.26173905813295706 1.166138002735402 >10 1.3792318742082286 12.526983941020683 >50 0.11336023101866967 3.8723549030054185 >100 0.0608249681835791 5.293871688189793 >500 0.004344640330934756 1.411368659347397 >1k 0.002455666274006601 2.3076761263748136 >5k 1.8889740569281546E-4 0.7488398743824259 >10k+ 3.777948113856309E-4 2.658531172414132 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16580 1.5444950581747385 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11850 1.1038761423022105 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8008 0.7459780715237216 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3495 0.32557359640052536 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3223 0.30023567988523414 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2630 0.2449952957177058 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2474 0.23046325536334758 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2162 0.20139917465463114 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1917 0.1785764189699019 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1804 0.16805000512347576 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1384 0.12892528109251133 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1171 0.10908345676252224 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1155 0.10759299108515216 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1152 0.10731352877064529 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1090 0.10153797427083626 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.315410483562958E-5 2 0.0 9.315410483562958E-5 0.0 0.0 9.315410483562958E-5 3 0.0 9.315410483562958E-5 0.0 0.0 9.315410483562958E-5 4 0.0 9.315410483562958E-5 0.0 0.0 9.315410483562958E-5 5 0.0 9.315410483562958E-5 0.0 0.0 1.8630820967125916E-4 6 0.0 9.315410483562958E-5 0.0 0.0 2.7946231450688876E-4 7 0.0 9.315410483562958E-5 0.0 0.0 2.7946231450688876E-4 8 0.0 9.315410483562958E-5 0.0 0.0 2.7946231450688876E-4 9 0.0 9.315410483562958E-5 0.0 0.0 2.7946231450688876E-4 10-11 0.0 9.315410483562958E-5 0.0 0.0 2.7946231450688876E-4 12-13 0.0 1.8630820967125916E-4 0.0 0.0 3.2603936692470354E-4 14-15 0.0 1.8630820967125916E-4 0.0 0.0 3.726164193425183E-4 16-17 0.0 1.8630820967125916E-4 0.0 0.0 3.726164193425183E-4 18-19 0.0 1.8630820967125916E-4 0.0 9.315410483562958E-5 3.726164193425183E-4 20-21 0.0 1.8630820967125916E-4 0.0 2.7946231450688876E-4 4.657705241781479E-4 22-23 0.0 1.8630820967125916E-4 0.0 6.986557862672219E-4 4.657705241781479E-4 24-25 0.0 1.8630820967125916E-4 0.0 0.0019096591491304066 4.657705241781479E-4 26-27 0.0 1.8630820967125916E-4 0.0 0.0031672395644114057 4.657705241781479E-4 28-29 0.0 1.8630820967125916E-4 0.0 0.005496092185302145 4.657705241781479E-4 30-31 0.0 1.8630820967125916E-4 0.0 0.010153797427083625 4.657705241781479E-4 32-33 0.0 1.8630820967125916E-4 0.0 0.014858079721282919 4.657705241781479E-4 34-35 0.0 1.8630820967125916E-4 0.0 0.019282899700975322 4.657705241781479E-4 36-37 0.0 1.8630820967125916E-4 0.0 0.024685837781441837 4.657705241781479E-4 38-39 0.0 1.8630820967125916E-4 0.0 0.03372178595049791 4.657705241781479E-4 40-41 0.0 1.8630820967125916E-4 0.0 0.05202656755069912 4.657705241781479E-4 42-43 0.0 1.8630820967125916E-4 0.0 0.06665176200989296 4.657705241781479E-4 44-45 0.0 1.8630820967125916E-4 0.0 0.08360580908997756 5.123475765959627E-4 46-47 0.0 2.7946231450688876E-4 0.0 0.11835229019366739 5.589246290137775E-4 48-49 0.0 2.7946231450688876E-4 0.0 0.14438886249522587 5.589246290137775E-4 50-51 0.0 2.7946231450688876E-4 0.0 0.17340636615152447 5.589246290137775E-4 52-53 0.0 2.7946231450688876E-4 0.0 0.20479929948113162 6.520787338494071E-4 54-55 0.0 2.7946231450688876E-4 0.0 0.24830226643937064 7.452328386850366E-4 56-57 0.0 2.7946231450688876E-4 0.0 0.35379929016572115 7.452328386850366E-4 58-59 0.0 3.2603936692470354E-4 0.0 0.43721879104602746 7.452328386850366E-4 60-61 0.0 3.726164193425183E-4 0.0 0.500703313491509 7.452328386850366E-4 62-63 0.0 3.726164193425183E-4 0.0 0.5760184072511155 7.452328386850366E-4 64-65 0.0 3.726164193425183E-4 0.0 0.6443469431480497 7.452328386850366E-4 66-67 0.0 3.726164193425183E-4 0.0 0.761488229978854 7.452328386850366E-4 68-69 0.0 3.726164193425183E-4 0.0 0.9102553354013545 7.452328386850366E-4 70-71 0.0 3.726164193425183E-4 0.0 0.9879924358866874 7.452328386850366E-4 72-73 0.0 3.726164193425183E-4 0.0 1.0756504485370149 7.452328386850366E-4 74-75 0.0 3.726164193425183E-4 0.0 1.2370865122171608 7.452328386850366E-4 76-77 0.0 3.726164193425183E-4 0.0 1.409095566796151 7.452328386850366E-4 78-79 0.0 4.657705241781479E-4 0.0 1.5730002142544413 7.452328386850366E-4 80-81 0.0 4.657705241781479E-4 0.0 1.720928932733421 7.452328386850366E-4 82-83 0.0 4.657705241781479E-4 0.0 1.946268712330809 7.452328386850366E-4 84-85 0.0 4.657705241781479E-4 0.0 2.321540023661143 7.452328386850366E-4 86-87 0.0 4.657705241781479E-4 0.0 2.6503274366784972 8.849639959384811E-4 88-89 0.0 4.657705241781479E-4 0.0 3.072408685688735 0.0010246951531919255 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATC 40 5.36574E-4 47.539932 1 CGTCACT 170 3.6379788E-12 33.5576 1 GGTATCA 11430 0.0 30.695074 1 GTATCAA 16970 0.0 30.619413 1 AATCGAC 35 0.0083902795 27.144768 20-21 GTACATG 12380 0.0 26.803614 1 TACATGG 12475 0.0 26.448298 2 GAGTACT 9905 0.0 25.379757 12-13 TATCAAC 20420 0.0 25.35426 2 ATCAACG 20660 0.0 25.081572 3 ATGGGAT 3380 0.0 25.034653 5 ACATGGG 12495 0.0 24.996986 3 TCAACGC 20800 0.0 24.936773 4 CAACGCA 21100 0.0 24.602455 5 AACGCAG 21400 0.0 24.25756 6 CATGGGG 2340 0.0 23.771074 4 CATGGGA 9870 0.0 23.457981 4 GTACTTT 10605 0.0 23.435118 14-15 AGTACTT 10000 0.0 22.92892 12-13 ACGCAGA 23245 0.0 22.229939 7 >>END_MODULE