FastQCFastQC Report
Thu 26 May 2016
SRR936823_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936823_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073490
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206781.9262405797911486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156681.4595385145646445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112241.0455616726751065No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40030.3728958816570252No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34570.32203374041677146No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33730.3142087956105786No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA32030.29837259778852154No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29440.2742456846360935No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25860.2408965151049381No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18830.1754091794054905No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16810.15659205022869332No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT16500.1537042729787888No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16300.15184119088207623No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14350.13367614043912845No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT12400.11551108999618069No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG12030.1120643881172624No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA11840.11029446012538542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC200.002072534871.263041
GGTATCA118850.032.818371
GTATCAA177350.031.5563771
TATCAAC221400.025.2493692
ATCAACG223150.025.0939243
TCAACGC224900.024.9197794
CAACGCA227650.024.6813415
GTACATG109450.024.5682221
TAAGGTG5050.024.4530815
GAGTACT133600.024.42316412-13
AACGCAG232200.024.2181596
AGTACTT134800.023.92387612-13
TACATGG111250.023.7797262
GTAAGGT4350.022.928824
GTACTTT144050.022.60405314-15
TAATACC2755.2750693E-1122.4523774
AGGTAAG3600.022.4283892
ACATGGG110850.022.1945173
ACGCAGA253000.022.1707847
CGCAGAG254200.022.0100678
Produced by FastQC (version 0.11.5)