##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936823_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1073490 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.094878387316136 31.0 31.0 34.0 31.0 34.0 2 32.041911894847644 33.0 31.0 34.0 30.0 34.0 3 32.18197188609116 33.0 31.0 34.0 30.0 34.0 4 35.97047760109549 37.0 35.0 37.0 35.0 37.0 5 35.923756159815184 37.0 35.0 37.0 35.0 37.0 6 36.00505547326943 37.0 35.0 37.0 35.0 37.0 7 35.942693457787215 37.0 35.0 37.0 35.0 37.0 8 35.93418383031048 37.0 35.0 37.0 35.0 37.0 9 37.56859123047257 39.0 37.0 39.0 35.0 39.0 10-11 37.51720789201576 39.0 37.0 39.0 35.0 39.0 12-13 37.403995845326925 39.0 37.0 39.0 34.0 39.0 14-15 38.4416953115539 40.0 38.0 41.0 34.0 41.0 16-17 38.4049008374554 40.0 38.0 41.0 34.0 41.0 18-19 38.614371349523516 40.0 38.0 41.0 34.0 41.0 20-21 38.743775908485404 40.0 38.0 41.0 35.0 41.0 22-23 38.66452179340283 40.0 38.0 41.0 34.0 41.0 24-25 38.54636792145246 40.0 38.0 41.0 34.0 41.0 26-27 38.33982337981723 40.0 38.0 41.0 34.0 41.0 28-29 38.12285070191618 40.0 37.0 41.0 34.0 41.0 30-31 37.78662772825085 39.5 37.0 41.0 33.0 41.0 32-33 37.524850720547 40.0 35.5 41.0 33.0 41.0 34-35 37.335723667663416 40.0 35.0 41.0 33.0 41.0 36-37 37.076072902402444 39.0 35.0 41.0 33.0 41.0 38-39 36.82958667523684 39.0 35.0 41.0 32.0 41.0 40-41 36.52514695060037 39.0 35.0 41.0 31.0 41.0 42-43 36.14846947805755 39.0 35.0 41.0 30.5 41.0 44-45 35.539457284185225 39.0 35.0 41.0 28.0 41.0 46-47 34.8518877679345 39.0 35.0 41.0 24.0 41.0 48-49 34.20474107816561 39.0 34.5 40.0 19.0 41.0 50-51 33.84154393613355 38.0 34.0 40.0 13.5 41.0 52-53 33.55396929640705 38.0 34.0 40.0 8.5 41.0 54-55 33.31300151841191 38.0 33.0 40.0 7.0 41.0 56-57 33.05763863659652 38.0 33.0 40.0 2.0 41.0 58-59 32.739656633969574 37.0 33.0 40.0 2.0 41.0 60-61 32.41419901442957 37.0 32.5 40.0 2.0 41.0 62-63 32.045274758032214 36.0 32.0 39.5 2.0 41.0 64-65 31.63811120737035 36.0 31.0 39.0 2.0 40.5 66-67 31.272691874167435 35.0 31.0 39.0 2.0 40.0 68-69 30.848666498989278 35.0 31.0 38.0 2.0 40.0 70-71 30.37675106428565 35.0 30.0 37.5 2.0 40.0 72-73 30.06489580713374 35.0 30.0 37.0 2.0 39.0 74-75 29.64104183550848 34.5 29.0 36.5 2.0 39.0 76-77 28.27352746648781 33.0 28.0 35.0 2.0 37.5 78-79 28.930981192186234 34.0 29.0 36.0 2.0 38.0 80-81 28.76729359379221 34.0 29.0 35.0 2.0 37.0 82-83 28.4378713355504 34.0 29.0 35.0 2.0 37.0 84-85 28.075215884637956 34.0 28.5 35.0 2.0 36.0 86-87 27.705364744897484 34.0 27.0 35.0 2.0 36.0 88-89 27.3301637649163 34.0 26.5 35.0 2.0 35.5 90-91 26.911225069632692 33.0 26.0 35.0 2.0 35.0 92-93 26.48287454936702 33.0 25.0 35.0 2.0 35.0 94-95 26.04819094728409 33.0 24.0 35.0 2.0 35.0 96-97 25.47448928262024 33.0 21.5 35.0 2.0 35.0 98-99 24.80941648268731 32.0 10.0 34.5 2.0 35.0 100-101 22.085490316630803 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3610749573838845 1101 2 0.18960446179232093 1101 3 0.246570394682891 1101 4 0.2101459379319195 1101 5 0.20583912709055596 1101 6 0.241462630581033 1101 7 0.22988530650538763 1101 8 0.22586341025912304 1101 9 0.2888363523813524 1101 10-11 0.36919785443539155 1101 12-13 0.38884585973239894 1101 14-15 0.470101105316175 1101 16-17 0.46184170573352645 1101 18-19 0.3680283581810144 1101 20-21 0.40541938248937015 1101 22-23 0.4068135110818929 1101 24-25 0.2817994739459522 1101 26-27 0.3140912975104868 1101 28-29 0.2986622283874354 1101 30-31 0.4278247747933932 1101 32-33 0.5168957078147756 1101 34-35 0.4085064827879705 1101 36-37 0.41188987708014224 1101 38-39 0.3314784251743035 1101 40-41 0.5596210535226263 1101 42-43 0.8162957639033053 1101 44-45 0.7598125514189249 1101 46-47 0.6081595570348668 1101 48-49 0.7724957713472875 1101 50-51 1.0979124027375207 1101 52-53 0.8571340428408476 1101 54-55 0.737455928134743 1101 56-57 0.8203409139525704 1101 58-59 0.9515718176740577 1101 60-61 0.9862718224966187 1101 62-63 1.0051356433916538 1101 64-65 1.0696057502461294 1101 66-67 1.0120270523242532 1101 68-69 0.9312075774343747 1101 70-71 0.8523314304239271 1101 72-73 0.9445992169298343 1101 74-75 0.7371843225830403 1101 76-77 0.7413675924047318 1101 78-79 0.829015804161088 1101 80-81 1.008377795214077 1101 82-83 1.201180579876194 1101 84-85 0.994371782379762 1101 86-87 0.9932518070592451 1101 88-89 0.8399697158741333 1101 90-91 0.8937458536505432 1101 92-93 0.9549780498986884 1101 94-95 0.8769154998291881 1101 96-97 0.901782513941022 1101 98-99 0.9653758465108027 1101 100-101 1.357759476372415 1102 1 0.20344087282055767 1102 2 0.047775370667203276 1102 3 0.20551197588432046 1102 4 0.20771564678303633 1102 5 0.14208448928494732 1102 6 0.1546688326840453 1102 7 0.16696506866089322 1102 8 0.22272563435357284 1102 9 0.3352225094429713 1102 10-11 0.3356773886835924 1102 12-13 0.3637585042672171 1102 14-15 0.5248434134446924 1102 16-17 0.46362526940446 1102 18-19 0.4984229294506477 1102 20-21 0.3970618812607256 1102 22-23 0.4206864230567433 1102 24-25 0.43092888964996234 1102 26-27 0.43956317904189746 1102 28-29 0.34498897833207565 1102 30-31 0.3550861607074296 1102 32-33 0.39028293960515015 1102 34-35 0.3108695358817286 1102 36-37 0.19606787160388706 1102 38-39 0.26381371894249384 1102 40-41 0.3167931127353185 1102 42-43 0.4913725881068487 1102 44-45 0.6676208068479283 1102 46-47 0.6007890240450138 1102 48-49 0.39384695921959434 1102 50-51 0.5495739117863039 1102 52-53 0.675122787892505 1102 54-55 0.4918318023644801 1102 56-57 0.537480677296216 1102 58-59 0.4022801125277766 1102 60-61 0.423088303110994 1102 62-63 0.446996328301708 1102 64-65 0.5921872055044695 1102 66-67 0.7747590776208817 1102 68-69 0.5227755472771598 1102 70-71 0.439141430261909 1102 72-73 0.47721912479479656 1102 74-75 0.32562396765251833 1102 76-77 0.3886202832770991 1102 78-79 0.40624775654898215 1102 80-81 0.22718635847995827 1102 82-83 0.2695500705411753 1102 84-85 0.1987687191327865 1102 86-87 0.2155923934614954 1102 88-89 0.10744620955807349 1102 90-91 0.5379134249255841 1102 92-93 0.3826498849845308 1102 94-95 0.4211265463125571 1102 96-97 0.8011158040691804 1102 98-99 0.7925011361204994 1102 100-101 0.9023863726745915 1103 1 0.06707231233760069 1103 2 -0.1398188031148173 1103 3 -0.09599185015639478 1103 4 0.026286470391234218 1103 5 0.1296215432828376 1103 6 0.06717708058391025 1103 7 0.10438936032632995 1103 8 0.20950437397598876 1103 9 0.18113696764211795 1103 10-11 0.269447983812654 1103 12-13 0.3142095535970455 1103 14-15 0.23620138707110527 1103 16-17 0.21429987375147874 1103 18-19 0.343734183032943 1103 20-21 0.311347736235561 1103 22-23 0.3555944897490164 1103 24-25 0.39365330638802476 1103 26-27 0.33635856218762683 1103 28-29 0.2516695310152244 1103 30-31 0.285739788306131 1103 32-33 0.3937716781442546 1103 34-35 0.27273223173857275 1103 36-37 0.3732204503651815 1103 38-39 0.5352188656320109 1103 40-41 0.4540923317699921 1103 42-43 0.4581938721201979 1103 44-45 0.5384193020370631 1103 46-47 0.23165734324612686 1103 48-49 -0.00294842392612793 1103 50-51 -0.006423173135061688 1103 52-53 -0.017873144784935846 1103 54-55 0.14027922754248578 1103 56-57 0.11317916362193614 1103 58-59 0.2514395653596395 1103 60-61 0.11139401213493727 1103 62-63 0.19115139866737252 1103 64-65 -0.058448990711834625 1103 66-67 0.10059297722793659 1103 68-69 0.2474631119062245 1103 70-71 -0.03109965462006059 1103 72-73 0.150122188581836 1103 74-75 0.1030488502122715 1103 76-77 -0.03739728598063863 1103 78-79 0.04516208672102806 1103 80-81 0.23117981924950115 1103 82-83 0.34394120318710364 1103 84-85 0.295648304718906 1103 86-87 0.07767765951166083 1103 88-89 0.23432599210896754 1103 90-91 0.42808832964387733 1103 92-93 0.47343587291058853 1103 94-95 0.40615476151626595 1103 96-97 0.49128283019655683 1103 98-99 0.26131915051862364 1103 100-101 0.4785403400375259 1104 1 0.18047800967443095 1104 2 0.0631047412721415 1104 3 0.045318431039113705 1104 4 0.17625338004250324 1104 5 0.07547172370977506 1104 6 0.09450922556311525 1104 7 0.1719733784125026 1104 8 0.14467385936067956 1104 9 0.11825564364171015 1104 10-11 0.09921504708043472 1104 12-13 0.1267321975400577 1104 14-15 0.34342257069113913 1104 16-17 0.37218431355844217 1104 18-19 0.3806552830817367 1104 20-21 0.1328192664588812 1104 22-23 0.0401359831140482 1104 24-25 0.17003138656811245 1104 26-27 0.057707633967112315 1104 28-29 0.06988878718283331 1104 30-31 0.02509170054183585 1104 32-33 0.09939921124668416 1104 34-35 -0.03853805119598519 1104 36-37 0.07899894308771849 1104 38-39 -0.07696345494343149 1104 40-41 0.17273620715705107 1104 42-43 0.06231615665856083 1104 44-45 0.17144129624987414 1104 46-47 0.30282453887530636 1104 48-49 0.250860589208564 1104 50-51 0.29710469280313134 1104 52-53 0.37891757445915886 1104 54-55 0.44477565040332223 1104 56-57 0.4188820557667299 1104 58-59 0.2839647212707348 1104 60-61 0.43298389470042764 1104 62-63 0.48434154982022193 1104 64-65 0.36201581999687704 1104 66-67 0.4493374762593838 1104 68-69 0.4099101552714899 1104 70-71 0.35657793427703766 1104 72-73 0.3600466363111643 1104 74-75 0.14389369270703867 1104 76-77 0.010462951980027668 1104 78-79 0.17499460608009088 1104 80-81 0.41172148652000473 1104 82-83 0.465557896181128 1104 84-85 0.5830198682741106 1104 86-87 0.4034738809319869 1104 88-89 0.577222919295707 1104 90-91 0.6210159318254256 1104 92-93 0.4915051355996489 1104 94-95 0.37151134021573995 1104 96-97 0.5722744779026918 1104 98-99 0.5540636688728995 1104 100-101 0.7136339833090766 1105 1 0.11406937132113626 1105 2 -0.03993305152349791 1105 3 -0.03875403745337991 1105 4 0.017316210180084113 1105 5 0.008942261287685938 1105 6 0.04174640888647474 1105 7 0.06977081240120242 1105 8 0.08669533523073625 1105 9 0.1776135083155097 1105 10-11 0.15672608748108274 1105 12-13 0.10626224867539946 1105 14-15 0.25723973549354895 1105 16-17 0.1433378509465939 1105 18-19 0.15178165532690713 1105 20-21 0.06535443112829853 1105 22-23 0.10977159718412821 1105 24-25 0.176813346967009 1105 26-27 0.13649425217440836 1105 28-29 0.1597275640864595 1105 30-31 0.41591336908167875 1105 32-33 0.2939235293205016 1105 34-35 0.29944619788648197 1105 36-37 0.30576828588587546 1105 38-39 0.43587795414092056 1105 40-41 0.2608733108500516 1105 42-43 0.18411289976063472 1105 44-45 0.09488505791669866 1105 46-47 0.2423549305885544 1105 48-49 0.16564718471067152 1105 50-51 -0.020424203610900804 1105 52-53 0.040593558364413695 1105 54-55 0.09405662555898431 1105 56-57 0.16774557971016435 1105 58-59 0.18163362764261848 1105 60-61 0.3483778541457099 1105 62-63 0.2442329054662764 1105 64-65 0.16977580663325043 1105 66-67 0.2699814442191091 1105 68-69 0.010876099102080872 1105 70-71 -0.15274700786526196 1105 72-73 -0.09783766126311022 1105 74-75 0.03142837894558426 1105 76-77 0.15613055766865358 1105 78-79 0.2265682382137797 1105 80-81 0.2872167435671322 1105 82-83 0.23032884131727016 1105 84-85 0.37664546610998073 1105 86-87 0.24370107883532555 1105 88-89 0.16883797219069763 1105 90-91 0.2904501994774975 1105 92-93 0.6805287149437653 1105 94-95 0.817985872286016 1105 96-97 0.845457697466788 1105 98-99 0.8185929658714315 1105 100-101 0.6925432906546938 1106 1 0.39509193335678106 1106 2 0.22880940078265866 1106 3 0.18984653242712568 1106 4 0.2471481087011611 1106 5 0.30208378919684975 1106 6 0.21887857907713482 1106 7 0.23587221169861294 1106 8 0.35633355779504683 1106 9 0.49358438108959746 1106 10-11 0.3851906329890582 1106 12-13 0.40860935633276796 1106 14-15 0.5222924674506473 1106 16-17 0.5751702082537093 1106 18-19 0.5793934654653228 1106 20-21 0.5907966762224248 1106 22-23 0.5613530819446524 1106 24-25 0.643131911307961 1106 26-27 0.6315608804193218 1106 28-29 0.7694115515058613 1106 30-31 0.6159720444033496 1106 32-33 0.4830144694337619 1106 34-35 0.4697871024532958 1106 36-37 0.26758430131017974 1106 38-39 0.33299992487340546 1106 40-41 0.17808281113347135 1106 42-43 0.21821425735023325 1106 44-45 0.38405012420370355 1106 46-47 0.13252780950885068 1106 48-49 0.1557387333789606 1106 50-51 0.029017575113940097 1106 52-53 -0.057592814931958 1106 54-55 0.08509228764221177 1106 56-57 -0.3005981837382379 1106 58-59 -0.370810640280844 1106 60-61 -0.28564928247963906 1106 62-63 -0.20407830692322193 1106 64-65 -0.06446479820599649 1106 66-67 -0.043509235030679605 1106 68-69 -0.17368764656362146 1106 70-71 -0.22818934774988264 1106 72-73 -0.006856788090907173 1106 74-75 -0.07645364100212859 1106 76-77 -0.043435680987290226 1106 78-79 0.05197692746965643 1106 80-81 -0.08892334921381817 1106 82-83 0.09245889767970183 1106 84-85 0.016067604242497424 1106 86-87 -0.15235592059024583 1106 88-89 0.195797813245985 1106 90-91 0.5046388212046153 1106 92-93 0.42806226604247755 1106 94-95 0.5838177821819563 1106 96-97 0.9270524149816062 1106 98-99 1.2181605315944282 1106 100-101 0.9798438277376853 1107 1 0.23568198738364288 1107 2 0.14684282703711915 1107 3 0.1318190929062979 1107 4 0.18975346887534528 1107 5 0.28451165344425533 1107 6 0.22993551057388117 1107 7 0.18760295299079388 1107 8 0.2231745612652105 1107 9 0.3322126585581202 1107 10-11 0.2568307737455484 1107 12-13 0.28097102524689177 1107 14-15 0.3294645548413584 1107 16-17 0.23288066290420062 1107 18-19 0.3163715885365761 1107 20-21 0.3531384641106925 1107 22-23 0.3119632120049829 1107 24-25 0.4082159937142791 1107 26-27 0.43200666781617514 1107 28-29 0.37032018946545975 1107 30-31 0.29866931166419874 1107 32-33 0.2944523482969785 1107 34-35 0.37818179005598296 1107 36-37 0.3454011131112651 1107 38-39 0.3702298027693942 1107 40-41 0.553631391916845 1107 42-43 0.4760006674648807 1107 44-45 0.5619316785313586 1107 46-47 0.808788850595981 1107 48-49 0.5459094722920455 1107 50-51 0.5791852329067808 1107 52-53 0.668777869183991 1107 54-55 0.5263379572544622 1107 56-57 0.6452881794770917 1107 58-59 0.6251366140607573 1107 60-61 0.5924651109345689 1107 62-63 0.4602741069297238 1107 64-65 0.2962895302316717 1107 66-67 0.34219631712849363 1107 68-69 0.38657214257464645 1107 70-71 0.4962444232780321 1107 72-73 0.38484476056202865 1107 74-75 0.4501029828701206 1107 76-77 0.39773783580919897 1107 78-79 0.47266343226050367 1107 80-81 0.6020339528948995 1107 82-83 0.5447142094432458 1107 84-85 0.5369343935689486 1107 86-87 0.5582932499936355 1107 88-89 0.7373046955520657 1107 90-91 0.7795049728561843 1107 92-93 0.7740886447219104 1107 94-95 0.5723498721467664 1107 96-97 0.7025572165564888 1107 98-99 0.7640682481430687 1107 100-101 1.1723967796166335 1108 1 0.13365678185535756 1108 2 -0.021206405682043794 1108 3 0.1267839284465424 1108 4 0.11029243446343173 1108 5 0.15393899631816055 1108 6 0.1737466399211698 1108 7 0.16619731528993498 1108 8 0.10802673685728337 1108 9 0.1831727807487411 1108 10-11 0.2671337995476719 1108 12-13 0.18882305973554736 1108 14-15 0.2523218250098651 1108 16-17 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-1.5323537018190194 2316 56-57 -1.614470863637706 2316 58-59 -1.6224261467049956 2316 60-61 -1.2744262681618466 2316 62-63 -1.308084449460381 2316 64-65 -1.210763562647184 2316 66-67 -1.3175424775085567 2316 68-69 -1.1289000542101029 2316 70-71 -0.9422827968419547 2316 72-73 -1.0217469562881298 2316 74-75 -1.0577143758605914 2316 76-77 -1.3585732569311126 2316 78-79 -1.0989400568719248 2316 80-81 -1.4663868713703891 2316 82-83 -1.3947397972052578 2316 84-85 -1.2192431048914791 2316 86-87 -1.1664956824624646 2316 88-89 -1.3341218097053051 2316 90-91 -1.3779269416373872 2316 92-93 -1.7441372689145247 2316 94-95 -1.537215706659012 2316 96-97 -1.3107702401509727 2316 98-99 -1.4047541963049284 2316 100-101 -1.2403730066096799 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 11.0 5 45.0 6 106.0 7 229.0 8 502.0 9 1041.0 10 1798.0 11 3019.0 12 4935.0 13 6707.0 14 8595.0 15 12465.0 16 17382.0 17 25289.0 18 21189.0 19 10327.0 20 7602.0 21 7114.0 22 7116.0 23 7641.0 24 8480.0 25 9923.0 26 11377.0 27 14002.0 28 17076.0 29 20720.0 30 25606.0 31 31086.0 32 39348.0 33 51115.0 34 70386.0 35 102356.0 36 141712.0 37 209543.0 38 163673.0 39 13967.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.753159438438004 19.75920811857351 16.031890669081665 18.455741773906823 2 15.470298297929027 22.642546464366994 40.33957578179657 21.54757945590741 3 19.04166592918087 29.142861401421747 31.82948334694965 19.985989322447733 4 11.873828001643243 18.36752564306787 38.12584595807868 31.632800397210215 5 11.476735390451207 39.987479925028595 32.63035223889496 15.90543244562524 6 27.11637993672938 38.594799736186104 17.80613404577991 16.482686281304613 7 24.896109787804065 37.78585528123716 20.43793648560907 16.880098445349695 8 27.39252345154589 32.24995109409496 19.743919365806853 20.61360608855229 9 28.68745866286598 15.861349430362647 20.822457591593775 34.6287343151776 10-11 24.113661155354176 28.450128506207328 25.845490731623705 21.590719606814787 12-13 27.32549934349308 26.91119433955497 27.422473263470504 18.340833053481443 14-15 23.84451648361885 25.826556372206543 29.63804972566116 20.69087741851345 16-17 19.08303756905048 29.816812452840736 29.58071337413483 21.519436603973954 18-19 18.829378941583062 25.895304101575235 36.333361279564784 18.94195567727692 20-21 20.99991755696604 24.303461163508757 36.4088466499017 18.287774629623506 22-23 20.111365732330995 24.99809034085087 36.271693262163595 18.618850664654538 24-25 19.5164121847622 25.039836304346103 36.966460183957324 18.477291326934374 26-27 19.50321130124656 25.081545951953217 36.887182695071004 18.528060051729224 28-29 18.893545152168517 25.179686892300584 37.15297524976373 18.773792705767168 30-31 19.00715530172454 25.374302404665855 37.017832353516624 18.600709940092983 32-33 18.91182406453004 25.48937595279671 36.55949387252447 19.039306110148775 34-35 19.279959234890313 25.230724372523795 36.55422104265897 18.935095349926918 36-37 19.70989745194923 25.0054031494772 36.33105655793294 18.953642840640626 38-39 19.64263423340842 25.13693959566488 36.39225648506799 18.828169685858704 40-41 19.361497973822722 25.524570310680517 36.13829242163119 18.975639293865573 42-43 19.300862161318722 25.87522648619191 35.49230762064902 19.33160373184035 44-45 19.496519052638295 26.1159781701959 34.738815998151814 19.64868677901399 46-47 20.013675041698164 26.403636368717503 33.56724361742114 20.015444972163184 48-49 20.259928261923626 26.802010454699793 32.668118941190336 20.269942342186244 50-51 20.48574366923524 27.308269864218325 32.16030360842413 20.04568285812231 52-53 20.350419754742504 27.724260445428158 31.848960953307177 20.076358846522165 54-55 19.846449297200213 27.58444333794616 31.832682553974006 20.736424810879623 56-57 20.40322798506177 28.030753982116224 31.03600695676799 20.53001107605402 58-59 20.563830049851905 27.385385561624602 31.39538295794191 20.65540143058158 60-61 20.514699135476487 27.201726345540187 31.988581079413102 20.294993439570224 62-63 20.88966827823268 27.235512207845442 31.91869509729946 19.956124416622416 64-65 20.526060350874577 27.50983009606992 31.85621836832981 20.107891184725695 66-67 20.36792162382585 27.868460753458695 31.520895174102776 20.242722448612678 68-69 20.544019972240076 27.986706909239956 31.292513204594357 20.176759913925608 70-71 20.424085049895783 28.189056696049093 30.952852268889952 20.434005985165175 72-73 20.463590934051986 28.015923770097423 30.785908944614736 20.73457635123585 74-75 20.362202971346168 28.412187763888973 30.675886468996115 20.54972279576874 76-77 20.371258790311536 28.163976513833937 30.867652305625704 20.597112390228816 78-79 20.20590869648449 28.127068320904197 30.80605187494382 20.8609711076675 80-81 20.112358713180186 28.681934757233073 30.562110305495338 20.6435962240914 82-83 20.00054961664239 28.530367018157378 30.373958231929837 21.095125133270393 84-85 20.06643023428046 28.17313595234835 30.263950954719192 21.496482858652 86-87 20.201215678054915 28.16893732970027 30.178439181163974 21.451407811080834 88-89 20.30606137529664 27.817350619831345 30.238453996474075 21.63813400839794 90-91 19.773495266596026 27.931065801884046 30.693929831507123 21.60150910001281 92-93 19.710430465118446 28.26113890208572 30.454685185702708 21.573745447093128 94-95 19.546934851623867 28.042139269372363 30.289952006915772 22.120973872087998 96-97 19.705415069635897 27.853194736913196 30.044020525833588 22.39736966761732 98-99 19.60380636536822 27.868398131285137 30.004704391852556 22.523091111494086 100-101 19.4778210171102 27.903435780275878 30.333703623487107 22.285039579126813 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3876.0 1 2638.0 2 1614.5 3 1809.0 4 1437.0 5 1333.5 6 2557.5 7 5572.5 8 8876.0 9 9713.5 10 7508.5 11 5270.0 12 4773.0 13 4749.5 14 4723.5 15 4697.5 16 4702.0 17 4763.0 18 4868.0 19 4994.5 20 5257.0 21 5597.0 22 6167.0 23 6875.5 24 7545.5 25 8643.5 26 10059.5 27 11349.5 28 12427.0 29 13569.0 30 15233.5 31 17238.5 32 19727.5 33 21752.0 34 23684.0 35 25011.0 36 26807.5 37 29677.5 38 29969.0 39 29158.0 40 29368.0 41 30282.0 42 30872.0 43 31885.0 44 33916.5 45 35056.0 46 34689.0 47 35913.0 48 46265.0 49 45728.0 50 34298.0 51 30018.0 52 27013.5 53 25972.0 54 24725.0 55 22721.0 56 21234.0 57 19661.5 58 18319.0 59 17190.5 60 15230.0 61 12679.5 62 10370.0 63 7510.0 64 4859.0 65 3554.0 66 2658.0 67 1951.0 68 1415.0 69 948.5 70 683.0 71 517.5 72 385.0 73 352.0 74 296.5 75 249.0 76 189.0 77 82.0 78 40.0 79 30.5 80 20.5 81 17.0 82 14.0 83 8.0 84 5.0 85 5.0 86 4.0 87 1.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018444512757454658 2 0.003074085459575776 3 0.0021425444112194805 4 2.7946231450688876E-4 5 0.0013041574676988142 6 5.589246290137775E-4 7 9.315410483562958E-5 8 0.0 9 0.0 10-11 9.315410483562958E-5 12-13 5.123475765959626E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002002813253966036 22-23 0.0 24-25 0.00321381661682922 26-27 0.0014438886249522585 28-29 0.0023754296733085544 30-31 0.014904656773700732 32-33 5.123475765959626E-4 34-35 0.0021425444112194805 36-37 0.003912472403096442 38-39 0.00223569851605511 40-41 0.003726164193425183 42-43 0.0023288526208907396 44-45 3.2603936692470354E-4 46-47 1.3973115725344438E-4 48-49 1.3973115725344438E-4 50-51 1.8630820967125916E-4 52-53 5.589246290137775E-4 54-55 0.003120662511993591 56-57 2.7946231450688876E-4 58-59 9.781181007741105E-4 60-61 7.918098911028515E-4 62-63 0.0 64-65 9.315410483562958E-5 66-67 4.657705241781479E-5 68-69 0.0 70-71 2.3288526208907394E-4 72-73 4.657705241781479E-5 74-75 3.2603936692470354E-4 76-77 6.520787338494071E-4 78-79 4.1919347176033316E-4 80-81 0.004937167556288368 82-83 0.001350734520116629 84-85 0.003074085459575776 86-87 0.0013973115725344437 88-89 0.0011644263104453698 90-91 2.3288526208907394E-4 92-93 0.0 94-95 1.8630820967125916E-4 96-97 0.0012575804152809994 98-99 0.0023288526208907396 100-101 6.986557862672219E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1073490.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.04203847204116 #Duplication Level Percentage of deduplicated Percentage of total 1 74.07978073594072 34.84863895380497 2 13.833758301089308 13.015363804255243 3 4.953997982862024 6.99138491100629 4 2.2987347687757356 4.325486777190672 5 1.246022399980863 2.930771683846241 6 0.7649857106703463 2.1591892339149705 7 0.49392537555828075 1.6264679563531017 8 0.37904002470360826 1.426465233964046 9 0.27582953894989776 1.1678025404705819 >10 1.4755050983497981 12.702544426589554 >50 0.11937766232934525 3.8671760690436825 >100 0.07045951409956806 5.813060653462408 >500 0.005189652417510813 1.7038523704794644 >1k 0.0027944282248135144 2.9551218893271076 >5k 0.0 0.0 >10k+ 5.988060481743245E-4 4.466673496291641 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20678 1.9262405797911486 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15668 1.4595385145646445 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11224 1.0455616726751065 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4003 0.3728958816570252 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3457 0.32203374041677146 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3373 0.3142087956105786 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3203 0.29837259778852154 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2944 0.2742456846360935 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2586 0.2408965151049381 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1883 0.1754091794054905 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1681 0.15659205022869332 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1650 0.1537042729787888 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1630 0.15184119088207623 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1435 0.13367614043912845 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1240 0.11551108999618069 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1203 0.1120643881172624 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1184 0.11029446012538542 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.8630820967125916E-4 0.0 20-21 0.0 0.0 0.0 3.726164193425183E-4 9.315410483562958E-5 22-23 0.0 0.0 0.0 7.918098911028514E-4 9.315410483562958E-5 24-25 0.0 0.0 0.0 0.0019096591491304066 9.315410483562958E-5 26-27 0.0 0.0 0.0 0.003074085459575776 9.315410483562958E-5 28-29 0.0 0.0 0.0 0.005402938080466516 9.315410483562958E-5 30-31 0.0 0.0 0.0 0.010107220374665809 9.315410483562958E-5 32-33 0.0 0.0 0.0 0.014904656773700732 9.315410483562958E-5 34-35 0.0 0.0 0.0 0.019515784963064397 9.315410483562958E-5 36-37 0.0 0.0 0.0 0.025244762410455618 9.315410483562958E-5 38-39 0.0 0.0 0.0 0.03479305815610765 1.3973115725344438E-4 40-41 0.0 0.0 0.0 0.05374991849015827 1.8630820967125916E-4 42-43 0.0 0.0 0.0 0.06907376873561932 1.8630820967125916E-4 44-45 0.0 0.0 0.0 0.08640043223504644 1.8630820967125916E-4 46-47 0.0 0.0 0.0 0.12151952975807878 1.8630820967125916E-4 48-49 0.0 0.0 0.0 0.14755610205963726 1.8630820967125916E-4 50-51 0.0 0.0 0.0 0.17662018276835367 1.8630820967125916E-4 52-53 0.0 0.0 0.0 0.20838573251730338 1.8630820967125916E-4 54-55 0.0 0.0 0.0 0.2524476241045562 1.8630820967125916E-4 56-57 0.0 0.0 0.0 0.3587830347744273 1.8630820967125916E-4 58-59 0.0 9.315410483562958E-5 0.0 0.44294776849341866 1.8630820967125916E-4 60-61 0.0 1.8630820967125916E-4 0.0 0.5070377926203318 1.8630820967125916E-4 62-63 0.0 1.8630820967125916E-4 0.0 0.5829118110089522 1.8630820967125916E-4 64-65 0.0 1.8630820967125916E-4 0.0 0.6520321567969893 1.8630820967125916E-4 66-67 0.0 1.8630820967125916E-4 0.0 0.7697323682568072 1.8630820967125916E-4 68-69 0.0 1.8630820967125916E-4 0.0 0.9195707458849174 1.8630820967125916E-4 70-71 0.0 2.7946231450688876E-4 0.0 0.9979133480516819 1.8630820967125916E-4 72-73 0.0 2.7946231450688876E-4 0.0 1.0867823640648724 1.8630820967125916E-4 74-75 0.0 2.7946231450688876E-4 0.0 1.249382854055464 1.8630820967125916E-4 76-77 0.0 2.7946231450688876E-4 0.0 1.4229289513642418 1.8630820967125916E-4 78-79 0.0 2.7946231450688876E-4 0.0 1.58837064155232 1.8630820967125916E-4 80-81 0.0 3.726164193425183E-4 0.0 1.7385816355997727 1.8630820967125916E-4 82-83 0.0 3.726164193425183E-4 0.0 1.96457349393101 1.8630820967125916E-4 84-85 0.0 3.726164193425183E-4 0.0 2.3438970088216937 1.8630820967125916E-4 86-87 0.0 3.726164193425183E-4 0.0 2.6749666974075215 1.8630820967125916E-4 88-89 0.0 3.726164193425183E-4 0.0 3.0992836449338137 1.8630820967125916E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATC 20 0.0020725348 71.26304 1 GGTATCA 11885 0.0 32.81837 1 GTATCAA 17735 0.0 31.556377 1 TATCAAC 22140 0.0 25.249369 2 ATCAACG 22315 0.0 25.093924 3 TCAACGC 22490 0.0 24.919779 4 CAACGCA 22765 0.0 24.681341 5 GTACATG 10945 0.0 24.568222 1 TAAGGTG 505 0.0 24.453081 5 GAGTACT 13360 0.0 24.423164 12-13 AACGCAG 23220 0.0 24.218159 6 AGTACTT 13480 0.0 23.923876 12-13 TACATGG 11125 0.0 23.779726 2 GTAAGGT 435 0.0 22.92882 4 GTACTTT 14405 0.0 22.604053 14-15 TAATACC 275 5.2750693E-11 22.452377 4 AGGTAAG 360 0.0 22.428389 2 ACATGGG 11085 0.0 22.194517 3 ACGCAGA 25300 0.0 22.170784 7 CGCAGAG 25420 0.0 22.010067 8 >>END_MODULE